scholarly journals SIFAT FISIKOKIMIAWI SELULOSA PRODUKSI ISOLAT BAKTERI Gluconacetobacter xylinus KRE-65 PADA METODE FERMENTASI BERBEDA (Physicochemical Properties of Cellulose Produced by Bacterial Isolate Gluconacetobacter xylinus KRE-65 in Different Fermentation Methods)

2015 ◽  
Vol 35 (04) ◽  
pp. 434
Author(s):  
Sarkono Sarkono ◽  
Sukarti Moeljopawiro ◽  
Bambang Setiaji ◽  
Langkah Sembiring
Keyword(s):  
Physicochemical Properties ◽  
Surface Structure ◽  
Bacterial Cellulose ◽  
Spherical Shape ◽  
Dry Weight ◽  
Xrd Analysis ◽  
Cellulose Microfibrils ◽  
Degree Of Crystallinity ◽  
Gluconacetobacter Xylinus ◽  
Fermentation Method

Physicochemical properties of cellulose produced by local bacterial strain Gluconacetobacter xylinus KRE-65 by static and agitated fermentation methods was studied. Cellulose production by G. xylinus KRE-65 was carried out in coconut base medium with static and agitated fermentation methods. The dry weight, morphological and physicochemicalproperties of bacterial cellulose were compared based on SEM, XRD and FTIR analyses. The results showed that the G. xylinus KRE 65 in the static fermentation produced cellulose higher than agitated fermentation. Static fermentation method produced bacterial cellulose in the sheets form, while agitated fermentation produced fragmented cellulose with predominantly spherical shape. The observation of the surface structure of bacterial cellulose by SEM showed that the static fermentation method generated woven densely cellulose microfibrils, while agitated fermentation significantly changed the surface structure, namely woven microfibrils become more loose with formed larger and higher number of pores. The degree of crystallinity of bacterial cellulose by XRD analysis in static fermentation was 91%, agitated fermentation at 100 rpm was 73% and agitated fermentation at 150 rpm was 72%. FTIR spectra indicated that the pellicles produced by G. xylinus KRE 65 with static and agitated fermentation were found as cellulose. Cellulose produced from both fermentation methods showed different physicochemical properties, therefore they can be applied for different purposes in accordingly.Keywords: Gluconacetobacter xylinus, bacterial cellulose, static fermentation, agitated fermentation ABSTRAKSifat fisikokimiawi selulosa yang dihasilkan oleh strain lokal bakteri Gluconacetobacter xylinus KRE-65 dengan metode fermentasi statis dan agitatif telah diteliti. Produksi selulosa oleh G. xylinus KRE-65 dilakukan dalam media dasar air kelapa dengan metode fermentasi statis dan agitatif. Selulosa yang dihasilkan selanjutnya dibandingkan beratkering, bentuk morfologi dan sifat fisikokimiawinya menggunakan metode SEM, XRD dan FTIR. Hasil penelitian menunjukkan bahwa G. xylinus KRE 65 menghasilkan selulosa lebih tinggi pada metode fermentasi statis dibandingkan fermentasi agitatif. Metode fermentasi statis menghasilkan selulosa bakteri yang berbentuk lembaran sedangkan fermentasi agitatif menghasilkan selulosa yang terpecah-pecah dengan bentuk dominan bulat. Pengamatan struktur permukaan selulosa bakteri dengan SEM memperlihatkan bahwa metode fermentasi statis menghasilkan selulosadengan anyaman mikrofibril yang padat, sedangkan fermentasi agitatif menyebabkan terjadinya perubahan struktur permukaan yaitu melonggarnya anyaman mikrofibril dan terbentuknya pori-pori yang lebih besar dan lebih banyak. Derajat kristalinitas selulosa bakteri dengan analisis XRD pada metode fermentasi statis sebesar 91%, fermentasiagitatif 100 rpm sebesar 73% dan fermentasi 150 rpm sebesar 72%. Spektra FTIR mengindikasikan bahwa pelikel yang dihasilkan oleh G. xylinus KRE 65 pada kedua metode fermentasi tersebut merupakan selulosa. Selulosa yang dihasilkan dari fermentasi statis dan agitatif mempunyai sifat fisikokimiawi yang berbeda sehingga dapat diterapkandalam aplikasi yang berbeda sesuai dengan sifat fisikokimiawi yang dibutuhkan.Kata kunci: Gluconacetobacter xylinus, selulosa bakteri, fermentasi statis, fermentasi agitatif

Effect of carbon sources on physicochemical properties of bacterial cellulose produced from Gluconacetobacter xylinus MTCC 7795

e-Polymers ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 331-336
Author(s):  
Rushali Singh ◽  
Ashwani Mathur ◽  
Navendu Goswami ◽  
Garima Mathur
Keyword(s):  
Physicochemical Properties ◽  
Bacterial Cellulose ◽  
Specific Growth ◽  
Carbon Sources ◽  
Xrd Analysis ◽  
Culture Conditions ◽  
Shake Flask Culture ◽  
Gluconacetobacter Xylinus ◽  
X Ray ◽  
First Time

AbstractIn this study, the effect of modified Hestrin Schramm (HS) medium supplemented with different carbon sources viz., glucose, fructose, galactose and lactic acid on the yield and physicochemical properties of bacterial cellulose (BC) produced from Gluconacetobacter xylinus strain MTCC 7795 in shake flask culture conditions was investigated. Growth studies indicated that all carbon sources supported the growth of bacteria, though specific growth rate and doubling time differs. Fructose gave the highest cellulose yield of 7.72 mg/ml after 130 h of fermentation, while yield in glucose and galactose supplemented medium were 4.49 mg/ml and 3.38 mg/ml, respectively. X-ray powder diffraction (XRD) analysis revealed that all BC samples were amorphous in comparison to commercial cellulose. Fourier transform infrared (FTIR) spectroscopic investigations of bacterial cellulose (BC) samples affirm the purity of the cellulose produced. No significant variations in physicochemical properties of cellulose samples produced with different carbon sources were observed. This study for the first time has investigated the effect of carbon sources on physicochemical properties of bacterial cellulose produced by G. xylinus MTCC 7795 and provides a strategy for economical production of BC with anticipated application in therapeutics and tissue engineering.

What are the ranges and basis of auxeticity in the phases of cellulose microfibrils?

2017 ◽  
Author(s):  
Akwasi Asamoah
Keyword(s):  
Bacterial Cellulose ◽  
Raman Spectra ◽  
Laser Power ◽  
Microfibrillated Cellulose ◽  
Glass Slide ◽  
Flax Fibre ◽  
Cellulose Microfibrils

<p>One sample of 1D bundle of cellulose microfibrils in the form of lignified flax fibre (0.10526 mm x 10 mm), and one 2D networks of cellulose microfibrils in the form of tunicate cellulose (0.07 mm x 5 mm x 10 mm), bacterial cellulose (0.135 mm x 5 mm x 10 mm) and microfibrillated cellulose (0.08 mm x 5 mm x 10 mm) were put on a glass slide parallel to the principal spectrometer axis. Raman spectra were measured all round in-plane under both half (in 5° steps) polarisation from 0° to 360° in extended mode between 100 cm<sup>-1</sup> and 1150 cm<sup>-1</sup> in 3 accumulations at 10s exposure and 100% laser power. The cursor was placed at the peak of the 1095 cm<sup>-1</sup> band, and intensity read.</p>

scholarly journals Characterizing Bacterial Cellulose Produced byKomagataeibacter sucrofermentans H-110 on Molasses Medium and Obtaining a Biocomposite Based on It for the Adsorption of Fluoride

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1422
Author(s):  
Viktor V. Revin ◽  
Alexander V. Dolganov ◽  
Elena V. Liyaskina ◽  
Natalia B. Nazarova ◽  
Anastasia V. Balandina ◽  
...  
Keyword(s):  
Composite Material ◽  
Adsorption Capacity ◽  
Bacterial Cellulose ◽  
Functional Materials ◽  
Degree Of Crystallinity ◽  
Maximum Adsorption Capacity ◽  
Fluoride Ions ◽  
Sorption Ability ◽  
Wide Range ◽  
Biocomposite Material

Currently, there is an increased demand for biodegradable materials in society due to growing environmental problems. Special attention is paid to bacterial cellulose, which, due to its unique properties, has great prospects for obtaining functional materials for a wide range of applications, including adsorbents. In this regard, the aim of this study was to obtain a biocomposite material with adsorption properties in relation to fluoride ions based on bacterial cellulose using a highly productive strain of Komagataeibacter sucrofermentans H-110 on molasses medium. Films of bacterial cellulose were obtained. Their structure and properties were investigated by FTIR spectroscopy, NMR, atomic force microscopy, scanning electron microscopy, and X-ray structural analysis. The results show that the fiber thickness of the bacterial cellulose formed by the K. sucrofermentans H-110 strain on molasses medium was 60–90 nm. The degree of crystallinity of bacterial cellulose formed on the medium was higher than on standard Hestrin and Schramm medium and amounted to 83.02%. A new biocomposite material was obtained based on bacterial cellulose chemically immobilized on its surface using atomic-layer deposition of nanosized aluminum oxide films. The composite material has high sorption ability to remove fluoride ions from an aqueous medium. The maximum adsorption capacity of the composite is 80.1 mg/g (F/composite). The obtained composite material has the highest adsorption capacity of fluoride from water in comparison with other sorbents. The results prove the potential of bacterial cellulose-based biocomposites as highly effective sorbents for fluoride.

scholarly journals Effect of cross-linkable bacterial cellulose nanocrystals on the physicochemical properties of silk sericin films

2021 ◽  
Vol 97 ◽  
pp. 107161
Author(s):  
Jeongmin Nam ◽  
Yujin Hyun ◽  
Subin Oh ◽  
Jinseok Park ◽  
Hyoung-Joon Jin ◽  
...  
Keyword(s):  
Physicochemical Properties ◽  
Bacterial Cellulose ◽  
Cellulose Nanocrystals ◽  
Silk Sericin

scholarly journals Effect of Gluconacetobacter xylinus cultivation conditions on the selected properties of bacterial cellulose

2016 ◽  
Vol 18 (4) ◽  
pp. 117-123 ◽  
Author(s):  
Karol Fijałkowski ◽  
Anna Żywicka ◽  
Radosław Drozd ◽  
Marian Kordas ◽  
Rafał Rakoczy
Keyword(s):  
Bacterial Cellulose ◽  
Bacterial Strain ◽  
Point Of View ◽  
Synthesis Process ◽  
Cellulose Synthesis ◽  
Gluconacetobacter Xylinus ◽  
Water Release ◽  
Good Efficiency ◽  
Cellulose Fibrils ◽  
Culturing Conditions

Abstract The aim of the study was to analyze the changes in the parameters of bacterial cultures and bacterial cellulose (BC) synthesized by four reference strains of Gluconacetobacter xylinus during 31-day cultivation in stationary conditions. The study showed that the most visible changes in the analyzed parameters of BC, regardless of the bacterial strain used for their synthesis, were observed in the first 10–14 days of the experiment. It was also revealed, that among parameters showing dependence associated with the particular bacterial strain were the rate and period of BC synthesis, the growth rate of bacteria anchored to the cellulose fibrils, the capacity to absorb water and the water release rate. The results presented in this work may be useful in the selection of optimum culturing conditions and period from the point of view of good efficiency of the cellulose synthesis process.

scholarly journals Bacterial Cellulose as a Substrate for Microbial Cell Culture

2014 ◽  
Vol 80 (6) ◽  
pp. 1926-1932 ◽  
Author(s):  
Na Yin ◽  
Thiago M. A. Santos ◽  
George K. Auer ◽  
John A. Crooks ◽  
Piercen M. Oliver ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Physicochemical Properties ◽  
Stationary Phase ◽  
Bacterial Cellulose ◽  
Growth Rates ◽  
Bacterial Cell ◽  
Acetobacter Xylinum ◽  
Primary Factor ◽  
Nutrient Diffusion

ABSTRACTBacterial cellulose (BC) has a range of structural and physicochemical properties that make it a particularly useful material for the culture of bacteria. We studied the growth of 14 genera of bacteria on BC substrates produced byAcetobacter xylinumand compared the results to growth on the commercially available biopolymers agar, gellan, and xanthan. We demonstrate that BC produces rates of bacterial cell growth that typically exceed those on the commercial biopolymers and yields cultures with higher titers of cells at stationary phase. The morphology of the cells did not change during growth on BC. The rates of nutrient diffusion in BC being higher than those in other biopolymers is likely a primary factor that leads to higher growth rates. Collectively, our results suggest that the use of BC may open new avenues in microbiology by facilitating bacterial cell culture and isolation.

scholarly journals SYNTHESIS OF BENTONITE NANOCLAY AND INCORPORATION OF CASSIA FISTULA LEAF EXTRACT TO FORM ORGANOBENTONITE: CHARACTERIZATION AND ITS BIOMEDICAL APPLICATIONS

2018 ◽  
Vol 11 (9) ◽  
pp. 381
Author(s):  
Anand Raj Lfa ◽  
Jeslin J
Keyword(s):  
Biomedical Applications ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Antioxidant Property ◽  
Clot Lysis ◽  
Cassia Fistula ◽  
Thrombolytic Activity ◽  
Lysis Activity

Objective: In this work, methanolic leaf extract from Cassia fistula (known as aragvadha) was incorporated into bentonite nanoclay to form organobentonite. This organobentonite of nanosize was further used for its effective biomedical applications since medicinal clay finds its own advantage over decades.Methods: The bentonite nanoclay was produced by energetic stirring followed by centrifugation and was characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared (FTIR). The organobentonite was produced using freeze and thaw method. Antioxidant property was studied using Molyneux method, and thrombolytic activity was analyzed using in vitro clot lysis method.Results: The nanosize of bentonite nanoclay between 57 and 82 nm with irregular to spherical shape was confirmed using SEM analysis. The sharp diffraction peak in XRD analysis shows the crystalline nature of bentonite nanoclay, and FTIR results revealed the successful incorporation of the methanolic extract within the bentonite nanoclay. The organobentonite exhibits 84.5% antioxidant property as well as 31% clot lysis activity when compared to the extract and the bentonite nanoclay.Conclusion: Thus, the non-toxic and inexpensive bentonite nanoclay have a high aspect ratio with multifarious applications in medicine, food, cosmetics, and health products. Through this study, the bentonite nanoclay modified using plant alkaloid (organobentonite) is found to possess good biomedical property.

scholarly journals Gamma Ray-Induced Synthesis of Silver Nanoparticles using Bacterial Cellulose as a Multi-Functional Agent and its Application

2021 ◽  
Author(s):  
Nasser H. Mohammad ◽  
gamal Mohamed elsherbiny ◽  
Ali A. Hammad ◽  
Ahmed A. Askar ◽  
Salwa A. Abou El Nour
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Cellulose ◽  
Food Packaging ◽  
Gamma Ray ◽  
Color Change ◽  
Spherical Shape ◽  
Inhibition Zone ◽  
Food Samples ◽  
One Step ◽  
Novel Method

Abstract Antibacterial coatings based on bacterial cellulose (BC) have been widely used in many fields including food packaging and wound dressing. In this study, we aimed to synthesis of colloidal AgNPs and BC/ AgNP composite by using BC as a reducing and capping agent in one step reaction induced by gamma-ray. Bacterial strain Komagataeibacter rhaeticus N1 MW322708 was used for biosynthesis BC by inoculation on Hestrin and Schramm medium and incubated statically at 35 °C for 10 days. BC sheet was formed, harvested, purified, and dried, then used for the synthesis of AgNPs and BC/AgNP by soaked 0.05 g of dried BC in 10ml of 1mM aqueous AgNO3 solution for 2h and then irradiated by gamma-ray under different doses. Color change from yellow to deep brown indicated the synthesis of AgNPs and BC/AgNP. The optical spectra of synthesized AgNPs revealed that the surface plasmon resonance was localized around 420 nm. DLS analysis showed that the mean diameter of AgNPs was 49.5 nm with a -19.36-mV value of zeta potential. TEM images revealed the spherical shape of synthesized AgNPs. The results of FESEM, FTIR, and XRD confirmed the formation of BC/AgNO3 composite. The highly crystalline nature of the BC membrane and BC/AgNP composite was observed in XRD measurements with a crystal size of 5.416 and 5.409 nm, respectively. The antibacterial activity of BC and BC/AgNP against pathogenic bacterial isolated from Pastirma food samples revealed that BC does not show antibacterial activity, while BC/AgNP composite showed antibacterial potency against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Proteus mirabilis, and Escherichia coli, with an inhibition zone of (mm) 9±1, 9±0.57, 10±1.15, 8±0.5 and 7±0.28, respectively. We concluded that this novel method presented in this paper offers a promising route for both AgNPs and BC/AgNP composites synthesis using a green, renewable biopolymer as a multifunctional agent and potential to be applied in the future development of food packing, biomedical instruments, and therapeutics.

Specifics of Microstructure and Phase Composition of High-Speed Steel R6M5

2013 ◽  
Vol 404 ◽  
pp. 20-24 ◽  
Author(s):  
Маzhyn Skakov ◽  
Bauyrzhan Rakhadilov ◽  
Gaukhar Karipbayeva
Keyword(s):  
Phase Composition ◽  
High Speed ◽  
Volume Fraction ◽  
Electron Backscatter Diffraction ◽  
High Speed Steel ◽  
Spherical Shape ◽  
Xrd Analysis ◽  
Steel R6m5 ◽  
Solid Carbide ◽  
Backscatter Diffraction

In this paper microstructure, morphology, elemental composition, phase composition and crystal structure of the sample steel R6M5 were investigate by using the methods of scanning electron microscopy, electron backscatter diffraction (EBSD) analysis and X-ray diffraction (XRD) analysis. Determined that the microstructure of steel R6M5 after hardening and three-time tempering consists of tempered martensite and solid carbide M6C and MC-type with spherical shape and a diameter of less than 3 μm. Detected that the volume fraction of each carbide amounted to 10.4±0.6% and 2.3±0.4% - for grey and bright carbides, respectively, and that the sizes of bright carbides particles in the microstructure of steel R6M5 are 0.4-4,5 μm, and the sizes of grey carbides particles are 0.5-1.1 μm. XRD analysis showed that the main carbides in the studied steel are carbides М6С and MC, which have complicated the FCC crystal lattice and the Fd3m spatial group. Determined that carbides are uniform and monocrystalline. ESBD analysis with the support of the XRD analysis showed that carbides spherical shape М6С fit to Fe3W3C composition.

Sign in / Sign up

Export Citation Format

Share Document