scholarly journals IN VITRO BIOACTIVITY TEST OF IRRADIATED MAHKOTA DEWA BARK [Phaleria macrocarpa (Scheff.) Boerl.] AGAINST HUMAN CANCER CELL LINES

2012 ◽  
Vol 12 (1) ◽  
pp. 43-48
Author(s):  
Ermin Katrin Winarno
Keyword(s):  
Ethyl Acetate ◽  
Gamma Irradiation ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Irradiation Dose ◽  
Ethyl Acetate Extract ◽  
Human Cancer ◽  
Cytotoxic Potential ◽  
Ic50 Values ◽  
Phaleria Macrocarpa

Gamma irradiation has been used to preserve an herbal medicine, but it has not been known the effects of gamma irradiation on their bioactivity as an anticancer agent yet. In the previous study, the gamma irradiation on mahkota dewa bark with the optimum dose of 7.5 kGy could be used for decontamination of bacteria and fungus/yeast. In this report, the effect of gamma irradiation with the dose of 7.5 kGy on the bioactivities of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl.) bark against leukemia L1210 cells was studied. The control and irradiated samples were successively macerated with n-hexane and ethyl acetate. In the previous results, silica gel column chromatography of ethyl acetate extract of non irradiated sample (control) gave 8 fractions. Among these fractions, fraction 6 indicated the most cytotoxic-potential fraction, so that in this experiment, the ethyl acetate extract of irradiated and non irradiated sample were fractionated with the same manner as previous fractionation. The fraction 6 obtained both from control and irradiated samples were then assayed their inhibitory activities against 4 kinds of human cancer lines, i.e. HeLa, THP-1, HUT-78 and A-549. The results showed that the fraction 6 from control sample gave IC50 values of 3.65, 5.59, 3.55, and 4.06 µg/mL, against HeLa, THP-1, HUT-78 and A-549, respectively, meanwhile fraction 6 from irradiated sample gave IC50 values of 8.26, 7.02, 5.03, and 5.59 µg/mL, respectively. Gamma irradiation dose of 7.5 kGy on mahkota dewa bark could decreased the cytotoxic activity of fraction 6 as the most cytotoxic-potential fraction against HeLa, THP-1, HUT-78 and A-549 cancer cell lines, but decreasing the cytotoxic activity has not exceeded the limit of an extract and the fraction declared inactive. So that the irradiation dose of 7.5 kGy can be use for decontamination of bacteria and fungus/yeast without eliminating the cytotoxic activity.

scholarly journals Bioactivity-guided isolation of alkamides from a cytotoxic fraction of the ethyl acetate extract of Anacyclus pyrethrum (L.) DC. roots.

2018 ◽  
Vol 31 (4) ◽  
pp. 180-185
Author(s):  
Souad Hamimed ◽  
Nadji Boulebda ◽  
Hocine Laouer ◽  
Abdelmalik Belkhiri
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethyl Acetate Extract ◽  
Human Cancer ◽  
Colorectal Cancer Cell Line ◽  
Cytotoxic Activities ◽  
Root Extract ◽  
Alcohol Extract

Abstract Introduction. The alcohol extract of Pellitory (Anacyclus pyrethrum) roots has been previously shown to exert anticancer activities on the Human Colorectal Cancer Cell Line (HCT) by targeting apoptosis, metastasis and cell cycle arrest. However, the nature of the cytotoxic molecules associated with this activity remains unexplored. Aims. This study aims to reinvestigate Pellitory root extract as regard to its cytotoxic activity and to proceed to a bioguided fractionation to explore its active fraction and to give new insight in their phytochemical constituents. Methods. Powdered roots were subjected to repeated extraction with Petroleum ether (Pe), Chloroform (Ch), Ethyl acetate (Ea) and Methanol (Me). Pellitory extracts were then screened for cytotoxic activity using the Brine Shrimp Lethality (BSL) bioassay. Results. Ea extract exhibited a marked cytotoxic activity, with LC50 of 249.26 μg/mL in the BSL bioassay. The remaining extracts (Pe,Ch,Me) treated groups exhibited no or low mortality in the range of tested concentrations (1-1000 µg/mL). BSL assay-guided chromatographic fractionation of Ea active Extract revealed a highly cytotoxic fraction (F11) with LC50 of 42.5 µg/mL. Multistep purifications of the active F11 fraction afforded four alkamides, namely N-isobutyldeca-2,4-dienamide or Pellitorine (I), N-propyldodeca- -2,8-dienamide (II), N-isobutyltetradeca-2,4-dienamide (III) and N-propylnona-2,5- -dienamide (IV). Conclusions. This study suggests that cytotoxic activity is localized mainly in the ethyl acetate extract (Ea) of pellitory roots. BSL assay fractionation of this active extract leads to the isolation of four alkamides, including pellitorine (I). While this isobutyl alkamide has previously shown strong cytotoxic activities against human cancer cell lines, the other compounds (II to IV) were not previously reported as cytotoxic. Subsequently, the isolated alkamides will be considered in future study as candidates for in depth in-vitro evaluation of their cytotoxicity against cancer and normal cell lines. Finally, through this study, BSL assay demonstrate again its usefulness as bench-top assay in exploring plant extracts for cytotoxic compounds.

Cytotoxic Activity of Flavonoids and Extracts from Retama sphaerocarpa Boissier

2000 ◽  
Vol 55 (1-2) ◽  
pp. 40-43 ◽  
Author(s):  
Miguel López-Lázaro ◽  
Carmen Martín-Cordero ◽  
Felipe Cortés ◽  
Joaquín Piñero ◽  
María Jesús Ayuso
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Human Cancer ◽  
Human Cancer Cell ◽  
Srb Assay ◽  
Retama Sphaerocarpa ◽  
Human Cancer Cell Lines ◽  
Aerial Parts ◽  
Dependent Inhibition

Abstract Seven flavonoids isolated from chloroform , ethyl acetate and butanol extracts, obtained from the aerial parts of Retama sphaerocarpa, have been assessed for cytotoxic activity against three human cancer cell lines: TK-10 (renal adenocarcinom a), MCF-7 (breast adenocarcinom a) and UACC-62 (m elanom a), using the SRB assay. All of them , extracts and flavonoids, were actives in, at least, one of the three cell lines at the recom m ended N ational C ancer Institute doses. They produce a d ose-dependent inhibition of cell grow th at concentrations in the 10-6-10-4 ᴍ and 25 -250 μg/ml range for the flavonoids and extracts respectively, being the flavonol rhamnazin the most cytotoxic.

scholarly journals Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines

2019 ◽  
Vol 10 (1) ◽  
pp. 37
Author(s):  
Poppy Anjelisa Zaitun Hasibuan ◽  
Panal Sitorus ◽  
Denny Satria ◽  
Rizka Damela Sibuea
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Mcf7 Cell ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
T47d Cell ◽  
Flavonoid Content ◽  
Dpph Method ◽  
Plectranthus Amboinicus

Research into plants with anticancer effects is actively encouraged in orderto discover new drugs with lessertoxicity but more potent effects. The aims of study are to evaluate the antioxidant properties and to investigate the cytotoxic activity of Plectranthus amboinicus (Lour.) Spreng. leaves ethyl acetate fractions on HeLa,T47D and MCF7 cell lines. The extract was prepared by graded maceration using n-hexane and ethyl acetate. The ethyl acetate extract was fractionated in vacuum liquid chromatography with n-hexane: ethyl acetate; and ethyl acetate: methanol as mobile phase. Then, the fractions were analyzed with thin layer chromatography (TLC). The free radical scavenging activity was measured by DPPH method, the total flavonoid content was calculated by quercetin equivalent and the absorbance is measured by using UV-Visible spectrophotometry. The cytotoxic activity were determined using MTT assay. The fractions contained 5 sub fractions with same TLC profile. The fractions showed antioxidant activity by DPPH method with different IC50 values, namely: 130 µg/mL(I), 127 µg/mL(II), 137 µg/mL(III), 129 µg/mL(IV), and 124 µg/ mL(V), respectively. The measurement of total flavonoid content showed 118 mg QE/g (I), 50 mg QE/g (II), 207 mg QE/g (III), 56 mg QE/g (IV), and 55 mg QE/g (V). The IC50 of each sub fractions on HeLa cell were 77 µg/mL, 46 µg/mL, 93 µg/mL, 71 µg/mL and 476 µg/mL; for T47D cell were 1621 µg/mL, 111 µg/mL, 128 µg/mL, 150 µg/mL and 209 µg/mL; and for MCF7 were 259 µg/mL, 343 µg/mL, 575 µg/mL, 408 µg/mL and 250 µg/mL. Based on the results, the fractions derived from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. leaves exhibit antioxidant. The Fraction II from ethyl acetate extract of Plectranthus amboinicus (Lour.) Spreng. was the most cytotoxic on HeLa, T47D and MCF7 cell lines. It is potential to undergo further isolation of its cytotoxic compounds.Keywords : antioxidant, cytotoxic, Plectranthus amboinicul (Lour.) Spreng., ethyl acetate fractions

The Evaluation of Potential Cytotoxic Effect of Different Proton Pump Inhibitors on Different Human Cancer Cell Lines

2020 ◽  
Vol 20 (2) ◽  
pp. 245-253
Author(s):  
Aya Qasem ◽  
Violet Kasabri ◽  
Eman AbuRish ◽  
Yasser Bustanji ◽  
Yusuf Al-Hiari ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Raw 264.7 ◽  
Human Cancer Cell ◽  
Raw 264.7 Macrophages ◽  
Human Cancer Cell Lines ◽  
Ic50 Values

Objective : To assess the differential cytotoxic activity of PPIs on different human cancer cell lines; namely A549 lung cancer, CACO-2 colorectal cancer, MCF-7 breast cancer, and PANC-1 pancreatic cancer, A375 skin melanoma. Methods: In this study, the five human cancer cell lines and human non-cancerous fibroblasts were treated with increasing concentration of PPIs Omeprazole (OMP), Esomeprazole (ESOM), and Lansoprazole (LANSO) (50-300μM), over 24h, 48h, and 72h. Cell viability was determined using 3-(4,5- Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and the IC50 values of PPIs were measured. The most sensitive cell line A375 was used for further investigation. The cytotoxic effects of LANSO on these cells were assessed using Annexin-V Propidium Iodide (AV-PI) flow cytometry. As of action mechanism; anti-inflammatory effects of each PPIs and PPIs-DOXO combination therapy on LPS-stimulated RAW 264.7 mouse macrophages were assessed. Results: Dose and time dependence cytotoxic activity of PPIs on human cancer cell lines was founded. Unlike DOXO; All PPIs had a selective cytotoxic effect in the normal fibroblasts. Unlike the equipotent OMP and ESOM; LANSO was the most potent drug with IC50 values at 72h of 99, 217, 272, 208, 181μM against A375, A549, CACO-2, MCF-7, and PANC-1, respectively. AV-PI flow cytometry revealed dose-dependent apoptotic effects of LANSO alone and substantially enhanced in DOXO-co-treatments. Interestingly unlike ESOM and OMP, LANSO proved more effective than indomethacin in LPS-stimulated RAW 264.7 macrophages. None of the tested compounds, as well as indomethacin, exerted any cytotoxicity against RAW 264.7 macrophages. PPIs-DOXO lacked potential synergistic combination antiinflammation therapies. Conclusion: This study provides the evidence that PPIs induce a direct and differential cytotoxic activity against human cancer cell line by the induction of the apoptosis. Moreover, PPIs increase cancer cell lines sensitivity to doxorubicin via apoptosis augmentation. Nevertheless, PPIs-DOXO lacked potential synergistic combination therapies in either antiproliferation or anti-inflammation.

Antiradical and Cytotoxic Activity of Different Helichrysum Plicatum Flower Extracts

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Dubravka Bigović ◽  
Katarina Šavikin ◽  
Teodora Janković ◽  
Nebojša Menković ◽  
Gordana Zdunić ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Total Phenolic Content ◽  
Antiradical Activity ◽  
Phenolic Content ◽  
Human Cancer ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Moderate Activity

Flowers of Helichrysum plicatum were extracted under different experimental conditions, and their antioxidant activity was determined by DPPH radical scavenging assay. Extracts obtained with higher concentration of ethyl acetate (90% or 100%) were found to contain the greatest amount of total phenolics (> 250 mg gallic acid equivalents/g of dried extract), and high correlation between total phenolic content and antiradical activity was observed (r = −0.79). Based on the total phenolic content and antiradical activity, some extracts were selected for investigation of cytotoxic activity toward PC3, HeLa and K562 human cancer cell lines in vitro. All tested extracts exhibited moderate activity against HeLa cells (41.9-42.1 μg/mL), whereas the extract obtained with 100% ethyl acetate was the most active against K562 and PC3 cell lines (25.9 and 39.2 μg/mL, respectively). Statistical analysis revealed significant correlation between total phenolic content and cytotoxic activity against PC3 and K562 cells. HPLC identification of phenolic compounds from the extracts indicated the presence of apigenin, naringenin and kaempferol as free aglycones, and glycosides of apigenin, naringenin, quercetin and kaempferol. Among aglycones, kaempferol displayed moderate cytostatic activity against all cell lines (24.8-64.7 μM).

scholarly journals Acanthomine A, a new Pyrimidine-β-Carboline Alkaloid from the Sponge Acanthostrongylophora Ingens

2008 ◽  
Vol 3 (2) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Sabrin R. M. Ibrahim ◽  
RuAngelie Ebel ◽  
Rainer Ebel ◽  
Peter Proksch
Keyword(s):  
Mass Spectrometry ◽  
Nmr Spectroscopy ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Brine Shrimp ◽  
Ethyl Acetate Extract ◽  
Cancer Cell Lines ◽  
Chemical Investigation

Chemical investigation of the ethyl acetate extract of the sponge Acanthostrongylophora ingens afford one new pyrimidine-β-carboline alkaloid named acanthomine A (2), together with two known compounds annomontine (1) and 1,2,3,4-tetrahydronorharman-1-one (3). Their structures were unambiguously established on the basis of NMR spectroscopy (1H, 13C, 1H-1H COSY, HMQC and HMBC) and mass spectrometry. The isolated compounds were tested for cytotoxic activity using brine shrimp bioassay and different cancer cell lines.

Identification of Flavonoids and Bufadienolides and Cytotoxic Effects of Kalanchoe daigremontiana Extracts on Human Cancer Cell Lines

Planta Medica ◽  
2020 ◽  
Vol 86 (04) ◽  
pp. 239-246 ◽  
Author(s):  
Justyna Stefanowicz-Hajduk ◽  
Monika Asztemborska ◽  
Mirosława Krauze-Baranowska ◽  
Sylwia Godlewska ◽  
Magdalena Gucwa ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Human Cancer ◽  
Ethanol Extract ◽  
Traditional Use ◽  
Human Cancer Cell Lines ◽  
Water Fraction ◽  
Ic50 Values ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Abstract Kalanchoe species are well-known medicinal plants used in traditional medicine as anti-inflammatory and analgesic remedies. Recently, it has been reported that Kalanchoe plants have cytotoxic properties; however, data on traditional use of these plants in tumor treatment are extremely limited. Kalanchoe daigremontiana is one of the most popular species cultivated in Europe, and it is used, among other things, as a remedy in treating skin injuries and wounds. Studies on the biological activity of this species are scarce, and there is a lack of data on the cytotoxic activity of K. daigremontiana extracts on epithelial cancer cells in the literature. In our present study, we analyzed the phytochemical composition of K. daigremontiana ethanol extract and fractions–water and dichloromethane–by the HPLC-DAD-ESI-MS method and estimated cytotoxic activity of the extracts on human adenocarcinoma (HeLa), ovarian (SKOV-3), breast (MCF-7), and melanoma (A375) cell lines by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, real-time cell analyzer (RTCA), and flow cytometry. We identified 6 bufadienolide compounds and 19 flavonoids, mostly kaempferol, quercetin, isorhamnetin, and myricetin glycosides, of which only 3 flavonoids have been identified in K. daigremontiana to date. Other flavonoids that were characterized in our study have not yet been found in this plant. The ethanol extract and water fraction of K. daigremontiana did not show significant cytotoxic activity on the tested cell lines. In contrast, the dichloromethane fraction showed the strongest activity against all cell lines with IC50 values of ≤ 10 µg/mL. The results indicated that this activity is mainly due to the presence of bersaldegenin-1,3,5-orthoacetate.

scholarly journals Studying on cytotoxic activity of ethyl acetate extracts and isolated substances from cultured Isaria cicadae F0004 in Vietnam against the MCF-7 cell lines and Jurkat cell lines

2021 ◽  
Vol 947 (1) ◽  
pp. 012037
Author(s):  
Nguyen Chi Dung ◽  
Ha Thi Ngoc ◽  
Pham Thi My Ninh ◽  
Dang Hoang Phu ◽  
Dinh Minh Hiep ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Jurkat Cell ◽  
Protocatechuic Acid ◽  
Fruit Body ◽  
Preparative Chromatography ◽  
Ic50 Values ◽  
Mcf 7

Abstract Isaria cicadae species of the Isaria genus have been isolated in many Asian countries, including China, Korea, Thailand, and Vietnam. Furthermore, Isaria cicadae content potential medicinal source in the prevention and treatment of cancer, strengthening the immune system. This study aims to investigate the cytotoxicity against MCF-7 and Jurkat T cell lines of the extract and isolated potential compounds from Isaria cicadae in Vietnam. The results showed that ethyl acetate (EA) extract from the fruit body of the Isaria cicadae F0004 strain had high cytotoxic activity against both MCF-7 and Jurkat cell lines at the concentration of 100 μg/mL with the IC50 value was reached 17.15 ± 1.68 and 10.37 ± 0.61 μg/mL respectively. The constituents of the EA extract from the fungus Isaria cicadae F0004 were isolated by column chromatography and preparative chromatography. Then, the structures were determined by spectroscopy 1H-NMR, 13C-NMR. It was obtained 5 compounds including uracil, 1-0-ethyl-ß-D-ribofuranose, ergosterol, p-hydroxybenzoic acid, protocatechuic acid. Protocatechuic acid isolated from Isaria cicadae F0004 that showed cytotoxic activity on MCF-7 and Jurkat cell lines with IC50 values of 5.97 ± 0.36 and 3.15 ± 0.64 (mM) respectively. This study is the basis for further research on the cytotoxic activity of the fungus Isaria cicadae F0004 in vitro.

scholarly journals Synthesis and In Vitro Cytotoxic Activity of Chromenopyridones

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Balwinder Singh ◽  
Vishal Sharma ◽  
Gagandeep Singh ◽  
Rakesh Kumar ◽  
Saroj Arora ◽  
...  
Keyword(s):  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Human Cancer ◽  
Cancer Cell Lines ◽  
Hep G2 ◽  
Cytotoxic Potential ◽  
Human Cancer Cell Lines ◽  
Mcf 7

Novel substituted chromenopyridones (3a–j and 6a–d) were synthesized and evaluated in vitro for the cytotoxic activity against various human cancer cell lines such as prostate (PC-3), breast (MCF-7), CNS (IMR-32), cervix (Hela), and liver (Hep-G2). preliminary cytotoxic screening showed that all the compounds possess a good to moderate inhibitory activity against various cancer cell lines. Particularly, compound 6b bearing allyl moiety displayed a significant cytotoxic potential in comparison to standard drugs.

scholarly journals Khasiat dan Profil Kromatogram Fraksi Aktif dari Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) yang Diiradiasi

2017 ◽  
Vol 15 (2) ◽  
pp. 160
Author(s):  
Ermin Katrin ◽  
Setiananda Jacobs ◽  
Hendig Winarno
Keyword(s):  
Ethyl Acetate ◽  
Gamma Irradiation ◽  
Irradiation Dose ◽  
Ethyl Acetate Extract ◽  
Leukemia Cells ◽  
Heating Process ◽  
Cytotoxicity Test ◽  
L1210 Leukemia ◽  
Fruit Peel ◽  
Garcinia Mangostana

The mangosteen fruit peel (Garcinia mangostana L.) are used as anti-infl ammatory, antihistamine, treatment of heart disease, antibacterial, antifungal, it is also used for the treatment or therapy of cancer, because it has a cytotoxic activity against cancer cells. Most of Indonesian people have used the mangosteen fruit peel and they produce mangosteen peel into powder or herbal medicine industry has produced its extract in capsules. Efforts to preserve dried mangosteen rind has done by heating process or by gamma irradiation technique. This study aimed to study the effect of gamma irradiation on cytotoxicity activity of extracts and active fractions mangosteen fruit peel against L1210 leukemia cells and chromatogram profi le of extracts and active fractions of the mangosteen fruit peel. Dry powder mangosteen rind irradiated using gamma irradiation dose of 5; 7.5; 10; and 15 kGy. Then each sample was successive macerated in n-hexane, ethyl acetate, and ethanol. Each extract was tested cytotoxicity against L1210 leukemia cells. The ethyl acetate extract was most active extract (IC50 = 4.17 mg/mL) compared with n-hexane extract (IC50 = 8.29 mg/mL) and ethanol extract (IC50 = 7.52 mg/mL). Fractionation of ethyl acetate extract by column chromatography were obtained 6 fractions. The result of cytotoxicity test showed that fraction 1 was the most active fraction (IC50 = 3.97 mg/mL), it was it was still categorized as potential anticancer (IC50 ≤ 20 mg/mL). Profi le chromatogram of fraction 1 with TLC-densitometry showed patches of discoloration on irradiation dose of 10 and 15 kGy. The results of analysis by HPLC fraction 1 showed a decrease of peak area at a dose of 10 kGy was signifi cantly different from the control. Based on the chromatogram profi le of fraction 1 and itscytotoxicity against L1210 leukemia cells, so the maximum dose of 7.5 kGy gamma irradiation can  beap plied on irradiation of mangosteen fruit peel without changing its effi cacy as anti-cancer agent.

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