Baculovirus-Mediated Large-Scale Expression and Purification of a Polyhistidine-Tagged Rubella Virus Capsid Protein

1998 ◽  
Vol 12 (3) ◽  
pp. 323-330 ◽  
Author(s):  
Michel Schmidt ◽  
Nina Tuominen ◽  
Tove Johansson ◽  
Stefan A. Weiss ◽  
Kari Keinänen ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Large Scale ◽  
Expression And Purification ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Analysis of Rubella Virus Capsid Protein-Mediated Enhancement of Replicon Replication and Mutant Rescue

2006 ◽  
Vol 80 (8) ◽  
pp. 3966-3974 ◽  
Author(s):  
Wen-Pin Tzeng ◽  
Jason D. Matthews ◽  
Teryl K. Frey
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Fluorescent Protein ◽  
Vero Cells ◽  
Basic Step ◽  
Virus Capsid ◽  
Replicase Protein ◽  
Virus C ◽  
Green Fluorescent ◽  
Virus Capsid Protein

ABSTRACT The rubella virus capsid protein (C) has been shown to complement a lethal deletion (termed ΔNotI) in P150 replicase protein. To investigate this phenomenon, we generated two lines of Vero cells that stably expressed either C (C-Vero cells) or C lacking the eight N-terminal residues (CΔ8-Vero cells), a construct previously shown to be unable to complement ΔNotI. In C-Vero cells but not Vero or CΔ8-Vero cells, replication of a wild-type (wt) replicon expressing the green fluorescent protein (GFP) reporter gene (RUBrep/GFP) was enhanced, and replication of a replicon with ΔNotI (RUBrep/GFP-ΔNotI) was rescued. Surprisingly, replicons with deleterious mutations in the 5′ and 3′ cis-acting elements were also rescued in C-Vero cells. Interestingly, the CΔ8 construct localized to the nucleus while the C construct localized in the cytoplasm, explaining the lack of enhancement and rescue in CΔ8-Vero cells since rubella virus replication occurs in the cytoplasm. Enhancement and rescue in C-Vero cells were at a basic step in the replication cycle, resulting in a substantial increase in the accumulation of replicon-specific RNAs. There was no difference in translation of the nonstructural proteins in C-Vero and Vero cells transfected with the wt and mutant replicons, demonstrating that enhancement and rescue were not due to an increase in the efficiency of translation of the transfected replicon transcripts. In replicon-transfected C-Vero cells, C and the P150 replicase protein associated by coimmunoprecipitation, suggesting that C might play a role in RNA replication, which could explain the enhancement and rescue phenomena. A unifying model that accounts for enhancement of wt replicon replication and rescue of diverse mutations by the rubella virus C protein is proposed.

scholarly journals Rubella Virus Capsid Protein Induces Apoptosis in Transfected RK13 Cells

Virology ◽  
2000 ◽  
Vol 275 (1) ◽  
pp. 20-29 ◽  
Author(s):  
Robert Duncan ◽  
Ali Esmaili ◽  
Lok Man J. Law ◽  
Sylvie Bertholet ◽  
Chris Hough ◽  
...  
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Capsid ◽  
Virus Capsid Protein

scholarly journals Rubella virus capsid protein structure and its role in virus assembly and infection

2013 ◽  
Vol 110 (50) ◽  
pp. 20105-20110 ◽  
Author(s):  
V. Mangala Prasad ◽  
S. D. Willows ◽  
A. Fokine ◽  
A. J. Battisti ◽  
S. Sun ◽  
...  
Keyword(s):  
Protein Structure ◽  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Assembly ◽  
Virus Capsid ◽  
Virus Capsid Protein

Rubella virus capsid protein: a small protein with big functions

2010 ◽  
Vol 5 (4) ◽  
pp. 571-584 ◽  
Author(s):  
Carolina S Ilkow ◽  
Steven D Willows ◽  
Tom C Hobman
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Protein A ◽  
Virus Capsid ◽  
Small Protein ◽  
Virus Capsid Protein

scholarly journals Nucleotide Sequence of the Rubella Virus Capsid Protein Gene Reveals an Unusually High G/C Content

1988 ◽  
Vol 69 (3) ◽  
pp. 603-612 ◽  
Author(s):  
K. Takkinen ◽  
G. Vidgren ◽  
J. Ekstrand ◽  
U. Hellman ◽  
N. Kalkkinen ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Capsid Protein ◽  
Rubella Virus ◽  
Protein Gene ◽  
Capsid Protein Gene ◽  
Virus Capsid ◽  
Virus Capsid Protein

pH-dependent solubility shift of rubella virus capsid protein

Virology ◽  
1991 ◽  
Vol 181 (2) ◽  
pp. 773-777 ◽  
Author(s):  
Christoph A. Mauracher ◽  
Shirley Gillam ◽  
Robert Shukin ◽  
Aubrey J. Tingle
Keyword(s):  
Capsid Protein ◽  
Rubella Virus ◽  
Virus Capsid ◽  
Ph Dependent ◽  
Virus Capsid Protein

scholarly journals Dimerization of Rubella Virus Capsid Protein Is Not Required for Virus Particle Formation

Virology ◽  
1996 ◽  
Vol 216 (1) ◽  
pp. 223-227 ◽  
Author(s):  
JIA YEE LEE ◽  
DOROTHY HWANG ◽  
SHIRLEY GILLAM
Keyword(s):  
Virus Particle ◽  
Capsid Protein ◽  
Rubella Virus ◽  
Particle Formation ◽  
Virus Capsid ◽  
Virus Capsid Protein
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