scholarly journals ANALISIS ANTIBAKTERI EKSTRAK ETANOL SIPUT GONGGONG (Strombus canarium) TERHADAP BAKTERI PATOGEN

2016 ◽  
Vol 18 (2) ◽  
pp. 83
Author(s):  
Dessy Yoswaty ◽  
Zulkifli Zulkifli
Keyword(s):  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Average Length ◽  
Good Condition ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Marine Aquaculture ◽  
Inhibitory Region ◽  
The Difference

The study aims to analyze the use of antibacterial ethanol extract of gonggong snail (Strombus canarium) against pathogenic bacteria and analyzing bioactive compounds in gonggong snails for the development of marine aquaculture. The research method is an experimental method, performed in vitro, using a completelyrandomized design (CRD) with three replications. The subjects of research that pathogenic bacteria (Vibrio sp., C. perfringens, and Aeromonas sp.). The object of research is D1 gonggong snail extract 12.5%; D2 gongong snail extract 25%; D3 gonggong snail extract 50%; C4 gonggong snail extract 100%, ethanol D5 and D6 amosiklav discs (antibiotic chloramphenicol). Phytochemical screening test that alkaloids, saponins, flavonoids, steroids, and triterpenoids) from the ethanol extract of the gonggong snail. Data were analyzed by ANOVA test to determine the significance of the difference in diameter between the various treatments inhibitory region. The results showed that as many as 20 of the gonggong snail with morphometric body length size range of between 43.9 to 56.0 mm, the average length of 52.74 mm and a total weight of 16.21 grams on average. Results of research ethanol extract of gonggong snail is treated discs amoxiclav have inhibition (clear zone) highs against the bacterium Vibrio sp. (diameter 1.80 to 12.40 mm), A. hydrophila (diameter 4.78 to 21.37 mm) and C. perfringens (diameter 1.50 to 6.80 mm). Class of bioactive compounds of alkaloids and saponins identified in the ethanol extract of gonggong snail and class of bioactive compounds of flavanoid, steroids, and triterpenoids is not identified in the ethanol extract of gonggong snails. The results of the measurement of environmental quality parameters in waters around the coast of Bintan Island Village Senggarang still in good condition and has not been contaminated. This enables to support life gonggong snail (S. canarium). The ethanol extract of gonggong snails has the antibacterial activity that can be used to overcome the attacks of pathogenic bacteria in the development of marine aquaculture.

scholarly journals PERBEDAAN ZONA HAMBAT PERTUMBUHAN Staphylococcus aureus PADA BERBAGAI KONSENTRASI EKSTRAK ETANOL DAUN BIDURI SECARA IN VITRO

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Nyoman Mastra
Keyword(s):  
Staphylococcus Aureus ◽  
Growth Inhibition ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Growth Inhibition Zone ◽  
The Difference

ABSTRACTBackground Staphylococcus aureus is a pathogenic bacteria that can cause infection. Biduri leaf has antibacterial compound such as tannin, flavonoid, saponin, and polyphenol.Objective This study aimed to know the difference of growth inhibition zone of Staphylococcus aureus at the various concentration of ethanol extract of biduri leaf and to determine an effective concentration.Methods This study was the true experiment with posttest only control design, used Kirby-Bauer disk diffusion method with five concentrations (20%, 40%, 60%, 80%,100%), positive control (chloramphenicol 30 µg) and negative control (ethanol 96%).Result The result of this study showed the average of inhibition zone diameter of each concentration consecutively 26,2 mm, 28,3 mm, 29,7 mm, 31 mm and 31,5 mm. One Way Anova statistic analysis showed that the value of p is 0.000 so there is the difference of growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of a biduri leaf.Conclusion The conclusion of this study is there are differences in growth inhibition zone of Staphylococcus aureus at various concentrations of ethanol extract of biduri leaf and an effective concentration that can inhibit of Staphylococcus aureus is concentration 20% with mean inhibition zone is 26,2 mm. Keywords: ethanol extract of biduri leaf; Staphylococcus aureus; inhibition zone

EKSTRAK KULIT BUAH MANGGIS (Garcinia Mangostana L.) SEBAGAI OBAT ANTI DIARE

2019 ◽  
Vol 2 (1) ◽  
pp. 9-13
Author(s):  
Zaim Anshari ◽  
Chrismis Novalinda Ginting ◽  
Linda Chiuman ◽  
Yuliani Mardiati Lubis
Keyword(s):  
Blood Vessels ◽  
Smooth Muscles ◽  
Latin Square ◽  
Ethanol Extract ◽  
Garcinia Mangostana ◽  
Pharmacology And Toxicology ◽  
The Difference ◽  
North Sumatra ◽  
Papaverine Hydrochloride

This study aims to determine whether mangosteen rind extract (in the form of ethanol extract/EE) can be used as an anti-diarrhea drug after compared with other anti-diarrhea substances in three experimental groups. This research is an in vitro experimental study using adult male guinea pigs weighing 400-600 gr through the standard method of Magnus with the Latin square controlled experiment design. The study was conducted at the Pharmacology and Toxicology Laboratory of the Faculty of Pharmacy, University of North Sumatra. The results showed that the contraction of ileum in Ach with Atp + Ach compared the difference in contraction of ileum Ach with EE + Ach showed the difference in difference between the two contractions of the ileum was significant, the contraction of ileum in His with Dip + His compared indifference in contraction of ileum His with EE + His showed a difference indifference. the two ileal contractions are significant, the ileal contraction in the bar with Papa + Bar compared to the difference between the ileum bar contraction with EE + Bar shows no difference in the difference between the two ileum contractions. The conclusion is that the Mangosteen Skin Ethanol Extract works similarly to Papaverine Hydrochloride which is an antidiarrheal drug used to relax smooth muscles so that it can also make blood vessels dilate by relaxing smooth muscles in the walls of blood vessels.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals In Vitro and In Vivo Antibacterial Activity ofPunica granatumPeel Ethanol Extract againstSalmonella

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Jang-Gi Choi ◽  
Ok-Hwa Kang ◽  
Young-Seob Lee ◽  
Hee-Sung Chae ◽  
You-Chang Oh ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Clinical Signs ◽  
Ethanol Extract ◽  
Punica Granatum ◽  
Minimal Inhibitory Concentrations ◽  
Etoh Extract ◽  
Histological Damage

Punica granatumis commonly used in Korea as a traditional medicine for the treatment of pathogenic bacteria. In this study, we investigated thein vitroandin vivoantimicrobial activity ofP. granatumpeel EtOH extract (PGPE) against 16 strains ofSalmonella. The minimal inhibitory concentrations of PGPE were in the range of 62.5–1000 x03BCg mL-1. In addition, thein vivoantibacterial activity of the PGPE extract was examined in aS. typhimuriuminfection mouse model. Mice were initially infected withS. typhimuriumand then with PGPE. The extract was found to have significant effects on mortality and the numbers of viableS. typhimuriumrecovered from feces. Although clinical signs and histological damage were rarely observed in the treated mice, the untreated controls showed signs of lethargy and histological damage in the liver and spleen. Taken together, the results of this study indicate that PGPE has the potential to provide an effective treatment for salmonellosis.

scholarly journals In Vitro Determination of SunProtective Factor (SPF) of Dadap Serep (Erythrina Subumbrans (Haks.) Merr.) Leaf Extract Using Spectrophotometric Method

2019 ◽  
Vol 1 (1) ◽  
pp. 64-67
Author(s):  
Indri Maharini
Keyword(s):  
Bioactive Compounds ◽  
Spectrophotometric Method ◽  
Leaf Extract ◽  
Protective Factor ◽  
Ethanol Extract ◽  
Flavonoid Compounds

Dadap serep (Erythrina Subumbrans Haks Merr) contains bioactive compounds of flavonoids, saponins, isoflavones, alkaloids, and lectins. Flavonoid compounds have a sunscreen effect. This studyaimed to determinethe value of Sun Protective Factor (SPF) using spectrophotometry method with variations in the extract concentrations, such as500 ppm,750 ppm, and 1000 ppm.The results showed that the SPF values of each concentration were11.47 ±0.583335, 17.05 ± 0.898838,21.93 ± 0.791557, respectively. In conclusion, the ethanol extract of dadap serep hastheability to become sunscreen in the maximal and ultra category.

scholarly journals Evaluation of Anticancer Bioactive Compounds and Cytotoxicity of Kaffir Lime (Citrus hystrix Dc.) Callus Extract Post Preservation

2021 ◽  
pp. 179-192
Author(s):  
Woro Anindito Sri Tunjung ◽  
Sudewi Fajarina ◽  
Beni Hendro Prabowo ◽  
Frisca Damayanti ◽  
Asti Widyasari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Bioactive Compounds ◽  
Raw Material ◽  
Stress Factors ◽  
Alginate Encapsulation ◽  
Mtt Method ◽  
Preservation Method ◽  
The Difference

Previous studies have shown that kaffir lime extract is toxic to breast cancer T47D cells. To increase the bioactive compound's production for traditional cancer medicine raw material, we induce kaffir lime callus in vitro. One strategy to continuously produce kaffir lime callus is by using callus preservation. Our preliminary study used two preservation methods of callus, which are stored in 4 0C with or without alginate encapsulation. However, low temperatures and alginate encapsulation can be stress factors for plants, affecting the production of bioactive compounds and their anticancer ability. This study's objective was to determine the bioactive compounds of callus with and without preservation and their effect on the cytotoxicity against cancer cells. GC-MS detected the bioactive compounds in the extract, and cytotoxicity of callus against breast cancer (T47D), and non-cancer cell (Vero) is tested using MTT method. The results showed that preservation in 4 0C with and without encapsulation caused a change in bioactive compounds' profile. The change mainly precursor compound in callus, after being preserved and recultured, then changes into intermediates or final compounds, indicating the difference in growth phases. The terpenoid compounds detected after preservation are squalene and geranyl linalool. However, Kaffir lime callus extract pre and post preservation are not cytotoxic to both cancer and non-cancer cells. Therefore, the preservation method did not act as an elicitor to callus.

scholarly journals Extraction and Bioactivity Analysis of Major Flavones Compounds fromScutellaria baicalensisUsing In Vitro Assay and Online Screening HPLC-ABTS System

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Kwang Jin Lee ◽  
Pil Mun Jung ◽  
You-Chang Oh ◽  
Na-Young Song ◽  
Taesoo Kim ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Free Radical Scavengers ◽  
Vitro Assay ◽  
Abts Assay ◽  
Inflammatory Mediator Production ◽  
Rapid Characterization

The extraction efficiency of a number of solvent compositions for the improvement of bioactive compounds yield fromS. baicalensishas been investigated. Also, free radical scavengers in the glycoside baicalin (BG), wogonoside (WG), aglycon baicalein (B), and wogonin (W) compounds ofS. baicalensiswere screened, identified, and quantified using coupled offline ABTS and online screening HPLC-ABTS assay. Increasing ethanol content fractions resulted in decreased extract yield of bioactive compounds. In this case, the best yield of 37.01 mg/g in BG, WG, B, and W compounds was obtained by a dipping method with an extraction time of 4 h. In addition, the yield (43.05%) and IC50(34.04 μg/mL) determined through ABTS assay of the 60% aqueous ethanol extract were the most satisfactory of all solvent solutions tested. This result shows that an online screening HPLC-ABTS assay can be a powerful technique for the rapid characterization of bioactivity compounds in plant extracts. Moreover, their anti-inflammatory activities were evaluated via analyzed inhibitory effect on NO and inflammatory cytokine production. Furthermore, WG and W exhibited the strong inhibitory effects on inflammatory mediator production including NO, IL-6, and IL-1βin LPS-stimulated RAW 264.7 macrophages.

scholarly journals Evaluation of Bioactive Compounds, in Vitro Antioxidant Activity and Acute Toxicity of Ethanol Extract of Morinda lucida Leaves

2021 ◽  
pp. 32-38
Author(s):  
F. M. Idih ◽  
O. V. Alagbe ◽  
V. D. Sheneni ◽  
J. Ebune
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Lethal Dose ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Medium Size ◽  
Traditional Treatment ◽  
In Vitro Antioxidant Activity

Plants are known to contain phytochemicals of pharmacological relevance and as such have been utilized in the treatment and management of various diseases. Morinda lucida, a medium size tropical tree belonging to the rubiaceae family and widely distributed in Africa is one of these plants. It has been reportedly used in the traditional treatment and management of diseases. This study is aimed at identifying compounds with pharmacological relevance in the ethanol extract of Morinda lucida leaves, the antioxidant activity and lethal dose determination of the extract. The leaves of Morinda lucida was extracted with ethanol; phytochemical and bioactive compounds analysis, in vitro antioxidant activity and lethal dose (LD50) determinations were carried out. It was observed in the study that the extract contains alkaloids, quinines, quinones, flavonoids and tannins. The gas chromatography mass spectrometry (GC-MS) identified phenol 2, 4-bis (1,1-dimethylethyl) (2.82%), Stilbenes (12.32%), Phenoxazine (2.60%) and Benz(cd) indol-2(1H)-one, 1-methyl- (2.60%) amongst other compounds in the extract. The in vitro antioxidant activity evaluation of the extract revealed that it possesses a significant antioxidant activity which increased with increasing concentration. The LD50 determination revealed the extract was safe as there was no death recorded even at a dose as high as 5000 mg/kg. This study shows that Morinda lucida possesses enormous pharmacological potentials.

Inhibitory effect of Torilis anthriscus on growth of microorganisms

2009 ◽  
Vol 4 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Olgica Stefanovic ◽  
Dragana Stanojevic ◽  
Ljiljana Comic
Keyword(s):  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Bacterial Species ◽  
Antimicrobial Properties ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Dilution Method ◽  
Antibacterial And Antifungal Activities

AbstractAntibacterial and antifungal activities of aqueous, ethanol and ethyl acetate extract of Torilis anthriscus (L.) Gmel. (Apiaceae) were tested in vitro against ten species of bacteria and five of fungi. Antimicrobial properties were determined by disk diffusion and broth tube dilution method. In the minimum inhibitory concentrations (MICs), the ethanol extract showed the highest activity, followed by the ethyl acetate extract and the aqueous extract against bacterial species, while the extracts were inactive against the tested fungi species. The most active extract was chosen to examine the effects of its combinations with commercial antibiotics by checkerboard method. The obtained results showed that the interactions between ethanol extract/streptomycin and ethanol extract/chloramphenicol were additive and indifferent against the tested human-pathogenic bacteria. Synergism and antagonism were not observed.

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