scholarly journals Isolation and Characterization of Lytic Coliphages from Sewage Water

2017 ◽  
Vol 11 (1) ◽  
pp. 559-565 ◽  
Author(s):  
PU Megha ◽  
S Murugan ◽  
PS Harikumar
Keyword(s):  
Sewage Water ◽  
Isolation And Characterization

scholarly journals ISOLATION AND CHARACTERIZATION OF PHENOL DEGRADING BACTERIA FROM INDUSTRIAL WASTEWATER AND SEWAGE WATER

2021 ◽  
Vol 48 (2) ◽  
pp. 443-457
Author(s):  
Salma Salem ◽  
H. Abd El‌‌‌-Fattah ◽  
Howaida Abdelbasit ◽  
S. Mahgoub
Keyword(s):  
Industrial Wastewater ◽  
Sewage Water ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and Characterization of Multiple Drug Resistant Human Enteric Pathogens from Sewage Water of Delhi

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Rudrangshu Chatterjee ◽  
Dushyant Singh ◽  
Swati Tripathi ◽  
Abhishek Chauhan ◽  
M.L Aggarwal ◽  
...  
Keyword(s):  
Sewage Water ◽  
Enteric Pathogens ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Isolation And Characterization ◽  
Multiple Drug Resistant

scholarly journals Isolation and Characterization of Lytic Bacteriophages Infecting Acinetobacter baumannii

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ziad M.F. Al-Khozai ◽  
Mohammed N.H. Al-Kaabi
Keyword(s):  
Acinetobacter Baumannii ◽  
Burst Size ◽  
Sewage Water ◽  
Optimal Temperature ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Lysis Time ◽  
Wide Range ◽  
Complete Lysis

This study was carried out during the period from October 2015 to March 2016. It included the isolation of 42 isolates were identified asAcinetobacterbaumannii. All Isolates showed wide range of resistance to antibiotics. Two types of lytic bacteriophages primarily named Phage 1 and Phage 2 were isolated from sewage water. Phage 1 exhibited broad host spectrum it was lytic against all Acinetobacter baumannii isolates included for the study. Conversely,phage 2 have narrow host range as they could inhibit only 30 isolates,None of these phages inhibited a bacterium other than staphylococci species. The adsorption rate of Phage 1 was 3.1×10-10 ml min-1while Phage 2 was 1.7×10-10 ml min-1. Eclipse and Latent periods of Phage 1 were 6,17 minutes and for phage 2 were 7,14 minutes respectively,the burst size of Phage 1 and Phage 2 were 73±16 pfu\cell and 38±11 pfu\cell respectively. Complete lysis time of Phage 1 particles was 4.30 hours while the complete lysis time of Phage 2 particles was 6 hours. Phages passaging results showed a remarkable increment in PFU which were reached its maximal elevation at six passage in Phage 1 and fourth passage in Phage 2. Fitness of Phage 1 and Phage 2 were 24 ±5 pfu\cell and 26 ±4 pfu\cellrespectively through six generations. The Phage 1 and Phage 2 particles were stabled at a wide range of pH (6-10) and temperatures (30-50°C),the optimal temperature of two phages were 37°C.

Isolation and Characterization of Lytic Bacteriophages infecting Staphylococcus epidermidis

2016 ◽  
Vol 2 (1) ◽  
pp. 8-11
Author(s):  
Ream Saleem ◽  
Ghanim Aboud Jaber Al-Mola
Keyword(s):  
Staphylococcus Epidermidis ◽  
Plaque Assay ◽  
Sewage Water ◽  
Effect Of Temperature ◽  
Biochemical Tests ◽  
Phage Titer ◽  
Isolation And Characterization ◽  
Bacterial Lawn ◽  
Optimal Ph

The Staphylococcus epidermidis isolates were obtained during a period extended between September 2014 and January 2015, depending on biochemical tests and VITEK 2 system. Several sewage water samples were assayed using a plaque assay of double agar overlay as a source of S. Epidermidis phages. The bacteriophages were described depending on plaques size and shapes. Phage 1 was the most predominant in the bacterial lawn and able to infect other S. species such as S. aureus. Therefore, it was decided to study the effect of temperature on its original titer. The results revealed a gradual decrease in the phage titer with increasing dilution number. Each temperature at several incubation periods, significantly vary depending on phage titer. The optimum temperature was 40 ° C, while the 80 ° C was represented the inhibitor temperature. L.S.D. at level (0.05) for interaction was 39.552. The pH 6.5 – 7.5 were represented the optimal pH for the best phage activity while the phage titer beginning to decline in above and below this range of optimal pH , L.S.D. at level 0.05 was 17.898. In conclusion, our study found that Phage1 was considered as predominant phage because of their ability to infect other Staphylococci species such as S. Aureus.

Isolation and characterization of drug resistantSalmonella typhifrom sewage water

2015 ◽  
Vol 8 (2) ◽  
pp. 167 ◽  
Author(s):  
Vanka Kanth Swaroop ◽  
Aparna Mukherjee ◽  
Sumit Sharma ◽  
William Jabez Osborne
Keyword(s):  
Sewage Water ◽  
Isolation And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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