scholarly journals ISOLATION OF ACTINOMYCIN D FROM MARINE DERIVED Streptomyces

2010 ◽  
Vol 7 (3) ◽  
pp. 350-353
Author(s):  
Abdullah Rasyid ◽  
Kyoko Adachi
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Marine Bacterium ◽  
Actinomycin D ◽  
Culture Supernatant ◽  
Culture Broth ◽  
Bacterial Cells ◽  
Antibacterial Screening ◽  
Activity Test ◽  
Bioactive Substance

The bioactive substance producing strain, a marine bacterium A5S-46 was found in the antibacterial screening. This strain was isolated from the artificial sponge set in the coastal seawater at Iriomote islands (Japan). The crude extract from culture broth of the strain A5S-46 was assayed to the antibacterial activity test against the seven kinds of bacterial test strains. The active substance accumulated in the both bacterial cells and culture supernatant. Based on the 1H NMR and the LC/PDA/MS data, the bioactive substance was identified to be actinomycin D.   Keywords: Marine bacteria, Actinomycin D, Antibacterial, Anticancer

scholarly journals Endophytic Fungi Isolated from Rhizophora mucronata and Their Antibacterial Activity

2013 ◽  
Vol 8 (2) ◽  
pp. 69 ◽  
Author(s):  
Kustiariyah Tarman ◽  
Dwi Safitri ◽  
Iriani Setyaningsih
Keyword(s):  
Antibacterial Activity ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Culture Broth ◽  
Rhizophora Mucronata ◽  
Economic Potential ◽  
Mangrove Plant ◽  
Traditional Medicines ◽  
Activity Test ◽  
Fungal Extracts

Mangrove plants are known as source of traditional medicines. Endophytic fungi have an important economic potential for antibacterial, enzyme and secondary metabolites that are useful especially in pharmaceutical industry. The purpose of this research was to determine the antibacterial activity of the endophytic fungal extracts from mangrove plant Rhizophora mucronata against diarrhea causing bacteria. This research included isolation, characterization, cultivation, extraction, and antibacterial activity test. The study yielded 5 types of endophytic fungi with different morphology. Endophytic fungus DS1 was the selected isolate based on antagonism test. The growth pattern showed the stationary phase of isolate DS1 was on the 15th day. Culture broth extract showed considerable inhibition on Pseudomonas aeruginosa with diameter of inhibition zone was 18.5 mm ± 3.32. Extract of mycelium inhibited the growth of Staphylococcus aureus (2 mm). The culture broth extract showed more potent antibacterial activity in comparison with the mycelium extract.

scholarly journals Antibacterial Potential Chloroform Extract of Photobacterium phosphoreum Bacteria Symbiotic in Light Organs of Squid Loligo duvauceli

2020 ◽  
Vol 4 (2) ◽  
pp. 60-67
Author(s):  
Iin Putriyani ◽  
Delianis Pringgenies ◽  
Ali Ridlo
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Crude Extract ◽  
Marine Invertebrates ◽  
Inhibition Zone ◽  
Chloroform Extract ◽  
Photobacterium Phosphoreum ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Activity Test

Microorganisms that live in association with marine invertebrates are able to produce a compound similar  to those produced by marine invertebrates and are thought to be potential as bioactive materials. Supplement  extracts from the Photobacterium phosphoreum bacterial culture have been shown to have antibacterial activity. The purpose of this study was to determine the anti-bacterial activity of the chloroform extract of Photobacterium  phosphoreum biomass against pathogenic bacteria Bacillus substilis, Staphylococcus aureus, Salmonella sp. and  Escherichia coli. The extraction process was carried out by the liquid-liquid extraction method. Fractionation was  performed using Open Column Chromatography (KKT). The antibacterial activity test was conducted by the agar diffusion method according to Kirby-Bauer. The results showed that for the antibacterial activity test of crude  extract, the largest inhibition zone diameter occurred in Escherichia coli with a concentration of 50 µg / disk at 24 h incubation, namely (9.80 ± 0.75 mm), while the smallest inhibition zone diameter occurred in Salmonella sp. by  giving a concentration of 10 µg / disk at an incubation time of 48 hours, namely (6.03 ± 0.05) mm. Of the 6  fractions resulting from crude extract fractionation, it was known that fraction 5 was the most active fraction and  inhibited the growth of the tested bacteria. The largest inhibition zone diameter occurred in Escherichia coli (9.83  ± 0.28) mm, while the smallest inhibition zone diameter occurred in Salmonella sp. (8.63 ± 0.20 mm)

scholarly journals In vitro activities of Acacia nilotica (L.) Delile bark fractions against Oral Bacteria, Glucosyltransferase and as antioxidant

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ali Mahmoud Muddathir ◽  
Ebtihal Abdalla M. Mohieldin ◽  
Tohru Mitsunaga
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Gallic Acid ◽  
Crude Extract ◽  
Inhibitory Activity ◽  
Oral Bacteria ◽  
Acacia Nilotica ◽  
Assay Method ◽  
Medicinal Uses ◽  
Wide Range

Abstract Background Dental caries and periodontal disease are the most common chronic infectious oral diseases in the world. Acacia nilotica was commonly known in Sudan as Garad or Sunt has a wide range of medicinal uses. In the present study, antibacterial activity of oral bacteria (Streptococcus sobrinus and Porphyromonas gingivalis), inhibitory activity against glucosyltransferase (GTF) enzyme and antioxidant activity were assayed for methanolic crude extract of A. nilotica bark and its fractions. Methods Methanoilc crude extract of A. nilotica bark was applied to a Sephadex LH-20 column and eluted with methanol, aqueous methanol, and finally aqueous acetone to obtain four fractions (Fr1- Fr4). Furthermore, the crude extract and fractions were subjected to analytical high performance liquid chromatography (HPLC). The crude extract and its fractions were assayed for antibacterial activity against S. sobrinus and P. gingivalis using a microplate dilution assay method to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), as well as GTF inhibition and antioxidant activity using ABTS radical scavenging method. Results Fractions (Fr1 and Fr2) exhibited MIC values of 0.3 mg/ml against the P. gingivalis. Additionally, Fr2 displayed MBC value of 1 mg/ml against two types of bacteria. Fr4 showed an especially potent GTF inhibitory activity with IC50 value of 3.9 μg/ml. Fr1 displayed the best antioxidant activity with IC50 value of 1.8 μg/ml. The main compound in Fr1 was identified as gallic acid, and Fr2 was mostly a mixture of gallic acid and methyl gallate. Conclusions The results obtained in this study provide some scientific rationale and justify the use of this plant for the treatment of dental diseases in traditional medicine. A. nilotica bark, besides their antibacterial potentiality and GTF inhibitory activity, it may be used as adjuvant antioxidants in mouthwashes. Further studies in the future are required to identify the rest of the active compounds.

Wenzhouxiangella marina gen. nov, sp. nov, a marine bacterium from the culture broth of Picochlorum sp. 122, and proposal of Wenzhouxiangellaceae fam. nov. in the order Chromatiales

2015 ◽  
Vol 107 (6) ◽  
pp. 1625-1632 ◽  
Author(s):  
Guanghua Wang ◽  
Mingxing Tang ◽  
Tao Li ◽  
Shikun Dai ◽  
Huanlian Wu ◽  
...  
Keyword(s):  
Marine Bacterium ◽  
Culture Broth

scholarly journals SOLVENT FREE SYNTHESIS OF CHALCONE AS ANTIBACTERIAL SUBSTANCE

2017 ◽  
Vol 13 (1) ◽  
pp. 95
Author(s):  
Susy Yunita Prabawati ◽  
Arifah Khusnuryani ◽  
Khamidinal Khamidinal
Keyword(s):  
Antibacterial Activity ◽  
Solvent Free ◽  
Hydration Reaction ◽  
Antibacterial Substance ◽  
E Coli ◽  
H Nmr ◽  
Activity Test ◽  
Grinding Technique ◽  
Solvent Free Synthesis

<p>The aims of this study was to synthesize a compound of 3-methoxy-4-hydroxychalcone through Claisen-Schmidt condensation with grinding technique (solvent-free). Vanillin, acetophenone and 60 % NaOH catalyst used in this synthesis. Characterization of products was done with a spectrophotometer FTIR and <sup>1</sup>H-NMR spectrometer. The  product was obtained as a orange solid which has a melting point at    58 - 59 ˚C. Identification of the product by IR spectrophotometer showed the absorption of  C=C in the wavenumber of 1496.76 cm<sup>-1</sup> which confirmed that the hydration reaction of chalcone had occurred. Analysis using <sup>1</sup>H-NMR spectrometer also showed the proton of the CH=CH appearing on chemical shift (δ) 7.99 ppm. The antibacterial activity test showed that the compounds of 3-methoxy-4- hydroxychalcone has a potential as an antibacterial against bacteria <em>E. coli</em> and <em>B. subtilis</em>.</p>

scholarly journals Antibacterial Activity of Prismatomeris tetrandra K. Schum Root Extract against Antibiotic Resistance Bacteria

2018 ◽  
Vol 16 (5) ◽  
pp. 341-348
Author(s):  
On-Anong SOMSAP
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Culture Broth ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Root Extract ◽  
Gram Negative Bacteria ◽  
Antibiotic Resistant ◽  
Gram Positive Bacteria ◽  
Colony Forming Units

Antibiotic resistance bacteria has become an increasing problem now today due to many factors. This study investigates the efficacy of Prismatomeris tetrandra K. Schum root extract as a new source of antibacterial activity for antibiotic resistant bacteria using agar well diffusion method. The results showed that S. aureus TISTR517 exhibited more sensitivity to P. tetrandra K. Schum root extract than other Gram-positive bacteria indicator strains. On the other hand, Gram-negative bacteria exhibited resistance to P. tetrandra K. Schum root extract. The study further showed the activity between P. tetrandra K. Schum root extract and gentamycin (10 µg), it revealed that MRSA142 was resistant to gentamycin (10µg) but sensitive to P. tetrandra K. Schum root extract. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) was evaluated by using S. aureus TISTR517 and MRSA142 as indicator strains. The MIC value was 0.59 mg/mL and 1.17 mg/mL for S. aureus TISTR517 and MRSA142, respectively. MBC assay demonstrated that the MBC value was 9.75 mg/mL and 150 mg/mL for S. aureus TISTR517 and MRSA142 respectively. The mode of action was investigated with the presence of P. tetrandra K. Schum root extract in the culture broth. The action of P. tetrandra K. Schum root extract was revealed of bacteriostatic activity due to the Optical density (OD) at 600 nm and Colony-Forming Units (CFU) of indicator strains were continuously decreased.

scholarly journals Analisis Pigmen dan Aktivitas Antibakteri In Vitro Pigmen Astaksantin Kepiting (Grapsus albolineatus Lamarck) Jantan

2006 ◽  
Vol 8 (2) ◽  
pp. 160
Author(s):  
Aswan Thamin ◽  
Chairulwan Umar ◽  
Darussadah Paransa
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Enterobacter Cloacae ◽  
Bacteriostatic Activity ◽  
North Sulawesi ◽  
Marine Crustaceans ◽  
Activity Test ◽  
In Vitro Antibacterial Activity

Grapsus albolineatus is one of marine crustaceans which have carotenoid (astaxanthin) pigment. This research was conducted to analyze carotenoids (astaxanthin) extracted from G. albolineatus, and evaluate their in vitro antibacterial activity. The research was done in March-July 2002. Samples were collected from Manado Gulf, North Sulawesi. The result indicated that the carapace contained 4 carotenoids namely ß-caroten, ecinenon, astaxanthin diester, and astaxanthin monoester. In addition, the epidermis contained free astaxanthin. In vitro antibacterial activity test indicated that astaxanthin had low bacteriostatic activity against Psedomonas aeruginosa, Enterobacter cloacae, Staphylococcus aureus, and Proteus stuartii.

scholarly journals Antibacterial Activity Test of Bacteriocin from Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum Against Gram Positive Pathogenic Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 85
Author(s):  
Novi Permata Sari ◽  
Rafika Sari ◽  
Eka Kartika Untari
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Plantarum ◽  
Lactobacillus Casei ◽  
Pathogenic Bacteria ◽  
Heating Temperature ◽  
Lactobacillus Brevis ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Activity Test ◽  
Ph Range

Bacteriocin is a secondary metabolite product of lactic acid bacteria (LAB) which have an antimicrobial and potentially as a natural preservative. LAB isolates used in this study were Lactobacillus brevis, Lactobacillus casei and Lactobacillus plantarum. This study aimed to determine the antibacterial activity of bacteriocin produced by each isolate of LAB including the influence of pH and heating variation against Bacillus cereus, Bacillus subtilis and Staphylococcus epidermidis. Antibacterial activity test was done by using disc diffusion method. method. Confirmation test using proteolytic enzyme aimed to analyse that the inhibition zone produced from the activity of bacteriocin. The inhibition zone produced from L. brevis, L. casei and L. plantarum against B. cereus were 15.70, 16.43 and 14.50 mm, against B. subtilis were 13.37, 14.10 and 12.53 mm and against S. epidermidis were 11.37, 14.50 and 12.45 mm. The activity of each bacteriocin decreased with the addition of trypsin and catalase, bacteriocin was active in the pH range of 2-10 and heating temperature of 40-121oC. Statistical test showed that the addition of trypsin, catalase and the variation of pH also heating had significant differences (p<0.05) to antibacterial activity produced by bacteriocin from L. brevis, L. casei and L. plantarum. 

scholarly journals ANALYSIS OF SECONDARY METABOLITES, ANTIBACTERIAL ACTIVITY AND COMPOUND COMPOSITION IN THE SEA CUCUMBER Bohadschia sp. EXTRACT

2017 ◽  
Vol 8 (2) ◽  
pp. 645-653 ◽  
Author(s):  
Abdullah Rasyid
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Secondary Metabolites ◽  
Sea Cucumber ◽  
Similarity Index ◽  
Diffusion Method ◽  
Composition Analysis ◽  
Activity Test ◽  
Agar Diffusion Method ◽  
Color Reactions

Bohadschia sp. is one of the sea cucumber species that has potential to be developed as a source of antibacterial from the sea. Samples of sea cucumber Bohadschia sp. used in this study collected from the Ratai bay waters, Lampung. This study aims to determine the type of secondary metabolites, antibacterial activity and compound composition analysis containing in the sea cucumber extract. Identification of secondary metabolites by observation of color reactions, precipitation and foam. The method used to antibacterial activity test was the agar diffusion method, while identification of the composition of compounds performed with Gas Chromatogaphy-Mass Spectroscopy (GC-MS) method.Top of FormThe results showed that the type of secondary metabolites contained in the extract of sea cucumber Bohadschia sp. were steroids and saponins. The extract of sea cucumber Bohadschia sp. showed antibacterial activity against Bacillus subtilis and Vibrio eltor. Results of GC-MS were 12 compounds and have a similarity index same or more than 90%. All compounds consist of organosilicon cyclic, fatty acid, steroid, cyclo alkene and alkena. The compound with biggest abundance was cholest-5-en-3-yl nonanoate (4.89%) and retention time was 37.370 minutes.

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