Nanosized, peptide-based multicomponent DNA delivery systems: optimization of endosome escape activity

Nanomedicine ◽  
2016 ◽  
Vol 11 (8) ◽  
pp. 907-919 ◽  
Author(s):  
Yu Wan ◽  
Peter M Moyle ◽  
Michelle P Christie ◽  
Istvan Toth
Keyword(s):  
Dna Delivery ◽  
Delivery Systems ◽  
Endosome Escape

scholarly journals Nanovesicle-Mediated Delivery Systems for CRISPR/Cas Genome Editing

Pharmaceutics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1233
Author(s):  
Dongyoon Kim ◽  
Quoc-Viet Le ◽  
Yina Wu ◽  
Jinwon Park ◽  
Yu-Kyoung Oh
Keyword(s):  
Genome Editing ◽  
Delivery Systems ◽  
Research Trends ◽  
Great Promise ◽  
Therapeutic Modalities ◽  
Guide Rnas ◽  
Endosome Escape ◽  
Nonviral Delivery ◽  
Potential Tool ◽  
Delivery Technology

Genome-editing technology has emerged as a potential tool for treating incurable diseases for which few therapeutic modalities are available. In particular, discovery of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas system together with the design of single-guide RNAs (sgRNAs) has sparked medical applications of genome editing. Despite the great promise of the CRISPR/Cas system, its clinical application is limited, in large part, by the lack of adequate delivery technology. To overcome this limitation, researchers have investigated various systems, including viral and nonviral vectors, for delivery of CRISPR/Cas and sgRNA into cells. Among nonviral delivery systems that have been studied are nanovesicles based on lipids, polymers, peptides, and extracellular vesicles. These nanovesicles have been designed to increase the delivery of CRISPR/Cas and sgRNA through endosome escape or using various stimuli such as light, pH, and environmental features. This review covers the latest research trends in nonviral, nanovesicle-based delivery systems that are being applied to genome-editing technology and suggests directions for future progress.

scholarly journals A self-assembling amphiphilic peptide nanoparticle for the efficient entrapment of DNA cargoes up to 100 nucleotides in length

Soft Matter ◽  
2020 ◽  
Vol 16 (6) ◽  
pp. 1678-1691 ◽  
Author(s):  
Shabnam Tarvirdipour ◽  
Cora-Ann Schoenenberger ◽  
Yaakov Benenson ◽  
Cornelia G. Palivan
Keyword(s):  
Dna Delivery ◽  
Delivery Systems ◽  
Viral Dna ◽  
Self Assembling ◽  
Double Stranded Dna ◽  
Amphiphilic Peptide ◽  
Low Efficiency

To overcome the low efficiency and cytotoxicity associated with most non-viral DNA delivery systems we developed a purely peptidic self-assembling system that is able to entrap single- and double-stranded DNA of up to 100 nucleotides in length.

Lactococcus lactis as a live vector: Heterologous protein production and DNA delivery systems

2011 ◽  
Vol 79 (2) ◽  
pp. 165-175 ◽  
Author(s):  
Daniela Santos Pontes ◽  
Marcela Santiago Pacheco de Azevedo ◽  
Jean-Marc Chatel ◽  
Philippe Langella ◽  
Vasco Azevedo ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Protein Production ◽  
Heterologous Protein ◽  
Dna Delivery ◽  
Delivery Systems ◽  
Heterologous Protein Production

NANOMEDICINES: DELIVERING DRUGS USING BOTTOM UP NANOTECHNOLOGY

2005 ◽  
Vol 04 (05n06) ◽  
pp. 855-861 ◽  
Author(s):  
MARTIN GARNETT
Keyword(s):  
Drug Delivery ◽  
Drug Release ◽  
Biodegradable Polymers ◽  
Drug Loading ◽  
Dna Delivery ◽  
Delivery Systems ◽  
The Body ◽  
Nanosized Materials

The use of nanosized materials changes the way in which drugs are handled by the body and offers opportunities to improve drug delivery. The physiological mechanisms controlling the distribution of nanosized materials (enhanced permeability and retention effect, cellular uptake pathways and opsonisation/elimination of nanoparticles) are described. Two different nanosized drug delivery systems are considered; drug delivery and DNA delivery. The deficiencies of currently available biodegradable polymers for preparation of drug containing nanoparticles are mainly the amount of drug that can be incorporated and the rapid rate of drug release. The development of new biodegradable polymers which can interact with the drug and so significantly increase drug loading and decrease the rate of drug release are outlined. DNA delivery necessitates overcoming a variety of biological barriers. We are developing polyelectrolyte complexes of DNA with cationic polyamidoamines (PAA) as a delivery system. Complexing PAA with DNA results in good transfection of cells in vitro. However, in vivo, a more complex arrangement of PAA, Polyethylene glycol-PAA copolymers, DNA and the use of ligands will be required. Despite these efforts, further developments will be needed in nanotechnology for both drug and DNA nanoparticle delivery systems to achieve our clinical objectives.

scholarly journals Counter-ion effect on surfactant–DNA gel particles as controlled DNA delivery systems

Soft Matter ◽  
2012 ◽  
Vol 8 (11) ◽  
pp. 3200 ◽  
Author(s):  
M. Carmen Morán ◽  
Tania Alonso ◽  
Filipe S. Lima ◽  
M. Pilar Vinardell ◽  
M. Graça Miguel ◽  
...  
Keyword(s):  
Dna Delivery ◽  
Delivery Systems ◽  
Counter Ion ◽  
Gel Particles

Development and Characterization of New Cyclodextrin Polymer-Based DNA Delivery Systems

2008 ◽  
Vol 19 (12) ◽  
pp. 2311-2320 ◽  
Author(s):  
Virginie Burckbuchler ◽  
Véronique Wintgens ◽  
Christian Leborgne ◽  
Sophie Lecomte ◽  
Nadine Leygue ◽  
...  
Keyword(s):  
Dna Delivery ◽  
Delivery Systems ◽  
Cyclodextrin Polymer

Structural characterization of novel gemini non-viral DNA delivery systems for cutaneous gene therapy

2006 ◽  
Vol 1 (2) ◽  
pp. 165-176 ◽  
Author(s):  
Marianna Foldvari ◽  
Ildiko Badea ◽  
Shawn Wettig ◽  
Ronald Verrall ◽  
Mukasa Bagonluri
Keyword(s):  
Gene Therapy ◽  
Structural Characterization ◽  
Dna Delivery ◽  
Delivery Systems ◽  
Viral Dna

Transforming the plastome: genetic markers and DNA delivery systems

Euphytica ◽  
1995 ◽  
Vol 85 (1-3) ◽  
pp. 29-34 ◽  
Author(s):  
P. J. Dix ◽  
T. A. Kavanagh
Keyword(s):  
Genetic Markers ◽  
Dna Delivery ◽  
Delivery Systems
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