scholarly journals The Effect of Modified Atmospheric Packaging on Beef Color Sensory Characteristics of Different Muscle pH Categories

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
A. Cassens ◽  
G. Mafi ◽  
D. VanOverbeke ◽  
R. Ramanathan

ObjectivesAny deviation from the bright-red color of beef can lead to discounted price or consumer rejection. Fresh beef lean color is influenced by pH. Various packaging techniques have been developed to enhance the lean color of beef steaks. Therefore, the objective of the current study was to evaluate the effects of modified atmospheric packaging on three different beef muscle pH categories.Materials and MethodsThe three categories evaluated for this study were: Normal (pH = 5.57 ± 0.1; N-pH), Moderately high (pH = 5.70 ± 0.09; M-pH), and High (pH = 6.39 ± 0.03; H-pH). The pH was taken on the carcass, at the 12th and 13th rib interface within 72 h of harvest at a commercial beef processing plant. Strip loins were fabricated from each carcass (n = 12) and sent to Oklahoma State University for further analysis. Strip loins were then cut into 2.54 cm steaks and randomly assigned to 1 of 3 packaging treatments: polyvinyl chloride overwrap (PVC), carbon monoxide modified atmosphere packaging (CO-MAP; 0.4% CO, 69.6% N, and 30% CO2) and high-oxygen modified atmospheric packaging (HiOx-MAP; 80% O2, and 20% CO2). Visual color measurements for muscle color (MC; (1 = extremely bright cherry-red and 7 = extremely dark red), and surface discoloration (SD; 1 = no discoloration [0%] and 7 = extensive discoloration [81–100%]) were recorded on d 2, 4, and 6 of retail display by a trained panel. Data were analyzed using the Mixed Procedure of SAS.ResultsFor all pH treatments, PVC packaging possessed the darkest muscle color (P < 0.05) score compared to CO- and HiOx-MAP. When comparing N-pH, M-pH, and H-pH values, CO-MAP had approximately a 27.3%, 22.2%, and 25.3% improvement in muscle color, indicating a brighter lean color compared to PVC. Additionally, HiOx-MAP had approximately a 10.9%, 17.4%, and 16.5% improvement in muscle color score for N-pH, M-pH, and H-pH, respectively. When packaging steaks in either CO- or HiOx-MAP there was no significant difference (P > 0.05) between d 4 and 6 of retail display for muscle color. However, there was a significant (P < 0.05) darkening in muscle color for steaks packaged in PVC from d 4 to 6 of retail display. By the fourth d of retail, N-pH steaks packaged in PVC had 38.3% and 39.0% greater (P < 0.05) surface discoloration than CO- and HiOx-MAP, respectively.ConclusionThese results suggest that packaging steaks of different pH categories in CO- or HiOx-MAP can improve the surface color compared to PVC packaging.

2019 ◽  
Vol 3 (2) ◽  
Author(s):  
L. Yoder ◽  
A. Cassens ◽  
G. Mafi ◽  
D. VanOverbeke ◽  
R. Ramanathan

ObjectivesDark-cutting carcasses occur when muscle pH does not decrease sufficiently during rigor mortis because of antemortem glycogen depletion. Typical dark-cutting beef have a pH > 6.0, however, atypical dark-cutting (ADC) beef have a pH around 5.7- 5.9 but have a darker lean color similar to dark-cutting beef. Previous studies noted that greater muscle pH in dark-cutting beef increase oxygen consumption and decrease myoglobin oxygenation. However, limited research has determined the biochemical basis of ADC. Therefore, the objectives of this study were to characterize the biochemical basis of ADC beef carcasses and to utilize modified atmosphere packaging (MAP) and rosemary enhancement to improve appearance during retail display.Materials and MethodsStrip loins from ADC and USDA Low Choice (C) carcasses were selected from a commercial beef processing plant and transported to the Food and Agricultural Products Center in Stillwater, OK. Control (C) and ADC steaks (prior to enhancement and packaging) were utilized to measure pH, color, oxygen consumption, and proximate compositions. Loin sections were cut into halves and randomly assigned to combinations of packaging types and enhancement treatment. Packaging included high-oxygen MAP (HiOx-MAP; 80% oxygen and 20% carbon dioxide), carbon monoxide MAP (CO-MAP; 0.4% CO, 69.6% N, and 30% CO2), and PVC. Enhancement includes 0.1% rosemary oleoresin pumped to 10% of loin green weights. Following enhancement and packaging, steaks were displayed under retail conditions for 6 d to measure color changes using a HunterLab spectrophotometer. The data were analyzed using the Mixed Procedure of SAS, and the experiment was replicated 13 times (n = 13).ResultsThere were no differences for initial pH or proximate compositions (P < 0.05) between C and ADC. However, ADC had lower initial lightness (L* values), redness (a* values), and red intensity (chroma) compared with C. Further, ADC had greater (P < 0.05) oxygen consumption than C. There was a significant packaging × enhancement × display time interaction resulted for L*, a*, and chroma. CO-MAP and HiOx-MAP in combination with rosemary enhancement improved (P < 0.05) redness of ADC by 61.7% and 42.3%, respectively, compared with ADC in PVC packaging. Similarly, MAP and enhancement improved lightness (L* values) and chroma compared with ADC in PVC. By Day 6 of display, enhanced ADC steaks packaged in both HiOx-MAP and CO-MAP had similar color parameters to control choice steaks; however, non-enhanced ADC steaks had significantly lower a* (P < 0.0001) and chroma (P < 0.01) values when packaged in HiOx-MAP when compared to both C and enhanced ADC.ConclusionThe results suggest that ADC beef has greater oxygen consumption than C steaks even at similar muscle pH. Use of modified atmospheric packaging in combination with enhancement has the potential to improve surface color of ADC beef.


2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 25-26
Author(s):  
Andrew M Cassens ◽  
Gretchen Mafi ◽  
Deb VanOverbeke ◽  
Ranjith Ramanathan

Abstract Any deviation from the bright-red color of beef leads to discounted price or consumer rejection. Atypical dark-cutting beef represents darker color of lean around a pH 5.6 – 5.8. However, limited studies have determined the postharvest practices to improve the lean color of atypical dark-cutting beef. Therefore, the objective was to evaluate the effects of rosemary-enhancement and modified atmospheric packaging on atypical dark-cutting beef. Atypical dark-cutting beef strip loins (n = 13, pH=5.70 ± 0.09; ADC) and USDA Choice beef strip loins (n = 6, pH=5.57 ± 0.1; CH) were selected within 72 h of harvest. Atypical dark-cutting strip loins were divided into 2 sections, and randomly assigned to one of the following enhancement treatments: control non-enhanced (ADC), or a 1.1% rosemary-enhanced treatment (ADCE). After enhancement, 2.54 cm steaks were cut from CH, ADC, and ADCE strip loins and assigned to 1 of 3 packaging treatments: polyvinyl chloride overwrap (PVC), carbon monoxide modified atmosphere packaging (CO-MAP; 0.4% CO, 69.6% N, and 30% CO2), and high-oxygen modified atmospheric packaging (HiOx-MAP; 80% O2 and 20% CO2). A HunterLab MiniScan was used to determine color during the 6 d simulated retail display. Visual color measurement for muscle darkening (MD), muscle color (MC), and surface discoloration (SD) were recorded on d 2, 4, and 6 of retail display. There was a significant enhancement x packaging interaction for instrumental and visual color measurements. For all packaging types, ADCE steaks possessed greater L* values than DC steaks. In addition, ADCE steaks packaged in HiOx-MAP or CO-MAP had brighter-red color than ADC steaks. Furthermore, ADCE and C steaks had less muscle darkening compared with ADC steaks for MD and MC attributes. The results suggest that enhancement and modified atmospheric packaging have the potential to improve the surface color of atypical dark-cutting beef.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
M. Perry ◽  
M. Pfeiffer ◽  
D. VanOverbeke ◽  
R. Ramanathan ◽  
G. Mafi

ObjectivesMeat color is extremely influential in purchasing decisions as consumers associate a bright-red color with freshness. The type of finishing diet can influence beef color. Previous studies have shown that grass-finished cattle have darker muscle color than grain-finished cattle. With the use of modified atmospheric packaging (MAP), beef purveyors are able to vary the gas compositions within a package and enhance beef color. However, limited studies have determined the effects of modified atmospheric packaging on grass-finished beef color. The objective of this study was to determine how finishing diet and packaging type affects the color of the longissimus dorsi (LD) muscle.Materials and MethodsDuring the stocker period, all of the cattle were on a forage diet. Cattle were then randomly assigned to either a conventional grain-based diet or an alfalfa pasture diet for finishing. Both conventionally and pasture-finished cattle were fed for 91 d. Cattle were slaughtered on the same day at a commercial beef processing facility under normal conditions and chilled for approximately 30 h. After grading, one strip loin from each carcass was collected and transported to Oklahoma State University. At 11 d postmortem, one steak (n = 60) from each strip loin was vacuum packaged and randomly assigned to display. Those steaks were then randomly assigned to PVC, HiOx-MAP (80% oxygen and 20% carbon dioxide), or CO-MAP (0.4% carbon monoxide, 69.5% nitrogen, and 30% carbon dioxide) packaging and were displayed under retail conditions for 5 d. Muscle darkening (MD), muscle color (MC), and surface discoloration (SD) were all analyzed by a trained panel (n = 6). MD was evaluated only on d 0 and MC and SD were scored once every 24 h for 0, 1, 2, 3, and 4 d. Lipid oxidation was measured by thiobarbituric acid reactive substances (TBARS) assay on d 4. Data were analyzed using the Mixed Procedure of SAS.ResultsThere was a significant display day by finishing diet by packaging interaction (P < 0.05) for muscle color and surface discoloration. There was also a significant finishing diet by packaging interaction (P < 0.05) for muscle darkening. Steaks packaged in HiOx-MAP remained the most stable in color and the brightest cherry-red colored throughout display time (P < 0.05) compared with other packaging types. PVC was the most discolored (P < 0.05) on d 3 and 4 when compared to HiOx-MAP and CO-MAP with the grain-finished PVC packaged steaks showing the most discoloration on d 4. Pasture-finished steaks packaged in CO-MAP displayed the darkest colored muscle (P < 0.05) on d 0. Steaks packaged in PVC had a higher amount of lipid oxidation (P < 0.05) compared with other packaging types.ConclusionThese results indicate that HiOx-MAP more effectively maintains the desired beef color of bright cherry-red for pasture-finished beef. The results also indicate that the use of appropriate packaging type can minimize the losses due to discoloration of steaks from either grain or grass-finished beef.


1980 ◽  
Vol 43 (11) ◽  
pp. 842-849 ◽  
Author(s):  
G. C. SMITH ◽  
L. C. HALL ◽  
C. VANDERZANT

Biceps femoris steaks (n = 432) were inoculated with each of four Lactobacillus sp. (atypical streptobacteria and betabacteria) at a high or low level of cell concentration, vacuum-packaged, stored for up to 35 days at 1–3 C, displayed under retail conditions for 3 days and subsequently cooked for sensory panel analysis. Although there was some evidence that use of high level, rather than low level or no, inoculation with Lactobacillus cultures resulted in the lowest muscle surface pH and the brightest muscle color, the least surface discoloration and the highest overall appearance ratings following storage for 0–35 days, there were negative effects which negated efficacy of high level inoculation. Negative effects of use of high level, rather than low level or no, inoculation with Lactobacillus cultures included the highest incidence of off-odor, the highest incidence of defects during retail display (most extensive surface discoloration, most extensive peripheral discoloration, least desirable overall appearance) and the lowest palatability (juiciness, tenderness, flavor and overall palatability) ratings. Few of the differences among steaks were statistically significant but the preponderance of evidence (largely that based on numerical differences) suggests that inoculation with the Lactobacillus cultures used in this study will not improve storage life, retail caselife or palatability of beef.


2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 22-22
Author(s):  
Andrew M Cassens ◽  
Gretchen G Mafi ◽  
Deb L VanOverbeke ◽  
Ranjith Ramanathan

Abstract Quality variation within the pork industry is commonly associated with intramuscular fat and muscle color. The objective of this study was to determine the effects of packaging, pork quality types, and cooking temperature on color and shear force. Normal, non-enhanced (N, n = 10), Enhanced with salt and phosphate (E, n = 10) and Highly Marbled (H, n = 10) pork loins from a local food distributor. Loins were cut into 2.54-cm-thick chops and assigned to retail display or tenderness evaluation. Retail display chops were assigned to 1 of 3 packaging treatments: polyvinyl chloride overwrap (PVC), carbon monoxide modified atmosphere packaging (CO-MAP) and high-oxygen modified atmospheric packaging (HiOx-MAP). Visual color measurement for muscle color (MC), fat color (FC), surface discoloration (SD), and surface color uniformity (SCU) was recorded on d 0, 2, and 4 of retail display. Pork tenderness was evaluated at 3 different degrees of doneness (63, 68, & 74°C) using Warner-Bratzler shear (WBS) force. There were significant treatment and packaging interactions for muscle color. For muscle color score, E pork loins had a significantly lower (P &lt; 0.05) muscle color score compared with N and H loins indicative of a brighter, more pale pink color. In addition, CO- and HiOx-MAP packaged chops were significantly brighter (P &lt; 0.05) compared to PVC chops. There were treatment and degree of doneness main effects (P &lt; 0.05) for WBS force values. Enhanced pork loins were more tender (P &lt; 0.05) than N pork loins. Furthermore, pork chops cooked to 63 °C were more tender than chops cooked to 68 and 73°C. In conclusion, enhanced chops packaged in CO- or HiOx-MAP result in a brighter color of lean compared non-enhanced and high-quality. Additionally, the results suggest that enhanced, non-enhanced, and high-quality pork products available in market have different quality parameters.


Author(s):  
Md. Azizul Haque ◽  
Md. Asaduzzaman ◽  
Md. Sultan Mahomud ◽  
Md. Rizvi Alam ◽  
Alin Khaliduzzaman ◽  
...  

AbstractFresh-cut lettuce is a very well-known salad for today's routines because it obliges minimal preparation to minimize the loss of health beneficial vitamins, minerals, antioxidants and other phytochemicals. It is a prodigious challenge to serve its consumers fresh. Quality of freshly processed lettuce under high CO2 modified atmosphere packaging (MAP) has been investigated as a realistic alternative technique for its preservation. Storage under high CO2 atmospheric treatments exhibited a significant impact in microbial development, electrolyte leakage, volatile metabolites and sensory quality of fresh-cut iceberg lettuce. This storage condition (MAP 1: 5 kPa O2 and 20 kPa CO2 balanced by N2 at 7 °C for 6 days) inhibited the growth of mesophilic bacteria and yeasts; delayed the enzymatic browning (cut-edges and intact surface) of fresh-cut iceberg lettuce and overall visual quality was also in acceptance limit. The development of off-odors was perceived in high CO2 MAP as a consequence of volatiles (ethanol and acetaldehyde) accumulation which was persisted at an inexcusable level during 6 days of storage periods.


2014 ◽  
Vol 81 (2) ◽  
pp. 713-725 ◽  
Author(s):  
John W. Schmidt ◽  
Getahun E. Agga ◽  
Joseph M. Bosilevac ◽  
Dayna M. Brichta-Harhay ◽  
Steven D. Shackelford ◽  
...  

ABSTRACTSpecific concerns have been raised that third-generation cephalosporin-resistant (3GCr)Escherichia coli, trimethoprim-sulfamethoxazole-resistant (COTr)E. coli, 3GCrSalmonella enterica, and nalidixic acid-resistant (NALr)S. entericamay be present in cattle production environments, persist through beef processing, and contaminate final products. The prevalences and concentrations of these organisms were determined in feces and hides (at feedlot and processing plant), pre-evisceration carcasses, and final carcasses from three lots of fed cattle (n= 184). The prevalences and concentrations were further determined for strip loins from 103 of the carcasses. 3GCrSalmonellawas detected on 7.6% of hides during processing and was not detected on the final carcasses or strip loins. NALrS. entericawas detected on only one hide. 3GCrE. coliand COTrE. coliwere detected on 100.0% of hides during processing. Concentrations of 3GCrE. coliand COTrE. colion hides were correlated with pre-evisceration carcass contamination. 3GCrE. coliand COTrE. coliwere each detected on only 0.5% of final carcasses and were not detected on strip loins. Five hundred and 42 isolates were screened for extraintestinal pathogenicE. coli(ExPEC) virulence-associated markers. Only two COTrE. coliisolates from hides were ExPEC, indicating that fed cattle products are not a significant source of ExPEC causing human urinary tract infections. The very low prevalences of these organisms on final carcasses and their absence on strip loins demonstrate that current sanitary dressing procedures and processing interventions are effective against antimicrobial-resistant bacteria.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
C. Edmundson ◽  
A. Cassens ◽  
G. Mafi ◽  
D. Stein ◽  
A. Riggs ◽  
...  

ObjectivesMeat color is often seen by consumers as an indicator of freshness and wholesomeness. Nitrite-embedded (NE) packaging forms nitric oxide myoglobin, which imparts a bright red color similar to oxymyoglobin. However, limited research has determined the effects of NE packaging to improve the appearance of atypically dark cutting beef. Consumers’ perception of NE packaging ultimately determines its success in the market. Educating consumers through infographics can transfer knowledge more effectively than text alone, potentially being a useful method to introduce and simplify the complexity of NE packaging’s role in improving the surface color of beef steaks. The objectives of this study were to evaluate FreshCase® nitrite-embedded packaging’s effect on atypical dark-cutting beef steaks and to evaluate student consumer perception of nitrite-embedded packaging improving the surface color of beef before and after exposure to infographics containing equal content.Materials and MethodsAtypical dark-cutting (n = 13, pH 5.70 ± 0.09) and normal-pH (n = 13, pH = 5.57 ± 0.1) USDA Low Choice beef strip loins were selected 3 d postharvest. Atypical dark-cutting loins were cut into 2.54 cm thick steaks and randomly packaged in polyvinyl chloride film (PVC) or NE film. Normal-pH control loins were cut 2.54 cm and randomly packaged in PVC overwrap. Packages were placed in a coffin-style retail case under fluorescent lighting for 6 d. Instrumental color was observed every 24 h using a HunterLab MiniScan XE spectrophotometer. The color was determined as a* values and chroma. In the second objective, surveys using a ten-point Likert sliding scale (0 = not familiar at all, 10 = extremely familiar) were randomly allocated and emailed via Qualtrics to students enrolled in the Introduction to Animal Science course at Oklahoma State University. These surveys used a pre-questionnaire to evaluate students’ pre-perception of their knowledge of beef color and NE packaging. After the pre-perception questionnaire students were provided one of the following: a static infographic presented as a still image with annotated graphics, a 46 s video infographic with audio and animated graphics, or both infographic formats. A post-questionnaire followed exposure to students’ respective infographic to evaluate changes in the perception of knowledge.ResultsAtypical dark-cutting steaks treated in NE packaging had higher (P < 0.05, more red intensity) chroma and a* values compared to atypical dark-cutting steaks in PVC on d 4, 5, and 6. There was a significant difference (P < 0.05) in the students’ (n = 288) pre- and post-questionnaire self-assessment of their familiarity with NE packaging. Prior to randomly viewing infographics, students were less familiar (= 3.18) with NE packaging than after viewing infographics (= 6.46). However, there was no significant difference in perceptions (P = 0.22) between viewing the different infographic formats.ConclusionThe results suggest that NE packaging with consumer education can improve their perceptions and knowledge and enhance the appearance of atypical dark-cutting beef.


2019 ◽  
Vol 3 (2) ◽  
Author(s):  
S. Huang ◽  
B. M. Bohrer

ObjectivesNovel, non-allergenic ingredients with properties that improve the quality of processed meat products are needed for the meat industry. The objectives of this study were to investigate the effect of breadfruit (Artocarpus altilis) flour on color of comminuted beef compared with other flour sources.Materials and MethodsFlour sources included breadfruit, corn, soy, tapioca, and wheat. All flours were obtained commercially and were unmodified. Lean beef (from the same commercially sourced batch targeted to 90% lean and 10% fat), 10% ice, 1.5% salt, and flour sources at two inclusion levels (2.5% and 5%) were mixed using a bowl chopper to prepare beef patties for evaluation. The ground beef was manufactured into 115 g patties that were placed on a retail display shelf under continuous LED lighting at 4°C for 7 d. Lighting was measured periodically during the study and LUX was ensured to be between 1612.5 lux and 2152.0 lux. Objective CIE L* (lightness), a* (redness), and b* (yellowness), chroma, and hue scores were collected with a Minolta CR-400 Chroma meter (Konica Minolta Sensing, Inc., Osaka, Japan) utilizing a D65 light source and a 0° observer with an aperture size of 8 mm on each day of the simulated retail display. This study was conducted in three independent replicates for each treatment. Statistical analyses for parameters (L*, a*, b*, chroma, and hue) were conducted using the MIXED procedure of SAS with fixed effects of flour source*inclusion level, day, and their interaction. Least square means were separated using the PDIFF option with a Tukey-Kramer adjustment. Differences were considered statistically different at P < 0.05.ResultsThe interaction of storage day and treatment significantly (P ≤ 0.001) affected a*, b*, chroma, and hue. There was not an interaction of storage day and treatment for L*. Both the main effect of storage day and the main effect of treatment significantly (P < 0.01) affected all the attributes measured in this study. Mean L* over the display period of beef patties prepared with 2.5% breadfruit flour were not different (P = 0.95) compared with control samples. There was no significant difference between the mean hue over the 7-d display period of the beef patties prepared with 2.5% breadfruit flour compared with control samples. a* decreased at different rates for each treatment throughout the display period. Beef patties prepared with 2.5% and 5% breadfruit flour were redder (greater a*; (P < 0.05)) compared with other treatments and control samples over the 7-d display period. To the contrary, a* values of beef patties prepared with soy flour were less than (P < 0.05) other treatments and the control samples on Day 0 and Day 1 and remained constant at lower values as the display period increased.ConclusionBreadfruit flour improved the redness of comminuted beef products immediately and prevented discoloration for a longer period. The results indicate that breadfruit flour can effectively improve initial color and stability of color in processed beef products. More research is warranted to further investigate the mechanism of action of breadfruit flour in governing the color properties of comminuted beef products.


2005 ◽  
Vol 45 (5) ◽  
pp. 585 ◽  
Author(s):  
H. A. Channon ◽  
S. R. Baud ◽  
P. J. Walker

This study was conducted to determine the effect of electrical stimulation [control or stimulated (applied for 20 s using a constant current of 600 mA peak and maximum voltage of 550 V with a frequency of either 14 Hz or 10 Hz], ageing period (7, 21 or 35 days) and packaging method [modified atmosphere packaging (MAP) or overwrapped] on the retail display life of lamb [bone-in loin (M. longissimus thoracis et lumborum) chops and boneless leg steaks from the knuckle (M. quadriceps femoris)]. The colour and retail acceptability of lamb cuts were assessed both subjectively and objectively, using a chromameter, after 3 (midpoint of commercial retail display life; midpoint) and 5 (endpoint of commercial retail display life; endpoint) days for overwrapped product and at 5 (midpoint) and 9 (endpoint) days for MAP product. Retail display life of lamb (assessed both objectively and subjectively) was improved (P<0.001) by packaging lamb cuts into modified atmosphere compared with overwrapped lamb cuts. Electrical stimulation and length of ageing period before preparation into retail cuts did not influence colour or retail display life of lamb loin chops or leg steaks. Although the interaction of ageing period and packaging method were significant (P<0.001) for subjective colour, acceptability and retail grade of lamb loin chops and leg steaks at the midpoint of display life, these effects were inconsistent. This study highlighted that further work is necessary to determine potential means of improving the colour stability of lamb to enhance its retail display life, particularly cuts of the M. quadriceps femoris.


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