scholarly journals A Novel Spectrophotometric Method for Determination of Chloramphenicol Based On Diazotization Reaction at Room Temperature

2018 ◽  
Vol 16 (1) ◽  
pp. 32 ◽  
Author(s):  
Abdul Wafi ◽  
Ganden Supriyanto ◽  
Tjitjik Srie Tjahjandarie
Keyword(s):  
Spectrophotometric Method ◽  
Coupling Agent ◽  
Analytical Method ◽  
Room Temperature ◽  
Zinc Powder ◽  
Reducing Agent ◽  
Bismuth Nitrate ◽  
Time Response ◽  
Nitrate Pentahydrate

An analytical method for determination of chloramphenicol (CAP) based on the diazotization reaction at room temperature has been developed. The CAP was reduced using zinc powder (Zn) and diazotization reaction was carried out at room temperature in the presence of NaNO2, bismuth nitrate pentahydrate (Bi(NO3)3.5H2O) as catalyst. 2-napthol was used as coupling agent to form a red-violet solution and the absorbance was measured by UV-Vis spectrophotometer at 554 nm. The optimization of analytical parameters including reducing agent, catalyst, coupling agent and time response were 0.15 g, 0.15 g, 230.67 µg/mL and 8-9 min respectively.

scholarly journals Oxcarbazepine: validation and application of an analytical method

2010 ◽  
Vol 46 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Paula Cristina Rezende Enéas ◽  
Renata Barbosa de Oliveira ◽  
Gerson Antônio Pianetti
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Disintegration Time ◽  
International Conference ◽  
Pharmacopoeial Monograph ◽  
Development And Validation ◽  
Excipient Compatibility

Oxcarbazepine (OXC) is an important anticonvulsant and mood stabilizing drug. A pharmacopoeial monograph for OXC is not yet available and therefore the development and validation of a new analytical method for quantification of this drug is essential. In the present study, a UV spectrophotometric method for the determination of OXC was developed. The various parameters, such as linearity, precision, accuracy and specificity, were studied according to International Conference on Harmonization Guidelines. Batches of 150 mg OXC capsules were prepared and analyzed using the validated UV method. The formulations were also evaluated for parameters including drug-excipient compatibility, flowability, uniformity of weight, disintegration time, assay, uniformity of content and the amount of drug dissolved during the first hour.

scholarly journals Analytical Method Validation of Azadirachtin Extracted From Azadirachta Indica

2017 ◽  
Vol 2 (04) ◽  
pp. 99-106
Author(s):  
Syed Imran Ul Haq ◽  
Tribhuvan Singh ◽  
Shaik Mohammed Salman ◽  
Afifa Namreen ◽  
Anas Rasheed
Keyword(s):  
Regression Analysis ◽  
Statistical Analysis ◽  
Azadirachta Indica ◽  
Spectrophotometric Method ◽  
Drug Concentration ◽  
Analytical Method ◽  
Distilled Water ◽  
Analytical Method Validation ◽  
Accuracy And Precision

A simple UV spectrophotometric method for the determination of Azadirachtin is developed exhibiting maximum absorbance using distilled water at 542nm.The present study is based on the detection of sample drug concentration using dichloro methane as diluent. All the conditions required were optimized. Statistical analysis was carried out and results of which were satisfactory. The optical characteristics such as absorption maxima, regression analysis and correlation coefficient are obtained. Recovery studies were close to 100% that indicate the accuracy and precision of proposed method and the non-interference of the formulation excipients. All the valid parameters are summarized.

Analytical Method Development and Validation of Prucalopride Succinate in Bulk and Formulation by UV-Visible Spectrophotometry

2021 ◽  
pp. 4189-4191
Author(s):  
A.C. Bhosale ◽  
V.C. Bhagat ◽  
V. V Kunjir ◽  
D.P. Kardile ◽  
R.V. Shete
Keyword(s):  
Quantitative Determination ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Method Development ◽  
Routine Analysis ◽  
Ich Guidelines ◽  
Method Development And Validation ◽  
Uv Visible ◽  
Development And Validation

Purpose: Analytical method development and validation for the quantitative determination of Prucalopride succinate in bulk and tablet formulation which plays major role in the development and manufacture of pharmaceuticals. Methods: In the present work a simple, rapid and reproducible UV-Visible Spectrophotometric method was developed and validated according to ICH guidelines. Results and Conclusions: The parameters linearity, specificity, precision, accuracy, and robustness were studied. The wavelength 243nm was selected for the estimation of drug using methanol as a solvent. The drug obeys Beer-lambert’s law over the concentration range 2-10μg/ml. The accuracy of the method was assessed by recovery studies and was found between 97.2- 98.3 %. The method was successfully applied for routine analysis of Prucalopride succinate in bulk and formulation.

scholarly journals Validation of a UV-spectrophotometric analytical method for determination of LPSF/AC04 from inclusion complex and liposomes

2015 ◽  
Vol 51 (1) ◽  
pp. 183-191
Author(s):  
Rafaela Siqueira Ferraz ◽  
Elisângela Afonso Moura Mendonça ◽  
Jéssica Priscila Avelino Silva ◽  
Isabella Macário Ferro Cavalcanti ◽  
Mariane Cajubá Britto Lira-Nogueira ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Low Cost ◽  
Health Surveillance ◽  
Validation Parameters ◽  
Significant Difference ◽  
Detection And Quantification ◽  
Sensitive Spectrophotometric Method

The aim of this study was to develop and validate a UV spectrophotometric method for determination of LPSF/AC04 from inclusion complex and encapsulated into liposomes. The validation parameters were determined according to the International Conference on Harmonisation (ICH) and National Health Surveillance Agency (ANVISA) guidelines. LPSF/AC04 was determined at 250 nm in methanol by a UV spectrophotometric method, exhibiting linearity in the range from 0.3 to 2 µg.mL−1 (Absorbance=0.18068 x [LPSF/AC04 µg.mL-1] + 0.00348), (r2=0.9995). The limits of detection and quantification were 0.047µg.mL−1 and 0.143µg.mL−1, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had coefficient of variation of less than 5% and no statistically significant difference between theoretical and practical concentrations was detected. Thus, a rapid, simple, low cost and sensitive spectrophotometric method was developed and validated for determining the content of inclusion complex and liposomes containing LPSF/AC04.

scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

scholarly journals STUDI OPTIMASI PENENTUAN UNSUR HARA BESI DALAM CAMPURAN TANAH DAN KOMPOS MENGGUNAKAN METODE SPEKTROFOTOMETRI

2015 ◽  
Vol 8 (2) ◽  
pp. 143
Author(s):  
Refilda ◽  
Suhartini ◽  
Indrawati
Keyword(s):  
Citric Acid ◽  
Metal Ions ◽  
Spectrophotometric Method ◽  
Incubation Time ◽  
Iron Content ◽  
Nutrient Content ◽  
Reducing Agent ◽  
Regression Equation ◽  
Complex Stability

Determination of iron nutrient content in a mixture of soil and compost with spectrophotometric method has been carried out. Metal ions Fe(II) complexed with 0.1% ortho-fenantrolin 2 mL, 0.01 M citric acid as reducing  agent  using acetat buffer at pH 4.5 and its complex was measured by using spectrophotometer UV/Vis at wavelength 515 nm, the complex stability for was 60 minutes . The highest nutrient of iron with content in soil:compost with ratio of 0.8:0.2 was 0.92% at incubation time 30 days. The lowest iron content in the mixture of soil:compost (1:0) at incubation time for  15 days was 0.58% that obtained by the regression equation y = 95.85x + 0.025 with R2=0.992. The level of nutrient iron in compost obtained lower than the maximum regulated by SNI 2.00%.

scholarly journals A kinetic spectrophotometric method for the determination of lansoprazole in pharmaceutical formulations

2006 ◽  
Vol 71 (10) ◽  
pp. 1107-1120 ◽  
Author(s):  
Nafisur Rahman ◽  
Zehra Bano ◽  
Hejaz Azmi ◽  
Mohammad Kashif
Keyword(s):  
Spectrophotometric Method ◽  
Room Temperature ◽  
Pharmaceutical Formulations ◽  
Accuracy And Precision ◽  
Significant Difference ◽  
Kinetic Spectrophotometric ◽  
Comparison Of The Results ◽  
Calibration Equations ◽  
Method Show

Asimple kinetic spectrophotometric method has been developed for the determination of lansoprazole in pharmaceutical formulations. The method is based on the oxidation of the drug with alkaline potassium permanganate at room temperature. The reaction was followed spectrophotometrically by measuring the increase in the absorbance owing to the formation of MnO 42? at 610 nm (Method A) and the decrease in the absorbance at 530 nm due to the disapperance of MnO4? (Method B). Calibration procedures were adopted for the assay of the drug. The calibration curves were linear over the concentration ranges of 5-150 and 5-70?g ml-1, with the corresponding calibration Equations: rate = -3.915x10-6 + 5.271x10-5 c and ?A = 1.04x 10-3 + 1.78x10-3 c for methods A, and B, respectively. A statistical comparison of the results of the proposed procedures with those of the reference spectrophotometric method show excellent agreement and indicated no significant difference between the compared methods in terms of accuracy and precision. Interval hypothesis tests were also performed, which indicated that the true bias of all samples was less than ? 2 %. .

Spectrophotometric Determination of Benomyl and Methyl 2-Benzimidazole Carbamate in Product Formulations by a Low Temperature-Solubilization Technique

1979 ◽  
Vol 62 (3) ◽  
pp. 488-493
Author(s):  
Mikio Chiba
Keyword(s):  
Low Temperature ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Room Temperature ◽  
Solvent Mixture ◽  
Cold Temperature ◽  
Simple Equation ◽  
Wettable Powder ◽  
Diluted Solution

Abstract A rapid spectrophotometric method has been developed to determine benomyl (methyl l-(butylcarbamoyl)- 2-benzimidazole carbamate) and methyl 2-benzimidazole carbamate (MBC) simultaneously in wettable powder formulations by solubilizing all the ingredients in a special solvent mixture. A special solvent mixture consisting of chloroform, dioxane, and water was prepared at room temperature; the mixture was cooled to 1°C and used to dissolve samples. The degradation of benomyl, unavoidable at 25°C, was eliminated by this cold temperature operation. Absorbance of the diluted solution was measured at 294 and 286 nm in a ratiorecording spectrophotometer, and individual concentrations of benomyl and MBC were calculated by a simple equation. An alternative method has been suggested for users of nonautomatic scanning spectrophotometers. re-Butyl isocyanate was used to stabilize benomyl in standard solutions and to re-form benomyl from MBC. Analyses were conducted on Benlate® 50% WP and Benlate® 10% WP mixed with captan 50%. Most 50% WP formulations contained 50-55% intact benomyl and 0.4-2.3% MBC. Benomyl was stable in those WP formulations, although both time and conditions of storage varied significantly. Most 10% WP samples contained 8–12% benomyl and 0.1–2.9% MBC.

scholarly journals A Rapid and Direct Sensitive Spectrophotometric Method for the Determination of Molybdenum(VI) using Gallacetophenone Phenylhydrazone with Application to Alloy Steel Samples

2011 ◽  
Vol 8 (3) ◽  
pp. 1362-1370 ◽  
Author(s):  
D. Venkataramana Reddy ◽  
P. V. Chalapathi ◽  
A. Varada Reddy
Keyword(s):  
Alloy Steel ◽  
Spectrophotometric Method ◽  
Room Temperature ◽  
Molar Absorptivity ◽  
Analysis Method ◽  
Slope Analysis ◽  
Relative Standard ◽  
Analytical Reagent ◽  
Ph Range

A rapid, sensitive and fairly selective spectrophotometric method has been developed for the determination of molybdenum(VI) using gallacetophenone phenylhydrazone (GPPH) as an analytical reagent. The GPPH forms an orange red species of molybdenum(VI) at a pH range of 3.0-4.0 and the complex is soluble in 20% ethanol. The Mo(VI)-GPPH complex shows maximum absorbance at 375 nm with molar absorptivity and Sandellʼs sensitivity being 1.539x104L/mol cm and 1.5x10-3μg cm-2respectively. The system obeys Beerʼs law in the range of 1.0-6.0 μg/mL. The repeatability of the method was checked by finding the relative standard deviation. The composition of the Mo(VI)-GPPH complex is established as 1:2 by slope analysis method. The instability constant of the complex calculated from Edmond and Birnbaumʼs method is 6.785x10-8at room temperature. The interfering effect of various cations and anions has also been studied. The method has been applied for the determination of Mo(VI) in alloy steel samples.

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