Study of Linseed Protein Extraction and Purification : Impact of Process Conditions on Protein Functional and Structural Properties

2021 ◽  
Author(s):  
Sara Albe Slabi ◽  
Sophie Beaubier ◽  
Mbalo Ndiaye ◽  
Olivier Galet ◽  
Romain Kapel
Keyword(s):  
Structural Properties ◽  
Protein Extraction ◽  
Process Conditions ◽  
Extraction And Purification

scholarly journals The Freeze-Drying of Foods—The Characteristic of the Process Course and the Effect of Its Parameters on the Physical Properties of Food Materials

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1488 ◽  
Author(s):  
Dorota Nowak ◽  
Ewa Jakubczyk
Keyword(s):  
Mechanical Properties ◽  
Physical Properties ◽  
Structural Properties ◽  
Freeze Drying ◽  
Process Conditions ◽  
Drying Process ◽  
High Quality ◽  
Freeze Dried ◽  
Secondary Drying ◽  
Selection Of

Freeze-drying, also known as lyophilization, is a process in which water in the form of ice under low pressure is removed from a material by sublimation. This process has found many applications for the production of high quality food and pharmaceuticals. The main steps of the freeze-drying process, such as the freezing of the product and primary and secondary drying, are described in this paper. The problems and mechanisms of each step of the freeze-drying process are also analyzed. The methods necessary for the selection of the primary and secondary end processes are characterized. The review contains a description of the effects of process conditions and the selected physical properties of freeze-dried materials, such as structural properties (shrinkage and density porosity), color, and texture. The study shows that little attention is given to the mechanical properties and texture of freeze-dried materials obtained from different conditions of the lyophilization process.

Sweet potato.

2021 ◽  
pp. 61-84
Author(s):  
S. N. Moorthy ◽  
M. S. Sajeev ◽  
R. P. K. Ambrose ◽  
R. J. Anish
Keyword(s):  
Thermal Properties ◽  
Rheological Property ◽  
Structural Properties ◽  
Sweet Potatoes ◽  
Physiochemical Properties ◽  
Biochemical Characteristics ◽  
Extraction And Purification ◽  
Granule Morphology ◽  
Starch Crystallinity

Abstract This book chapter outlines the extraction and purification, physiochemical properties (i.e. biochemical characteristics, amylose and amylopectin content), structural properties (i.e. granule morphology, XRD and starch crystallinity, structure of amylose and amylopectin), functional properties (i.e. swelling pattern and solubility, viscosity, rheological property, retrogradation), thermal properties (i.e. DSC), and digestibility of sweet potatoes.

scholarly journals Membrane protein extraction and purification using partially-esterified SMA polymers

2021 ◽  
pp. 183758
Author(s):  
Olivia P. Hawkins ◽  
Christine Parisa T. Jahromi ◽  
Aiman A. Gulamhussein ◽  
Stephanie Nestorow ◽  
Taranpreet Bahra ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Extraction ◽  
Extraction And Purification

scholarly journals DNA, RNA, and Protein Extraction: The Past and The Present

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Siun Chee Tan ◽  
Beow Chin Yiap
Keyword(s):  
Mechanical Treatment ◽  
Protein Extraction ◽  
Cross Contamination ◽  
Automated Systems ◽  
The Past ◽  
Extraction And Purification ◽  
Manual Methods ◽  
Downstream Processes ◽  
Speed Accuracy ◽  
Over Time

Extraction of DNA, RNA, and protein is the basic method used in molecular biology. These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes. In the past, the process of extraction and purification of nucleic acids used to be complicated, time-consuming, labor-intensive, and limited in terms of overall throughput. Currently, there are many specialized methods that can be used to extract pure biomolecules, such as solution-based and column-based protocols. Manual method has certainly come a long way over time with various commercial offerings which included complete kits containing most of the components needed to isolate nucleic acid, but most of them require repeated centrifugation steps, followed by removal of supernatants depending on the type of specimen and additional mechanical treatment. Automated systems designed for medium-to-large laboratories have grown in demand over recent years. It is an alternative to labor-intensive manual methods. The technology should allow a high throughput of samples; the yield, purity, reproducibility, and scalability of the biomolecules as well as the speed, accuracy, and reliability of the assay should be maximal, while minimizing the risk of cross-contamination.

scholarly journals Molecular farming on rescue of pharma industry for next generations

2018 ◽  
Author(s):  
Khaled Moustafa ◽  
Jocelyne Trémouillaux-Guiller
Keyword(s):  
Recombinant Proteins ◽  
Protein Extraction ◽  
Molecular Farming ◽  
Proper Function ◽  
Protein Accumulation ◽  
Post Translational Modifications ◽  
Extraction And Purification ◽  
Animal Pathogens ◽  
Transformation Methods ◽  
Traditional Approaches

Recombinant proteins expressed in plants have been emerged as a novel branch of the biopharmaceutical industry, offering practical and safety advantages over traditional approaches. Cultivable in various platforms (i.e. open field, greenhouses or bioreactors), plants hold great potential to produce different types of therapeutic proteins with reduced risks of contamination with human and animal pathogens. To maximize the yield and quality of plant-made pharmaceuticals, crucial factors should be taken into account, including host plants, expression cassettes, subcellular localization, post-translational modifications, and protein extraction and purification methods. DNA technology and genetic transformation methods have also contributed to great parts with substantial improvements. To play their proper function and stability, proteins require multiple post-translational modifications such as glycosylation. Intensive glycoengineering research has been performed to reduce the immunogenicity of recombinant proteins produced in plants. Important strategies have also been developed to minimize the proteolysis effects and enhance protein accumulation. With growing human population and new epidemic threats, the need for new medications will be paramount so that the traditional pharmaceutical industry will not be alone to answer medication demands for upcoming generations. Here, we review several aspects of plant molecular pharming and outline some important challenges that hamper these ambitious biotechnological developments.

Influence of process conditions on pretreatment of microalgae for protein extraction and production of biocrude during hydrothermal liquefaction of pretreated Tetraselmis sp.

RSC Advances ◽  
2015 ◽  
Vol 5 (26) ◽  
pp. 20193-20207 ◽  
Author(s):  
B. E. Eboibi ◽  
D. M. Lewis ◽  
P. J. Ashman ◽  
S. Chinnasamy
Keyword(s):  
Protein Extraction ◽  
Direct Conversion ◽  
Hydrothermal Liquefaction ◽  
Process Conditions ◽  
Advanced Biofuels ◽  
Attractive Option

The direct conversion of microalgae to advanced biofuels with hydrothermal liquefaction (HTL) is an attractive option which has drawn attention in recent years.

Aroids.

2021 ◽  
pp. 85-114
Author(s):  
S. N. Moorthy ◽  
M. S. Sajeev ◽  
R. P. K. Ambrose ◽  
R. J. Anish
Keyword(s):  
Thermal Properties ◽  
Rheological Properties ◽  
Structural Properties ◽  
Functional Properties ◽  
Extraction And Purification ◽  
Granular Morphology ◽  
Amylopectin Structure ◽  
Starch Crystallinity

Abstract This book chapter outlines the extraction and purification, physiochemical composition (i.e. biochemical content, amylose and amylopectin content), structural properties (i.e. granular morphology, XRD and starch crystallinity, amylose and amylopectin structure), functional properties (i.e. swelling and solubility, viscosity, rheological properties, retrogradation), thermal properties (i.e. DSC, digestibility) of aroids.

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