Aptamer-Based Affinity Chromatography for Protein Extraction and Purification

2019 ◽  
pp. 93-139 ◽  
Author(s):  
G. Perret ◽  
E. Boschetti
Keyword(s):  
Affinity Chromatography ◽  
Protein Extraction ◽  
Extraction And Purification

scholarly journals Membrane protein extraction and purification using partially-esterified SMA polymers

2021 ◽  
pp. 183758
Author(s):  
Olivia P. Hawkins ◽  
Christine Parisa T. Jahromi ◽  
Aiman A. Gulamhussein ◽  
Stephanie Nestorow ◽  
Taranpreet Bahra ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Extraction ◽  
Extraction And Purification

scholarly journals DNA, RNA, and Protein Extraction: The Past and The Present

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
Siun Chee Tan ◽  
Beow Chin Yiap
Keyword(s):  
Mechanical Treatment ◽  
Protein Extraction ◽  
Cross Contamination ◽  
Automated Systems ◽  
The Past ◽  
Extraction And Purification ◽  
Manual Methods ◽  
Downstream Processes ◽  
Speed Accuracy ◽  
Over Time

Extraction of DNA, RNA, and protein is the basic method used in molecular biology. These biomolecules can be isolated from any biological material for subsequent downstream processes, analytical, or preparative purposes. In the past, the process of extraction and purification of nucleic acids used to be complicated, time-consuming, labor-intensive, and limited in terms of overall throughput. Currently, there are many specialized methods that can be used to extract pure biomolecules, such as solution-based and column-based protocols. Manual method has certainly come a long way over time with various commercial offerings which included complete kits containing most of the components needed to isolate nucleic acid, but most of them require repeated centrifugation steps, followed by removal of supernatants depending on the type of specimen and additional mechanical treatment. Automated systems designed for medium-to-large laboratories have grown in demand over recent years. It is an alternative to labor-intensive manual methods. The technology should allow a high throughput of samples; the yield, purity, reproducibility, and scalability of the biomolecules as well as the speed, accuracy, and reliability of the assay should be maximal, while minimizing the risk of cross-contamination.

scholarly journals Molecular farming on rescue of pharma industry for next generations

2018 ◽  
Author(s):  
Khaled Moustafa ◽  
Jocelyne Trémouillaux-Guiller
Keyword(s):  
Recombinant Proteins ◽  
Protein Extraction ◽  
Molecular Farming ◽  
Proper Function ◽  
Protein Accumulation ◽  
Post Translational Modifications ◽  
Extraction And Purification ◽  
Animal Pathogens ◽  
Transformation Methods ◽  
Traditional Approaches

Recombinant proteins expressed in plants have been emerged as a novel branch of the biopharmaceutical industry, offering practical and safety advantages over traditional approaches. Cultivable in various platforms (i.e. open field, greenhouses or bioreactors), plants hold great potential to produce different types of therapeutic proteins with reduced risks of contamination with human and animal pathogens. To maximize the yield and quality of plant-made pharmaceuticals, crucial factors should be taken into account, including host plants, expression cassettes, subcellular localization, post-translational modifications, and protein extraction and purification methods. DNA technology and genetic transformation methods have also contributed to great parts with substantial improvements. To play their proper function and stability, proteins require multiple post-translational modifications such as glycosylation. Intensive glycoengineering research has been performed to reduce the immunogenicity of recombinant proteins produced in plants. Important strategies have also been developed to minimize the proteolysis effects and enhance protein accumulation. With growing human population and new epidemic threats, the need for new medications will be paramount so that the traditional pharmaceutical industry will not be alone to answer medication demands for upcoming generations. Here, we review several aspects of plant molecular pharming and outline some important challenges that hamper these ambitious biotechnological developments.

scholarly journals Seaweed Protein Hydrolysates and Bioactive Peptides: Extraction, Purification and Applications

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 500
Author(s):  
Javier Echave ◽  
Maria Fraga-Corral ◽  
Pascual Garcia-Perez ◽  
Jelena Popović-Djordjević ◽  
Edina H. Avdović ◽  
...  
Keyword(s):  
Cell Wall ◽  
Protein Extraction ◽  
Sample Pretreatment ◽  
Industrial Applications ◽  
Extraction Yield ◽  
Protein Hydrolysates ◽  
Extraction And Purification ◽  
Assisted Extraction ◽  
Pharmaceutical Industries ◽  
Solid Liquid

Seaweeds are industrially exploited for obtaining pigments, polysaccharides, or phenolic compounds with application in diverse fields. Nevertheless, their rich composition in fiber, minerals, and proteins, has pointed them as a useful source of these components. Seaweed proteins are nutritionally valuable and include several specific enzymes, glycoproteins, cell wall-attached proteins, phycobiliproteins, lectins, or peptides. Extraction of seaweed proteins requires the application of disruptive methods due to the heterogeneous cell wall composition of each macroalgae group. Hence, non-protein molecules like phenolics or polysaccharides may also be co-extracted, affecting the extraction yield. Therefore, depending on the macroalgae and target protein characteristics, the sample pretreatment, extraction and purification techniques must be carefully chosen. Traditional methods like solid–liquid or enzyme-assisted extraction (SLE or EAE) have proven successful. However, alternative techniques as ultrasound- or microwave-assisted extraction (UAE or MAE) can be more efficient. To obtain protein hydrolysates, these proteins are subjected to hydrolyzation reactions, whether with proteases or physical or chemical treatments that disrupt the proteins native folding. These hydrolysates and derived peptides are accounted for bioactive properties, like antioxidant, anti-inflammatory, antimicrobial, or antihypertensive activities, which can be applied to different sectors. In this work, current methods and challenges for protein extraction and purification from seaweeds are addressed, focusing on their potential industrial applications in the food, cosmetic, and pharmaceutical industries.

Overview on Protein Extraction and Purification Using Ionic-Liquid-Based Processes

2021 ◽  
Author(s):  
João C. F. Nunes ◽  
Mafalda R. Almeida ◽  
Joaquim L. Faria ◽  
Cláudia G. Silva ◽  
Márcia C. Neves ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Protein Extraction ◽  
Extraction And Purification

Artificial stabilization of the fusion pore by intra-organelle styrene-maleic acid copolymers

Soft Matter ◽  
2021 ◽  
Author(s):  
Marcelo Caparotta ◽  
Marcelo Puiatti ◽  
Diego Masone
Keyword(s):  
Membrane Protein ◽  
Maleic Acid ◽  
Protein Extraction ◽  
Fusion Pore ◽  
Protein Isolation ◽  
Free Alternative ◽  
Extraction And Purification ◽  
Lipid Environment

Styrene-maleic acid copolymers have become an advantageous detergent-free alternative for membrane protein isolation. Since their discovery, experimental membrane protein extraction and purification by keeping intact their lipid environment has become...

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