scholarly journals In Vitro Induced Resistance of Fusarium Wilt Disease (Fusarium oxysporum f.sp. cepae) by Salicylic Acid in Shallot CV ‘Bima Brebes’

2017 ◽  
Vol 2 (1) ◽  
pp. 001
Author(s):  
Khusnul Khotimah ◽  
Endang Sulistyaningsih ◽  
Arif Wibowo
Keyword(s):  
Salicylic Acid ◽  
Fusarium Oxysporum ◽  
Induced Resistance ◽  
Fusarium Wilt ◽  
Fusaric Acid ◽  
Wilt Disease ◽  
Resistance Response ◽  
Toxic Symptom ◽  
Fusarium Wilt Disease

Fusarium wilt caused by Fusarium oxysporum f.sp.cepae (Foc) is one of the most important diseases of onion in Indonesia. Induced resistance was one of the effective techniques to acquire resistance to fusarium wilt in shallot. Salicylic acid (SA) had been inferred to be in endogenous signal in the systemic acquired resistance response of plants. This research aimed to study the effect of exogenously added SA to resistance in shallot callus cv ‘Bima Brebes’ to fusarium wilt disease in vitro; and to determine the effective concentration of SA to induce resistance. A group of shallot callus was grown in MS medium containing varying SA concentration (0, 15, 20 and 25 ppm) in vitro for 2 weeks. Then, the callus were treated with toxin of Fusarium oxysporum, namely fusaric acid, to have observe the resistance response. In vitro selection was done twice in different fusaric acid concentration. Application of exogenous SA at all concentration did not suppres time of appearance of disease symptom yet. Toxic symptom in the callus was shown by the browning or blackening (off) of callus. Salicylic acid at concentration of 20 ppm effectively reduced the toxic symptom up to 16.66% and supported callus regeneration better than the concentration of 15 ppm and 25 ppm. The number of resistant callus regenerated was 66.67% at pretreatment of 20 ppm of SA.

scholarly journals Uji Ketahanan Planlet Anggrek Bulan (Phalaenopsis amabilis (L.) Bl.) Hasil Seleksi dengan Asam Salisilat Terhadap Fusarium oxysporum Secara In Vitro

2017 ◽  
Vol 17 (2) ◽  
Author(s):  
Ria Aulia Noviantia ◽  
Endang Nurcahyani ◽  
Martha Lulus Lande
Keyword(s):  
Salicylic Acid ◽  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
In Vitro Selection ◽  
Wilt Disease ◽  
Fusarium Wilt Disease ◽  
Selection For

Phalaenopsis amabilis cultivated have many constraints such as the appearance of fusarium wilt disease caused by Fusarium oxysporum. Plantlet P.amabilis which resistant to F. Oxysporum was selected in the solid Vacin and Went (VW) medium was added with salicylic acid at concentrations of 65 ppm, 75 ppm, and 85 ppm, compared with controls (0 ppm). The goals of the research were to study and determine of: 1) The SA concentration of planlet P. amabilis selection tolerant; 2) The proper concentration of AS during in vitro selection for suppressing the Fo. The research was carried out in December 2015 to February 2016 in the Botany Laboratory (in vitro research room), Departement of Biology, Faculty of MIPA, Lampung University. The result showed that: 1) The SA tolerant concentration for plantlet selection with P. amabilis was between 65 ppm - 85 ppm. 2) The 85 ppm of SA was effective for suppressing the Fo compared to 65 ppm and 75 ppm. Keywords: Phalaenopsis amabilis, salicylic acid, Fusarium oxysporum, In vitro, Resistance.

scholarly journals DETEKSI PENGIMBASAN KETAHANAN PISANG TERHADAP PENYAKIT LAYU FUSARIUM DENGAN ASAM FUSARAT (INDUCED RESISTANCE DETECTION OF BANANA AGAINST FUSARIUM WILT USING FUSARIC ACID)

2016 ◽  
Vol 19 (1) ◽  
pp. 40
Author(s):  
Christanti Sumardiyono ◽  
Suharyanto Suharyanto ◽  
Suryanti Suryanti ◽  
Putri Rositasari ◽  
Yufita Dwi Chinta
Keyword(s):  
Salicylic Acid ◽  
Phenolic Compounds ◽  
Fusarium Oxysporum ◽  
Induced Resistance ◽  
Cross Section ◽  
Fusarium Wilt ◽  
Fusaric Acid ◽  
Banana Plant ◽  
Water Stream ◽  
Soil Nutrition

Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is the most destructive disease of banana. Until today this disease has not been successfully controlled. Fusaric acid is a toxin produced by Foc. Tyloses produced inxylem that caused wilting and yellowing of banana plants, inhibit soil nutrition and water stream. The study carried out previously showed that enriched fusaric acid in banana culture induced the resistance of banana seedlings against Foc. The signal of induced resistance increased the phenolic compounds. One of the phenolic compounds is salicylic acid. The aim of this study was to detect induced resistance of banana plant from tissue cultured enriched with fusaric acid. The experiment was done in the field highly infected with Foc. Observation of resistance was done by measuring disease percentage of yellowing and wilting leaves.Tyloses produced in xylem was observed microscopically from cross section of root. Root damage intensity was counted using tyloses score. Salicylic acid content of root was analyzed with phenolic compounds method using HPLC. The results showed that banana plants from enriched tissues culture with 1.165 ppm of fusaric acid increased the resistance against Foc, but salicylic acid was not detected. Salicylic acid was only detected at low concentration (2 ppb) in moderate resistant banana roots from induced plants with 9.32 ppm of fusaric acid. The chromatogram showed three peaks of unknown phenolic compounds. Tyloses intensity was not related with induced resistance of banana against fusarium wilt. Advanced research is needed with more plants samples. It was suggested to identify the phenolic compounds which were detected in induced resistant plant.Keywords: banana, fusaric acid, fusarium wilt, induced resistance, salicylic acidLayu fusarium yang disebabkan oleh Fusarium oxysporum f. sp. cubense (Foc) adalah penyakit yang sangat merusak pada pisang dan belum dapat dikendalikan secara tuntas. Gejala berupa kelayuan daun karena tersumbatnya xilem karenapembentukan tilosis yaitu pertumbuhan sel dalam jaringan xilem. Pengimbasan ketahanan diharapkan dapat menjadi salah satu cara pengendalian penyakit layu fusarium. Penelitian sebelumnya menunjukkan penambahan asam fusaratdalam kultur jaringan dapat mengimbas ketahanan bibit pisang terhadap penyakit layu fusarium. Asam salisilat adalah salah satu signal ketahanan yang akan meningkat kandungannya bila terjadi peningkatan ketahanan akibat pengimbasan. Penelitian ini bertujuan untuk mendeteksi hasil pengimbasan ketahanan pisang dengan asam fusarat dalam kultur jaringan. Tanaman telah ditanam di lapangan yang terinfeksi berat oleh Foc. Intensitas penyakit di lapang diamati dengan menghitung persentase daun menguning dan atau layu. Intensitas kerusakan akar diamati dengan pembuatan irisan tipis dan pengamatan tilosis dengan cara skoring. Analisis asam salisilat dalam akar dilakukan dengan metode analisis senyawa fenol menggunakan HPLC. Hasil penelitian tanaman dari bibit yang diimbas dengan 1,165 ppm asam fusarat dalam kultur jaringan menunjukkan peningkatan ketahanan di lapang. Intensitas tilosis lebih rendah pada tanaman yang diimbas ketahanannya dibandingkan yang tidak diimbas. Asam salisilat dalam tanaman yang diimbas ketahannnya denganasam fusarat 9,32 ppm terdeteksi pada konsentrasi yang sangat rendah yaitu 2 ppb, dengan ketahanan moderat. Pada tanaman hasil pengimbasan yang menunjukkan kriteria tahan asam salisilat tidak terdeteksi, namun terdeteksi tigapuncak senyawa fenol yang belum teridentifikasi. Intensitas tilosis pada tanaman yang diimbas ketahanannya tidak menunjukkan penurunan dibandingkan dengan tanaman yang tidak diperlakukan. Penelitian ini perlu dilanjutkan dengan sampel yang lebih banyak. Identifikasi jenis senyawa fenol perlu dilakukan dalam penelitian lanjutan.Kata kunci: asam fusarat, asam salisilat, layu fusarium, pengimbasan ketahanan, pisang

scholarly journals EVALUASI KETAHANAN AKSESI KENAF (Hibiscus cannabinus L.) TERHADAP PENYAKIT LAYU Fusarium oxysporum SCHLECT

2020 ◽  
Vol 6 (2) ◽  
pp. 29
Author(s):  
GEMBONG DALMADIYO ◽  
CECE SUHARA ◽  
SUPRIYONO SUPRIYONO ◽  
SUDJINDRO SUDJINDRO
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Hibiscus Cannabinus ◽  
Resistant Variety ◽  
Fusarium Wilt Disease ◽  
Screen House

<p><strong>Evaluation on the resistance of kenaf accessions (Hibis¬ cus cannabinus /..) to Fusarium oxysporum Schlect</strong></p><p>Resistant variety is one of the most important components controlling of fusarium wilt disease on kenaf caused by Fusarium oxysporum Schlect. To ind out resistant variety an evaluation on kenaf accessions was conducted in the laboratory and screen house of Phytopathology, RITFC, Malang in June-December 1997. The results of the selection on 77 accessions showed that 41 accessions were highly resistant, 1 2 accessions were resistant, 7 accessions were moderate, 12 accessions were susceptible, and 5 accessions were highly susceptible Three resistant and highly resistant accessions were namely 85-9-73, DS/005 H, and FJ/004 He could inhibit F. oxysporum growth about 23.40- 32.43 mm and its discolorisation about 0.0-13.4%.</p>

scholarly journals Biological management of fusarium wilt on tomato caused by Fusarium oxysporum f. sp. lycospersici by some plant growth-promoting bacteria

2020 ◽  
Author(s):  
Halima Z. Hussein ◽  
Shaker I. Al-Dulaimi
Keyword(s):  
Fusarium Oxysporum ◽  
Disease Severity ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Systemic Resistance ◽  
Control Treatment ◽  
Plant Growth Promoting Bacteria ◽  
Tomato Plants ◽  
Fusarium Wilt Disease ◽  
Infection Percentage

AbstractChemical approaches have been applied to combat Fusarium wilt disease for a long time. Even though pesticides are effective in controlling the disease, they continue to damage the environment. Environmental-friendly approaches to manage plant disease are the goal of many studies recently. This study was conducted to assess the efficacy of some bio-agents in induction of systemic resistance in tomato plants as a management approach of Fusarium wilt disease caused by Fusarium oxysporum f.sp. lycopersici (FOL) under condition Plastic house. Results of the plastic house experiments showed that all treatments in decreased Fusarium disease percentage and severity on tomato, two bacterial combinations (Streptomyces sp. (St) and Pseudomonas fluorescence (Pf)) decreased the infection percentage and disease severity with 16.6% and 8.3%, respectively. Treatment with St reduced the infection percentage and disease severity with 33.3% and 22.8%, while the Pf treatment showed 41.6% and 31.2% reduction in infection percentage and disease severity, compared to 100% and 91.6% in the control treatment. Results of induced systemic resistance (ISR) biochemical indicators showed significant differences in tomato plants. Peroxidase and Phenylalanine-Ammonia-Lyase (PAL) activity and the Phenol content increased significantly 14 days after treatments compared to the control treatment, which contains only the fungal pathogen FOL.

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