scholarly journals EVALUATION OF RE-USED HPTLC PLATE FOR QUALITATIVE AND QUANTITATIVE ANALYSIS

2010 ◽  
Vol 6 (3) ◽  
pp. 338-340
Author(s):  
Lestyo Wulandari
Keyword(s):  
Quantitative Analysis ◽  
Mobile Phase ◽  
Qualitative And Quantitative Analysis ◽  
Hptlc Plate ◽  
Qualitative And Quantitative ◽  
A Value ◽  
Better Than

The eficiency chromatography of HPTLC plate is better than conventional TLC plate but it more expensive. Due to the higher price of HPTLC plate compare to conventional TLC plate, the application of ";re-used"; HPTLC plate has been tested. After being used for analyzing samples, the plates were developed again with the mobile phase, then dried for 60 min before using for the next measurement. The qualitative chromatography profile showed that ";re-used"; HPTLC plate has less contaminant than new HPTLC plate and the RSD of the precision quantitative testing of the plates reused  three times showed a value > 2%.   Keywords: reused, HPTLC, TLC, quantitative, qualitative

scholarly journals Isolation of Arbutin from Leaves and Fruits of Buni (Antidesma Bunius L. Spreng) As Tyrosinase Enzym Inhibitor

2021 ◽  
Vol 4 (1) ◽  
pp. 8-18
Author(s):  
Gea Ocktiah Palensina ◽  
Leni Rosita ◽  
Zuraida Sagala
Keyword(s):  
Quantitative Analysis ◽  
Mobile Phase ◽  
Enzyme Inhibitor ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Extractive Substances ◽  
Ic50 Values ◽  
Rf Values ◽  
Tyrosinase Enzyme

Several studies have shown that plant extractive substances have the potential as active compounds inhibiting the enzyme tyrosinase. Arbutin is an enzyme inhibitor of tyrosinase, known as a popular whitening agent used in cosmetics because of its effectiveness in overcoming skin hyperpigmentation. The purpose of this study was to conduct a qualitative and quantitative analysis of arbutin on Buni Leaves and Fruits (Antidesma bunius L. Spreng). The raw simplicia used are mature and young buni leaves, green, red and purple buni fruits. The extraction method is maceration using methanol as solvent. The initial screening for arbutin content was carried out using thin layer chromatography (TLC) and dichlormethan:methanol 50:50 used as mobile phase. Isolation of arbutin content was carried out using Preparative TLC with the same eluent. Qualitative and quantitative analysis were performed using High Pressured Liquid Chromatography (HPLC) with mobile phase of acetonitrile: water 60:40. The tyrosinase enzyme inhibition activity test was then carried out in vitro using 96-well microplate, l-tyrosine and l-dopa were used as substrate at a wavelength of 492 nm. The Rf values obtained ??for mature buni leaves and green buni fruits, respectively 0.61 and 0,62. The retention time of HPLC chromatogram respectively 2,784 minutes and 2,758 minutes. Arbutin levels in leaves and fruits are 7.9 mg / g and 2 mg / g. The activity of the enzyme tyrosinase of mature buni leaves on L-dopa and L-tyrosine substrate were respectively stated as IC50 values ??of 88.7191ppm and 101.33347 ppm. The activity of the enzyme tyrosinase of the green buni fruit on L-dopa and L-tyrosine substrate respectively stated IC50 values ??of 198,0293 ppm and 246,1296 ppm.

MEMIKAT HATI WAJIB PAJAK SEHINGGA MEMILIKI WILLINGNESS TO COMPLY MELALUI PENYULUHAN PAJAK BERDASARKAN FOGG BEHAVIORAL MODEL

INFO ARTHA ◽  
2017 ◽  
Vol 2 ◽  
pp. 56-79
Author(s):  
NFN Khusnaini ◽  
Agung Widi Hatmoko
Keyword(s):  
Data Analysis ◽  
Quantitative Analysis ◽  
Statistical Data ◽  
Tax Compliance ◽  
Behavioral Model ◽  
Control Group ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Quasi Experimental ◽  
Experimental Process

Attitudes towards tax compliance (willingness to comply) Indonesian society is still low. Required an innovative tax dissemination to increase it. The purpose of this study was to determine whether the tax dissemination based on Fogg Behavioral Model (FBM) approach may increase wiliingness to comply. The FBM based tax dissemination asserts that for a person to perform a target behavior, which is a willingness to comply, he or she must be sufficiently motivated, have the ability to perform the behavior, and be trigerred, to perform the behavior. This research is a combination of qualitative and quantitative analysis of statistical data results of the questionnaire, interviews, and observations of the respondent and the experimental process of dissemination. The method used in this study is a quasi experimental with patterns of nonequivalent control group (pretest-post which is not equivalent). Based on the results of data analysis, interviews and observations of the respondent and the experiment, this research showed that theFBMbasedtaxdissemination hasapositiveimpactto willingnesstocomplyofthetaxpayers. 

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