scholarly journals Liquid Chromatography Mass Spectrometry (LC-MS) Fingerprint Combined with Chemometrics for Identification of Metabolites Content and Biological Activities of Curcuma aeruginosa

2018 ◽  
Vol 18 (1) ◽  
pp. 43 ◽  
Author(s):  
Dewi Anggraini Septaningsih ◽  
Latifah Kosim Darusman ◽  
Farit Mochamad Afendi ◽  
Rudi Heryanto
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Herbal Medicine ◽  
Brine Shrimp ◽  
Biological Activities ◽  
Partial Least Square ◽  
Least Square ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chemometric Method

Curcuma aeruginosa is known as one of the components of herbal medicine with various biological activities. This research aims to identify the metabolites content of C. aeruginosa related to their biological activities using LC-MS fingerprint combined with chemometrics. C. aeruginosa from 3 areas in Java were collected and macerated with ethanol and then analyzed with LC-MS. Along with this analysis, the antioxidant activity of all samples was determined using CUPRAC method, and the toxicity was determined using Brine Shrimp Lethality Test (BSLT), and chemometric method was used Principle Component Analysis (PCA) and Partial Least square (PLS). Metabolites profiles showed 175 predicted compounds, in which the dominant compounds are from the sesquiterpene of Curcuma genus. The PCA metabolites profiles can separate the samples by their location of origin. Interpretation of the correlation between metabolites profiles and their bioactivities was determined using PLS technique. The results showed that the toxicity of samples was exerted by compounds with ion mass of 312.28 and 248.15, which have the highest antioxidant and toxicity potentials. Compounds with ion mass of 248.15 were predicted to be 9-Oxo-neoprocurcumenol, 7α,11α,-Epoxy-5β-hydroxy-9-guaiaen-8-one, Curcumenolactone A, or Curcumenolactone B. While compound with ion mass of 312.28 was predicted to tetrahydro-bisdemethoxycurcumin.

scholarly journals COMPOSITION OF PURIFIED ANTHOCYANIN ISOLATED FROM TEAK AND IT’S IN VITRO ANTIOXIDANT ACTIVITY

2017 ◽  
Vol 9 (9) ◽  
pp. 258 ◽  
Author(s):  
Greeshma Murukan ◽  
Murugan K.
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
In Vitro Culture ◽  
Oxygen Radical ◽  
Sulphonic Acid ◽  
Oxygen Radical Absorbance Capacity ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Objective: The present study evaluates purification, characterization of anthocyanin from in vitro culture of teak and its antioxidant potential.Methods: Anthocyanin was extracted from in vitro culture, purified by using amber lite XAD column and fractionated by Liquid chromatography mass spectrometry (LC-MS/MS). Various antioxidant assays were carried such as 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), Oxygen radical absorbance capacity (ORAC), Nitric oxide (NO) and Hydrogen peroxide (H2O2).Results: Liquid chromatography mass spectrometry (LC-MS/MS) revealed the major fraction as cyanidin 3-(2-xylosyl-rutinoside) with unknown peaks. The amount of anthocyanin was 15.23 mg/g monomeric anthocyanin. Further, the potential antioxidant capacity of the teak anthocyanin was comparable to common vegetables and fruits. Similarly, high correlations of anthocyanin with antioxidant activity, such as oxygen radical absorbance capacity (ORAC), 2,2'-azino-bis-3-ethyl-benzothiazoline-6-sulphonic acid (ABTS), and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) (r = 0.95, 0.93, and 0.80) were found.Conclusion: The high anthocyanins content and potential antioxidant activity suggests that teak anthocyanin may be applied in the food industry as a good source of natural pigments

Rosa rubiginosa and Fraxinus oxycarpa herbal teas: characterization of phytochemical profiles by liquid chromatography-mass spectrometry, and evaluation of the antioxidant activity

2017 ◽  
Vol 41 (15) ◽  
pp. 7681-7688 ◽  
Author(s):  
J. Jiménez-López ◽  
A. Ruiz-Medina ◽  
P. Ortega-Barrales ◽  
E. J. Llorent-Martínez
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Folk Medicine ◽  
Liquid Chromatography Mass Spectrometry ◽  
Rosa Rubiginosa ◽  
Chromatography Mass Spectrometry ◽  
Phytochemical Profiles

The consumption of infusions and herbal teas has always been an important part of folk medicine.

scholarly journals Chemical Analysis of the Main Metabolites of Three Spontaneous Plants From the Saharan Area (Algeria)

2021 ◽  
Author(s):  
Imène RADJAI
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Analysis ◽  
Biological Activities ◽  
Time Of Flight ◽  
Chemical Study ◽  
Biological Functions ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Cotula Cinerea

Spontaneous plants metabolites are more widespread for their properties and biological functions. Also, natural products have reminded diverse scientists to take a delight in their medical and insecticidal applications linked to the environmental. A variety of metabolites have a defensive function for the plants. Thus, three spontaneous plants: Caroxylon imbricatum, Tetraena alba and Cotula cinerea collected from two ecotypes and analyzed by two known conventional methods:Gas Chromatography‐Mass Spectrometry GC QTOF(quadrupole time of flight )_MS and Liquid Chromatography-Mass spectrometry LCQTOF(quadrupole time of flight )_MS. The investigation conducted out on the identification and quantification of metabolites revealed the main metabolites which have biological activities as a part of an alternative to synthetic insecticides. The chemical study showed the presence of N-Butylbenzensulfonamide and Sulfoxycaprylicacid in the three plants. N-Carboxy-methionineresidue, Butanoicacid and Valine were found in those of Cotula cinerea and Caroxylon imbricatum (Forssk.). Artomunoxanthentrione, Glycoaldehyde, Indoline, ,Benzensulfonamide and Oxoproline were detected in extracts of Caroxylon imbricatum (Forssk.) and Tetraena alba (L.f.) In addition, Pyrroline is the only compound common in Cotula cinerea and Tetraena alba (L.f.).

scholarly journals AKTIVITAS ANTIOKSIDAN DAN IDENTIFIKASI SENYAWA EKSTRAK JAMUR LINGZHI (Ganoderma lucidum) DENGAN LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY (LC-MS)

EKOLOGIA ◽  
2019 ◽  
Vol 19 (2) ◽  
pp. 65-72
Author(s):  
Farida Nuraeni ◽  
Septi Bernadetha Br Sembiring
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Time Variation ◽  
Ganoderma Lucidum ◽  
Water Extract ◽  
Ethanol Extract ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Dpph Method

Lingzhi mushroom is widely used as an alternative treatment to reduce blood pressure and blood sugar levels, to maximize the potential of the Lingzhi fungus antioxidant tests are carried out. This study aims to determine the potential antioxidant activity of water extract and 70% ethanol extract of Lingzhi mushroom with maceration extraction time variation and identification of compounds with Liquid Chromatography Mass Spectrometry (LC-MS). This research begins with the determination of fresh lingzhi mushrooms then made simplicia and extracted by maceration with time variation with soaking time of 24 hours, 48 hours and 72 hours each with 2 solvents namely water and 70% ethanol. The extract was tested by phytochemistry followed by testing the antioxidant activity of Lingzhi mushroom extract (ganoderma lucidum) by DPPH method. Then the compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS). Based on the results of research that maceration for 1 hour (1 day) Lingzhi mushroom with 70% ethanol extraction has the potential as an antioxidant with an IC50 value of 94.83 ppm. The results of identification with LC-MS in 70% ethanol extract as a compound that has potential as an antioxidant are Bisphenol M compounds, and 1- - [[2- (3,4-Dimethoxyphenyl) amino]} -3-methyl-2-octylpyrido [1 , 2-a] benzimidazole-4-carbonitrile.

scholarly journals Metabolomics of Ginger Based on Ultra-Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry Technology

2020 ◽  
Author(s):  
Hui Yan ◽  
Dongqian Zou ◽  
Guisheng Zhou ◽  
Hanwen Yu ◽  
Penghui Li ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Quality Evaluation ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Partial Least Square ◽  
Least Square ◽  
Related Factors ◽  
Flight Mass Spectrometry ◽  
Mass Spectrometry Technology

Abstract Objectives Dried ginger and ginger are the same type of medicine and food. The differential components of ginger and dried ginger, dried ginger and ginger charcoal were investigated. Materials and Methods The experimental materials were divided into three sample groups: the ginger group, dried ginger group and ginger charcoal group. The ginger group, dried ginger group and ginger charcoal group were qualitatively analyzed by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS). The data were processed by Marker View Software. Principal component analysis (PCA) and orthogonal partial least-square discriminant analysis (OPLS-DA) were performed with SIMCA 13.0 Software. The differential components of the ginger group and dried ginger group as well as the dried ginger group and ginger charcoal group with a VIP>2 (p<0.05), were identified with PeakView 1.2 Software. Results Ten differential components, including 6-gingerol, 8-gingerol and 10-gingerol, were identified between the ginger group and dried ginger group; 13 differential components, including 6-shogaol, 10-gingerol and zingiberone, were identified between the dried ginger group and ginger charcoal group. Conclusions The main differential components between the ginger group and dried ginger group, dried ginger group and ginger charcoal group were gingerols and diphenylheptanes. Based on metabolomics analysis of the chemical composition of ginger medicinal materials, effects and other related factors, it is recommended that 6-gingerol, 6-shogaol and zingiberone should be used as indicative components for the respective quality evaluation of ginger, dried ginger and ginger charcoal. The results of this study may provide a basis for the reasonable quality evaluation of ginger medicinal materials.

Gut microbiota-mediated deglycosylation of ginsenoside Rb1 in rats: in vitro and in vivo insights from quantitative ultra-performance liquid chromatography-mass spectrometry analysis

2015 ◽  
Vol 7 (15) ◽  
pp. 6173-6181 ◽  
Author(s):  
Kang An ◽  
Zhang Shengjie ◽  
Shan Jinjun ◽  
Di Liuqing
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Biological Activities ◽  
Ultra Performance Liquid Chromatography ◽  
Mass Spectrometry Analysis ◽  
Ginsenoside Rb1 ◽  
Liquid Chromatography Mass Spectrometry ◽  
Spectrometry Analysis

Ginsenoside Rb1, an ingredient of the herbal medicine Panax ginseng, possesses a variety of biological activities.

Liquid chromatography-mass spectrometry untargeted metabolomics reveals increased levels of tryptophan indole metabolites in urine of metabolic syndrome patients

2020 ◽  
Vol 26 (6) ◽  
pp. 379-387
Author(s):  
Moises Guerrero Esperanza ◽  
Katarzyna Wrobel ◽  
Armando Gomez Ojeda ◽  
Ma Eugenia Garay-Sevilla ◽  
Alma Rosa Corrales Escobosa ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Metabolic Syndrome ◽  
Acetic Acid ◽  
Liquid Chromatography ◽  
Capillary Liquid Chromatography ◽  
Partial Least Square ◽  
Least Square ◽  
Untargeted Metabolomics ◽  
Control Group ◽  
Indole 3 Acetic Acid

Metabolic syndrome (MetS) is a multifactor condition predisposing for diabetes, cardiovascular diseases and other degenerative disorders. Although several diagnostic criteria have been established, none of them is specific and there is a call for better pathophysiological explanation of MetS and for the discovery of molecular biomarkers. Phenotype characterization at metabolome level might be useful for both purposes. To this end, our aim was to perform comparative untargeted metabolomics of urines from MetS patients and from the control group. The study participants included 52 diagnosticated and 50 healthy individuals from Leon city in central Mexico; 23 anthropometric and clinical parameters were measured and submitted to Principal Component Analysis (PCA). The obtained PCA model allowed us for selection of 11 MetS patients and 13 control subjects, correspondingly representative for each of the two groups (clearly separated in PCA). The first morning urines from these subjects were ambulatory collected and, after methanol extraction and acidification, were submitted to capillary liquid chromatography-high resolution mass spectrometry (LC-HRMS). The obtained data were analyzed on MetaboScape® platform (Bruker Daltonics). Specifically, t-test applied to LC-HRMS data revealed several ions presenting at least 3-fold higher intensities in MetS with respect to the control samples (p < 0.05). Data analysis and complementary experiments yielded the identification of the following metabolites: indole-3-acetic acid, indole-3-acetic acid-O-glucuronide, N-(indol-3-ylacetyl) glutamine, indole-3-carbaldehyde and hydroxyhexanoycarnitine. Additionally, indole-3-carboxylic acid was annotated with 2.13-fold higher abundance in MetS patients. To assess the contribution of individual metabolites in the difference between two groups of subjects, partial least square discriminant analysis was performed for LC-HRMS data and the obtained values of variable importance in projection (VIP), confirmed the association of six above mentioned compounds with MetS. Overall, this study provides direct evidence on the disturbed catabolism of tryptophan in metabolic syndrome.

scholarly journals PSXI-12 Comparative metabolomics of blood plasma from Hanwoo beef cattle at different ages and fed diets with different nutritional levels, by using liquid chromatography-mass spectrometry

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 406-407
Author(s):  
Jin Young Jeong ◽  
Minseok Kim ◽  
Kondreddy Eswar Reddy ◽  
Seul Lee ◽  
Soohyun Cho ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Beef Cattle ◽  
Blood Plasma ◽  
Metabolic Pathways ◽  
Partial Least Square ◽  
Feeding Strategies ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Different Ages

Abstract Blood metabolome can be used to estimate the growth, nutrition, and health status of livestock. The objective of this study is to find the differently expressed metabolome according to ages and nutritional levels in feeds to predict and control cattle performances. We used Hanwoo cattle at different ages that were fed diets with different nutritional levels. One hundred thirty two blood samples were collected from 22 Hanwoo steers at 13, 16, 19, 22, 25, 28 months and compared their blood metabolomes by using liquid chromatography mass spectrometry. The results of our comparative analysis showed clear discriminations in blood metabolomic profiles among the ages but not between nutritional levels. Based on the results of t-test, fold changes, and partial least square discriminant analysis, 19 metabolites showed high sensitivity for ages. Alanine, asparagine, aspartic acid, betaine, carnitine, choline, citrulline, creatine, cysteine, glutamine, glycine, histidine, lactate, leucine, proline, pyruvate, serine, tryptophan, and valine could be directly linked to ages. In particular, three metabolic pathways, ammonia recycling; urea cycle; and glycine and serine metabolism were shown to be enriched with the ages (FDR &lt; 0.05, P &lt; 0.05). Thus, the differently expressed metabolites and their related metabolic pathways in the blood plasma may contribute to the biomarkers which indicate the potential for early growing and fattening of indiviual beef cattle. Our findings may allow for better understanding of the mechanism of cattle growth physiology and metabolism, which is necessary for selecting appropriate feeding strategies to improve beef quality and productivity.

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