Isolation and characterization of an antibacterial compound producing Stenotrophomonas strain from sewage water, production optimization, and its antibiotic potential evaluation

2021 ◽  
Author(s):  
Debapriya Sarkar ◽  
Soumyashree Nanda ◽  
Kasturi Poddar ◽  
Angana Sarkar
Keyword(s):  
Sewage Water ◽  
Production Optimization ◽  
Water Production ◽  
Isolation And Characterization ◽  
Antibacterial Compound ◽  
Potential Evaluation

Isolation and characterization of novel antibacterial compound from an untapped plant, Stereospermum fimbriatum

2018 ◽  
Vol 34 (5) ◽  
pp. 629-637 ◽  
Author(s):  
Anis Fadhlina Izyani Awang ◽  
Qamar Uddin Ahmed ◽  
Syed Adnan Ali Shah ◽  
Juliana Md. Jaffri ◽  
Kashif Ghafoor ◽  
...  
Keyword(s):  
Isolation And Characterization ◽  
Antibacterial Compound

scholarly journals ISOLATION AND CHARACTERIZATION OF PHENOL DEGRADING BACTERIA FROM INDUSTRIAL WASTEWATER AND SEWAGE WATER

2021 ◽  
Vol 48 (2) ◽  
pp. 443-457
Author(s):  
Salma Salem ◽  
H. Abd El‌‌‌-Fattah ◽  
Howaida Abdelbasit ◽  
S. Mahgoub
Keyword(s):  
Industrial Wastewater ◽  
Sewage Water ◽  
Isolation And Characterization ◽  
Degrading Bacteria

scholarly journals Isolation and Characterization of Multiple Drug Resistant Human Enteric Pathogens from Sewage Water of Delhi

2021 ◽  
Vol 20 (2) ◽  
Author(s):  
Rudrangshu Chatterjee ◽  
Dushyant Singh ◽  
Swati Tripathi ◽  
Abhishek Chauhan ◽  
M.L Aggarwal ◽  
...  
Keyword(s):  
Sewage Water ◽  
Enteric Pathogens ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Isolation And Characterization ◽  
Multiple Drug Resistant

Bioassay-guided isolation and characterization of antibacterial compound from Aspergillus fumigatus HX-1 associated with Clam

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Xiaowen Xu ◽  
Siya Guo ◽  
Hui Chen ◽  
Zongyi Zhang ◽  
Xiangrong Li ◽  
...  
Keyword(s):  
Aspergillus Fumigatus ◽  
Isolation And Characterization ◽  
Antibacterial Compound

scholarly journals Isolasi dan Karakterisasi Aktinomisetes Penghasil Antibakteri Enteropatogen Escherichia coli K1.1, Pseudomonas pseudomallei 02 05, dan Listeria monocytogenes 5407

2007 ◽  
Vol 3 (1) ◽  
pp. 15 ◽  
Author(s):  
Dwi Ningsih Susilowati ◽  
Ratih Dewi Hastuti ◽  
Erny Yuniarti
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Acetate Solution ◽  
Antibacterial Compounds ◽  
Isolation And Characterization ◽  
Antibacterial Compound ◽  
Group A ◽  
Pseudomonas Pseudomallei

<p>Isolation and Characterization of Actinomycetes Producing<br />Antibacterial Compound into Enteropatogenik<br />Escherichia coli K1.1, Pseudomonas pseudomallei 02 05<br />and Listeria monocytogenes 5407. Dwi N. Susilowati,<br />Ratih D. Hastuti, and Erny Yuniarti. The resistance of<br />bacterial pathogens to some antibacterial agents and side<br />effects of the antibacterial usage demanded discovery of<br />new effective, safe, and active antibacterial compounds.<br />Some pathogenic bacteria, such as enteropathogen Escherichia<br />coli (EPEC) that cause diarrhoea on children and<br />infants, Pseudomonas pseudomallei that cause melioidosis<br />on human and animal, and Listeria monocytogenes that<br />cause listeriosis on newly born babies mortality and death of<br />pregnant woman. Actinomycetes is the largest bacterial<br />group that produce antibiotics. More than 10,000 antibacterial<br />compounds had been discovered, two-third of<br />them were produced by this bacterial group. A study was<br />done to isolate and characterize Actinomycetes producing<br />antibacterial compounds effective against EPEC K1.1 and P.<br />pseudomallei 02 05. Soil samples were taken from 39<br />locations in Indonesia and 115 actinomycetes isolates were<br />obtained. Two of the isolates, i.e., isolate A3.5 that was<br />effective against P. pseudomallei 02 05 and isolate F6.1 that<br />was effective against EPEC K1.1 evaluated further. The<br />isolate A3.5 had an optimum time 72 hours to produce antibacterial<br />compound, while F6.1 took 96 hours. The antibacterial<br />compounds produced by both isolates were dissolve<br />in the a 70% ethyl acetate solution, but not in a 40oC<br />warm methanol solution because it is very dissolved. The<br />antibacterial compound extracted from the isolate A3.5 had<br />a similar effectiveness to antibiotics bacithracyn 10 unit and<br />neomycin 30 g. On the other hand, the antibacterial<br />compound extracted from isolate F6.1 had a similar effectiveness<br />to antibiotics colistin 10 g and doxyciclin 30 g.<br />Further identification of the isolates suggested that both of<br />them belongs to the genera Streptomyces.</p>

scholarly journals Isolation and Characterization of Lytic Bacteriophages Infecting Acinetobacter baumannii

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ziad M.F. Al-Khozai ◽  
Mohammed N.H. Al-Kaabi
Keyword(s):  
Acinetobacter Baumannii ◽  
Burst Size ◽  
Sewage Water ◽  
Optimal Temperature ◽  
Narrow Host Range ◽  
Isolation And Characterization ◽  
Lysis Time ◽  
Wide Range ◽  
Complete Lysis

This study was carried out during the period from October 2015 to March 2016. It included the isolation of 42 isolates were identified asAcinetobacterbaumannii. All Isolates showed wide range of resistance to antibiotics. Two types of lytic bacteriophages primarily named Phage 1 and Phage 2 were isolated from sewage water. Phage 1 exhibited broad host spectrum it was lytic against all Acinetobacter baumannii isolates included for the study. Conversely,phage 2 have narrow host range as they could inhibit only 30 isolates,None of these phages inhibited a bacterium other than staphylococci species. The adsorption rate of Phage 1 was 3.1×10-10 ml min-1while Phage 2 was 1.7×10-10 ml min-1. Eclipse and Latent periods of Phage 1 were 6,17 minutes and for phage 2 were 7,14 minutes respectively,the burst size of Phage 1 and Phage 2 were 73±16 pfu\cell and 38±11 pfu\cell respectively. Complete lysis time of Phage 1 particles was 4.30 hours while the complete lysis time of Phage 2 particles was 6 hours. Phages passaging results showed a remarkable increment in PFU which were reached its maximal elevation at six passage in Phage 1 and fourth passage in Phage 2. Fitness of Phage 1 and Phage 2 were 24 ±5 pfu\cell and 26 ±4 pfu\cellrespectively through six generations. The Phage 1 and Phage 2 particles were stabled at a wide range of pH (6-10) and temperatures (30-50°C),the optimal temperature of two phages were 37°C.

Isolation and Characterization of Lytic Bacteriophages infecting Staphylococcus epidermidis

2016 ◽  
Vol 2 (1) ◽  
pp. 8-11
Author(s):  
Ream Saleem ◽  
Ghanim Aboud Jaber Al-Mola
Keyword(s):  
Staphylococcus Epidermidis ◽  
Plaque Assay ◽  
Sewage Water ◽  
Effect Of Temperature ◽  
Biochemical Tests ◽  
Phage Titer ◽  
Isolation And Characterization ◽  
Bacterial Lawn ◽  
Optimal Ph

The Staphylococcus epidermidis isolates were obtained during a period extended between September 2014 and January 2015, depending on biochemical tests and VITEK 2 system. Several sewage water samples were assayed using a plaque assay of double agar overlay as a source of S. Epidermidis phages. The bacteriophages were described depending on plaques size and shapes. Phage 1 was the most predominant in the bacterial lawn and able to infect other S. species such as S. aureus. Therefore, it was decided to study the effect of temperature on its original titer. The results revealed a gradual decrease in the phage titer with increasing dilution number. Each temperature at several incubation periods, significantly vary depending on phage titer. The optimum temperature was 40 ° C, while the 80 ° C was represented the inhibitor temperature. L.S.D. at level (0.05) for interaction was 39.552. The pH 6.5 – 7.5 were represented the optimal pH for the best phage activity while the phage titer beginning to decline in above and below this range of optimal pH , L.S.D. at level 0.05 was 17.898. In conclusion, our study found that Phage1 was considered as predominant phage because of their ability to infect other Staphylococci species such as S. Aureus.

scholarly journals Isolation and Characterization of Lytic Coliphages from Sewage Water

2017 ◽  
Vol 11 (1) ◽  
pp. 559-565 ◽  
Author(s):  
PU Megha ◽  
S Murugan ◽  
PS Harikumar
Keyword(s):  
Sewage Water ◽  
Isolation And Characterization
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