scholarly journals Two Isophalerin Compounds from Ethyl Acetate of Leave and Fruit of Mahkota Dewa (Phaleria macrocarpa (Scheff.) Boerl.) and Its Antibacterial Activity

2015 ◽  
Vol 15 (2) ◽  
pp. 179-186
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Spectroscopic Method ◽  
Amorphous Solid ◽  
Traditional Medicines ◽  
E Coli ◽  
Weak Activity ◽  
Phaleria Macrocarpa ◽  
Isolated Compound

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is belong to family of Thymelaeaceae is one of Indonesia's traditional medicines. The aim of this research is to isolate secondary metabolites from ethyl acetate extract of leave and fruit of mahkota dewa and to determine the molecular structure of isolated compounds using spectroscopic method and to know the antibacterial activity of the isolated compound. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The compounds were separated and purified with column chromatography. The compound 1 was isolated from ethyl acetate extract of leave as white needle amorphous solid as 45 mg. The compound was identified by spectroscopic as 4,6-dihydroxy-4’-methoxybenzophenon-2-O-β-D-glucopyranoside and named isophalerin B. From the test results of antibacterial activity showed that the compound 1 (10 mg/mL) in ethanol has a weak activity against the bacteria S. aureus and E. coli. The compound 2 was isolated from ethyl acetate extract of fruit as peach needle crystal as 10 mg. The compound was identified by spectroscopic as 4,6-dihydroxy-4’-methoxybenzophenon-2-O-α-D-glucopyranoside and named isophalerin A.

scholarly journals Antibacterial activity of endosymbiotic fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands, Jakarta

2021 ◽  
Vol 948 (1) ◽  
pp. 012069
Author(s):  
R Trifani ◽  
Noverita ◽  
T A Hadi ◽  
E Sinaga
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Marine Sponges ◽  
Disc Diffusion ◽  
Active Compounds ◽  
E Coli ◽  
Stylissa Carteri ◽  
Further Development

Abstract Endosymbiont fungi from marine sponges are a rich source of medicinally active compounds. Indonesia has a huge number of marine sponges. This research was conducted to determine the antibacterial potential of endosymbiont fungi isolated from marine sponges collected from Kotok Kecil Island, Seribu Islands. The screening was conducted with two methods, the modified GIBEX and disc diffusion. Species of marine sponges were isolated, namely Petrosia sp., Stylissa carteri, Cinachyrella australiensis, Callyspongia sp., Petrosia nigrians, and Stylissa massa, and obtained 9 isolates of endosymbiont fungi. The GIBEX test against Escherichia coli and Streptococcus mutans showed that ethyl acetate extract had strongest antibacterial activity. Disc diffusion test of ethyl acetate extract, five isolates had antibacterial activity against S. mutans and four against E. coli. The isolate that showed the strongest antibacterial activity was from Petrosia sp. Which has an inhibition zone of 8.4 mm against E. coli and 7.45 mm against S. mutans. The main active compounds from the isolate of Petrosia sp. are butylhydroxytoluene and phthalic acid di-(2-propylpentyl) esters. Based on this study, we concluded that the endosymbiont fungi of marine sponges are potential to be developed for further development as source of antibacterial agents.

scholarly journals ANTIOXIDANT ACTIVITY OF 2,6,4’-TRIHYDROXY-4-METHOXY BENZOPHENONE FROM ETHYL ACETATE EXTRACT OF LEAVES OF MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.)

2011 ◽  
Vol 11 (2) ◽  
pp. 180-185 ◽  
Author(s):  
Susilawati Susilawati ◽  
Sabirin Matsjeh ◽  
Harno Dwi Pranowo ◽  
Chairil Anwar
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
1H Nmr ◽  
Column Chromatography ◽  
13C Nmr ◽  
Ethyl Acetate Extract ◽  
Chemical Constituent ◽  
Traditional Medicines ◽  
Phaleria Macrocarpa ◽  
Spherical Crystal

Mahkota dewa plant (Phaleria macrocarpa (Scheff.) Boerl.) which is included into family of Thymelaeaceae is one of Indonesia's traditional medicines. Chemical constituent has been isolated from ethyl acetate extract of leaves of mahkota dewa. Sample was extracted with methanol, concentrated then extracted by n-hexane, chloroform and ethyl acetate. The ethyl acetate extract was separated and fractionated by column chromatography. The first fraction was purified by TLC preparative and recrystalization. Compound was isolated as red-brown spherical crystal in 8 mg (m.p. 129-131 °C). Its spot gave dark fluoroscence at TLC plate (UV366) with Rf of 0.3 at TLC chromatogram with eluent of n-hexane : ethyl acetate (7:3); 0.6 with n-hexane : ethyl acetate (1:1); 0.9 with -hexane : ethyl acetate (4:6). This compound was dissolved in methanol. Compound was identified by UV, IR, 1H NMR, 13C NMR and NMR 2 dimension (HMQC, COSY, HMBC and DEPT-135) spectroscopic as 2,6,4'-trihydroxy-4-methoxybenzophenon. This compound as well as the ethyl acetate extract showed antioxidant activity on DPPH with IC50 was 10.57 and 101.06 μg/mL, respectively. This compound showed strong antioxidant activity on DPPH, almost to the standard antioxidant activity of quercetin (IC50 of 2.93 μg/mL)

scholarly journals Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria

2021 ◽  
Vol 9 (2) ◽  
pp. 75
Author(s):  
Luthfiah Luthfiah ◽  
Dwi Setyati ◽  
Sattya Arimurti
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Antibacterial Properties ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Well Diffusion Method

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.

scholarly journals Uji Aktivitas Sitotoksik dan Antibakteri Ekstrak Daun Tumbuhan Rengas (Gluta renghas L)

2020 ◽  
Vol 11 (1) ◽  
pp. 52-60
Author(s):  
Sanusi Ibrahim ◽  
Suryati Suryati ◽  
Enda Desriansyah Aziz
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Inhibition Zone ◽  
Cytotoxicity Activity ◽  
E Coli ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of ◽  
Ethyl Acetate Extracts

Generally, Gluta renghas L. is known because of its very toxic latex which can cause hard irritations to skin. Although, rengas latex has efficacy as an antibacterial agent. Related to previous research, it has been reported that there are urisol, rengol, glutarengol, laccol, and thitsiol in rengas latex. The woody trunk was reported to contain flavonoid, benzenoid, lipid, and steroid compounds. The leaves, were reported shows that the leaves of G. renghas contain an anticholinesterase substance and can be reduced the rate of hydrolysis of acetylcholine. Extraction of rengas leaves has been carried out. It has been shown phenols, steroids, and coumarins compounds in methanol extract, flavonoids, phenols, saponins and alkaloids compounds in ethyl acetate extract, steroids and alkaloids compounds in hexane extract. In the present, cytotoxicity and antibacterial activity have been tested. The results show that the great cytotoxicity activity by ethyl acetate extracts which have an LC50 value is 123,718 µg/mL (R2 0.9822), while the great antibacterial activity shown by methanol extract 1,000 µg/mL with a diameter of inhibition zone 19.02 mm (S. aureus) and 16.06 mm (E. coli). 

scholarly journals Isolation and Identification of Secondary Metabolite from Marine Sponge Callyspongia sp. and its Antibacterial Potency

2020 ◽  
Vol 11 (3) ◽  
pp. 10082-10088
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Marine Sponge ◽  
Ethyl Acetate Extract ◽  
Aldehyde Group ◽  
Isolation And Identification ◽  
E Coli ◽  
Microdilution Method ◽  
Antibacterial Susceptibility ◽  
Vacuum Liquid Chromatography

Marine sponge Callyspongia sp. is one full of potency as a source for discovering and developing novel antibacterial. This study aims to isolate the Callypsongia sp. and assay their antibacterial activity. Callyspongia sp. were macerated with ethyl acetate (3x24 hrs), isolated with vacuum liquid chromatography (VLC) and RC (radial chromatography), and determined their structure with 1H and 13C-NMR. The antibacterial activity was assayed with the microdilution method. From ethyl acetate extract of Callyspongia sp. was successfully 2 isolated compounds, namely, isolate C1 (cholesterol) and isolate C2 (Unknown alkaloid with carbonyl from aldehyde group). The extract has MIC>512 µg/mL against Bacillus subtilis, Escherichia coli, Streptococcus mutans, and Salmonella enterica. While in both isolates provided MIC value >256 µg/mL against B. subtilis, E. coli, and S. mutans, yet in S. enterica provided 128 µg/mL for isolate C1 and 256 µg/mL for isolate C2. In conclusion, ethyl acetate extract of Callyspongia sp. contains cholesterol and Unknown alkaloid with carbonyl from the aldehyde group, and they both exhibited low antibacterial susceptibility.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

scholarly journals Isolasi dan Identifikasi Metabolit Sekunder dari Ekstrak Etil Asetat Batang Nangka (Artocarpus heterophyllus Lmk.) dan Aktivitasnya Sebagai Antibakteri

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 81-89
Author(s):  
Indriani ◽  
Iswan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flavonoid Compound ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Isolation And Identification ◽  
Artocarpus Heterophyllus ◽  
Isolated Compound ◽  
Concentration Series

Isolation and identification of secondary metabolite of ethyl acetate extract of jackfruit stem (Artocarpus heterophyllus Lmk.) as well as its activity as antibacterial have been carried out. The purpose of this study was to know the characterization and antibacterial activity of secondary metabolites from ethyl acetate extract of the Jackfruit stem. Ethyl acetate extract was fractionated and purified using several chromatographic techniques. The identification of the isolated compound was elucidated by spectroscopic UV-Vis, IR, and NMR. Based on data analysis of UV, IR, and NMR spectra showed that the isolated compound was a flavonoid derivative. Antibacterial activity test of the isolated compound to Escherichia coli and Staphylococcus aureus was done by a well diffusion method. The inhibitory zone values of flavonoid compound in concentration series of 1000 µg/ml, 3000 µg/ml, and 5000 µg/ml against E. coli were 9.65 mm, 10.25 mm, and 11.00 mm, respectively. While, the inhibitory zone values in the same concentration series against  S. aureus were 9.00 mm, 10.75 mm, and 11.38 mm.  Keywords: Jackfruit, Artocarpus heterophyllus Lmk., flavonoid, antibacterial activity.

scholarly journals ANTIBACTERIAL ACTIVITY OF XANTHONE FROM ETHYL ACETATE EXTRACT OF THE STEAM BARK OF Garcinia picrorrhiza Miq

2017 ◽  
Vol 13 (2) ◽  
pp. 250 ◽  
Author(s):  
Muharni Muharni ◽  
Elfita Elfita ◽  
Emil Pertiwi
Keyword(s):  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Salmonella Typhi ◽  
Separation And Purification ◽  
Chromatography Method ◽  
Staphyloccocus Aureus ◽  
Isolated Compound

A compound was isolated from the ethyl acetate extract of stem bark <em>Garcinia picrorrhiza</em>. The extraction was conducted by maseration. Separation and purification were done by chromatography method. The structure of compound was established using UV, IR, and <sup>1</sup>H NMR spectroscopy. The antibacteria activity of the isolated compound was tested by paper disk difusion method and minimum inhibitory concentration (MIC)   value was determined by using well difusion method examined against bacteria <em>Escherechia coli, Salmonella typhi, Bacillus subtilis</em> and <em>Staphyloccocus aureus</em>. The isolated compound was a yellow solids (43.8 mg) with melting point 171 – 172 <sup>o</sup>C. Based on spectroscopy data compared with data from the literature, the isolated  compound is a known compound of oxygenated xanthone group with structure 1,4,5-trihydroxy-2-(3methylbut-2-enyl)xanthone. The compound exhibited an antibacterial activity against <em>Bacillus subtilis</em> only with MIC of 62.5 µg/mL.

3-Acetoxy-6-benzoyloxyapangamide from Achyranthes aspera

1970 ◽  
Vol 4 (2) ◽  
Author(s):  
Md. Abdul Aziz ◽  
Md. Mizanur Rahman ◽  
Anjon Kumar Mondal ◽  
Tanvir Muslim ◽  
Azizur Rahman ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Bacillus Cereus ◽  
Key Words ◽  
Full Text ◽  
Ethyl Acetate Extract ◽  
Spectroscopic Techniques ◽  
Achyranthes Aspera ◽  
Isolated Compound

3-Acetoxy-6-benzoyloxyapangamide (1) has been isolated from an ethyl acetate extract of the stem of Achyranthes aspera. The structure of the isolated compound was established by modern spectroscopic techniques. The extract was found to show mild antibacterial activity against Bacillus cereus. Key words: Achyranthes aspera, 3-acetoxy-6-benzoyloxyapangamide, Antibacterial activity. Dhaka Univ. J. Pharm. Sci. Vol.4(2) 2005 The full text is of this article is available at the Dhaka Univ. J. Pharm. Sci. website

scholarly journals Study of Phytochemicals, Toxicity, Antibacterial Activity of Ethyl Acetate Leaf Extract Extract (Paperomiapellucida L)

2020 ◽  
Vol 3 (2) ◽  
pp. 74
Author(s):  
Ida Duma Riris ◽  
Tita Juwitaningsih ◽  
Destria Roza ◽  
Marini Damanik ◽  
Albinus Silalahi
Keyword(s):  
Antibacterial Activity ◽  
Rural Communities ◽  
Herbal Medicine ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Inhibitory Zone ◽  
E Coli ◽  
Activity Test

Suruhan Leaf (Paperomiapellucida L) is a weedy plant that is used by rural communities as herbal medicine. In this study, phytochemical tests have identified compounds contained in the ethyl acetate extract, then the toxicity test was carried out by the Brint Shrimp LethallytyTocycity (BSLT) method using Artemia Salina Leach shrimp larvae, an antibacterial activity test for bacteria: Bacillus cereus (ATCC) 11778), S. aureus (ATCC 25923), E. coli (ATCC 25922), S. saprophytic (ATCC 49907), Catsobacterfrendi (ATCC 18090), P.acne (ATCC 27853), Epidermis (ATCC 12228) with diffusion methods, various of concentration 1; 5; 10; 25% as control used chloramphenicol 30 mcg, the highest inhibitory zone at a concentration of 25% showed a strong antibacterial succession of 20.7; 11.1; 29.7; 15; 13.5; 10; 17.3 mm. The results of the identification of compounds in ethyl acetate extract are alkaloids, flavonoids, steroids, saponins, and tannins. The toxicity of LC50 is 71.28 ppm (toxic).

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