Rubella virus capsid protein: a small protein with big functions

2010 ◽  
Vol 5 (4) ◽  
pp. 571-584 ◽  
Author(s):  
Carolina S Ilkow ◽  
Steven D Willows ◽  
Tom C Hobman
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Protein A ◽  
Virus Capsid ◽  
Small Protein ◽  
Virus Capsid Protein

scholarly journals Analysis of Rubella Virus Capsid Protein-Mediated Enhancement of Replicon Replication and Mutant Rescue

2006 ◽  
Vol 80 (8) ◽  
pp. 3966-3974 ◽  
Author(s):  
Wen-Pin Tzeng ◽  
Jason D. Matthews ◽  
Teryl K. Frey
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Fluorescent Protein ◽  
Vero Cells ◽  
Basic Step ◽  
Virus Capsid ◽  
Replicase Protein ◽  
Virus C ◽  
Green Fluorescent ◽  
Virus Capsid Protein

ABSTRACT The rubella virus capsid protein (C) has been shown to complement a lethal deletion (termed ΔNotI) in P150 replicase protein. To investigate this phenomenon, we generated two lines of Vero cells that stably expressed either C (C-Vero cells) or C lacking the eight N-terminal residues (CΔ8-Vero cells), a construct previously shown to be unable to complement ΔNotI. In C-Vero cells but not Vero or CΔ8-Vero cells, replication of a wild-type (wt) replicon expressing the green fluorescent protein (GFP) reporter gene (RUBrep/GFP) was enhanced, and replication of a replicon with ΔNotI (RUBrep/GFP-ΔNotI) was rescued. Surprisingly, replicons with deleterious mutations in the 5′ and 3′ cis-acting elements were also rescued in C-Vero cells. Interestingly, the CΔ8 construct localized to the nucleus while the C construct localized in the cytoplasm, explaining the lack of enhancement and rescue in CΔ8-Vero cells since rubella virus replication occurs in the cytoplasm. Enhancement and rescue in C-Vero cells were at a basic step in the replication cycle, resulting in a substantial increase in the accumulation of replicon-specific RNAs. There was no difference in translation of the nonstructural proteins in C-Vero and Vero cells transfected with the wt and mutant replicons, demonstrating that enhancement and rescue were not due to an increase in the efficiency of translation of the transfected replicon transcripts. In replicon-transfected C-Vero cells, C and the P150 replicase protein associated by coimmunoprecipitation, suggesting that C might play a role in RNA replication, which could explain the enhancement and rescue phenomena. A unifying model that accounts for enhancement of wt replicon replication and rescue of diverse mutations by the rubella virus C protein is proposed.

scholarly journals Rubella Virus Capsid Protein Induces Apoptosis in Transfected RK13 Cells

Virology ◽  
2000 ◽  
Vol 275 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Robert Duncan ◽  
Ali Esmaili ◽  
Lok Man J. Law ◽  
Sylvie Bertholet ◽  
Chris Hough ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Structural and Functional Properties of the Capsid Protein of Dengue and Related Flavivirus

2019 ◽  
Vol 20 (16) ◽  
pp. 3870 ◽  
Author(s):  
Faustino ◽  
Martins ◽  
Karguth ◽  
Artilheiro ◽  
Enguita ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Capsid Protein ◽  
Specific Binding ◽  
Protein A ◽  
Terminal Region ◽  
Viral Rna ◽  
Structural Protein ◽  
Virus Capsid ◽  
Structural And Functional Properties ◽  
Virus Capsid Protein

Dengue, West Nile and Zika, closely related viruses of the Flaviviridae family, are an increasing global threat, due to the expansion of their mosquito vectors. They present a very similar viral particle with an outer lipid bilayer containing two viral proteins and, within it, the nucleocapsid core. This core is composed by the viral RNA complexed with multiple copies of the capsid protein, a crucial structural protein that mediates not only viral assembly, but also encapsidation, by interacting with host lipid systems. The capsid is a homodimeric protein that contains a disordered N-terminal region, an intermediate flexible fold section and a very stable conserved fold region. Since a better understanding of its structure can give light into its biological activity, here, first, we compared and analyzed relevant mosquito-borne Flavivirus capsid protein sequences and their predicted structures. Then, we studied the alternative conformations enabled by the N-terminal region. Finally, using dengue virus capsid protein as main model, we correlated the protein size, thermal stability and function with its structure/dynamics features. The findings suggest that the capsid protein interaction with host lipid systems leads to minor allosteric changes that may modulate the specific binding of the protein to the viral RNA. Such mechanism can be targeted in future drug development strategies, namely by using improved versions of pep14-23, a dengue virus capsid protein peptide inhibitor, previously developed by us. Such knowledge can yield promising advances against Zika, dengue and closely related Flavivirus.

Baculovirus-Mediated Large-Scale Expression and Purification of a Polyhistidine-Tagged Rubella Virus Capsid Protein

1998 ◽  
Vol 12 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Michel Schmidt ◽  
Nina Tuominen ◽  
Tove Johansson ◽  
Stefan A. Weiss ◽  
Kari Keinänen ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Large Scale ◽  
Expression And Purification ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Rubella virus capsid protein structure and its role in virus assembly and infection

2013 ◽  
Vol 110 (50) ◽  
pp. 20105-20110 ◽  
Author(s):  
V. Mangala Prasad ◽  
S. D. Willows ◽  
A. Fokine ◽  
A. J. Battisti ◽  
S. Sun ◽  
...  
Keyword(s):  
Protein Structure ◽  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Assembly ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Nucleotide Sequence of the Rubella Virus Capsid Protein Gene Reveals an Unusually High G/C Content

1988 ◽  
Vol 69 (3) ◽  
pp. 603-612 ◽  
Author(s):  
K. Takkinen ◽  
G. Vidgren ◽  
J. Ekstrand ◽  
U. Hellman ◽  
N. Kalkkinen ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Capsid Protein ◽  
Rubella Virus ◽  
Protein Gene ◽  
Capsid Protein Gene ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity

Retrovirology ◽  
2014 ◽  
Vol 11 (1) ◽  
Author(s):  
Martin Obr ◽  
Romana Hadravová ◽  
Michal Doležal ◽  
Ivana Křžová ◽  
Veronika Papoušková ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Protein A ◽  
Virus Capsid ◽  
Critical Step ◽  
Virus Capsid Protein

pH-dependent solubility shift of rubella virus capsid protein

Virology ◽  
1991 ◽  
Vol 181 (2) ◽  
pp. 773-777 ◽  
Author(s):  
Christoph A. Mauracher ◽  
Shirley Gillam ◽  
Robert Shukin ◽  
Aubrey J. Tingle
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Capsid ◽  
Ph Dependent ◽  
Virus Capsid Protein
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