The toxic effects of neem extract and azadirachtin on the brown planthopper, Nilaparvata lugens (Stål) (BPH) (Homoptera: Delphacidae)

Chemosphere ◽  
2007 ◽  
Vol 67 (1) ◽  
pp. 80-88 ◽  
Author(s):  
Sengottayan Senthil Nathan ◽  
Man Young Choi ◽  
Chae Hoon Paik ◽  
Hong Yul Seo ◽  
Jae Duk Kim ◽  
...  
2019 ◽  
Vol 4 (1) ◽  
pp. 9
Author(s):  
Nova Laili Wisuda ◽  
Tri Harjaka ◽  
Indah Dwi Prasetyaningrum

Beauveria bassiana fungi and neem extract as botanical insecticide can be combined to get the synergizing effect to control brown planthopper (BPH). This study was conducted to determine the best combination between the spore density of B. bassiana and concentration of neem extract to control BPH effectively. The two factorial treatment consisted concentration of B. bassiana (106  Colony Forming Unit (CFU) and 107 CFU) and concentration of neem extract (5%, 10%, 15%), and control.  The treatments were assigned in Completely Randomized Design with three replications. The data were analyzed using ANOVA and  LSD at 5%. The test was conducted in vitro and bio assay of testing insects, BPH, was conducted using dipping method.  B. bassiana was suitable with neem extract with T value ranged from 78.58- 90.38. The mortality of BPH occurred on day 5 after application. The highest mortality (91.67%) occurred on the treatment with B. bassiana 107 CFU + neem extract 10%. The shortest LT50 was found at the treatment of B. bassiana 106 CFU+  neem extract 15%  (2.74 days) but not significantly different from B. bassiana 107 CFU + neem extract 15% (2.76 days). B. bassiana spores combined with neem extract are assumed to speed up the mortality of BPH by increasing the concentration.


2010 ◽  
Vol 45 (3) ◽  
pp. 220-226 ◽  
Author(s):  
Jie Zhang ◽  
Liangxiong Hu ◽  
Shanfeng Ling ◽  
Jie Liu ◽  
Haidong Chen ◽  
...  

2013 ◽  
Vol 48 (1) ◽  
pp. 17-22 ◽  
Author(s):  
Jie Liu ◽  
Jie Zhang ◽  
Xiaowa Qin ◽  
Yong Chen ◽  
Fenghui Yuan ◽  
...  

2012 ◽  
Vol 47 (4) ◽  
pp. 297-308 ◽  
Author(s):  
Xiaowa Qin ◽  
Yong Chen ◽  
Jie Zhang ◽  
Jie Liu ◽  
Qi Liu ◽  
...  

PLoS ONE ◽  
2012 ◽  
Vol 7 (10) ◽  
pp. e47413 ◽  
Author(s):  
Jiranan Piyaphongkul ◽  
Jeremy Pritchard ◽  
Jeff Bale

2015 ◽  
Vol 105 (4) ◽  
pp. 390-398 ◽  
Author(s):  
P.-J. Wan ◽  
L. Yang ◽  
S.-Y. Yuan ◽  
Y.-H. Tang ◽  
Q. Fu ◽  
...  

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.


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