scholarly journals Compatibility Between Beauveria bassiana (Bals.) and Neem Extract against Brown Plant-Hopper (Nilaparvata lugens Stal.)

2019 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Nova Laili Wisuda ◽  
Tri Harjaka ◽  
Indah Dwi Prasetyaningrum
Keyword(s):  
Beauveria Bassiana ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Spore Density ◽  
Neem Extract ◽  
Randomized Design ◽  
Speed Up ◽  
Nilaparvata Lugens Stål ◽  
And Control

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.

scholarly journals Keragaan Sumber Kitin untuk Mempertahankan Virulensi Beauveria bassiana (Bals.), Jamur Pengendali Wereng Batang Cokelat (Nilaparvata lugens Stal.)

2018 ◽  
Vol 22 (2) ◽  
pp. 143
Author(s):  
Nova Laili Wisuda ◽  
Subur Sedjati
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Potato Dextrose Agar ◽  
Spore Density ◽  
Colony Forming Unit ◽  
Randomized Design ◽  
Significant Difference ◽  
Lethal Time ◽  
Rice Medium ◽  
Nilaparvata Lugens Stål

Beauvaria bassiana is an entomopathogenic insect that effectively controls brown planthopper (BPH), but will decrease it virulences if nutrients containing chitin and protein is not added. The aim of this research is to find the best method of B. bassiana propagation with the addition of shrimp and cricket flour, both are chitin sources. Isolates were cultured with Potato Dextrose Agar (PDA) medium and continued with rice medium. This research method used non-factorial Completely Randomized Design (CRD) with three treatments consisting of control, addition of shrimp flour and addition of cricket flour per treatment each with six replications. The research parameters consist of percentage of mortality, percentage of  conidia rising time, and LT50 test. The chitin sources could increases the growth rate from 0.69 cm per day to 1.449 cm per day and increases the spore density by 4.8×106 CFU (Colony Forming Unit) up to 8.2×106 CFU. Chitin also affects the virulence of B. bassiana in BPH where it can increase the percentage of death starting from 3%until10% and made lethal-time of BPH faster from 0.25 to 0.45 day. There is no significant difference between the sources of chitin between shrimp and cricket flour, so it is more advisable to use cricket flour because it is less expensive. IntisariBeauvaria bassiana merupakan serangga entomopatogen yang efektif mengendalikan wereng batang cokelat (WBC), namun akan mengalami penurunan virulensi bila tidak diberikan nutrisi yang mengandung khitin dan protein. Penelitian ini bertujuan menemukan metode terbaik perbanyakan B. bassiana yang dilakukan dengan penambahan tepung ebi dan penambahan tepung jangkrik yang merupakan sumber kitin. Isolat dibiakkan dengan medium per- banyakan Potato Dextrose Agar (PDA) dan diteruskan hingga perbanyakan beras. Metode penelitian ini menggunakan Rancangan Acak Lengkap (RAL) non-faktorial dengan 4 perlakuan dan 6 ulangan yang terdiri atas kontrol, tanpa tambahan kitin, penambahan tepung ebi dan penambahan tepung jangkrik. Parameter penelitian terdiri persentase mortalitas, persentase waktu munculnya konidia dan uji LT50. Pemberian sumber kitin meningkatkan kecepatan pertumbuhan hingga 0,69−1,49 cm per hari dan meningkatkan kerapatan spora berkisar 4,8×106−8,2×106 CFU (colony forming unit). Kitin juga berpengaruh terhadap virulensi B. bassiana pada WBC dimana mampu meningkatkan persentase kematian 3−10% dan waktu paruh kematian WBC 0,25−0,45 hari lebih cepat. Tidak ada perbedaan yang berarti antara sumber khitin dari tepung jangkrik dan tepung ebi sehingga lebih disarankan untuk menggunakan tepung jangkrik karena lebih murah dan mudah didapat.

scholarly journals Menguji Tingkat Serangan Wereng Batang Coklat Nilaparvata lugens Stal (Hemiptera: Delphacidae) pada Varietas Padi Asal Pasaman di Rumah Kaca

2021 ◽  
Vol 5 (1) ◽  
pp. 55
Author(s):  
Eva Zulaikha ◽  
Arneti Arneti ◽  
Munzir Busniah
Keyword(s):  
Attack Rate ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Crop Failure ◽  
Rice Varieties ◽  
Randomized Design ◽  
Major Pest ◽  
Completely Randomized Design ◽  
Nilaparvata Lugens Stål ◽  
And Control

Brown planthopper (BPH) is a major pest on rice plants that can cause hopperburn and crop failure. The study aimed to determine the level of BPH attack on several local rice varieties in the Pasaman Regency. This research was conducted in a greenhouse, Faculty of Agriculture, Universitas Andalas, using a completely randomized design (CRD), with five treatments and five replications. The treatments were four different rice varieties from Pasaman and control (Siganteng, Pulau Batu, Mundam Kuning, Mundam Putih, IR 42). BPH was infested when the rice was five days after sowing. Observation parameters were the percentage and the intensity of the attacks. Observations were done until 15 days after infestation; after the IR 42 variety died 90%. The results showed that the attack rate of BPH on rice varieties from Pasaman was moderate. The lowest percentage of attack occurred in the Mundam Putih variety (94%), while the intensity of the attack was not significantly different from other varieties.

The toxic effects of neem extract and azadirachtin on the brown planthopper, Nilaparvata lugens (Stål) (BPH) (Homoptera: Delphacidae)

Chemosphere ◽  
2007 ◽  
Vol 67 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Sengottayan Senthil Nathan ◽  
Man Young Choi ◽  
Chae Hoon Paik ◽  
Hong Yul Seo ◽  
Jae Duk Kim ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Toxic Effects ◽  
Neem Extract ◽  
Nilaparvata Lugens Stål

scholarly journals PATOGENISITAS Beauveria bassiana STRAIN STGD 7(14)2 DAN STGD 5(14)2 TERHADAP WERENG COKLAT (Nilaparvata lugens STÅL)

2018 ◽  
Vol 11 (2) ◽  
pp. 122-132
Author(s):  
Yadi Suryadi ◽  
Wartono Wartono ◽  
Dwi Ningsih Susilowati ◽  
Puji Lestari ◽  
Cyntia Nirmalasari ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Internal Transcribed Spacer ◽  
Insect Pests ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Its Rdna ◽  
Stink Bug ◽  
West Java ◽  
Nilaparvata Lugens Stål

AbstrakBeauveria spp. merupakan salah satu jamur entomopatogen yang dapat digunakan sebagai agen biokontrol terhadap berbagai serangga hama. Tujuan penelitian ini adalah untuk menguji patogenitas Beauveria spp. terhadap serangga wereng coklat (Nilaparvata lugens Stål). Isolat-isolat Beauveria spp. diisolasi dari serangga walang sangit dari Situ Gede, Bogor, Jawa Barat. Identifikasi jamur dilakukan berdasarkan analisis data sekuen dari daerah Internal Transcribed Spacer (ITS) rDNA. Hasil uji menunjukkan bahwa patogenisitas isolat Stgd 5(14)2 dan Stgd 7(14)2 menghasilkan tingkat mortalitas 100% terhadap N. lugens. Isolat Stgd 5(14)2 dan Stgd 7(14)2 memiliki nilai LT50 yang rendah, danmenyebabkan kematian yang cepat terhadap wereng coklat. Hasil analisis filogenetik menunjukkan bahwa sekuen Stgd 5(14)2 dan Stgd 7(14)2 termasuk ke dalam spesies Beauveria bassiana s.str. Hasil studi ini merupakan kajian awal terhadap patogenisitas jamur entomopatogen B. bassiana terhadap wereng coklat, dan distribusinya di pertanaman padi Situ Gede, Jawa Barat.Abstract Beauveria spp. is one of the entomopathogenic fungi that can be used as biocontrol agents against various insect pests, including brown planthopper (Nilaparvata lugens Stal). This study aimed to test the pathogenicity of Beauveria spp. against N. lugens. Beauveria spp. were isolated from the rice stink bug insects, collected from Situ Gede, Bogor, West Java. Fungal identification was carried out based on the internal transcribed spacer (ITS) rDNA analysis. The pathogenicity assay revealed that Stgd 5(14)1 and Stgd 7(14)2 isolates were virulent against N. lugens, with a mortality of 100%. The LT50 (median lethal time) determination, indicated that Stgd 5(14)2  and Stgd 7(14)2 isolates had low value indicating a faster mortality in brown planthopper insects. Phylogenetic analysis showed that the sequences of Stgd 5(14)2 and Stgd 7(14)2 belong to Beauveria bassiana s.str. This is the preliminary pathogenicity trial of entomopathogenic fungi B. bassiana against brown planthopper and their distribution in rice-growing, Situ Gede area in West Java.

scholarly journals Phenotypic Screening of Elite Rice Germplasms against Brown Planthopper [Nilaparvata lugens (Stal.)] for Identification of Resistant Donor Lines

2019 ◽  
pp. 1-7
Author(s):  
M. Nikhil Raj ◽  
P. Rajani Kanth ◽  
N. R. G. Varma ◽  
M. Balram
Keyword(s):  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Research Centre ◽  
Randomized Design ◽  
Resistant Lines ◽  
Rice Genotypes ◽  
Underlying Mechanisms ◽  
Agriculture Research Institute ◽  
Nilaparvata Lugens Stål ◽  
Moderately Resistant

Aims: To identify Brown Planthopper (BPH) resistant rice genotypes and categorize all the test entries based on their level of resistance against BPH. Study Design: Completely Randomized Design. Place and Duration of Study: Poly-house, Department of Entomology, Rice Research Centre, Agriculture Research Institute (ARI), Rajendranagar, Hyderabad, India, between June 2016 and July 2017. Methodology: A total of 61 elite rice genotypes selected including resistant (PTB33) and susceptible check (TN1). All these test entries were screened against Brown Planthopper (BPH) using Standard Seedbox Screening Technique (SSST) inside poly-house conditions. Based on the Damage Score (DS) achieved during study, all entries were categorized into resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible (S) and highly susceptible (HS). Results: Among 61 cultures, the resistant check PTB33 and BM71 exhibited R reaction to BPH with a DS of 3.0, while twelve cultures viz. Milyang 63, IET 23993, HHZ 5 DT-1 DT-1, HHZ 25 SAL DT-1 DT-1, Bobhu Kongbu, BPT 2671, BPT 2611, MTU 1121, MTU 1001, MTU 1010, RNR 23079 and GSR 234 exhibited MR reaction to BPH with a DS ranging between 3.1 to 5.0. The rest of the cultures showed MS and S reactions while the susceptible check, TN1 along with other 12 cultures exhibited HS reaction to BPH with a DS of 9.0. Conclusion: Resistant and moderately resistant rice genotypes have been identified for development of BPH resistant lines. Further detailed studies are required to understand the underlying mechanisms of resistance among the R and MR genotypes.

scholarly journals Heat Stress Impedes Development and Lowers Fecundity of the Brown Planthopper Nilaparvata lugens (Stål)

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale
Keyword(s):  
Heat Stress ◽  
Brown Planthopper ◽  
Nilaparvata Lugens ◽  
Nilaparvata Lugens Stål

RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  
Keyword(s):  
Brown Planthopper ◽  
Transcript Level ◽  
Nilaparvata Lugens ◽  
The Body ◽  
Double Stranded Rna ◽  
Saccharopine Dehydrogenase ◽  
Body Weights ◽  
Nilaparvata Lugens Stål ◽  
Phloem Feeding ◽  
Wing Deformation

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.

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