Improving water stress tolerance of the biocontrol yeastCandida sakegrown in molasses-based media by physiological manipulation

2001 ◽  
Vol 47 (2) ◽  
pp. 123-129 ◽  
Author(s):  
M Abadias ◽  
N Teixidó ◽  
J Usall ◽  
I Viñas ◽  
N Magan
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Amino Acid ◽  
Cell Viability ◽  
Stress Tolerance ◽  
Biocontrol Agent ◽  
Compatible Solutes ◽  
Intracellular Concentration ◽  
Water Stress Tolerance ◽  
Candida Sake

The biocontrol agent Candida sake was cultured on either an unmodified molasses-based medium (water activity, aw0.996) or on water stressed media produced by the addition of glycerol, glucose, NaCl, sorbitol, or proline to 0.98, and 0.96 awfor 24, 48, and 72 h, to study their impact on subsequent cell viability, and on concentrations of endogenous sugars (trehalose and glucose) and polyols (glycerol, erythritol, arabitol, and mannitol). The viability of cells of different ages cultured on these media was evaluated on NYDA medium with freely available water (aw0.995), and on medium modified with polyethylene glycol to aw0.95. Regardless of solute used, viable counts of cells grown on molasses-based medium (aw0.98) were equal to or higher than those obtained from the medium with water freely available. The amino acid proline stimulated growth at 10% concentration. In contrast, water stress induced by addition of NaCl, glucose, or sorbitol at aw0.96 caused a significant reduction in viable counts. Older cultures were more resistant to water stress. Glycerol and arabitol were the main solutes accumulated by C. sake cells in response to lowered aw. Intracellular concentration of these polyols depended more on the solute used to adjust the awthan on the awitself. Candida sake was more resistant to water stress with higher intracellular concentration of glycerol and erythritol.Key words: compatible solutes, polyols, sugars, improved viability, formulation.

scholarly journals Drought Stress Impacts on Plants and Different Approaches to Alleviate Its Adverse Effects

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 259
Author(s):  
Mahmoud F. Seleiman ◽  
Nasser Al-Suhaibani ◽  
Nawab Ali ◽  
Mohammad Akmal ◽  
Majed Alotaibi ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Adverse Effects ◽  
Stress Tolerance ◽  
Hormonal Regulation ◽  
Plant Biomass ◽  
Compatible Solutes ◽  
Plant Tolerance ◽  
Water Stress Tolerance ◽  
Adaptation Mechanisms

Drought stress, being the inevitable factor that exists in various environments without recognizing borders and no clear warning thereby hampering plant biomass production, quality, and energy. It is the key important environmental stress that occurs due to temperature dynamics, light intensity, and low rainfall. Despite this, its cumulative, not obvious impact and multidimensional nature severely affects the plant morphological, physiological, biochemical and molecular attributes with adverse impact on photosynthetic capacity. Coping with water scarcity, plants evolve various complex resistance and adaptation mechanisms including physiological and biochemical responses, which differ with species level. The sophisticated adaptation mechanisms and regularity network that improves the water stress tolerance and adaptation in plants are briefly discussed. Growth pattern and structural dynamics, reduction in transpiration loss through altering stomatal conductance and distribution, leaf rolling, root to shoot ratio dynamics, root length increment, accumulation of compatible solutes, enhancement in transpiration efficiency, osmotic and hormonal regulation, and delayed senescence are the strategies that are adopted by plants under water deficit. Approaches for drought stress alleviations are breeding strategies, molecular and genomics perspectives with special emphasis on the omics technology alteration i.e., metabolomics, proteomics, genomics, transcriptomics, glyomics and phenomics that improve the stress tolerance in plants. For drought stress induction, seed priming, growth hormones, osmoprotectants, silicon (Si), selenium (Se) and potassium application are worth using under drought stress conditions in plants. In addition, drought adaptation through microbes, hydrogel, nanoparticles applications and metabolic engineering techniques that regulate the antioxidant enzymes activity for adaptation to drought stress in plants, enhancing plant tolerance through maintenance in cell homeostasis and ameliorates the adverse effects of water stress are of great potential in agriculture.

scholarly journals Genome-Driven Investigation of Compatible Solute Biosynthesis Pathways of Pseudomonas syringae pv. syringae and Their Contribution to Water Stress Tolerance

2010 ◽  
Vol 76 (16) ◽  
pp. 5452-5462 ◽  
Author(s):  
Matthias Kurz ◽  
Adrien Y. Burch ◽  
Britta Seip ◽  
Steven E. Lindow ◽  
Harald Gross
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Pseudomonas Syringae ◽  
Compatible Solutes ◽  
Small Contribution ◽  
In Planta ◽  
Putative Gene ◽  
Fusion Strain ◽  
Water Stress Tolerance

ABSTRACT The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, and biochemical approaches to address water stress tolerance in P. syringae pv. syringae strain B728a, for which a complete genome sequence is available. P. syringae pv. syringae B728a is able to produce the compatible solutes betaine, ectoine, N-acetylglutaminylglutamine amide (NAGGN), and trehalose. Analysis of osmolyte profiles of P. syringae pv. syringae B728a under a variety of in vitro and in planta conditions reveals that the osmolytes differentially contribute to water stress tolerance in this species and that they interact at the level of transcription to yield a hierarchy of expression. While the interruption of a putative gene cluster coding for NAGGN biosynthesis provided the first experimental evidence of the NAGGN biosynthetic pathway, application of this knockout strain and also a gfp reporter gene fusion strain demonstrated the small contribution of NAGGN to cell survival and desiccation tolerance of P. syringae pv. syringae B728a under in planta conditions. Additionally, detailed investigation of ectC, an orphan of the ectoine cluster (lacking the ectA and ectB homologs), revealed its functionality and that ectoine production could be detected in NaCl-amended cultures of P. syringae pv. syringae B728a to which sterilized leaves of Syringa vulgaris had been added.

Rhizosphere Symbionts Improve Water Stress Tolerance in Moldavian balm through Modulation of Osmolytes

Rhizosphere ◽  
2021 ◽  
pp. 100367
Author(s):  
Zohreh Ghanbarzadeh ◽  
Hajar Zamani ◽  
Sasan Mohsenzadeh ◽  
Łukasz Marczak ◽  
Maciej Stobiecki ◽  
...  
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Water Stress Tolerance

Screening for water stress tolerance in eleven plum (Prunussalicina L.) Cultivars using agronomic and physiological traits

2021 ◽  
Vol 281 ◽  
pp. 109992
Author(s):  
Anas Hamdani ◽  
Jamal Charafi ◽  
Said Bouda ◽  
Lahcen Hssaini ◽  
Atman Adiba ◽  
...  
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Physiological Traits ◽  
Water Stress Tolerance

LIFE HISTORY TYPE AND WATER STRESS TOLERANCE IN NINE CALIFORNIA CHAPARRAL SPECIES (RHAMNACEAE)

2007 ◽  
Vol 77 (2) ◽  
pp. 239-253 ◽  
Author(s):  
R. B. Pratt ◽  
A. L. Jacobsen ◽  
K. A. Golgotiu ◽  
J. S. Sperry ◽  
F. W. Ewers ◽  
...  
Keyword(s):  
Life History ◽  
Water Stress ◽  
Stress Tolerance ◽  
Water Stress Tolerance

scholarly journals Estimation of water stress tolerance of six woody plant species

2021 ◽  
Vol 5 (2) ◽  
pp. 64-72
Author(s):  
Danesha Seth Carley ◽  
Lauren A Gragg ◽  
Matthew J Matthew ◽  
Thomas W Rufty
Keyword(s):  
Water Stress ◽  
Stress Tolerance ◽  
Plant Species ◽  
Woody Plant ◽  
Woody Plant Species ◽  
Water Stress Tolerance
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