scholarly journals Simultaneous Spectrophotometric Method for Determination of Emtricitabine and Tenofovir Disoproxil Fumarate in Three-Component Tablet Formulation Containing Rilpivirine Hydrochloride

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
S. Venkatesan ◽  
N. Kannappan
Keyword(s):  
Spectrophotometric Method ◽  
Analytical Method ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Tablet Dosage

Developing a single analytical method for estimation of individual drug from a multidrug composition is a very challenging task. A complexation, derivatization, extraction, evaporation, and sensitive-free direct UV spectrophotometric method is developed and validated for the simultaneous estimation of some antiviral drugs such as emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), and rilpivirine HCl (RPV) in tablet dosage form by Vierordt’s method. The solutions of standard and sample were prepared in methanol. The λmax⁡ for emtricitabine, tenofovir disoproxil fumarate, and rilpivirine hydrochloride were 240.8 nm, 257.6 nm, and 305.6 nm, respectively. Calibration curves are linear in the concentration ranges 4–12 μg/ml for EMT, 6–18 μg/ml for TDF, and 0.5–1.5 μg/ml for RPV, respectively. Results of analysis of simultaneous equation method were analyzed and validated for various parameters according to ICH guidelines.

scholarly journals Simultaneous UV Spectrophotometric Estimation of Amlodipine and Chlorthalidone in Bulk and Combined Tablet Dosage Form

2019 ◽  
pp. 468-472
Author(s):  
Sunil More ◽  
Ashpak Tamboli ◽  
Snehal Patil ◽  
Amol Vhanmane
Keyword(s):  
Spectrophotometric Method ◽  
Mobile Phase ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Equation ◽  
Ich Guidelines ◽  
Combined Estimation ◽  
Tablet Dosage ◽  
Different Levels

There is not a single analytical methods appeared in the literature for combined estimation of Amlodipine and Chlorthalidone in tablets dosage form. Attempts were made to develop a simple, precise and accurate Simultaneous UV spectroscopic method of Amlodipine and Chlorthalidone in bulk and Amlodac CH tablet dosage form by using simultaneous equation method. UV spectrophotometric method was developed and validated as per ICH guidelines using methanol as mobile phase. Amlodipine and Chlorthalidone individually follows the Beer-Lamberts law over concentration range 2.5-12.5μg/ml and 6.25-31.5μg/ml, Regression of coefficient was found to be r2=0.999 and r2=0.999 respectively. The percentage recovery was found in the range of 98% to 102% at three different levels. The proposed method was successfully applied for the determination of Amlodipine and Chlorthalidone in tablets dosage form as per ICH guidelines the result of the analysis were validated statistically and were found to be satisfactory.

scholarly journals New Simple Spectrophotometric Method for the Simultaneous Estimation of Paracetamol and Flupirtine Maleate in Pure and Pharmaceutical Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
P. Giriraj ◽  
T. Sivakkumar
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Precision Study ◽  
Tablet Dosage ◽  
Concentration Levels

A new, simple, precise, accurate, reproducible, and efficient Vierordt’s method or simultaneous equation method was developed and validated for simultaneous estimation of paracetamol and flupirtine maleate in pure and pharmaceutical dosage form. The method was based on the measurement of absorbance at two wavelengths 245 nm and 344.5 nm, λmax of paracetamol and flupiritine maleate in 0.1 N HCl correspondingly. Calibration curves of paracetamol and flupiritine maleate were found to be linear in the concentration ranges of 5–15 μg/mL and 1.53–4.61 μg/mL, respectively, with their correlation coefficient values (R2) 0.999. LOD and LOQ were 185.90 ng/mL and 563.38 ng/mL for paracetamol and 78.89 ng/mL and 239.06 ng/mL for flupiritine maleate. In the precision study, the % RSD value was found within limits (RSD<2%). The percentage recovery at various concentration levels varied from 99.18 to 100.02% for paracetamol and 98.47 to 100.09% for flupiritine maleate confirming that the projected method is accurate. It could be concluded from the results obtained in the present investigation that this method for simultaneous estimation of paracetamol and flupirtine maleate in pure and tablet dosage form is simple, accurate, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of paracetamol and flupiritine maleate in pure and pharmaceutical dosage form.

scholarly journals Simultaneous Estimation and Validation of Artemether and Lumefantrine by UV Spectrophotometry in Tablet

2021 ◽  
Vol 11 (2) ◽  
pp. 16-22
Author(s):  
Megha Mishra ◽  
Anand Mundada
Keyword(s):  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Dosage Form ◽  
Spectroscopic Method ◽  
Simultaneous Estimation ◽  
Uv Spectrophotometry ◽  
Ich Guidelines ◽  
Curve Method ◽  
Tablet Dosage

A UV spectrophotometric method has been developed for the simultaneous determination of Artemether and Lumefantrine. The spectroscopic method for estimation of Artemether and Lumefantrine employed Area under curve method for analysis using Ethanol as solvent. Artemether has absorbance maxima 253.2 nm and Lumefantrine has absorbance maxima 235.2 nm and both these drugs obey Beer's law in concentration range of 4.24 -67.84 μg/ml for Artemether and 4.68 -28.08 μg/ml for Lumefantrine. The recovery studies ascertained the accuracy of the purposed method and the results were validated as per ICH guidelines. The results were found satisfactory and reproducible. The method was applied successfully for the estimation of Artemether and Lumefantrine in tablet dosage form without the interference of common excipients. Keywords: Artemether, Lumefantrine; Area under curve; Simultaneous; Estimation

scholarly journals UV Spectrophotometric Determination of Hydrochlorothiazide and Olmesartan Medoxomil in Pharmaceutical Formulation

2010 ◽  
Vol 7 (4) ◽  
pp. 1156-1161 ◽  
Author(s):  
A. T. Hemke ◽  
M. V. Bhure ◽  
K. S. Chouhan ◽  
K. R. Gupta ◽  
S. G. Wadodkar
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Olmesartan Medoxomil ◽  
Simultaneous Equation ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Ich Guidelines ◽  
Routine Work ◽  
Reproducible Method

A simple, specific, accurate, precise and reproducible method has been developed and validated for the simultaneous estimation of hydrochlorothiazide and Olmesartan medoxomil in combined dosage form by UV spectrophotometric method. UV spectrophotometric method includes Simultaneous equation method (Method I) 271.5 nm and 257.0 nm λmaxof both the drugs were selected, absorbance Ratio method (Method II) 261.5 nm an isoabsorptive wavelength and 257.0 nm were selected for estimation of hydrochlorothiazide and Olmesartan medoxomil respectively and Three-wavelength method (Method III), two wavelengths were selected such that hydrochlorothiazide give same absorbances (263.8 and 278.4 nm) at two selected wavelength while third wavelength (316.5 nm) was such that olmesartan gives nearly zero absorbance. The two drugs follow Beer’s law over the concentration range of 5-25 μg/mL. The % recoveries of the both the drugs were found to be nearly 100 % representing the accuracy of the proposed methods. Validation of the proposed methods was carried out for its accuracy, precision, specificity and ruggedness according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of hydrochlorothiazide and olmesartan medoximil in combined dosage form.

DERIVITISATION OF PREGABALIN FOR SIMULTANEOUS ESTIMATION WITH METHYLCOBALAMINE USING ULTRAVIOLET SPECTROSCOPY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (11) ◽  
pp. 70-72
Author(s):  
Sampada D. Dalvi ◽  
◽  
Pramod L. Ingale ◽  
Sohan Chitlange
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Drug Combinations ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Ultraviolet Spectroscopy ◽  
Beer's Law ◽  
Beer’S Law ◽  
Tablet Dosage

Accurate, sensitive, and economical procedures for simultaneous estimation of pregabalin and methylcobalamine in tablet dosage form have been developed. The method employed is simultaneous equation method. This method employs the formation and solving of simultaneous equation using 528 nm and 568 nm as the two wavelengths for forming equation. Drugs obey Beer’s law in the concentration range 10-50 µg mL-1 for pregabalin and 10-50 µg mL-1 for methylcobalamine. The proposed spectrophotometric method was validated and successfully applied for the assay of both drug combinations in several laboratory prepared mixtures and commercial tablets

DEVELOPMENT AND VALIDATION OF SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF FAMOTIDINE, DICLOFENAC AND PARACETAMOL IN THEIR COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (09) ◽  
pp. 60-64
Author(s):  
P. P Desai ◽  
◽  
N. R. Patel ◽  
H. G Bhatt
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Simultaneous Estimation ◽  
Absorbance Measurement ◽  
Validation Parameters ◽  
Stock Solutions ◽  
Development And Validation ◽  
Tablet Dosage

Famotidine (FAM), Diclofenac (DCF) and Paracetamol (PCM) are used in combination for musculoskeletal disorders. A simple, sensitive, rapid, precise, reproducible and accurate spectrophotometric method for simultaneous determination of FAM, DCF and PCM was developed. The method was based on UV spectrophotometric determination of three drugs using simultaneous equation method. The stock solutions were prepared in methanol AR. Absorbance measurement was carried out at 288.4 nm, 281.2nm and 248.2 nm for FAM, DCF and PCM respectively. Beer Lambert law was obeyed in the concentration range of 1-30μg/mL for FAM, 2-40μg/mL for DCF and 1-20μg/mL for PCM. The results of the analysis were tested and validated for various validation parameters statistically and by recovery studies according to the International Conference on Harmonization Q2B guidelines. The utility of the developed method has been demonstrated by analysis of commercially available tablet dosage form.

scholarly journals SIMULTANEOUS UV SPECTROPHOTOMETRIC METHODS FOR ESTIMATION OF CEFADROXIL AND PROBENECID IN TABLET DOSAGE FORM

2018 ◽  
Vol 1 (3) ◽  
pp. 19-23
Author(s):  
Mayur S. Jain ◽  
Sunil R. Bavaskar ◽  
Shashikant D. Barhate ◽  
Jintendra D. Fegade
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Ratio Method ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Spectrophotometric Methods ◽  
Absorption Ratio ◽  
Tablet Dosage

Two methods for simultaneous estimation of Cefadroxil and Probenecid in combined tablet dosage form have been developed. The first UV spectrophotometric method was a determination using the simultaneous equation method at 233 nm and 247 nm. The second UV spectrophotometric method is the Q – analysis (absorption ratio) method, which involves the formation of absorbance equation at 242 nm (Isobestic point) and at 247 nm the maximum absorption of Probenecid . The linearity ranges for Cefadroxil and Probenecid both were 10-60μg/ml respectively. The accuracy of the methods was assessed by recovery studies was found to be 99.43±0.75 and 99.69±0.40 for simultaneous equation method and 99.23±0.34 and 99.56±0.16 for absorption ratio method for Cefadroxil and Probenecid respectively. These methods are simple, accurate and rapid; those require no preliminary separation and can therefore be used for routine analysis of both drugs in quality control laboratories.

scholarly journals Quantification of Benazepril Hydrochloride and Hydrochlorothiazide in Tablet Dosage Form by Simultaneous Equation Spectrophotometric Method

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Varsha Parmar ◽  
Usmangani Chhalotiya ◽  
Dimal Shah ◽  
Kashyap Bhatt ◽  
Sunil Baldania
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Tablet Dosage

Simple, accurate, precise, reproducible, requiring no prior separation, and economical procedures for simultaneous estimation of benazepril hydrochloride (BEN) and Hydrochlorothiazide (HCT) in tablet dosage form have been developed. Simultaneous equation method employs for estimation of both drugs in methanol at 240 nm and 270 nm as two analytical wavelengths. BEN and HCT at their respective λmax (240 nm and 270 nm) show linearity in a concentration range of 2–12 μg/mL and 4–14 μg/mL. Recovery studies for BEN are 100.0–100.6% and 99.8–100.0% for HCT in the case of simultaneous equation method confirming the accuracy of the proposed method. The proposed method is recommended for routine analysis since it is rapid, simple, accurate and also sensitive and specific.

scholarly journals Development and Validation of Spectrophotometric Methods for Simultaneous Estimation of Furosemide and Spironolactone by Vierordt’s Method in Bulk and Combined Tablet Dosage Form

2018 ◽  
Vol 26 (1) ◽  
pp. 74-90 ◽  
Author(s):  
Rohankumar R. Chavan ◽  
Somnath D. Bhinge ◽  
Mangesh A. Bhutkar ◽  
Dheeraj S. Randive
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Spectrophotometric Methods ◽  
Bulk Drug

Abstract Anew simple, convenient and suitable spectrophotometric method for simultaneous determination of Furosemide and Spironolactone in combined dosage form has been developed and validated. Simultaneous equation method (Vierordt’s method) was used for determination of Furosemide and Spironolactone in combined dosage form. For spectrophotometric method development double distilled water and ethanol were used as a solvent in the ratio of (20:80). The proposed method was quantitatively evaluated in terms of linearity, precision, accuracy, lower limit of detection (LOD) and quantification (LOQ), recovery and robustness. All the parameters were found to be within the acceptance limit. λmax of Furosemide and Spironolactone was found to be 275 and 237 nm respectively. Beer’s law was obeyed over the concentration ranges of 2-10 μg mL−1 for both Furosemide and Spironolactone respectively. The % assay for commercial formulation was found to be 99.60%±0.0500 for Furosemide and 100.26%±1.17 for Spironolactone by the proposed methods. The overall recovery was observed to be 100.38±0.09% for Furosemide and 100.49±0.4197% for Spironolactone by simultaneous equation method (Vierordt’s method). LOD and LOQ were 0.76 and 2.32 μg mL−1 for Furosemide, 1.99 and 6.04 μg mL−1 for Spironolactone. A new simple, convenient, precise, rapid, accurate and economical and reliable spectrophotometric method was developed and validated for the analysis of Furosemide and Spironolactone in bulk drug and their formulations.

DEVELOPMENT OF STABILATING INDICATING ANALYTICAL METHOD FOR FAMOTIDINE AND DICLOFENAC POTASSIUM IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2012 ◽  
Vol 49 (06) ◽  
pp. 40-42
Author(s):  
D. R. Chaple ◽  
◽  
S. A Mehta ◽  
M. P Yeole ◽  
P. S. Tarte
Keyword(s):  
Analytical Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Routine Analysis ◽  
Simultaneous Estimation ◽  
Tablet Dosage

This work deals with the simultaneous estimation of famotidine and diclofenac potassium in a tablet dosage form. The method employed is simultaneous equation. The absorption maxima at 266 nm and 286 nm were used for the estimation of famotidine and diclofenac, respectively. Both the drugs and their mixture obey Beer-Lamberts law at selected wavelength in given concentration range. The result of analysis has been validated statistically and recovery studies confirmed the accuracy of the proposed method. The proposed method is simple, rapid, precise and accurate. It is used for the routine analysis of both drugs.

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