scholarly journals In-vitro screening of Iranian Landraces (Triticumaestivum L.) at the seedling stage for water stress tolerance using Polyethylene glycol (PEG 6000)

2020 ◽  
Vol 5 (1) ◽  
pp. 130-135
Author(s):  
Amandeep Kaur ◽  
Rashpal Singh Sarlach
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Stress Tolerance ◽  
Seedling Stage ◽  
In Vitro Screening ◽  
Peg 6000 ◽  
Water Stress Tolerance

Improving water stress tolerance of the biocontrol yeastCandida sakegrown in molasses-based media by physiological manipulation

2001 ◽  
Vol 47 (2) ◽  
pp. 123-129 ◽  
Author(s):  
M Abadias ◽  
N Teixidó ◽  
J Usall ◽  
I Viñas ◽  
N Magan
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Amino Acid ◽  
Cell Viability ◽  
Stress Tolerance ◽  
Biocontrol Agent ◽  
Compatible Solutes ◽  
Intracellular Concentration ◽  
Water Stress Tolerance ◽  
Candida Sake

The biocontrol agent Candida sake was cultured on either an unmodified molasses-based medium (water activity, aw0.996) or on water stressed media produced by the addition of glycerol, glucose, NaCl, sorbitol, or proline to 0.98, and 0.96 awfor 24, 48, and 72 h, to study their impact on subsequent cell viability, and on concentrations of endogenous sugars (trehalose and glucose) and polyols (glycerol, erythritol, arabitol, and mannitol). The viability of cells of different ages cultured on these media was evaluated on NYDA medium with freely available water (aw0.995), and on medium modified with polyethylene glycol to aw0.95. Regardless of solute used, viable counts of cells grown on molasses-based medium (aw0.98) were equal to or higher than those obtained from the medium with water freely available. The amino acid proline stimulated growth at 10% concentration. In contrast, water stress induced by addition of NaCl, glucose, or sorbitol at aw0.96 caused a significant reduction in viable counts. Older cultures were more resistant to water stress. Glycerol and arabitol were the main solutes accumulated by C. sake cells in response to lowered aw. Intracellular concentration of these polyols depended more on the solute used to adjust the awthan on the awitself. Candida sake was more resistant to water stress with higher intracellular concentration of glycerol and erythritol.Key words: compatible solutes, polyols, sugars, improved viability, formulation.

Cell wall proteins of in vitro cultured chili pepper lines differing in water stress tolerance

Plant Science ◽  
1997 ◽  
Vol 128 (2) ◽  
pp. 217-223 ◽  
Author(s):  
Quintero-Higuera Marı́a Fernanda ◽  
Santos-Dı́az Marı́a del Socorro ◽  
Garcı́a-de la Cruz Ramón Fernando
Keyword(s):  
Cell Wall ◽  
Water Stress ◽  
Stress Tolerance ◽  
Chili Pepper ◽  
Cell Wall Proteins ◽  
Water Stress Tolerance

Enhancing water stress tolerance in pepper at seedling stage by 24-epibrassinolid (EBL) applications

2016 ◽  
pp. 109-416
Author(s):  
A. Samancıoğlu ◽  
F. Kocaçınar ◽  
A.R. Demirkıran ◽  
A. Korkmaz
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Seedling Stage ◽  
Water Stress Tolerance

Antioxidant system response of different wheat cultivars under drought: field and in vitro studies

2001 ◽  
Vol 28 (11) ◽  
pp. 1095 ◽  
Author(s):  
Hernán R. Lascano ◽  
Gerardo E. Antonicelli ◽  
Celina M. Luna ◽  
Mariana N. Melchiorre ◽  
Leonardo D. Gómez ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Tolerance ◽  
Oxidative Damage ◽  
Stress Tolerance ◽  
Antioxidant System ◽  
System Response ◽  
Wheat Cultivars ◽  
System Activity ◽  
Water Stress Tolerance

The participation of the antioxidant system in the drought tolerance of wheat cultivars (Triticum aestivum L.) was studied under field and in vitro conditions. Under field conditions, drought tolerance was evaluated by the capacity to maintain the grain yield under drought, which was higher in cvv. Elite and La Paz than in the sensitive cvv. Oasis and Cruz Alta. Tolerant cultivars showed lower relative water content (RWC) and lower above-ground vegetative biomass than sensitive cultivars. Field assays did not show a clear correlation between water-stress tolerance and antioxidant system behaviour. However, when leaves of cvv. with contrasting drought tolerance were subjected to osmotic stress in vitro, clear differences in the antioxidant system activity and oxidative damage between cvv. were observed. In the tolerant cultivar Elite, it was possible to observe an increase in ascorbate peroxidase (APX), superoxide dismutase (SOD) and glutathione reductase (GR) activities, a higher glutathione (GSH) and ascorbate content and less oxidative damage than in the sensitive cultivar Oasis, which showed no changes or only slight decreases in the enzyme activities. These results indicate that water stress tolerance is in part associated with the antioxidant system activity, and suggest that the behaviour of the antioxidant systemin vitro assays can be used as an early selection tool.

scholarly journals In vitro screening of durum wheat against water-stress mediated through polyethylene glycol

2017 ◽  
Vol 15 (1) ◽  
pp. 239-247 ◽  
Author(s):  
Nadia Sandra Kacem ◽  
Fabienne Delporte ◽  
Yordan Muhovski ◽  
Abdelhamid Djekoun ◽  
Bernard Watillon
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Durum Wheat ◽  
In Vitro Screening

scholarly journals Genome-Driven Investigation of Compatible Solute Biosynthesis Pathways of Pseudomonas syringae pv. syringae and Their Contribution to Water Stress Tolerance

2010 ◽  
Vol 76 (16) ◽  
pp. 5452-5462 ◽  
Author(s):  
Matthias Kurz ◽  
Adrien Y. Burch ◽  
Britta Seip ◽  
Steven E. Lindow ◽  
Harald Gross
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Pseudomonas Syringae ◽  
Compatible Solutes ◽  
Small Contribution ◽  
In Planta ◽  
Putative Gene ◽  
Fusion Strain ◽  
Water Stress Tolerance

ABSTRACT The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, and biochemical approaches to address water stress tolerance in P. syringae pv. syringae strain B728a, for which a complete genome sequence is available. P. syringae pv. syringae B728a is able to produce the compatible solutes betaine, ectoine, N-acetylglutaminylglutamine amide (NAGGN), and trehalose. Analysis of osmolyte profiles of P. syringae pv. syringae B728a under a variety of in vitro and in planta conditions reveals that the osmolytes differentially contribute to water stress tolerance in this species and that they interact at the level of transcription to yield a hierarchy of expression. While the interruption of a putative gene cluster coding for NAGGN biosynthesis provided the first experimental evidence of the NAGGN biosynthetic pathway, application of this knockout strain and also a gfp reporter gene fusion strain demonstrated the small contribution of NAGGN to cell survival and desiccation tolerance of P. syringae pv. syringae B728a under in planta conditions. Additionally, detailed investigation of ectC, an orphan of the ectoine cluster (lacking the ectA and ectB homologs), revealed its functionality and that ectoine production could be detected in NaCl-amended cultures of P. syringae pv. syringae B728a to which sterilized leaves of Syringa vulgaris had been added.

scholarly journals In Vitro Screening of Sugarcane Cultivars (Saccharum spp. Hybrids) for Tolerance to Polyethylene Glycol-Induced Water Stress

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 598
Author(s):  
César A. Hernández-Pérez ◽  
Fernando Carlos Gómez-Merino ◽  
José L. Spinoso-Castillo ◽  
Jericó J. Bello-Bello
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
In Vitro Screening ◽  
Sugarcane Crop ◽  
Drought Tolerant ◽  
Solid Culture Medium ◽  
Use Efficiency ◽  
Semi Solid

Water stress caused by drought affects the productivity of the sugarcane crop. A breeding alternative is the selection of drought-tolerant sugarcane cultivars. The objective of this study was the in vitro screening of cultivars tolerant to water stress using polyethylene glycol (PEG) as a stressing agent. Cultivars (cv) Mex 69-290, CP 72-2086, Mex 79-431 and MOTZMex 92-207 were subjected to different concentrations of PEG 6000 (0, 3, 6 and 9% w/v) using Murashige and Skoog semi-solid culture medium. At 30 days of culture, different developmental variables and dry matter (DM), total protein (TP), proline (Pr) and glycine-betaine (GB) contents were evaluated. The results showed reduced development in cv CP 72-2086, Mex 79-431 and MOTZMex 92-207 with increasing PEG concentration. The cv Mex 69-290 showed tolerance to osmotic stress of −0.45 MPa using 3% PEG. Overall, TP content decreased with increasing PEG concentration, while DM, Pr and GB contents rose with increasing PEG concentration in all evaluated cultivars. Our results suggest that cv Mex 69-290 has a slight tolerance to water stress and could be used for rainfed cultivation with low rainfall or reduced irrigation for better water use efficiency. In conclusion, the in vitro screening technique of cultivars tolerant to PEG-induced water stress is an alternative for early determination of drought stress in sugarcane.

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