scholarly journals Nutritive Value, Polyphenol Constituents and Prevention of Pathogenic Microorganism by Different Resin Extract of Commiphora myrrh

2020 ◽  
Vol 14 (3) ◽  
pp. 1871-1878
Author(s):  
Rasha Khalid Abbas ◽  
Amina A.M. Al-Mushhin ◽  
Fatima S. Elsharbasy ◽  
Kother Osman Ashiry
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Pathogenic Bacteria ◽  
Nutritive Value ◽  
Folk Medicine ◽  
Inhibition Zone ◽  
Syringic Acid ◽  
Total Fat ◽  
Bacteria And Fungi ◽  
Resin Extract

The resin extract of Commiphora myrrh is Widely used in the folk medicine. The studying myrrh resin extract include moisture. minerals such as (Ca, Fe, Mg, Na, Cu and Zn), protein, total fat and crude fiber. In this study used Muffle furnace, Kjeldahl methods Soxlet and atomic absorption. HPLC using to evaluating Polyphenol constituents of myrrh different resin extract (ethanol, ethyl acetate, petroleum ether and chloroform) as Conc. (µg / g) and in all extract (ethanol, ethyl acetate and petroleum ether and chloroform) it contained Chlorogenic acid, gallic acid Catechin, Coffeic acid, caffeine, Syringic acid, Coumaric acid, Ferulic acid, Naringenin, 4`.7-Dihydroxyisoflavone, Cinnamic, Propyl Gallate Vanillin, Querectin and Acid Ellagic acid in different concentration percentage and area The effect of Commiphora myrrh (ethanol, ethyl acetate, petroleum ether and chloroform) resin extract against four different pathogenic bacteria Salmonella typhimurium, Pseudomona aeruginosa, Escherichia coli, and Bacillus cereus, were examine by Mueller Hinton Agar and measuring inhibition zone (diameter mm), show that there were significant different among bacteria and different method of extract. All different Commiphora myrrh seed extract (aqueous, ethyl acetate and petroleum ether) have high activity against Candida albicans fungus. The study was conducted to identified the Commiphora myrrh nutritive value, polyphenol Compound and the activity against bacteria and fungi.

Antimicrobial Activity of Coleus forskohlii Fractions, Isolation and Characterization of Phenolic Compounds

2021 ◽  
Vol 17 ◽  
Author(s):  
Kamel H. Shaker ◽  
M. Abo Yonus ◽  
Mohamed A. Ibrahim ◽  
Mona Kilany ◽  
Frank Wiggers
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Fraction ◽  
Antimicrobial Activities ◽  
Syringic Acid ◽  
Coleus Forskohlii ◽  
Isolation And Characterization ◽  
Asir Region

Background: It is known that medicinal plants represent promising candidates against many species of pathogenic bacteria. The south area of Saudi Arabia "Asir region" has a unique habitat and its medicinal plant's composition is still nearly unexplored. Therefore, the aim of the present work is to investigate the antimicrobial activities of Coleus forskohlii fractions and to identify the major active compounds. Methods: The total plant extract was partitioned by petroleum ether, ethyl acetate and n-butanol. Agar well diffusion was assessed to evaluate the antimicrobial activities of the plant fractions against Gram-positive, Gram-negative and Candida albicans. Successive column chromatography was performed to isolate the major metabolites. Structures of the isolated compounds were determined by nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. Results: All plant fractions showed significant antimicrobial potential activities against the tested pathogens, where ethyl acetate exhibited the highest antimicrobial activity followed by petroleum ether then n-butanol. From n-butanol fraction, thymoquinol-2-O-β-glucopyranoside (1) was isolated while syringic acid (2), methyl 3,4,5-trihydroxybenzoate (3), and luteolin (4) were assigned from the ethyl acetate fraction. Conclusion: The antimicrobial assays revealed that ethyl acetate was the most potent fraction and the major abundant metabolites of C. forskohlii, thymoquinol-2-O-β-glucopyranoside (1), syringic acid (2), methyl 3,4,5-trihydroxybenzoate (3), and luteolin (4) were isolated herein for the first time.

scholarly journals UJI EFEKTIVITAS DAUN PANDAN WANGI (PANDANUS AMARYLLIFOLIUS ROXB.) SEBAGAI ANTIBAKTERI TERHADAP SALMONELLA TYPHI

2019 ◽  
Vol 6 (1) ◽  
pp. 59
Author(s):  
Priska Nancy Claudia Bali ◽  
Ahmad Raif ◽  
Setia Budi Tarigan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Salmonella Typhi ◽  
Inhibition Zone Diameter ◽  
Negative Controls ◽  
Pandanus Amaryllifolius ◽  
Positive Controls

Pandanus amaryllifolius roxb leaves commonly used as food dditives which contain various compound groups namely alkaloid, saponins, flavonoids and tannins that have a role in antibacterial activity. This research is indicated to support the success of antibacterial in fragrant pandanus leaf plants against Salmonella typhi, one of the pathogenic bacteria. The antibacterial effectiveness test of ethanol-ethyl acetate extract (1: 1) of pandanus leaves which evaluated by the Kirby-bauer method and making the extract concentrations of 25%, 50%, 75%, 100%. The results showed that pandanus leaves extract had antibacterial effectiveness against Salmonella typhi with an average inhibition zone diameter of 11.6 mm; 14 mm; 14.3 mm; 15.3 mm; positive controls (Ciprofloxacin) 27 mm; and negative controls (aquadest) didn’t produce the inhibition zone of Salmonella typhi.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

Ethnomedicinal Survey of Antidiabetic Plants and Preliminary Evaluation of Antioxidant, Hypoglycemic Activity Maesa indica Leaves, a Folk Antidiabetic Plant of Manipur, India

2020 ◽  
Vol 06 ◽  
Author(s):  
Raja Chakraborty ◽  
Saikat Sen ◽  
Nongmaithem Randhoni Chanu ◽  
Akoijam Bishaljit Singh ◽  
Crescent Lyngkhoi ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Ethanol Extract ◽  
Hypoglycemic Activity ◽  
Ether Extract ◽  
Ic50 Value ◽  
Nitric Oxide Radical ◽  
Antidiabetic Plants

Background: Manipur is a small state of India, well known for its folk medicine. Folk medicine practitioners of the state use a number of plants to manage diabetes mellitus. Objective: To carry out an ethnomedicinal survey to find folk antidiabetic plants of Thoubal district of Manipur, India. This study was aimed to investigate the antioxidant and hypoglycemic potential of chosen medicinal plant. Methods: An ethnomedicinal survey was carried out in Thoubal district, Manipur to find out antidiabetic plants regularly used by tribal/folk medicinal practitioners. Based on the information, Maesa indica leaves extracts (ethanol extract, MIE; ethyl acetate extract, MIEA; petroleum ether extract, MIPE) further investigated for in vitro antioxidant and antidiabetic activity. Acute hypoglycemic activity (streptozotocin-induced diabetes model) and NG-OGTT tests were employed to evaluate the hypoglycemic activity of Maesa indica leaves. Results: Ethnomedicinal survey revealed that folk medicinal practitioners regularly used 15 antidiabetic plants. Maesa indica is a plant of choice of folk medicinal practitioners. MIE was found to possess strong oxidative haemolysis inhibition, nitric oxide radical and DPPH radical scavenging effect with IC50 value of 54.5, 34.1 and 19.2 μg/mL respectively. MIE inhibited α-glucosidase and α-amylase with IC50 value 18.2 and 31.2 μg/mL respectively. MIE and MIEA produced considerable hypoglycemic activity when tested through NG-OGTT model. MIE (500 mg/kg, b.w.) asserted significant acute hypoglycemic activity by reducing blood glucose level after 1h, 2h, 3h, 5h, and 12h of drug administration in streptozotocin-induced diabetic rats. Conclusion: The present study showed that ethanol extract of Maesa indica leaves possesses better activity compare to ethyl acetate and petroleum ether extract. This study also confirmed the ethnobotanical claim about traditional use of plant and antidiabetic potential of M. indica leaves.

scholarly journals Antimicrobial activity of some Bacillus spp. Separated from the soil

2019 ◽  
Vol 10 (2) ◽  
pp. 1460-1463
Author(s):  
Zhara Tareq Abdulhameed Baqqal ◽  
Rafal Mhaidi Younus ◽  
Saja Dhyaaldain Mustafa
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Effect Of Temperature ◽  
Good Growth ◽  
Candida Spp ◽  
Gram Positive ◽  
Bacillus Spp ◽  
Bacillus Genus ◽  
Bacteria And Fungi

In this study select two active isolate from nine isolates of local agricultural soil of Mosul, were collected from 10 sample of 6 different region depending on antimicrobial activity to some test bacteria and fungi, Identified as gram-positive rod-shaped bacteria and, biochemical test, the selected isolates belonged to Bacillus genus, gave signal B1 and B2 were effective against both pathogenic bacteria Gram-positive and Gram-negative, two isolates gave high inhibition zone about 23mm against Staphylococcus aureus whereas gave less inhibition zone against Escherichia .coli, Klebsiella spp, Aspergillus niger and Candida spp, 20, 16, Furthermore studied the effect of temperature on the growth of two isolates which gave good growth at 40-45 degree whereas there was no growth at low temperature 5°C. Furthermore studied the effect of salt on the growth of two isolates that gave good growth at concentration 3% NaCl; the two strains were analysed utilizing agar publishing way and cross streak method.

scholarly journals Investigating the Inhibitory Effect of Silver Nanoparticles against Some Species of Candida and Pathogenic Bacteria

2020 ◽  
Vol 4 (1) ◽  
pp. 32-36
Author(s):  
Alaa M. Hasan ◽  
Sura M. Abdul Majeed ◽  
Rusol M. Al-Bahrani
Keyword(s):  
Escherichia Coli ◽  
Silver Nanoparticles ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Candida Guilliermondii ◽  
Candida Krusei ◽  
Maximum Inhibition ◽  
Bacteria And Fungi ◽  
Candida Zeylanoides

Silver nanoparticles synthesized from aqueous extract of mushroom Pleurotus ostreatus exhibited inhibitory effect at the concentration of 12.5, 25, 50, and 100 mg/ml against some pathogenic bacteria and fungi such as Candida albicans, Candida guilliermondii, Candida krusei, Candida zeylanoides, Geotrichum klebahnii, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. The maximum inhibition zone was observed against C. zeylanoides at the concentration of 100 mg/ml was 24.5 mm, while the minimum inhibition zone was observed against Geotrichum at the concentration of 25 mg/ml was 8 mm and the concentration of 12.5 mg/ml was not effective against some species.

scholarly journals Antibacterial Activity of the Red Algae Eucheuma cottonii Extract from Tanjung Coast, Sumenep Madura

ALCHEMY ◽  
2016 ◽  
Vol 4 (2) ◽  
pp. 93 ◽  
Author(s):  
Zulli Andriani ◽  
Ahmad Ghanaim Fasya ◽  
Ahmad Hanapi
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Red Algae ◽  
Methanol Extract ◽  
Petroleum Ether Extract ◽  
Inhibition Zone ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Eucheuma Cottonii

<p align="center"><strong>Abstract</strong></p><p>Red alge <em>Euchema cottonii</em> is one of the biological source that has benefits. The aim of this study is to determine the effectiveness of antibacterial red algae <em>Eucheuma cottonii</em> extract<em>. </em>Isolation of active compounds was performed by maceration method using methanol. The methanol extract was hydrolyzed using HCl 2 N and partitioned by ethyl acetate, chloroform, petroleum ether and <em>n</em>-hexane solvents. The antibacterial assessment was determined using disc diffusion method againts bacteria <em>Staphylococcus aureus </em>and <em>Escherichia coli</em>. Identification of active compound was conducted by reagents test. The results showed that the best solvent to extract algae was methanol with 20.7% rendemen. The methanol extract 4% gives the highest inhibition at concentrations was 7.85 mm; followed by <em>n</em>-hexane and chloroform were 1.0 and 0.6 mm. However, bath ethyl acetate and petroleum ether extract depicted no inhibition zone. The identification test showed the presence of flavonoids, triterpenoids, steroids and alkaloids in the methanol extract. The extract of n-hexane contained triterpenoids and alkaloids, while the chloroform extract contained flavonoids, triterpenoids and alkaloids.</p><p> </p><p><strong>Keyword</strong><strong>s</strong>:  <em>Euchema cottonii</em>, antibacterial, disc diffusion<em></em></p>

scholarly journals STUDY OF ANTIMICROBIAL ACTIVITY OF THE FOLKLORE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2017 ◽  
Vol 4 (2) ◽  
pp. 57-60
Author(s):  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Rhizopus Sp

The purpose of the study is to examine the antimicrobial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa Forssk by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the methanol extracts exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis and ethyl acetate extracts showed higher inhibiotion zone (24.83 mm) against the fungus, Rhizopus sp. Results concluded that this spices contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preservater and it can also use for medicinal purpose.

scholarly journals ANTIMICROBIAL ACTIVITY OF THE FOLKLORE MEDICINAL PLANT, ACACIA CAESIA (L.) WILD.

2016 ◽  
Vol 3 (1) ◽  
pp. 49-52
Author(s):  
Thambiraj J ◽  
Paulsamy S
Keyword(s):  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Mucor Rouxii

The aim of this study is to evaluate the antimicrobial efficacy of stem bark extracts of the folklore plant species, Acacia caesia L. by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (17.23 mm) against the bacterium, Klebsiella pneumoniae and the fungus, Mucor rouxii (30.77 mm). These results support the therapeutic importance of the species, Acacia caesia in curing infectious diseases and encourage the extensive use of this species in health care practices

scholarly journals SCREENING OF ANTIMICROBIAL ACTIVITY IN PENTATROPIS MICROPHYLLA (ROTH) WIGHT (APOCYNACEAE)

2017 ◽  
Vol 4 (2) ◽  
pp. 127-129
Author(s):  
Prema R ◽  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method

The stem bark extracts of the medicinal plant species, Pentatropis microphylla Roth Wight by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against four human pathogenic bacteria viz., Bacillus subtilis, B. thuriengensis, Klebsiella pneumoniae and Escherichia coli and fourhuman pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii and Fusarium oxysporum for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (17.23 mm) against the bacterium, Klebsiella pneumoniae and the fungus, Aspergillus niger (19.63 mm). Therefore the result strengthens the existing traditional usage of the plant for the therapeutic use.

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