scholarly journals Gene transfection of human mesenchymal stem cells with a nano-hydroxyapatite-collagen scaffold containing DNA-functionalized calcium phosphate nanoparticles

2016 ◽  
Vol 21 (7) ◽  
pp. 682-695 ◽  
Author(s):  
Taichi Tenkumo ◽  
Juan Ramón Vanegas Sáenz ◽  
Yukyo Takada ◽  
Masatoshi Takahashi ◽  
Olga Rotan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Collagen Scaffold ◽  
Calcium Phosphate Nanoparticles

scholarly journals Enhanced Transfection of Human Mesenchymal Stem Cells Using a Hyaluronic Acid/Calcium Phosphate Hybrid Gene Delivery System

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 798 ◽  
Author(s):  
Jung Eun Lee ◽  
Yue Yin ◽  
Su Yeon Lim ◽  
E. Seul Kim ◽  
Jaeback Jung ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Calcium Phosphate ◽  
Gene Delivery ◽  
Delivery System ◽  
Gene Transfection ◽  
Human Mesenchymal Stem Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Gene Delivery System

Human mesenchymal stem cells (hMSCs) show enormous potential in regenerative medicine and tissue engineering. However, current use of hMSCs in clinics is still limited because there is no appropriate way to control their behavior in vivo, such as differentiation to a desired cell type. Genetic modification may provide an opportunity to control the cells in an active manner. One of the major hurdles for genetic manipulation of hMSCs is the lack of an efficient and safe gene delivery system. Herein, biocompatible calcium phosphate (CaP)-based nanoparticles stabilized with a catechol-derivatized hyaluronic acid (dopa-HA) conjugate were used as a carrier for gene transfection to hMSCs for improved differentiation. Owing to the specific interactions between HA and CD44 of bone marrow-derived hMSCs, dopa-HA/CaP showed significantly higher transfection in hMSCs than branched polyethylenimine (bPEI, MW 25 kDa) with no cytotoxicity. The co-delivery of a plasmid DNA encoding bone morphogenetic protein 2 (BMP-2 pDNA) and micro RNA 148b (miRNA-148b) by dopa-HA/CaP achieved significantly improved osteogenic differentiation of hMSCs.

scholarly journals Synergistic acceleration in the osteogenesis of human mesenchymal stem cells by graphene oxide–calcium phosphate nanocomposites

2014 ◽  
Vol 50 (62) ◽  
pp. 8484-8487 ◽  
Author(s):  
Rameshwar Tatavarty ◽  
Hao Ding ◽  
Guijin Lu ◽  
Robert J. Taylor ◽  
Xiaohong Bi
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Calcium Phosphate ◽  
Synergistic Effect ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation ◽  
Calcium Phosphate Nanoparticles

Nanocomposites consisting of oblong ultrathin plate shaped calcium phosphate nanoparticles and graphene oxide microflakes were synthesized and have demonstrated markedly synergistic effect in accelerating stem cell differentiation to osteoblasts.

Preparation of dexamethasone-loaded calcium phosphate nanoparticles for the osteogenic differentiation of human mesenchymal stem cells

2017 ◽  
Vol 5 (33) ◽  
pp. 6801-6810 ◽  
Author(s):  
Ying Chen ◽  
Jingchao Li ◽  
Naoki Kawazoe ◽  
Guoping Chen
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Biphasic Calcium Phosphate ◽  
Human Mesenchymal Stem Cells ◽  
Calcium Phosphate Nanoparticles

Dexamethasone (DEX)-loaded biphasic calcium phosphate nanoparticles (BCP-NPs) are prepared by incorporation of DEX during or after the formation of BCP-NPs. The DEX-loaded BCP-NPs release DEX in a sustained manner and enhance the osteogenic differentiation of hMSCs.

Calcium phosphate-based particles influence osteogenic maturation of human mesenchymal stem cells

2009 ◽  
Vol 5 (4) ◽  
pp. 1294-1305 ◽  
Author(s):  
L. Saldaña ◽  
S. Sánchez-Salcedo ◽  
I. Izquierdo-Barba ◽  
F. Bensiamar ◽  
L. Munuera ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Human Mesenchymal Stem Cells

Two-layer membranes of calcium phosphate/collagen/PLGA nanofibres: in vitro biomineralisation and osteogenic differentiation of human mesenchymal stem cells

Nanoscale ◽  
2011 ◽  
Vol 3 (2) ◽  
pp. 401-409 ◽  
Author(s):  
Nora Hild ◽  
Oliver D. Schneider ◽  
Dirk Mohn ◽  
Norman A. Luechinger ◽  
Fabian M. Koehler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Calcium Phosphate ◽  
Osteogenic Differentiation ◽  
Human Mesenchymal Stem Cells

scholarly journals Stimulation of bone formation by monocyte-activator functionalized graphene oxide in vivo

Nanoscale ◽  
2019 ◽  
Vol 11 (41) ◽  
pp. 19408-19421 ◽  
Author(s):  
Valentina Bordoni ◽  
Giacomo Reina ◽  
Marco Orecchioni ◽  
Giulia Furesi ◽  
Stefanie Thiele ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Calcium Phosphate ◽  
Bone Formation ◽  
Functionalized Graphene ◽  
Functionalized Graphene Oxide ◽  
Calcium Phosphate Nanoparticles ◽  
Stimulation Of

Graphene oxide complexed with calcium phosphate nanoparticles enhances bone regeneration through signalling between monocytes and mesenchymal stem cells.

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