Identification of the Effect of Sulfur Spray on Okra (AbelmoschusesculentusL. var. MI 5)Seedlings

2016 ◽  
Vol 3 (4) ◽  
Author(s):  
NILANTHI DAHANAYAKE ◽  
DDPBD DEHIGASPITIYA ◽  
AG MAYURI DHANANJANIE ◽  
AGKMWS ATAPATTU ◽  
PCD PERERA

The study investigated that the effect of sulfur solutions as foliar spray with different concentrations on some selected growth parameters of ladies fingers plants. Pot experiment was conducted with six treatments (2g/L, 4g/L, 6g/L, 8g/L and 10g/L sulfur solutions) and three replicates. For each pot,two plants were maintained throughout the experiment. After seedling emerging, sulfur solutions were sprayed in every week using OSATU sprayer (5 L), until all the leaves got wet. After 30 days physiological features (Number of leaves, leaf length andleaf width) were recorded. Pest and disease incident also recorded at the mean time. Completely Randomized Design (CRD) with five replicates was used. Statistical analysis was performed with Duncan’s multiple range test using SAS software (version 9.1.3). Result showed that the highest significant values in number of leaves (26), leaf length (21cm) and leaf width (24cm) were observed that in 10g/L solution of sulfur applied as foliar spray. With the increasing concentration of sulfur solutions the values for the selected physiological features also increased. Pest and disease incidence was significantly higher in control experiment while all the other treated plants don’t showed any physiological damage or symptom. The results revealed that the application of sulfur as a foliar spray can be effectively use in increase the net photosynthetic area and to reduce the pest and disease incidence.

2015 ◽  
Vol 46 (4) ◽  
pp. 590-592 ◽  
Author(s):  
Cibele Mantovani ◽  
Kathia Fernandes Lopes Pivetta

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.


Author(s):  
J. G. Crispim ◽  
M. M. Rêgo ◽  
E. R. Rêgo ◽  
Â. M. S. Pessoa ◽  
P. A. Barroso

<p>Este trabalho teve por objetivo avaliar o desenvolvimento de clones de <em>Pyrostegia venusta </em>em diferentes tipos de substratos. O experimento foi conduzido em delineamento inteiramente casualizado, com 06 tratamentos e 10 repetições, cada repetição foi constituída por um tubete de polietileno com capacidade de 103cm<sup>3</sup>. Os tratamentos testados foram: (T1) 100% de areia lavada; (T2) 50% areia lavada + 50% substrato comercial Plantmax®; (T3) 100% de substrato comercial Plantmax®; (T4) 50% areia lavada + 50% de esterco bovino; (T5) 1/3 areia lavada + 1/3 esterco bovino +1/3 de Plantmax® e (T6) 50% esterco bovino + 50% de Areia lavada. Após 30 dias foram avaliados o número de estacas sobreviventes, comprimento do broto (cm), número de folhas, comprimento da folha (cm), número de raízes e comprimento da raiz principal (cm).  Os dados foram submetidos a análise de variância e as médias comparadas pelo teste de Tukey a 5% de probabilidade. Por meio da análise de variância (p&lt;0,01), detectou-se significância estatística, para as variáveis, sobrevivência das estacas e número de brotos por estaca de <em>P. venusta</em>, quando cultivadas em substrato composto por 50% areia lavada + 50% substrato comercial Plantmax®. Essa mistura apresenta a vantagem de ser mais econômica quando comparado ao substrato comercial Plantmax®, sendo portanto, recomendada na produção de mudas de <em>cipó-de-São João</em><em>.</em></p><p align="center"><strong><em>Utilization of different substrates in the propagation of Pyrostegia venusta by cuttings</em></strong></p><p><strong>Abstract</strong><strong>: </strong>The experiment was carried out in a completely randomized design with 06 treatments and 10 replicates, each replicate consisted of polyethylene plastic tube the size of 103cm<sup>3</sup>. The treatments were: (T1) 100% washed sand; (T2) 50% washed sand + 50% commercial substrate Plantmax®; (T3) 100% substrate commercial Plantmax®; (T4) 50 % washed sand + 50% cattle manure; (T5) 1/3 washed sand + 1/3 manure +1/3 Plantmax® and Plantmax (T6) 50 % cattle manure + 50% washed sand. After 30 days, were evaluated number of cutting survival, bud length in cm, number of leaves, leaf length in cm, number of roots and length of the main root. Data were submitted to analysis of variance and means were compared by Tukey test at 5% probability. By analysis of variance (p&lt; 0.01), there was significant differences for the variables, czutting survival and number of shoots per cutting when cultured on substrate composed of 50% washed sand + 50% commercial substrate Plantmax®. Furthermore, this mixture has the advantage to be cheaper as compared to substrate Plantmax®, so we recommend the seedlings of Saint John’s vine.</p><p><em><br /></em></p>


2013 ◽  
Vol 13 (1) ◽  
pp. 10-18
Author(s):  
Djoko Eko Hadi Susilo ◽  
Sri Handayani Wahyuningsih

This study aims to determine the growth response of the love wave plant (Anthurium plowmanii) on Petrovita leaf fertilizer in an advanced nursery conducted in August - October 2011 in Panarung Village, Pahandut District, Palangka Raya City. Single treatment design (one factor) with 10 (ten) repetitions in the form of Petrovita leaf fertilizer (D) consisting of 5 concentration levels, namely: d1 = 0.5 ml l-1 (0.05%); d2 = 1.0 ml l-1 (0.10%); d3 = 1.5 ml l-1 (0.15%); d4 = 2.0 ml l-1 (0.20%); and d5 = 2.5 ml l-1 (0.25%) using a completely randomized design. Observations were made when the plants in the advanced nurseries had received treatment applications 4 times at intervals of 2 weeks. Observations were made on plant height (cm); stem height (cm); number of leaves (leaves); leaf length (cm); leaf width (cm); and leaf area (cm2). The results of this study indicate that Petrovita complete leaf fertilizer has a very significant effect on increasing the growth of love wave plants aged 8 MST in advanced nurseries. Petrovita fertilizer concentration of 0.2% or 2 ml l-1 (d4) is the best and most efficient treatment for love wave plants in advanced nurseries. It is recommended to increase the growth of love wave plants in advanced nurseries using Petrovita 0.2% concentration but tried with an interval of less than 2 weeks (for example once a week).


Author(s):  
Primavera Pérez-Ramos ◽  
Yuri Villegas-Aparicio ◽  
Rigoberto Castro-Rivera ◽  
Ernesto Castañeda-Hidalgo ◽  
Aarón Martínez-Gutiérrez ◽  
...  

Objective: The objective of this study was to evaluate the response of different concentrations of a fungal consortium on the growth and yield of Cenchrus purpureus ecotypes under storm conditions.Design/Methodology/Approach: An experiment was established under a completely randomized design with a 9X2X4 factorial arrangement. The factors were 9 ecotypes of Cenchrus purpureus, two seasons of the year (Summer-Fall and Winter-Spring), and four levels of mycorrhizal consortium. The variables evaluated were: number of buds, number of leaves, height of the bud, leaf length, total biomass, and leaf-stalk ratio.Results: The response of the ecotypes had a differential effect on the variables NB, LLL, TB and L/SR, while the season affected the variables LN, PH, LLL, MLL, SLL, TB and L/SR. Low inoculation levels increased the variables NB, PH, LLL, MLL and TB.Study Limitations/Implications: The evaluated factors indicate that the level of inoculant and the season determine the growth and yield of Cenchrus purpureus.Findings/Conclusions: The mycorrhizal consortium dose and season of the year mainly determined the growth and yield of C. purpureus. Ecotypes respond differently to changes in the season and in mycorrhizal consortium dose. The evaluated factors indicate that the mycorrhiza dose and the season of the year determine the growth and yield of C.purpureus. Two growth strategies of the ecotypes are visualized: 1) many buds, with few small leaves and 2) few buds with many large leaves


Author(s):  
Subandi M ◽  
Arkhan Jannata ◽  
Sofiya Hasani

The propagation of Cavendish (Musa acuminata L.) seedling conventionally relatively took a longer time, not uniformed in quality and possibly has a disease that is derived from unhealthy mother stock. This research aimed to find the effect of IBA and BA on difference concentrations on the growth of plantlet, also to determine the optimum concentration of IBA and BA on the growth of Cavendish bud plantlets in vitro. This research had been carried out started from April to July 2018 in Tissue Culture Laboratory, Research Institute of Horticultural Seed Development of Pasir Banteng, Sumedang, West Java. Indonesia. The method used in this research was Completely Randomized Design with two factors. The first factor was IBA (i0 = 0 mg L-1, i1= 0,75 mg L-1, dan i2= 1,5 mg L1), and the second factor was BA (b1= 1 mg L-1 b2= 3 mg L-1 b3= 5 mg L-1 b4= 7 mg L-1), replicated three times. Duncan Multiple Range Test (DMRT) was used as a further test. The result showed an interaction between IBA and BA on the parameters of shoot growth and the number of leaves at 6 WAC. IBA showed an independent effect on the number of buds. The addition of 1,5 mg L-1 IBA and 3 mg L-1 BA was optimum for the growth of Cavendish bud explants.


2021 ◽  
Vol 6 (2) ◽  
pp. 124-130
Author(s):  
Rasyid Abdulaziz ◽  
Sri Widodo Agung Suedy ◽  
Munifatul Izzati

Selasih (Ocimum basilicum L.) memiliki kandungan minyak atsiri yang dapat digunakan dalam industri kosmetik, parfum, dan medis. Penelitian ini bertujuan mengetahui hubungan usia panen dengan biomassa dan produksi minyak atsiri pada organ daun serta batang selasih. Penelitian menggunakan Rancangan Acak Lengkap (RAL) pola faktorial, dengan faktor pertama usia panen (1; 1,5; dan 2 bulan), dan faktor kedua organ tanaman (daun dan batang). Media tanam menggunakan tanah dan kompos (1:1) yang dimasukkan dalam polibag ukuran 30cm x 30cm, dan diberi naungan paranet 25%. Parameter yang diamati: data pertumbuhan (tinggi tanaman, jumlah daun dan cabang primer), biomassa, dan produksi minyak atsiri. Analisis data menggunakan Anaylysis of Variance (ANOVA) dan Duncan's Multiple Range Test(DMRT) pada taraf kepercayaan 95 %. Penelitian menunjukkan hasil bahwa tanaman yang dipanen pada umur lebih tua menunjukkan pertumbuhan, biomassa dan produksi minyak atsiri yang lebih tinggi. Pada usia 1,5 bulan, biomassa meningkat 114,485% dibanding usia 1 bulan, sedangkan pada usia 2 bulan, peningkatan biomasa 91,410%  dibanding usia1,5 bulan. Produksi minyak atsiri tertinggi dihasilkan oleh organ daun pada usia panen 2 bulan sebesar 0,273g, dan 0,023g pada organ batang. Kesimpulan penelitian ini menunjukkan bahwa usia panen berbeda berpengaruh nyata terhadap biomassa dan produksi minyak atsiri tanaman selasih. Basil (Ocimum basilicum L.) contains essential oils that can be used in the cosmetic, perfume, medical industries. This study aims to determine the relationship between harvest age and biomass and essential oil production in basil leaves and stems. The study used a completely randomized design (CRD) with a factorial pattern, with the first factor being harvest age (1; 1.5; and 2 months), and the second factor being plant organs (leaves and stems). The planting medium used soil and compost (1:1) which was put in 30cm x 30cm polybags and was given a 25% para net shade. Parameters observed: growth data (plant height, number of leaves, and primary branches), biomass, and essential oil production. Data analysis used Analysis of Variance (ANOVA) and Duncan's Multiple Range Test (DMRT) at a 95% confidence level. Research shows that plants harvested at an older age show higher growth, biomass, and essential oil production. At the age of 1.5 months, biomass increased by 114.485% compared to the age of 1 month, while at the age of 2 months, the increase in biomass was 91.410% compared to the age of 1.5 months. The highest essential oil production was produced by leaf organs at 2 months of harvesting at 0.273g, and 0.023g in stem organs. The conclusion of this study showed that different harvest ages had a significant effect on the biomass and essential oil production of basil plants.


2018 ◽  
Vol 9 (2) ◽  
pp. 142-153
Author(s):  
Edna Maria Bonfim-Silva ◽  
Juliana Tertezinha Sasso Paludo ◽  
Tonny José Araújo da Silva ◽  
William Fenner ◽  
Maurício Dutra Zanotto

It is essential to have knowledge of changes in soil structure, as a result of agricultural use and its relationship with crop development, especially those with potential for use in new regions, such as the cultivation of safflower (Carthamus tinctorius L.) in the Cerrado. In view of this dynamics, the objective was to evaluate the phytometric components of safflower genotypes submitted to levels of soil densities in Cerrado Oxisol. Ten safflower genotypes were then submitted to compaction (five densities: 1.0, 1, 2, 1.4, and 1.8 Mg m-3) in a completely randomized design with four replications under greenhouse conditions. The plots consisted of an overlap of three rings of polyvinylidene chloride of 0.01 m height each, and the treatments were applied in the intermediate layer. The phytometric components (plant height, number of leaves and stem diameter) and shoot dry mass of the crop were evaluated at 30, 60 and 90 days after sowing. There was no significant interaction between safflower genotypes and soil densities, with the effects being isolated for genotypes and/or densities when significant. For the phytometric characteristics evaluated, the genotypes PI250196, PI301049, PI305173, and PI305205 are more stable about the variations of soil density in comparison to the others. The phytometric components and dry mass of safflower shoot are impaired from the mean density of 1.2 Mg m-3.


2015 ◽  
Vol 61 (3) ◽  
pp. 13-22 ◽  
Author(s):  
Shamila Yadolahi Zadeh ◽  
Ali Akbar Ramin ◽  
Bahram Baninasab

SummaryThis study was conducted in order to determine the appropriate treatment for breaking dormancy and the effect of salinity on seed germination of purple coneflower (Echinacea purpureacv. Magnus), in two separate experiments. In the first experiment, five levels of gibberellic acid (GA3) (0, 250, 500, 1000, and 1500 mg×L−1) with four levels of cold moist stratification period of seeds at 5°C (0, 5, 10 and 15 days) were launched. A factorial experiment was conducted in a completely randomized design with four replications. The statistical analysis showed that concentration of 250 mg×L−1GA3with 10 days of cold moist chilling significantly increased the percentage of germination of normal seedlings and reduced the mean time of germination. In the second experiment, the seeds were chilled for 10 days at 5°C and half of them treated with 250 mg×L−1GA3for 24 hours. The seeds treated with GA3, and those non-treated were subjected to NaCl for salinity stress. The experiment was conducted using five salinity levels (0, 20, 40, 60 and 80 mM NaCl) in four replications in a completely randomized design. The results showed that purple coneflower is highly sensitive to salinity in the germination stage. The results also showed that by increasing salinity levels, the percentage of germination and normal seedlings significantly decreased and the mean time to germination increased, compared to the control treatment. But the seeds treated with GA3showed higher viability and better performance under salinity stress condition.


2015 ◽  
Vol 52 (3) ◽  
pp. 405-417 ◽  
Author(s):  
E. AWOLOGBI ◽  
E. I. HAMADINA

SUMMARYProlonged inability of yam tubers to sprout/germinate is associated with the expression of tuber dormancy, and the start of dormancy in tubers is linked to the onset of tuber formation. Although the plant hormone, abscisic acid (ABA) is known to regulate the onset and maintenance of dormancy in tubers, there are no known method(s) of inducing shoot growth (sprouting) on seed yam tubers that are harvested at 240 days after vine emergence or earlier. Therefore, this study was conducted to: (1) determine whether the absorption of fluridone, a reputed ABA inhibitor, prior to or during early seed yam tuber formation would induce sprouting, and (2) determine the effect of fluridone on early shoot growth. The study was a 2×4 factorial experiment arranged in a Completely Randomized Design (CRD) with eight treatment combinations replicated three times. Two species of yam with long dormancy durations were used: D. rotundata var TDr 131 and D. alata var TDa 98/01166; and three concentrations of fluridone were tested (30, 50, 100 µM). Young plants (69 days after vine emergence) of TDr 131 and TDa 98/01166 were grown in a coco coir medium hydroponics system using Hoagland's Nutrient Solution, with or without the test fluridone concentrations. In all fluridone treatments, most leaves of both species of yam had 30–90% of their surface bleached while the stems appeared purplish. In both Controls, all the newly formed seed tubers that were harvested at 90 and 104 days after vine emergence were dormant (devoid of any new shoots/sprouts). In contrast, over 70% of the seed tubers that developed in 30 and 100 µM fluridone had at least one new shoot, particularly in TDa 98/01166. The fluridone treatments did not significantly affect tuber weight or vegetative growth parameters like dimensions and number of leaves. This study has shown that the duration from vine emergence of the old tuber to sprouting of the new tuber can be reduced by more than half if young plants absorbed fluridone during early tuber development.


2020 ◽  
Vol 12 (1) ◽  
pp. 43-49
Author(s):  
Verena Agustini ◽  
Irma Rahayu ◽  
Leonardo A. Numberi ◽  
Ziyadatun Ni’mah

The effect of chitosan  on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm  and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan  had a positive influence on the growth and development of orchids D. lasianthera plantlet  in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.


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