scholarly journals 153. A Novel Gene Expression System Using Human Artificial Chromosome-HSV-1 Vectors

2006 ◽  
Vol 13 ◽  
pp. S60
Author(s):  
Daniela Moralli ◽  
Kirsty Simpson ◽  
Richard Wade-Martins ◽  
Zoia Larin Monaco
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Artificial Chromosome ◽  
Human Artificial Chromosome ◽  
Gene Expression System ◽  
Novel Gene ◽  
Hsv 1

scholarly journals A novel gene expression system strongly enhances the anticancer effects of a REIC/Dkk-3-encoding adenoviral vector

2013 ◽  
Vol 31 (3) ◽  
pp. 1089-1095 ◽  
Author(s):  
MASAMI WATANABE ◽  
MASAKIYO SAKAGUCHI ◽  
RIE KINOSHITA ◽  
HARUKI KAKU ◽  
YUICHI ARIYOSHI ◽  
...  
Keyword(s):  
Gene Expression ◽  
Adenoviral Vector ◽  
Expression System ◽  
Gene Expression System ◽  
Novel Gene ◽  
Anticancer Effects

scholarly journals A novel gene expression system for Ralstonia eutropha based on the T7 promoter

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Muzi Hu ◽  
Bin Xiong ◽  
Zhongkang Li ◽  
Li Liu ◽  
Siwei Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ralstonia Eutropha ◽  
Expression System ◽  
T7 Promoter ◽  
Gene Expression System ◽  
Novel Gene

scholarly journals Harnessing a Transient Gene Expression System in Nicotiana benthamiana to Explore Plant Agrochemical Transporters

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 524
Author(s):  
Bingqi Wu ◽  
Zhiting Chen ◽  
Xiaohui Xu ◽  
Ronghua Chen ◽  
Siwei Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Functional Characterization ◽  
Transient Gene Expression ◽  
High Mobility ◽  
Gene Expression System

Functional characterization of plant agrichemical transporters provided an opportunity to discover molecules that have a high mobility in plants and have the potential to increase the amount of pesticides reaching damage sites. Agrobacterium-mediated transient expression in tobacco is simple and fast, and its protein expression efficiency is high; this system is generally used to mediate heterologous gene expression. In this article, transient expression of tobacco nicotine uptake permease (NtNUP1) and rice polyamine uptake transporter 1 (OsPUT1) in Nicotiana benthamiana was performed to investigate whether this system is useful as a platform for studying the interactions between plant transporters and pesticides. The results showed that NtNUP1 increases nicotine uptake in N. benthamiana foliar discs and protoplasts, indicating that this transient gene expression system is feasible for studying gene function. Moreover, yeast expression of OsPUT1 apparently increases methomyl uptake. Overall, this method of constructing a transient gene expression system is useful for improving the efficiency of analyzing the functions of plant heterologous transporter-encoding genes and revealed that this system can be further used to study the functions of transporters and pesticides, especially their interactions.

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