scholarly journals In vitro regeneration of Anacardium occidentale from shoot tip and basal part

2020 ◽  
Vol 5 (3) ◽  
pp. 621-630
Author(s):  
Bi Tra Jean-Innocent NANTI ◽  
Yah Gwladys GNAMIEN ◽  
Tchoa KONE ◽  
Brahima André SOUMAHORO ◽  
Mongomaké KONE
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Tip ◽  
Anacardium Occidentale ◽  
Basal Part

scholarly journals CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

2015 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Laureen Michelle Houllou ◽  
Robson Antônio de Souza ◽  
Elizabete Cristina Pacheco dos Santos ◽  
José Jackson Pereira da Silva ◽  
Marta Ribeiro Barbosa ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Regenerative Process ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Stem Base ◽  
Different Characteristics

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

scholarly journals In vitro propagation of Andrographis paniculata Nees.-A threatened medicinal plant of Bangladesh

2016 ◽  
Vol 3 (1) ◽  
pp. 67-73
Author(s):  
PK Roy
Keyword(s):  
Activated Charcoal ◽  
In Vitro Regeneration ◽  
Shoot Elongation ◽  
Shoot Tip ◽  
Andrographis Paniculata ◽  
Nodal Segment ◽  
Threatened Medicinal Plant ◽  
Half Strength ◽  
Number Of Shoots

An efficient protocol was developed for in vitro regeneration of plantlets from shoot tip and nodal segment explants of Andrographis paniculata Nees. Nodal segment explants produced the highest number of shoots (18±1.24) when they were cultured on MS supplemented with 11.10 ?M/l BAP. Addition of 10% coconut water and 2.0 g/l activated charcoal to the above mentioned medium increased the number of shoots (30) per culture. Shoot tip explant also showed better performance in the same medium. Addition of 100 mg/l urea and 2.0 g/l activated charcoal to the medium showed proper shoot elongation. The isolated shoots rooted well (90%) on half-strength MS fortified with 9.80 ?M/l IBA, where average number of roots per shoot was 28-30. The plantlets were acclimatized successfully in poly bags containing a mixture of soil, sand and compost in 2:1:1 ratio. Finally acclimatized plantlets were transferred to experimental field.Jahangirnagar University J. Biol. Sci. 3(1): 67-73, 2014 (June)

scholarly journals Impact of Different Explants and Growth Regulators on In vitro Regeneration of Chrysanthemum

2020 ◽  
pp. 10-18
Author(s):  
Md. Nazmul Hasan Mehedi ◽  
Nurunnahar Mitu ◽  
Mahbub Robbani ◽  
Kazi Foyjun Nesa Sukhi ◽  
Md. Hasan Sofiur Rahman ◽  
...  
Keyword(s):  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Tip ◽  
Ms Medium ◽  
Apical Shoot ◽  
Standardized Protocol ◽  
Best Response ◽  
The Family ◽  
Number Of Leaves

Chrysanthemum is the world’s second most economically important flower crop and commonly known as ‘Autumn Queen’. It belongs to the family Compositeae (Asteraceae). It is native to Asia and northeastern Europe and has been cultivated for more than 2000 years. The present study within vitro regeneration of chrysanthemum was carried out to develop the standardized protocol for organogenesis. In this study, three types of explants viz. apical shoot tip, internodal segment and young leaf along with different concentrations and combinations of growth regulators were used for in vitro regeneration. BAP and KIN were used for in vitro microshoot regeneration and IBA along with 2, 4-D were used for in vitro microroot regeneration. Minimum days (7.00) for microshoot initiation, maximum microshoot initiation percentage (97.00), highest number of microshoot per plantlet (12.00), highest number of leaves per microshoot (14.60) and maximum microshoot length (4.60) at 28 DAC were recorded as best performances by apical shoot tip inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L. On the other hand, minimum days (5.00) for microroot initiation, maximum microroot initiation percentage (97.60), the highest number of microroots per plantlet (11.80) and maximum microroot length (6.20) were obtained from apical shoot tip inoculated into ½ strength MS medium supplemented with IBA 0.2 mg/L + 2, 4-D 0.1 mg/L. In case of microshoot regeneration, the best response was showed by apical shoot tip when inoculated into MS medium supplemented with BAP 2.5 mg/L + KIN 0.5 mg/L and the microrooting of plantlets were best from apical shoot tip inoculated into ½ MS medium supplemented with IBA 0.2 mg/L along with 2, 4-D 0.1 mg/L.

scholarly journals In Vitro Regeneration of Coral Tree from Three Different Explants Using Thidiazuron

2019 ◽  
Vol 29 (6) ◽  
pp. 946-951 ◽  
Author(s):  
Saad B. Javed ◽  
Abdulrahman A. Alatar ◽  
Mohammad Anis ◽  
Mohamed A. El-Sheikh
Keyword(s):  
In Vitro Regeneration ◽  
Adventitious Shoots ◽  
Issr Markers ◽  
Shoot Tip ◽  
Shoot Number ◽  
Efficient Alternative ◽  
Coral Tree ◽  
Hypocotyl Tissue ◽  
The Cost

The coral tree (Erythrina variegata) is a multipurpose horticultural plant with a plethora of medicinally important alkaloids. Regeneration via tissue culture can provide an efficient alternative to seed-grown plantlets and reduce the cost of the plant significantly. Thidiazuron (TDZ) is an efficient plant growth regulator and is effective in numerous species. However, the response to it varies with the type and position of the tissue on the plantlet treated. This study was carried out to ascertain the best tissue types for micropropagation of the coral tree using TDZ. Three tissue types (shoot tip, nodal, and hypocotyl), originating from different strata of the plantlet were evaluated. Adventitious shoots were observed in all three explants at the tested concentrations. However the quality and the shoot number varied significantly with the type of explant. Explants with a meristematic zone (shoot tip and nodal) were more responsive to the treatment compared with hypocotyl tissue lacking preexisting meristem. Nodal explants produced the maximum number of shoots (about eight) per explant after 4 weeks of culture, whereas shoot tips produced about only five shoots per explant at an equimolar concentration (1.5 µm). Approximately three shoots were observed in hypocotyl explants. Moreover, growth and rooting of the regenerated shoots was influenced by the origin of the explants. The molecular characterization of the regenerants using intersimple sequence repeat (ISSR) markers revealed genetic homogeneity among regenerants. An efficient micropropagation method for the coral tree is described.

In Vitro Regeneration of Banana Cv. Nanjanagud Rasabale (AAB) by Shoot Tip Culture

2018 ◽  
Vol 20 (4) ◽  
pp. 1-9
Author(s):  
Prabhuling Guranna ◽  
Rashmi Hegde ◽  
Babu Govindaswamy
Keyword(s):  
In Vitro Regeneration ◽  
Shoot Tip ◽  
Shoot Tip Culture
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