scholarly journals STUDY OF ANTIMICROBIAL ACTIVITY OF THE FOLKLORE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2017 ◽  
Vol 4 (2) ◽  
pp. 57-60
Author(s):  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Rhizopus Sp

The purpose of the study is to examine the antimicrobial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa Forssk by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the methanol extracts exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis and ethyl acetate extracts showed higher inhibiotion zone (24.83 mm) against the fungus, Rhizopus sp. Results concluded that this spices contain high amount of secondary metabolites due to these metabolites they have high antimicrobial activity and it can be used as good bio- preservater and it can also use for medicinal purpose.

scholarly journals ANTIMICROBIAL EFFICACY OF THE FOLKLORE MEDICINAL PLANT, ACACIA CAESIA (L.) WILD

2015 ◽  
Vol 2 (2) ◽  
pp. 110-113
Author(s):  
Thambiraj J ◽  
Paulsamy S
Keyword(s):  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Mucor Rouxii ◽  
Bacterium Bacillus ◽  
Rhizopus Sp

eaf extracts of the folklore plant species, Acacia caesia L. by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (15.73 mm) against the bacterium, Bacillus subtilis and the fungus, Mucor rouxii (20.67 mm). These results support the therapeutic importance of the species, Acacia caesia in curing infectious diseases and encourage the extensive use of this species in health care practices.

scholarly journals ANTIMICROBIAL ACTIVITY OF THE FOLKLORE MEDICINAL PLANT, ACACIA CAESIA (L.) WILD.

2016 ◽  
Vol 3 (1) ◽  
pp. 49-52
Author(s):  
Thambiraj J ◽  
Paulsamy S
Keyword(s):  
Antimicrobial Activity ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method ◽  
Mucor Rouxii

The aim of this study is to evaluate the antimicrobial efficacy of stem bark extracts of the folklore plant species, Acacia caesia L. by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri, Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae and ten human pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii, Fusarium oxysporum, F. solani, Mucor rouxii, Alternaria alternata, Candida albicans, Cladosporium sp. and Rhizopus sp. for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (17.23 mm) against the bacterium, Klebsiella pneumoniae and the fungus, Mucor rouxii (30.77 mm). These results support the therapeutic importance of the species, Acacia caesia in curing infectious diseases and encourage the extensive use of this species in health care practices

scholarly journals SCREENING OF ANTIMICROBIAL ACTIVITY IN PENTATROPIS MICROPHYLLA (ROTH) WIGHT (APOCYNACEAE)

2017 ◽  
Vol 4 (2) ◽  
pp. 127-129
Author(s):  
Prema R ◽  
Thambiraj J
Keyword(s):  
Antimicrobial Activity ◽  
Aspergillus Niger ◽  
Klebsiella Pneumoniae ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Antimicrobial Properties ◽  
Pathogenic Fungi ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Diffusion Method

The stem bark extracts of the medicinal plant species, Pentatropis microphylla Roth Wight by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against four human pathogenic bacteria viz., Bacillus subtilis, B. thuriengensis, Klebsiella pneumoniae and Escherichia coli and fourhuman pathogenic fungi viz., Aspergillus niger, A. flavus, A.baumannii and Fusarium oxysporum for assessing the antimicrobial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antimicrobial activity against the tested pathogens. However, the ethyl acetate extracts exhibited higher inhibition zone (17.23 mm) against the bacterium, Klebsiella pneumoniae and the fungus, Aspergillus niger (19.63 mm). Therefore the result strengthens the existing traditional usage of the plant for the therapeutic use.

scholarly journals ANTIBACTERIAL ACTIVITY OF THE MEDICINAL PLANT, ACALYPHA FRUTICOSA FORSSK.

2016 ◽  
Vol 3 (2) ◽  
pp. 72-74
Author(s):  
Thambiraj J
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Medicinal Plant ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Root Extracts ◽  
Bacterium Bacillus Subtilis ◽  
Bacterium Bacillus

The present study is to confirm the antibacterial efficacy of root extracts of the folklore medicinal plant species, Acalypha fruticosa by using three alcoholic solvents viz; petroleum ether, ethyl acetate and methanol were tested against ten human pathogenic bacteria viz., Pseudomonas aeruginosa, P. stutzeri,Escherichia coli, Micrococcus sp., Lactobacillus sp., Servatia sp., Moraxetta sp., Bacillus subtilis, B. thuriengensis, and Klebsiella pneumoniae for assessing the antibacterial properties by adapting disc diffusion method. The results of the study revealed that all extracts showed varied degree of antibacterial activity against the tested pathogens. However, the methanol extract exhibited higher inhibition zone (21.83 mm) against the bacterium, Bacillus subtilis. This result supports the therapeutic importance of the species, Acalypha fruticosa in curing infectious diseases and encourages the extensive use of this species in health carepractices.

scholarly journals ANTIMICROBIAL ACTIVITY OF SELECTED INDIAN FOLK MEDICINAL PLANTS: MYRISTICA FATUA, ALSTONIA BOONEI, HELICTERES ISORA, VITEX ALTISSIMA AND ATALANTIA RACEMOSA

2017 ◽  
Vol 10 (2) ◽  
pp. 277
Author(s):  
Kokkaiah Irulandi ◽  
Sethupandian Geetha ◽  
Palanichamy Mehalingam
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Inhibitory Effect ◽  
Diffusion Method ◽  
Alternative Source ◽  
Helicteres Isora ◽  
Acetone Extracts

Objective: To determine antimicrobial activity of methanol, ethyl acetate and acetone extracts of Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa  against different species of pathogens, Streptococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Staphyllococcus aureus, and Candida albicans.Methods: Antimicrobial activity of plant extracts was measured by agar well diffusion method.Results: Acetone extracts of Alstonia boonei showed the highest inhibitory effect against Escherichia coli (21.00 ± 1.00 mm) and Streptococcus faecalis (19.00 ± 1.00 mm). All the extracts of Heliteres isora leaves showed different zone of inhibition observed in all the tested pathogens ranges between (8.13 ± 1.53 – 15.25 ± 1.23 mm). Ethyl acetate extract of Vitex altissima showed highest activity against Bacillus sublilis (19.67 ± 1.53 mm). Methanol and acetone leaves extracts of Atalantia racemosa have good fungal activity against the Candida albicans (19.33 ± 1.26 mm - 16.00 ± 1.00 mm). Methanol extract of Myristica fatua showed high antimicrobial activity against Pseudomonas aeruginosa (15.10 ± 0.17 mm) and Bacillus subtilis (14.23 ± 0.21 mm).Conclusion: The results from the study suggest that the leaves Myristica fatua, Alstonia boonei, Helicteres isora, Vitex altissima and Atalantia racemosa showed good antimicrobial activity against the different pathogens. They are used as the alternative source for the control and treatment of microbial infections.Keywords: Antimicrobial activity, Leaves extracts,  Well diffusion method, Pathogenic strains.

scholarly journals Combined activity of Azadirachta indica and Dodonea viscose leaves extract on some selected pathogens

2020 ◽  
Vol 12 (1) ◽  
pp. 31-38
Author(s):  
A. I Isah ◽  
A.I. Aminu
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Azadirachta Indica ◽  
Pathogenic Bacteria ◽  
Bacterial Species ◽  
Plant Secondary Metabolites ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Plant Materials

The study was undertaken to investigate the antimicrobial activity both individually and in combination of two Nigerian plants (Azadirachta indica and Dodonea viscosa) against a variety of pathogenic bacteria. Five bacterial species namely; Staphylococcus aureus, Escherichia coli, Salmonella typhi, Klebsiella pneumoniae and Pseudomonas aeruginosa were selected for the assays. The powdered plant materials of the two plants were extracted using sohxlet extraction technique with methanol water and petroleum ether as solvents. The crude extracts of the two plants were subjected to phytochemical screening for qualitative detection of plant secondary metabolites. The extracts were further tested for antibacterial activity against the selected pathogens singly and then combined using agar well diffusion method. Antibacterial activity of the two plants singly indicates that the methanolic extract possess the highest antibacterial activity at a concentration of 50mg/ml with an inhibition zone of 23.3±0.5mm compared to aqueous extract with 21.7±0.5mm inhibition zone at a concentration of 50mg/ml. The least activity was observed with the petroleum ether extract with an inhibition zone of 7.3±0.5mm at a concentration of 12.5mg/ml. Combination of the plants extracts exhibited lower antibacterial activity on the test isolates compared to single plants as evidenced by the production of lower inhibition zones. Key words: Azadirachta indica, Dodonea viscosa, pathogens, antimicrobial activity, synergistic effect

scholarly journals Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis

2017 ◽  
Vol 18 (4) ◽  
pp. 312-316 ◽  
Author(s):  
Aarti A Bohora ◽  
Sharad R Kokate
Keyword(s):  
Antimicrobial Activity ◽  
Enterococcus Faecalis ◽  
Pathogenic Bacteria ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Indicator Strain ◽  
Oral Bacteria ◽  
Diffusion Method

ABSTRACT Introduction The main goal of endodontics is the prevention of apical periodontitis. This is due to the presence of persistent pathogenic microorganisms, such as Enterococcus faecalis, and its ability to directly cause acute and chronic inflammation in the periapical tissues. Lactobacillus has been shown to promote health in the intestines as well as to inhibit the growth of certain problematic oral bacteria. This study explores shifting the established paradigm of endodontic treatment, which has focused on eliminating all bacteria from the canal system and on elimination of the problematic bacteria through introducing probiotics. A preliminary work was performed to evaluate the possible effectiveness of probiotics in preventing the growth of E. faecalis. Materials and methods Two probiotic cultures Lactobacillus plantarum ATCC 8041 and Lactobacillus rhamnosus ATCC 7408 were selected to check their antimicrobial activity against E. faecalis ATCC 29212 by two methods: agar cup/well diffusion method and deferred antagonism test. Agar cup method A total of 0.5 mL of requisite test pathogen culture was inoculated into 20 mL of molten sterile Mueller and Hinton agar and cooled to 45 ± 2°C. Circular wells of diameter 10 mm were punched in each of the poured plates. Appropriately diluted test samples were added to the above-punched wells. The plates were incubated upright position at 37°C for 24 hours in aerobic conditions. Postincubation, zone of inhibition was measured. The cell-free supernatant of Lactobacillus species was also evaluated for antimicrobial activity. Deferred antagonism test The test probiotic strain was standardized to 0.1 optical density (OD) at 600 nm and inoculated in a 1 cm wide diametric streak across the surface of trypticase soy agar + yeast extract + calcium carbonate (TSYCa) agar using a sterile cotton swab. Then, the plate agar containing the test strain was incubated at 37°C for 24 hours under aerophilic condition and then standardized to 0.1 OD at 600 nm for overnight (18 hours, 37°C). A purified culture of indicator strain (pathogen) was streaked at right angles to the line of original producer growth. Postincubation plates were observed for the inhibition zone width of the indicator strain. Results Under the conditions of this study, Lactobacilli had an inhibitory effect on the growth of E. faecalis by agar cup method but not by deferred antagonism test. Conclusion This pilot study demonstrated that probiotics show a potential in root canal therapy. Clinical Significance If probioitcs are effective against endodontic pathogens, they can be potentially used as intracanal medicaments. This will be a novel concept of introducing bacteriotherapy in endodontics and replacing pathogenic bacteria by healthy bacteria, normal flora. How to cite this article Bohora AA, Kokate SR. Good Bugs vs Bad Bugs: Evaluation of Inhibitory Effect of Selected Probiotics against Enterococcus faecalis. J Contemp Dent Pract 2017;18(4):312-316.

scholarly journals POTENSI BAKTERI ASAM LAKTAT DALAM MENGHASILKAN BAKTERIOSIN SEBAGAI ANTIMIKROBA DAN PENGUKURAN BERAT MOLEKULNYA DENGAN SDS-PAGE DARI ISOLAT FERMENTASI KAKAO

2015 ◽  
Vol 1 (2) ◽  
pp. 126
Author(s):  
Urnemi ◽  
Sumaryati Syukur ◽  
Endang Purwati ◽  
Sanusi Ibrahim ◽  
Jamsari
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Inhibition Zone ◽  
Sds Page ◽  
Inhibition Zones ◽  
And Staphylococcus Aureus

ABSTRACT Lactic acid bacteria (LAB) were isolated from of cocoa beans fermentation Forestero variety from West Sumatera, that were eleven isolates. The isolates were tested to antimicrobial activity against pathogenic bacteria E.coli NBRC 14237, Staphylococcus aureus NBRC 13276, Bacillus subtilis BTCCB 612, listeria m. dan S. Typhii. Results the research showed that, isolates had inhibition zone to pathogenic bacteria, that were 7 mm till 12 mm at 48 hours observation. R2.4 isolate was most potential to inhibition zones growth pathogenic bacteria, that was 11mm till 12 mm to five pathogens. R2.4 isolates was the highest to against pathogenic bacteria (Bacillus subtilis BTCCB, Listeria monocytogenesis and Staphylococcus aureus NBRC) had inhibition zones, that was 12.00 mm till 48 hours. Listeria monocytogenesis had been known as pest bacterium of food born, so that R2.4 isolate can be used as food biopreservative. Crude of R2.4 isolate molecular weight was 10 kDa by SDS-PAGE.  Key words: Lactic acid bacteria, Antimicrobial activity, SDS-PAGE, Cocoa fermentation and food biopreservative                                                      

scholarly journals <i>In-vitro</i> antibacterial sensitivity of <i>Usnea barbata</i> lichen extracted with methanol and ethyl-acetate against selected <i>Staphylococcus</i> species from milk of cows with mastitis

2014 ◽  
Vol 57 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Emrobowansan Monday Idamokoro ◽  
Patrick Julius Masika ◽  
Voster Muchenje ◽  
Daniel Falta ◽  
Ezekiel Green
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Bovine Milk ◽  
Raw Milk ◽  
Diffusion Method ◽  
Biochemical Tests ◽  
Ethyl Acetate Extracts

Abstract. This study aimed at evaluating the antimicrobial potential of Usnea barbata lichen as a medicinal plant against selected Staphylococcus species isolated from raw milk of cows. In-vitro screening of methanol and ethyl-acetate extracts from Usnea barbata lichen were evaluated to determine their antimicrobial activity against thirteen different Staphylococcus species. The selected organisms were isolated from raw bovine milk and identified using several biochemical tests and confirmed with API staph kit. The antimicrobial activity of the extracts were evaluated using both the agar well diffusion method (at 5 mg/ml, 10 mg/ml and 20 mg/ml) and the broth micro-dilution technique to determine the mean zone of inhibition and the minimum inhibitory concentration (MIC), respectively. Both the methanol and ethyl-acetate extracts showed variable antimicrobial activity against the Staphylococcus species with mean zones of inhibition ranging from 0-34 mm in diameter at 5 mg/ml, 10 mg/ml and 20 mg/ml, respectively. Susceptibility by the Staphylococcus species tested in the methanol and the ethyl-acetate extract was 92.31 % and 53.85 %, respectively. The MIC result for the methanol extract ranged from 0.04 to 10 mg/ml, while that of the ethyl-acetate extract ranged from 0.16 to 5 mg/ml. Results from this study revealed the in vitro microbial activity of Usnea barbata extracts which indicate its potential as a medicinal plant.

In vitro antimicrobial activity and phytochemical analysis of fruits of Syzygium aromaticum (L.) Merr. & L.M.Perry - An important medicinal Plant

2016 ◽  
Vol 5 (4) ◽  
pp. 137-140
Author(s):  
Duraisamy Kayal Vizhi ◽  
◽  
Kokkaiah Irulandi ◽  
Palanichamy Mehalingam ◽  
Natarajan Nirmal Kumar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Syzygium Aromaticum ◽  
Active Components ◽  
Methanol Extracts ◽  
Important Medicinal Plant

The fruit of Syzygium aromaticum is an important medicinal plant was subjected to phytochemical screening and antimicrobial effect of methanol, ethyl acetate and acetone extracts. Antimicrobial activity was analyzed by agar well diffusion method against gram positive and gram negative bacteria. The phytochemical analysis carried out revealed the presence of alkaloids, coumarins, catechins, flavanoids, phenols, saponins, terpenoids, tannins and steroids in various solvent extracts. Methanol extract of S. aromaticum showed good antimicrobial activity against Bacillus subtilis, Pseudomonas aeruginosa and Staohylococcus aureus. It has been showed that the methanol extracts had wider range of activity on these organisms than the acetone and ethyl acetate extracts, which indicates that the methanol extracts of selected plants may contain the active components. The use of plant extracts with known antimicrobial properties can be of great significance in therapeutic treatments.

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