scholarly journals ANALYSIS OF IFN- CONCENTRATION IN WISTAR RAT BLOOD AFTER ORAL ADMINISTRATION OF STANDARDIZED GREEN TEA WATER EXTRACT

2010 ◽  
Vol 10 (3) ◽  
pp. 390-395
Author(s):  
Djoko Agus Purwanto ◽  
Retno Pudji Rahayu ◽  
A. Toto Purnomo
Keyword(s):  
Oral Administration ◽  
Green Tea ◽  
Blood Concentration ◽  
Inhibitory Action ◽  
Epigallocatechin Gallate ◽  
Water Extract ◽  
Wistar Rat ◽  
Mechanisms Of Action ◽  
Chemopreventive Agents ◽  
Male And Female

Green tea and its polyphenols have been studied extensively as cancer chemopreventive agents in recent years. However, the mechanisms of action are still not clearly understood. Some researchers suggest that immune system plays important role to destroy cancer cells. Because of that reason, the present study was designed to analyse the effects of oral administration standardized green tea water extract on increasing of IFN-g blood concentration and to elucidate possible mechanisms involved in the inhibitory action of the cancer development. Two groups (male and female) of 5 rats have given p.o. administration 1.25% of standardized green tea water extract and got 300 mg of (-)-epigallocatechin gallate (EGCG)/kg body weight, while two groups others (male and female) were used as control. We found that IFN-g blood concentration on male and female Wistar rat are significantly increase with 13.11% and 17.59%, respectively (p

scholarly journals THE IMPACT OF GREEN TEA ON BLOOD FLUIDITY IMPROVEMENT AND WEIGHT LOSS

Jurnal NERS ◽  
2017 ◽  
Vol 9 (1) ◽  
pp. 1
Author(s):  
Djoko Agus Purwanto ◽  
Asri Darmawati ◽  
Purwaningsih Purwaningsih
Keyword(s):  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Water Extract ◽  
Wistar Rat ◽  
High Fructose ◽  
Treatment Of Diabetes ◽  
The Impact ◽  
Passage Times

Introduction: Various studies have shown the benefi cial effects of green tea, not only on cardiovascular diseases butalso on type 2 diabetes. Method: In this study, the preparation of green tea water extract has been standardized to (-)-epigalocatechin gallate (EGCG), the major component of green tea. The role of green tea water extract on blood fl uidityand diabetes diseases has been studied in 13 Fructose-Fed Rat (FFR). The rats were given high fructose diet ad libitumfor one week and then combination with green tea water extract every day for 6 days. Results: The results show, greentea water extract can reduces 100 μL blood passage times of wistar rat signifi cantly (p<0.01) by Micro-Channel ArrayFlow Analyzer (MC-FAN) instrument. Green tea water extract also had strong effect in reducing abdominal fat (p<0.05),blood glucose level (p<0.01) and body weight (p<0.01). Discussion: These results suggest that green tea water extractmay has benefi cial effects for the treatment of diabetes and reduce blood viscosity.Keywords: green tea, (-)-epigallocatechin gallate, HPLC, blood fl uidity, fructose-fed rat

scholarly journals Potential Therapeutic Targets of Epigallocatechin Gallate (EGCG), the Most Abundant Catechin in Green Tea, and Its Role in the Therapy of Various Types of Cancer

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3146 ◽  
Author(s):  
Saleh A. Almatroodi ◽  
Ahmad Almatroudi ◽  
Amjad Ali Khan ◽  
Fahad A. Alhumaydhi ◽  
Mohammed A. Alsahli ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Mechanisms Of Action ◽  
Chemopreventive Agents ◽  
Cancer Management ◽  
Anti Cancer ◽  
Chemopreventive Effect ◽  
Cell Signaling Pathways

Epigallocatechin-3-gallate (EGCG), an active compound of green tea and its role in diseases cure and prevention has been proven. Its role in diseases management can be attributed to its antioxidant and anti-inflammatory properties. The anti-cancer role of this green tea compound has been confirmed in various types of cancer and is still being under explored. EGCG has been proven to possess a chemopreventive effect through inhibition of carcinogenesis process such as initiation, promotion, and progression. In addition, this catechin has proven its role in cancer management through modulating various cell signaling pathways such as regulating proliferation, apoptosis, angiogenesis and killing of various types of cancer cells. The additive or synergistic effect of epigallocatechin with chemopreventive agents has been verified as it reduces the toxicities and enhances the anti-cancerous effects. Despite its effectiveness and safety, the implications of EGCG in cancer prevention is certainly still discussed due to a poor bioavailability. Several studies have shown the ability to overcome poor bioavailability through nanotechnology-based strategies such as encapsulation, liposome, micelles, nanoparticles and various other formulation. In this review, we encapsulate therapeutic implication of EGCG in cancer management and the mechanisms of action are discussed with an emphasis on human clinical trials.

scholarly journals Aldose Reductase Differential Inhibitors in Green Tea

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1003 ◽  
Author(s):  
Francesco Balestri ◽  
Giulio Poli ◽  
Carlotta Pineschi ◽  
Roberta Moschini ◽  
Mario Cappiello ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Green Tea ◽  
Aldose Reductase ◽  
Active Site ◽  
Inhibitory Action ◽  
Computational Study ◽  
Epigallocatechin Gallate ◽  
Polyol Pathway ◽  
Differential Inhibition ◽  
Tea Extract

Aldose reductase (AKR1B1), the first enzyme in the polyol pathway, is likely involved in the onset of diabetic complications. Differential inhibition of AKR1B1 has been proposed to counteract the damaging effects linked to the activity of the enzyme while preserving its detoxifying ability. Here, we show that epigallocatechin gallate (EGCG), one of the most representative catechins present in green tea, acts as a differential inhibitor of human recombinant AKR1B1. A kinetic analysis of EGCG, and of its components, gallic acid (GA) and epigallocatechin (EGC) as inhibitors of the reduction of L-idose, 4-hydroxy2,3-nonenal (HNE), and 3-glutathionyl l-4-dihydroxynonanal (GSHNE) revealed for the compounds a different model of inhibition toward the different substrates. While EGCG preferentially inhibited L-idose and GSHNE reduction with respect to HNE, gallic acid, which was still active in inhibiting the reduction of the sugar, was less active in inhibiting HNE and GSHNE reduction. EGC was found to be less efficient as an inhibitor of AKR1B1 and devoid of any differential inhibitory action. A computational study defined different interactive modes for the three substrates on the AKR1B1 active site and suggested a rationale for the observed differential inhibition. A chromatographic fractionation of an alcoholic green tea extract revealed that, besides EGCG and GA, other components may exhibit the differential inhibition of AKR1B1.

scholarly journals Implications of Green Tea and Its Constituents in the Prevention of Cancer via the Modulation of Cell Signalling Pathway

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Arshad H. Rahmani ◽  
Fahad M. Al shabrmi ◽  
Khaled S. Allemailem ◽  
Salah M. Aly ◽  
Masood A. Khan
Keyword(s):  
Green Tea ◽  
Biological Activities ◽  
Cell Signalling ◽  
Epigallocatechin Gallate ◽  
Chemopreventive Agents ◽  
Beneficial Effects ◽  
Prevention And Treatment ◽  
Prevention Of Cancer ◽  
Cell Signalling Pathway ◽  
Cell Signaling Pathways

Green tea is commonly used as a beverage worldwide, especially in China, Japan, Morocco, and Saudi Arabia. Green tea and its constituents have been considered very effective in the prevention and treatment of various diseases. It contains a variety of catechins, which show a pivotal role in the modulation of biological activities and also act as chemopreventive agents. Earlier studies have confirmed that green tea and its chief constituent epigallocatechin gallate (EGCG) have a potential role in the management of cancer through the modulation of cell signaling pathways. In this review, we focused on the beneficial effects of green tea and its constituents in the cancer prevention and treatment and its impact on modulation of molecular pathways.

Studies on the effects of oral administration of nutrient mixture, quercetin and red onions on the bioavailability of epigallocatechin gallate from green tea extract

2009 ◽  
Vol 24 (S1) ◽  
pp. S48-S55 ◽  
Author(s):  
Anup Kale ◽  
Sonia Gawande ◽  
Swati Kotwal ◽  
Shrirang Netke ◽  
Waheed Roomi ◽  
...  
Keyword(s):  
Oral Administration ◽  
Green Tea ◽  
Epigallocatechin Gallate ◽  
Green Tea Extract ◽  
Tea Extract

scholarly journals Evaluation of Acute and Chronic Toxicities of the Water Extract from Ziziphus attopensis Pierre

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Seewaboon Sireeratawong ◽  
Supaporn Vannasiri ◽  
Urarat Nanna ◽  
Tipaya Singhalak ◽  
Kanjana Jaijoy
Keyword(s):  
Body Weight ◽  
Oral Administration ◽  
Health Monitoring ◽  
Chronic Toxicity ◽  
Water Extract ◽  
The Body ◽  
Oral Feeding ◽  
Female Rats ◽  
Male And Female ◽  
Organ Weights

We studied an acute and chronic oral toxicity of the extract from Ziziphus attopensis (ZA) in male and female SD rats according to the OECD guidelines. After a single oral administration of ZA 5 g/kg body weight, measurement of the body and organs, necropsy, and health monitoring were performed. The body and organ weights and behavior were not changed relative to the control rats indicating that ZA does not produce acute toxicity. The chronic toxicity was determined by oral feeding both male and female rats daily with ZA at the doses of 1, 2, 4, and 8 g/kg body weight for 180 days. Body weight changes, hematological and biochemical parameters, organ weights, gross finding, and histopathology examination were monitored during the experimental period. The results did not show any differences from the control groups. Analyses of these results with the information of signs, behavior, and health monitoring can lead to a conclusion that the long-term oral administration of ZA for 180 days does not cause chronic toxicity.

Isolation of Epigallocatechin Gallate from Green Tea and its Effects on Probiotics and Pathogenic Bacteria

2017 ◽  
Vol 17 (1) ◽  
pp. 69-77
Author(s):  
Tu Lijun ◽  
Sun Hanju ◽  
He Shudong ◽  
Zhu Yongsheng ◽  
Yu Ming ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Green Tea ◽  
Lactobacillus Acidophilus ◽  
Pathogenic Bacteria ◽  
Epigallocatechin Gallate ◽  
Microbial Populations ◽  
Medium Ph ◽  
Bioactive Substance ◽  
Tea Extract

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

Effect of Green Tea and (-)-Epigallocatechin Gallate on the Pharmacokinetics of Rosuvastatin

2020 ◽  
Vol 21 (6) ◽  
pp. 471-478
Author(s):  
Shenjia Huang ◽  
Qingqing Xu ◽  
Linsheng Liu ◽  
Yicong Bian ◽  
Shichao Zhang ◽  
...  
Keyword(s):  
Green Tea ◽  
Cell Model ◽  
Hek293t Cell ◽  
Epigallocatechin Gallate ◽  
Drug Uptake ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Time Curve ◽  
Uptake And Transport

Background: Green tea can inhibit OATPs, so it may interact with the substrate of OATPs, such as rosuvastatin. Objective: This study aimed to investigate the effects of green tea on the pharmacokinetics of rosuvastatin and its mechanism. Methods: Male Sprague-Dawley rats received different doses of green tea extract (GTE) and (-)- epigallocatechin-3- gallate (EGCG). Caco-2 cells and OATP1B1-HEK293T cells were used in drug uptake and transport assay. The matrix concentrations of rosuvastatin and catechins were determined by ultra-performance liquid chromatographytandem mass spectrometry (UPLC-MS/MS). Results: GTE and EGCG were both found to increase the area under the plasma concentration-time curve (AUC0-∞) of rosuvastatin ((p<0.050). In the Caco-2 cell model, the uptake and transport of rosuvastatin in the GTE groups were 1.94-fold (p<0.001) and 2.11-fold (p<0.050) higher, respectively, than those of the control group. However, in the EGCG group, the uptake and transport of rosuvastatin were decreased by 22.62% and 44.19%, respectively (p<0.050). In the OATP1B1- HEK293T cell model, the OATP1B1-mediated rosuvastatin uptake was decreased by GTE to 35.02% of that in the control (p<0.050) and was decreased by EGCG to 45.61% of that in the control (p<0.050). Conclusion: GTE increased the systemic rosuvastatin exposure in rats. The mechanism may include an increase in rosuvastatin absorption and a decrease in liver distribution by inhibiting OATP1B1. EGCG may be the main ingredient of green tea that affects the pharmacokinetic parameters of rosuvastatin. Our results showed the importance of conducting green tea-rosuvastatin study.

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