scholarly journals Development of A Novel Gene Expression System for Secretory Production of Heterologous Proteins via the General Secretory (Sec) Pathway in Corynebacterium glutamicum

2018 ◽  
Vol 16 (1) ◽  
pp. 42-48 ◽  
Author(s):  
Huimin Jia ◽  
Hedan Li ◽  
Lirong Zhang ◽  
Daqing Xu
Keyword(s):  
Gene Expression ◽  
Corynebacterium Glutamicum ◽  
Expression System ◽  
Heterologous Proteins ◽  
Gene Expression System ◽  
Sec Pathway ◽  
Novel Gene ◽  
Secretory Production

scholarly journals A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector

2013 ◽  
Vol 31 (3) ◽  
pp. 1089-1095 ◽  
Author(s):  
MASAMI WATANABE ◽  
MASAKIYO SAKAGUCHI ◽  
RIE KINOSHITA ◽  
HARUKI KAKU ◽  
YUICHI ARIYOSHI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adenoviral Vector ◽  
Expression System ◽  
Gene Expression System ◽  
Novel Gene ◽  
Anticancer Effects

scholarly journals 153. A Novel Gene Expression System Using Human Artificial Chromosome-HSV-1 Vectors

2006 ◽  
Vol 13 ◽  
pp. S60
Author(s):  
Daniela Moralli ◽  
Kirsty Simpson ◽  
Richard Wade-Martins ◽  
Zoia Larin Monaco
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Artificial Chromosome ◽  
Human Artificial Chromosome ◽  
Gene Expression System ◽  
Novel Gene ◽  
Hsv 1

scholarly journals A novel gene expression system for Ralstonia eutropha based on the T7 promoter

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Muzi Hu ◽  
Bin Xiong ◽  
Zhongkang Li ◽  
Li Liu ◽  
Siwei Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ralstonia Eutropha ◽  
Expression System ◽  
T7 Promoter ◽  
Gene Expression System ◽  
Novel Gene

scholarly journals Development of a Hyperosmotic Stress Inducible Gene Expression System by Engineering the MtrA/MtrB-Dependent NCgl1418 Promoter in Corynebacterium glutamicum

2021 ◽  
Vol 12 ◽  
Author(s):  
Jingwen Huang ◽  
Jiuzhou Chen ◽  
Yu Wang ◽  
Tuo Shi ◽  
Xiaomeng Ni ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Engineering ◽  
Corynebacterium Glutamicum ◽  
Expression System ◽  
Regulation Of Gene Expression ◽  
Hyperosmotic Stress ◽  
Inducible Gene Expression ◽  
Gene Expression System ◽  
Inducible Promoters ◽  
Inducible Gene

Corynebacterium glutamicum is an important workhorse for industrial production of diversiform bioproducts. Precise regulation of gene expression is crucial for metabolic balance and enhancing production of target molecules. Auto-inducible promoters, which can be activated without expensive inducers, are ideal regulatory tools for industrial-scale application. However, few auto-inducible promoters have been identified and applied in C. glutamicum. Here, a hyperosmotic stress inducible gene expression system was developed and used for metabolic engineering of C. glutamicum. The promoter of NCgl1418 (PNCgl1418) that was activated by the two-component signal transduction system MtrA/MtrB was found to exhibit a high inducibility under hyperosmotic stress conditions. A synthetic promoter library was then constructed by randomizing the flanking and space regions of PNCgl1418, and mutant promoters exhibiting high strength were isolated via fluorescence activated cell sorting (FACS)-based high-throughput screening. The hyperosmotic stress inducible gene expression system was applied to regulate the expression of lysE encoding a lysine exporter and repress four genes involved in lysine biosynthesis (gltA, pck, pgi, and hom) by CRISPR interference, which increased the lysine titer by 64.7% (from 17.0 to 28.0 g/L) in bioreactors. The hyperosmotic stress inducible gene expression system developed here is a simple and effective tool for gene auto-regulation in C. glutamicum and holds promise for metabolic engineering of C. glutamicum to produce valuable chemicals and fuels.

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