scholarly journals Kinetic analysis of a large facilitatory action of 4-aminopyridine on the motor nerve terminal of the neuromuscular junction.

1980 ◽  
Vol 56 (4) ◽  
pp. 241-245 ◽  
Author(s):  
Takashi MAENO
Keyword(s):  
Neuromuscular Junction ◽  
Kinetic Analysis ◽  
Nerve Terminal ◽  
Motor Nerve ◽  
Motor Nerve Terminal ◽  
Facilitatory Action

scholarly journals Interaction of 125I-labeled botulinum neurotoxins with nerve terminals. I. Ultrastructural autoradiographic localization and quantitation of distinct membrane acceptors for types A and B on motor nerves.

1986 ◽  
Vol 103 (2) ◽  
pp. 521-534 ◽  
Author(s):  
J D Black ◽  
J O Dolly
Keyword(s):  
Plasma Membrane ◽  
Neuromuscular Junction ◽  
Nerve Terminal ◽  
Motor Nerve ◽  
Type A ◽  
Nerve Trunk ◽  
Metabolic Inhibitors ◽  
Motor Nerve Terminal ◽  
Electron Microscopic Autoradiography

The labeling patterns produced by radioiodinated botulinum neurotoxin (125I-BoNT) types A and B at the vertebrate neuromuscular junction were investigated using electron microscopic autoradiography. The data obtained allow the following conclusions to be made. 125I-BoNT type A, applied in vivo or in vitro to mouse diaphragm or frog cutaneous pectoris muscle, interacts saturably with the motor nerve terminal only; silver grains occur on the plasma membrane, within the synaptic bouton, and in the axoplasm of the nerve trunk, suggesting internalization and retrograde intra-axonal transport of toxin or fragments thereof. 125I-BoNT type B, applied in vitro to the murine neuromuscular junction, interacts likewise with the motor nerve terminal except that a lower proportion of internalized radioactivity is seen. This result is reconcilable with the similar, but not identical, pharmacological action of these toxin types. The saturability of labeling in each case suggested the involvement of acceptors; on preventing the internalization step with metabolic inhibitors, their precise location became apparent. They were found on all unmyelinated areas of the nerve terminal membrane, including the preterminal axon and the synaptic bouton. Although 125I-BoNT type A interacts specifically with developing terminals of newborn rats, the unmyelinated plasma membrane of the nerve trunk is not labeled, indicating that the acceptors are unique components restricted to the nerve terminal area. BoNT types A and B have distinct acceptors on the terminal membrane. Having optimized the conditions for saturation of these binding sites and calibrated the autoradiographic procedure, we found the densities of the acceptors for types A and B to be approximately 150 and 630/micron 2 of membrane, respectively. It is proposed that these membrane acceptors target BoNT to the nerve terminal and mediate its delivery to an intracellular site, thus contributing to the toxin's selective inhibitory action on neurotransmitter release.

Steroid anaesthetics: inhibition of depolarization–secretion coupling at the mouse motor nerve terminal

1980 ◽  
Vol 58 (10) ◽  
pp. 1221-1228 ◽  
Author(s):  
P. Pennefather ◽  
E. Puil ◽  
D. M. J. Quastel
Keyword(s):  
Neuromuscular Junction ◽  
Nerve Terminal ◽  
Motor Nerve ◽  
Motor Nerve Terminal ◽  
Nerve Terminals ◽  
Potassium Depolarization ◽  
End Plate

The coupling between nerve terminal depolarization and quantal secretion of acetylcholine at the mouse neuromuscular junction was estimated by measuring the multiplication of the frequency of miniature end-plate potentials (m.e.p.p.s) produced by increasing the concentration of calcium in the medium from 0.1 to 1.0 mM in the presence of 15 mM potassium. Depolarization–secretion coupling was inhibited by the anaesthetic steroids progesterone, pregnanedione, and alphaxalone. The nonanaesthetic steroid Δ16-alphaxalone also inhibited depolarization–secretion coupling with the same potency as alphaxalone. This result indicates that inhibition of depolarization–secretion coupling in nerve terminals is unlikely to play a major role in the production of anaesthesia.

Motor nerve terminal loss from degenerating muscle fibers

Neuron ◽  
1989 ◽  
Vol 3 (6) ◽  
pp. 677-688 ◽  
Author(s):  
Mark Rich ◽  
Jeff W. Lichtman
Keyword(s):  
Nerve Terminal ◽  
Motor Nerve ◽  
Muscle Fibers ◽  
Motor Nerve Terminal

Motor nerve terminal morphologic plasticity induced by small changes in the locomotor activtty of the adult rat

1990 ◽  
Vol 14 ◽  
pp. 227
Author(s):  
J TOMAS ◽  
R FENOLL ◽  
J BATLLE ◽  
M SANTAFE ◽  
V PIERA ◽  
...  
Keyword(s):  
Nerve Terminal ◽  
Motor Nerve ◽  
Motor Nerve Terminal ◽  
Adult Rat

Bursts of miniature end-plate potentials can be released from localized regions of the frog motor nerve terminal

1981 ◽  
Vol 221 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Ira S. Cohen ◽  
William Van Der Kloot ◽  
Stuart B. Barton
Keyword(s):  
Nerve Terminal ◽  
Motor Nerve ◽  
Motor Nerve Terminal ◽  
End Plate
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