Polymer Films on Electrodes: XXII . Electrochemical, Spectroscopic, and Transmission Electron Microscopic Studies of Morphological Changes in Films of Polymeric Surfactants

1988 ◽  
Vol 135 (7) ◽  
pp. 1669-1675 ◽  
Author(s):  
Yu‐Min Tsou ◽  
Hsu‐Yang Liu ◽  
Allen J. Bard
Keyword(s):  
Polymer Films ◽  
Morphological Changes ◽  
Electron Microscopic ◽  
Polymeric Surfactants ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Microscopic Studies

Radial mass analysis of unstained STEM images of molluscan hemocyanins

1990 ◽  
Vol 48 (3) ◽  
pp. 810-811
Author(s):  
M.G. Hamilton ◽  
T.T. Herskovits ◽  
J.S. Wall
Keyword(s):  
Image Analysis ◽  
Hollow Cylinder ◽  
Electron Micrographs ◽  
Side View ◽  
Mass Analysis ◽  
Mass Measurements ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Microscopic Studies

The hemocyanins of molluscs are aggregates of a cylindrical decameric subparticle that assembles into di-, tri-, tetra-, penta-, and larger multi-decameric particles with masses that are multiples of the 4.4 Md decamer. Electron micrographs of these hemocyanins typically show the particles with two profiles: circular representing the cylinder viewed from the end and rectangular representing the side-view of the hollow cylinder.The model proposed by Mellema and Klug from image analysis of a didecameric hemocyanin with the two decamers facing one another with collar (closed) ends outward fits the appearance of side-views of the negatively-stained cylinders. These authors also suggested that there might be caps at the ends. In one of a series of transmission electron microscopic studies of molluscan hemocyanins, Siezen and Van Bruggen supported the Mellema-Klug model, but stated that they had never observed a cap component. With STEM we have tested the end cap hypothesis by direct mass measurements across the end-views of unstained particles.

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