scholarly journals Incorporating pTGF-β1/calcium phosphate nanoparticles with fibronectin into 3-dimensional collagen/chitosan scaffolds: Efficient, sustained gene delivery to stem cells for chondrogenic differentiation

2012 ◽  
Vol 23 ◽  
pp. 81-93 ◽  
Author(s):  
X Cao ◽  
◽  
W Deng ◽  
Y Wei ◽  
Y Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Chondrogenic Differentiation ◽  
3 Dimensional ◽  
Calcium Phosphate Nanoparticles ◽  
Chitosan Scaffolds

Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis

2017 ◽  
Vol 5 (5) ◽  
pp. 972-981 ◽  
Author(s):  
Quazi T. H. Shubhra ◽  
Ayako Oyane ◽  
Hiroko Araki ◽  
Maki Nakamura ◽  
Hideo Tsurushima
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Process Optimization ◽  
Cell Transfection ◽  
Calcium Phosphate Nanoparticles ◽  
Infusion Fluids

The preparation of calcium phosphate nanoparticles from infusion fluids for gene delivery to stem cells and CHO-K1 cells is reported.

scholarly journals Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite-collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles

2016 ◽  
Vol 21 (7) ◽  
pp. 682-695 ◽  
Author(s):  
Taichi Tenkumo ◽  
Juan Ramón Vanegas Sáenz ◽  
Yukyo Takada ◽  
Masatoshi Takahashi ◽  
Olga Rotan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Collagen Scaffold ◽  
Calcium Phosphate Nanoparticles

PREPARATION OF CALCIUM PHOSPHATE NANOPARTICLES AS NON-VIRAL VECTORS FOR GENE DELIVERY SYSTEM

2017 ◽  
Vol 29 (04) ◽  
pp. 1750027 ◽  
Author(s):  
Ko-Chung Yen ◽  
I-Hua Chen ◽  
Feng-Huei Lin
Keyword(s):  
Gene Therapy ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Viral Vectors ◽  
Fluorescent Protein ◽  
Molar Ratio ◽  
Gene Delivery System ◽  
Calcium Phosphate Nanoparticles

A major aim of gene therapy is the efficient and specific delivery of therapeutic gene into the desired target tissues. Development of reliable vectors is a major challenge in gene therapy. The aim of this study is to develop calcium phosphate nanoparticles as novel non-viral vectors for the gene delivery system. Calcium phosphate nanoparticles were prepared by water-in-oil microemulsion method with a water to surfactant molar ratio, Wo [Formula: see text] 2–10. This paper studies the design and synthesis of ultra-low size, highly monodispersed DNA doped calcium phosphate nanoparticles of size around 100[Formula: see text]nm in diameter. The structure of DNA-calcium phosphate nanocomplex observed by TEM was displayed as a shell-like structure. This study used pEGFP as a reporter gene. The encapsulating efficiency to encapsulate DNA inside the nanoparticles was greater than 80%. In the MTT test, both calcium phosphate nanoparticles and DNA-calcium phosphate nanocomplex have no negative effect for 293T cells. By gel electrophoresis of free and entrapped pEGFP DNA, the DNA encapsulated inside the nanoparticles was protected from the external DNaseI environment. In vitro transfection studies in 293T cell-line, the DNA-calcium phosphate nanocomplex could be used safely to transfer the encapsulated DNA into the 293T cells and expression green fluorescent protein. The characteristic of DNA-calcium phosphate nanocomplex to deliver DNA belongs to slow release. The property of DNA-calcium phosphate nanocomplex was fit in the requirement of non-viral vectors for the gene delivery system.

scholarly journals Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

Nanoscale ◽  
2019 ◽  
Vol 11 (41) ◽  
pp. 19408-19421 ◽  
Author(s):  
Valentina Bordoni ◽  
Giacomo Reina ◽  
Marco Orecchioni ◽  
Giulia Furesi ◽  
Stefanie Thiele ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Calcium Phosphate Nanoparticles ◽  
Stimulation Of

Graphene oxide complexed with calcium phosphate nanoparticles enhances bone regeneration through signalling between monocytes and mesenchymal stem cells.

scholarly journals Directing Stem Cell Commitment by Amorphous Calcium Phosphate Nanoparticles Incorporated in PLGA: Relevance of the Free Calcium Ion Concentration

2020 ◽  
Vol 21 (7) ◽  
pp. 2627
Author(s):  
Olivier Gröninger ◽  
Samuel Hess ◽  
Dirk Mohn ◽  
Elia Schneider ◽  
Wendelin Stark ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Calcium Phosphate ◽  
Amorphous Calcium Phosphate ◽  
Marker Gene ◽  
Ion Concentration ◽  
Marker Genes ◽  
Calcium Phosphate Nanoparticles ◽  
Cell Commitment

The microenvironment of mesenchymal stem cells (MSCs) is responsible for the modulation in MSC commitment. Nanocomposites with an inorganic and an organic component have been investigated, and osteogenesis of MSCs has been attributed to inorganic phases such as calcium phosphate under several conditions. Here, electrospun meshes and two-dimensional films of poly(lactic-co-glycolic acid) (PLGA) or nanocomposites of PLGA and amorphous calcium phosphate nanoparticles (PLGA/aCaP) seeded with human adipose-derived stem cells (ASCs) were analyzed for the expression of selected marker genes. In a two-week in vitro experiment, osteogenic commitment was not found to be favored on PLGA/aCaP compared to pure PLGA. Analysis of the medium revealed a significant reduction of the Ca2+ concentration when incubated with PLGA/aCaP, caused by chemical precipitation of hydroxyapatite (HAp) on aCaP seeds of PLGA/aCaP. Upon offering a constant Ca2+ concentration, however, the previously observed anti-osteogenic effect was reversed: alkaline phosphatase, an early osteogenic marker gene, was upregulated on PLGA/aCaP compared to pristine PLGA. Hence, in addition to the cell–material interaction, the material–medium interaction was also important for the stem cell commitment here, affecting the cell–medium interaction. Complex in vitro models should therefore consider all factors, as coupled impacts might emerge.

Characterization of Calcium Phosphate Nanoparticles Based on a PEGylated Chelator for Gene Delivery

2017 ◽  
Vol 9 (12) ◽  
pp. 10435-10445 ◽  
Author(s):  
Xiangang Huang ◽  
Diana Andina ◽  
Jingshui Ge ◽  
Anne Labarre ◽  
Jean-Christophe Leroux ◽  
...  
Keyword(s):  
Calcium Phosphate ◽  
Gene Delivery ◽  
Calcium Phosphate Nanoparticles

scholarly journals Preparation of optimized lipid-coated calcium phosphate nanoparticles for enhanced in vitro gene delivery to breast cancer cells

2015 ◽  
Vol 3 (33) ◽  
pp. 6805-6812 ◽  
Author(s):  
Jie Tang ◽  
Li Li ◽  
Christopher B. Howard ◽  
Stephen M. Mahler ◽  
Leaf Huang ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Calcium Phosphate ◽  
Cell Growth ◽  
Gene Delivery ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cancer Cell Growth ◽  
Calcium Phosphate Nanoparticles ◽  
Transfection Agent

The optimized lipid coated calcium phosphate nanoparticles more efficiently deliver functional siRNA and inhibit the cancer cell growth, in comparison with the commercial transfection agent OligofactamineTM.

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