scholarly journals SENSITIFITAS UDANG Litopenaeus stylirostris DAN Penaeus monodon TERHADAP INFEKSI SEMBV (SYSTEMIC ECTODERMAL AND MESODERMAL BACULOVIRUS)

2002 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
A. Taslihan ◽  
R. Handayani ◽  
Suryati Suryati ◽  
N. Fahris
Keyword(s):  
Disease Virus ◽  
Penaeus Monodon ◽  
Shrimp Species ◽  
Tiger Shrimp ◽  
White Spots ◽  
Sensitivity Level ◽  
Tiger Prawn ◽  
Serious Disease

Study on sensitifity of two shrimps species, there were blue shrimp (Litopenaeus stylirostris) and giant tiger shrimp (Penaeus monodon) against local isolat of SEMBV (systemic ectodermal and mesodermal baculovirus) has been undertaken. The objective of  the study were to know sensitivity level of the two shrimp species to virus that cause serious disease namely WSDV (white spots disease virus). Result from the study showed that both shrimps are sensitive to the virus. Inoculating virus through injection caused  transmission faster than that by feeding shrimp with infected carcass. Blue shrimp also showed relatively sensitive to SEMBV then that of giant tiger prawn.

scholarly journals KONSERVASI SUMBER DAYA UDANG WINDU (Penaeus monodon) DI PANTAI TIMUR ACEH, KABUPATEN ACEH TIMUR

2019 ◽  
Vol 1 (1) ◽  
pp. 39
Author(s):  
Didik Wahju Hendro Tjahjo ◽  
Dimas Angga Hedianto ◽  
Astri Suryandari
Keyword(s):  
Habitat Degradation ◽  
Risk Estimation ◽  
Economic Value ◽  
Penaeus Monodon ◽  
Institutional Development ◽  
Shrimp Farming ◽  
Tiger Shrimp ◽  
Tiger Prawn ◽  
In The Wild ◽  
Larvae Density

Udang windu (Penaeus monodon) merupakan salah satu komoditas udang utama dan mempunyai nilai ekonomi tinggi di perairan, Kabupaten Aceh. Saat ini laju eksploitasi udang windu sangat tinggi. Hal tersebut merupakan ancaman terhadap kelestarian sumber daya udang windu. Tujuan penelitian ini untuk mengkaji opsi pengelolaan konservasi udang windu di Aceh Timur. Kepadatan post larva Penaeidae berkisar antara 0-214 ind/1.000 m3 dan kepadatan stok juvenil udang windu berkisar antara 686-1.875 ind/km2, dimana kepadatan tertinggi di Kuala Arakundo dan Kuala Peureulak. Distribusi spasial kelimpahan udang windu berkisar antara 10-130 ekor/m2 (10-167.000 g/ha). Analisis aspek status pemanfaatan, degradasi habitat (penebangan liar, pembukaan tambak, dan sedimentasi), perkiraan dan evaluasi resiko, serta faktor-faktor yang mendukung diantaranya respon masyarakat dan kesiapan sistem sosial merupakan masukan dalam menentukan konservasi sumber daya udang windu. Oleh karena itu, dalam upaya menjamin kelestarian sumber daya udang windu di alam serta keberlanjutan usaha budidayanya, perlu dilakukan langkah-langkah pengelolaan dan konservasi sumber daya udang windu yang rasional, seperti (a) pengendalian dan pemulihan degradasi lingkungan melalui pengendalian erosi bagian hulu-hilir, dan menjaga dan merehabilitasi hutan mangrove, (b) pengendalian penyebaran penyakit dengan pendekatan kehati-hatian untuk pengembangan budidaya udang vanamei, (c) pengendalian penangkapan juvenil udang windu melalui pelarangan beroperasi alat tangkap sejenis trawl (pukat langgih dan pukat layang), dan (d) revitalisasi dan pengembangan kelembagaan nelayan.Indian tiger prawn (Penaeusmonodon) is one of the main shrimp commodities and has high economic value. East coastal waters of East Aceh district is known as one of the main producer of tiger prawns with the best quality. On the other hand the rate of exploitation of tiger shrimp is very high. This issue is therefore need to be a addressed further. The purpose of this study is, therefore, to assess the management activities needed to conserve tiger shrimp in East Aceh. Penaeidae post larvae density in Aceh Timur ranges from 0-214 ind/1,000 m3 and juvenile stock density ranges from 0,245-49,419 kg/km2, where the highest density is in Kuala Arakundo and Kuala Peureulak. The Spatial Abundance Distribution of indian tiger prawn ranges from 10 to 130 ind./m2 (10-167,000 g/ha). Analysis of aspects of utilization status, habitat degradation (illegal logging, sedimentation, land clearing for aquaculture), risk estimation and evaluation, and factors are inputthat support conservation of tiger shrimp resources. Therefore, in an effort to ensure the sustainability of tiger shrimp resources in the wild and the sustainability of its cultivation business, it is necessary to take steps to manage and conserve rational tiger shrimp resources.such as (a) controlling and restoring environmental degradation through upstream-downstream erosion control, and safeguarding and rehabilitating mangrove, (b) controlling the spread of diseases with precautionary approach to the development of vanamei shrimp farming, (c) controlling the capture of indian tiger prawn juveniles through the prohibition operates of bottom trawling (pukat langgih and pukat layang), and (d) fisherman revitalization and institutional development.

scholarly journals Effect of Heating at Various Temperatures for 30 Minutes on Pathogenicity of White Spot Syndrome Virus (WSSV) in Tiger Prawn (Penaeus monodon Fabr.)

2007 ◽  
Vol 5 (1) ◽  
pp. 5
Author(s):  
D. Priatni ◽  
M. Alifuddin ◽  
D. Djokosetiyanto
Keyword(s):  
Survival Rate ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Tiger Shrimp ◽  
Pathogenic Virus ◽  
Short Period ◽  
Tiger Prawn ◽  
White Spot Syndrome

<p>White spot syndrome virus<em> </em>(WSSV) is a strong pathogenic virus which spread very rapidly and can cause tiger shrimp mass mortality within a short period.  Enhancement of shrimp immunity by infecting inactivated WSSV is one of the efforts to overcome WSSV infection in shrimp.  In this study, inactivated WSSV were prepared by heating them with various temperatures namely 45, 50, 55 and 60<sup>o</sup>C for 30 minutes. The results shows that infection with  heating inactivated WSSV at 45°C and 60°C for 30 min on PL-15 could increase their immunities.  The survival rate of inactivated WSSV-infected shrimp after challenge test with  WSSV virulent  reached 77%, while  no survive shrimp was observed in control.  This suggests that shrimp immunity could be improved by vaccination using  WSSV virus inactivated by heating.</p> <p>Keywords: WSSV virus, pathogen, tiger shrimp, heating</p> <p> </p> <p>ABSTRAK</p> <p><em>White Spot Syndrome Virus </em>(WSSV) merupakan virus yang sangat ganas bagi udang windu, dengan penularan yang sangat cepat dan menyebabkan kematian dalam waktu yang cepat. Peningkatan imunitas udang dengan meenginfeksikan WSSV inaktif merupakan salah satu upaya untuk menanggulangi infeksi WSSV pada udang. Pada penelitian ini, inaktivasi WSSV dilakukan menggunakan pemanasan pada suhu berbeda, yaitu 45, 50, 55 dan 60<sup>o</sup>C selama 30 menit.  Hasil penelitian menunjukkan bahwa infeksi PL-15 menggunakan virus WSSV yang telah diinaktivasi dengan pemanasan pada suhu 45<sup>o</sup>C dan 60<sup>o</sup>C dapat meningkatkan daya tahan udang.  Kelangsungan hidup udang yang telah diinfeksi dengan WSSV hasil inaktivasi sebelum uji tantang dengan WSSV virulen mencapai 77%, sementara udang yang tidak diinfeksi dengan WSSV hasil inaktivasi adalah semua mati.  Dengan demikian dapat disimpulkan bahwa daya tahan udang dapat ditingkatkan melalui vaksinasi menggunakan WSSV yang telah diinaktivasi dengan pemanasan.</p> <p>Kata kunci: virus WSSV, patogen, udang windu, pemanasan</p>

scholarly journals Penggunaan Pakan Bermethamorfosis Pada Perbenihan Udang Windu Penaeus monodon Di Kabupaten Barru

2021 ◽  
Vol 21 (2) ◽  
pp. 358-373
Author(s):  
Sutia Budi ◽  
Amal Aqmal
Keyword(s):  
Seed Production ◽  
Penaeus Monodon ◽  
International Markets ◽  
Industrial Scale ◽  
Feed Formulation ◽  
Seed Technology ◽  
South Sulawesi ◽  
Tiger Shrimp ◽  
Tiger Prawn

Perkembangan perbenihan udang windu mengalami kemajuan yang sangat pesat, hal ini didukung oleh usaha budidaya yang intensif dengan penerapan teknologi perbenihan yang cukup meningkat, harga yang tinggi dipasar lokal maupun internasional, dan peluang yang luas telah membuat udang windu menjadi komoditas harapan bagi para pengusaha sehingga banyak yang berani menanamkan modal bisnis udang windu ini. Kegiatan pembenihan di Sulawesi Selatan khususnya Desa Kupa Kabupaten Barru merupakan sentra pengembangan Pembenihan Udang Windu. Kegiatan pembenihan mulai dari skala rumah tangga sampai industri. Teknologi dan formulasi pakan yang dikembangkan oleh kelompok dan masyarakat perbenihan belum optimal dalam mendukung peningkatan produksi benih Udang Windu sehingga  diperlukan paket teknologi dan formulasi pakan dalam pembenihan udang windu sebagai salah satu solusi dalam optimalisasi peningkatan produksi. The development of tiger shrimp hatchery is progressing very rapidly, this is supported by intensive cultivation efforts with the application of improved seed technology, high prices in local and international markets, and wide opportunities that have made tiger prawns a commodity of hope for entrepreneurs. who dared to invest in this tiger prawn business. Hatchery activities in South Sulawesi, especially Kupa Village, Barru Regency are the center for the development of Windu Shrimp Hatchery. Hatchery activities range from household to industrial scale. Technology and feed formulation developed by hatchery groups and communities have not been optimal in supporting the increase in tiger shrimp seed production, so technology packages and feed formulations in tiger shrimp hatchery are needed as a solution in optimizing increased production.

scholarly journals Effect of Ultraviolet Radiation on Pathogenity of White Spot Syndrome Virus in Giant Tiger Prawn (Penaeus monodon Fab.)

2007 ◽  
Vol 4 (1) ◽  
pp. 79
Author(s):  
M. Subkhan ◽  
M. Alifuddin ◽  
A. Taslihan
Keyword(s):  
Uv Irradiation ◽  
White Spot Syndrome Virus ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
White Spot ◽  
Virus Suspension ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp ◽  
Tiger Prawn ◽  
White Spot Syndrome

<p>This experiment was conducted to examine the effect of UV irradiation on pathogenity of white spot syndrome virus (WSSV) in black tiger shrimp (<em>Penaeus monodon</em> Fab.).  A hundred ml of WSSV virus suspension (200 μg/ml) were placed at 30 cm under UV light 10 Watt.  Radiation on WSSV virus was performed for 15, 30, 45, and 60 minutes.  Black tiger sjrimp in density of 260 tails/L were immersed in 1000 ml of irradiated virus suspension (20 μg/ml) to test their pathogenities.  The results of study showed that duration of UV irradiation on WSSV virus was reverse correlated to their pathogenities.  In constrast, survival of black tiger shrimp was linear correlated to duration of UV inactivation of virus.  Higher survival rate of shrimp (65.52%) after challenge test was obtained by irradiation of virus for 60 min.</p> <p>Keywords:  WSSV, virus, pathogen, ultraviolet, black tiger prawn, <em>Penaeus monodon</em></p> <p> </p> <p>ABSTRAK</p> <p>Penelitian ini dilakukan untuk mengetahui pengaruh radiasi UV terhadap patogenitas virus <em>White Spot Syndrome Virus</em> (WSSV) pada udang windu (<em>Penaeus monodon </em>Fab).   Suspensi virus WSSV dengan konsentrasi 200 μg/ml sebanyak 100 ml ditempatkan 30 cm diradiasi menggunakan UV 10 Watt.  Radiasi dilakukan selama 15, 30, 45, dan 60 menit.  Udang dengan kepadatan 260 ekor/L direndam dalam 1000 ml suspensi virus (20 μg/ml) hasil radiasi untuk menguji patogenitasnya.  Hasil penelitian menunjukkan bahwa lama radiasi UV pada virus WSSV berbanding terbalik dengan tingkat patogenitasnya.  Sementara itu, lama inaktivasi virus dengan UV berbanding lurus terhadap kelangsungan hidup udang.  Kelangsungan hidup udang windu tertinggi (65,52%) setelah uji tantang diperoleh dengan meradiasi virus selama 60 menit.</p> <p>Kata kunci: WSSV, virus, patogen, ultraviolet, udang windu,  <em>Penaeus monodon</em></p>

scholarly journals Growth and survival of post larvae of tiger shrimp (Penaeus monodon Fabr.) through the administration of probiotics with different doses

2017 ◽  
Vol 1 (1) ◽  
pp. 19 ◽  
Author(s):  
Ali Usman ◽  
Rochmady Rochmady
Keyword(s):  
Survival Rate ◽  
Pathogenic Bacteria ◽  
Penaeus Monodon ◽  
Nutrient Content ◽  
Probiotic Bacteria ◽  
Growth And Survival ◽  
Tiger Shrimp ◽  
Tiger Prawn ◽  
Different Doses

One of the factors that affect the growth of tiger shrimp is the quality of feed. Nutrient content in feed such as protein, carbohydrate and fat, generally used as a parameter of feed quality. Feed consumed by shrimp is not all digested, one of which depends on the enzymatic ability of the shrimp digestive tract. The use of probiotic bacteria in addition to having the ability to reduce colonies, inhibit the growth of pathogenic bacteria, and neutralize water quality, probiotic bacteria can help digestibility of the intestine. The aim of this research is to know the growth and survival of post larvae of shrimp (Penaeus monodon Fabr.) Through the provision of probiotics with different doses. The research was conducted at Balai Fish Fishing Hall of Muna Regency, Southeast Sulawesi, Indonesia. Post larva shrimp tiger (PL-20) of 120 tails, maintained for 28 days. The tested treatment was probiotic dose 5 ml (treatment A), dose 10 ml treatment B), dose 15 ml (treatment C), and without probiotics (control). Probiotics are given every 7 days on water maintenance media using a pipette (0.5-20 ml scale). Weighing results showed relatively high post-larva shrimp larvae growth of 400.00% (treatment B); 291.67% (treatment C); of 233.33% (treatment A) and equal to 58.33% (Control). Variation analysis result showed that giving of probiotic with different dose gave real effect (P<0,01) to relative growth of postlarva prawn weights (P. monodon Fabr.). Provision of probiotics has no significant effect on postlarva tiger prawn survival rate (P. monodon Fabr.). The survival rate of post larvae of tiger shrimp during the study was in the range 66.67-83.33%.

scholarly journals De novo assembly, characterization, functional annotation and expression patterns of the black tiger shrimp (Penaeus monodon) transcriptome

2018 ◽  
Author(s):  
Roger Huerlimann ◽  
Nicholas M Wade ◽  
Lavinia Gordon ◽  
Juan D Montenegro ◽  
Jake Goodall ◽  
...  
Keyword(s):  
Life History ◽  
Early Life ◽  
Penaeus Monodon ◽  
Early Life History ◽  
Model Organisms ◽  
Life History Stage ◽  
Black Tiger Shrimp ◽  
Shrimp Species ◽  
Life History Stages ◽  
Tiger Shrimp

AbstractThe black tiger shrimp (Penaeus monodon) remains the second most widely cultured shrimp species globally. However, issues with disease and domestication have seen production levels stagnate over the past two decades. To help identify innovative solutions needed to resolve bottlenecks hampering the culture of this species, it is important to generate genetic and genomic resources. Towards this aim, we have produced the most complete publicly available P. monodon transcriptome database to date. The assembly was carried out in multiple assemblers using 2×125 bp HiSeq data from PolyA selected, ribo-depleted RNA extracted from nine adult tissues and eight early life-history stages. In total, approximately 700 million high-quality sequence reads were obtained and assembled into 236,388 clusters. These were then further segregated into 99,203 adult tissue specific clusters, and 58,678 early life-history stage specific clusters. The final transcriptome had a high TransRate score of 0.37, with 88% of all reads successfully mapping back to the transcriptome. BUSCO statistics showed the assembly to be highly complete with low fragmentation, few genes missing, but higher redundancy or transcript duplication (Complete: 98.2% (Duplicated: 51.3%), Fragmented: 0.8%, Missing: 1.0%), and to greatly exceed the completeness of existing P. monodon transcriptomes. While annotation rates were low (approximately 30%), as is typical for a non-model organisms, annotated transcript clusters were successfully mapped to several hundred functional KEGG pathways. To help address the lack of annotation, transcripts were clustered into groups within tissues and early life-history stages, providing initial evidence for their roles in specific tissue functions, or developmental transitions. Additionally, transcripts of shrimp viruses previously not known to occur in Australia were also discovered. We expect the transcriptome to provide an essential resource to investigate the molecular basis of commercially relevant-significant traits in P. monodon and other shrimp species.

Acute toxicity, accumulation and sublethal effects of four neonicotinoids on juvenile Black Tiger Shrimp (Penaeus monodon)

Chemosphere ◽  
2021 ◽  
Vol 275 ◽  
pp. 129918
Author(s):  
Peter Butcherine ◽  
Brendan P. Kelaher ◽  
Matthew D. Taylor ◽  
Corinne Lawson ◽  
Kirsten Benkendorff
Keyword(s):  
Acute Toxicity ◽  
Sublethal Effects ◽  
Penaeus Monodon ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

scholarly journals Novel Allergen Discovery through Comprehensive De Novo Transcriptomic Analyses of Five Shrimp Species

2020 ◽  
Vol 22 (1) ◽  
pp. 32
Author(s):  
Shaymaviswanathan Karnaneedi ◽  
Roger Huerlimann ◽  
Elecia B. Johnston ◽  
Roni Nugraha ◽  
Thimo Ruethers ◽  
...  
Keyword(s):  
De Novo ◽  
Penaeus Monodon ◽  
Clinical Importance ◽  
Major Allergen ◽  
Cross Reactivity ◽  
Multiple Sequence ◽  
Alpha Tubulin ◽  
Shrimp Species ◽  
Allergen Sources ◽  
Shellfish Allergy

Shellfish allergy affects 2% of the world’s population and persists for life in most patients. The diagnosis of shellfish allergy, in particular shrimp, is challenging due to the similarity of allergenic proteins from other invertebrates. Despite the clinical importance of immunological cross-reactivity among shellfish species and between allergenic invertebrates such as dust mites, the underlying molecular basis is not well understood. Here we mine the complete transcriptome of five frequently consumed shrimp species to identify and compare allergens with all known allergen sources. The transcriptomes were assembled de novo, using Trinity, from raw RNA-Seq data of the whiteleg shrimp (Litopenaeus vannamei), black tiger shrimp (Penaeus monodon), banana shrimp (Fenneropenaeus merguiensis), king shrimp (Melicertus latisulcatus), and endeavour shrimp (Metapenaeus endeavouri). BLAST searching using the two major allergen databases, WHO/IUIS Allergen Nomenclature and AllergenOnline, successfully identified all seven known crustacean allergens. The analyses revealed up to 39 unreported allergens in the different shrimp species, including heat shock protein (HSP), alpha-tubulin, chymotrypsin, cyclophilin, beta-enolase, aldolase A, and glyceraldehyde-3-phosphate dehydrogenase (G3PD). Multiple sequence alignment (Clustal Omega) demonstrated high homology with allergens from other invertebrates including mites and cockroaches. This first transcriptomic analyses of allergens in a major food source provides a valuable resource for investigating shellfish allergens, comparing invertebrate allergens and future development of improved diagnostics for food allergy.

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