scholarly journals Function and dynamics of aptamers: A case study on the malachite green aptamer

2008 ◽  
Author(s):  
Tianjiao Wang
Keyword(s):  
Malachite Green ◽  
Malachite Green Aptamer

scholarly journals Thermodynamics of Ligand Binding to a Heterogeneous RNA Population in the Malachite Green Aptamer

Biochemistry ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 565-572 ◽  
Author(s):  
Joshua E. Sokoloski ◽  
Sarah E. Dombrowski ◽  
Philip C. Bevilacqua
Keyword(s):  
Ligand Binding ◽  
Malachite Green ◽  
Malachite Green Aptamer

2.8 Å crystal structure of the malachite green aptamer 1 1Edited by J. A. Doudna

2000 ◽  
Vol 301 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Christopher Baugh ◽  
Dilârâ Grate ◽  
Charles Wilson
Keyword(s):  
Crystal Structure ◽  
Malachite Green ◽  
Malachite Green Aptamer

scholarly journals Labeling RNAs in live cells using malachite green aptamer scaffolds as fluorescent probes

2017 ◽  
Author(s):  
V. Siddartha Yerramilli ◽  
Kyung Hyuk Kim
Keyword(s):  
Malachite Green ◽  
Tandem Repeats ◽  
Fluorescent Proteins ◽  
Rna Aptamers ◽  
Live Cells ◽  
High Background ◽  
Rna Molecules ◽  
High Background Noise ◽  
Fluorescent Tags ◽  
Malachite Green Aptamer

AbstractRNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamerfluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA), an RNA aptamer that can specifically bind to malachite green (MG) dye and induces it to emit far-red fluorescence signals. Previous studies on MGA showed a potential for the use of MGA for genetically tagging other RNA molecules in live cells. However, these studies also exhibited low fluorescence signals and high background noise. Here we constructed and tested RNA scaffolds containing multiple tandem repeats of MGA as a strategy to increase the brightness of the MGA aptamer-fluorogen system as well as to make the system fluoresce when tagging various RNA molecules, in live cells. We demonstrate that our MGA scaffolds can induce fluorescence signals by up to ~20 fold compared to the basal level as a genetic tag for other RNA molecules. We also show that our scaffolds function reliably as genetically-encoded fluorescent tags for mRNAs of fluorescent proteins and other RNA aptamers.

The Genesis and Internal Dynamics of El Salvador's People's Revolutionary Army, 1970–1976

2014 ◽  
Vol 38 (01) ◽  
pp. 102-129
Author(s):  
ALBERTO MARTÍN ÁLVAREZ ◽  
EUDALD CORTINA ORERO
Keyword(s):  
Latin America ◽  
El Salvador ◽  
Early Years ◽  
Left Wing ◽  
Internal Dynamics ◽  
Internal Coherence ◽  
The 1960S

AbstractUsing interviews with former militants and previously unpublished documents, this article traces the genesis and internal dynamics of the Ejército Revolucionario del Pueblo (People's Revolutionary Army, ERP) in El Salvador during the early years of its existence (1970–6). This period was marked by the inability of the ERP to maintain internal coherence or any consensus on revolutionary strategy, which led to a series of splits and internal fights over control of the organisation. The evidence marshalled in this case study sheds new light on the origins of the armed Salvadorean Left and thus contributes to a wider understanding of the processes of formation and internal dynamics of armed left-wing groups that emerged from the 1960s onwards in Latin America.

Culture and the plasticity of perception

2020 ◽  
Vol 43 ◽  
Author(s):  
Michael Lifshitz ◽  
T. M. Luhrmann
Keyword(s):  
Cultural Context ◽  
Sensory Experience ◽  
Cultural Learning ◽  
The World

Abstract Culture shapes our basic sensory experience of the world. This is particularly striking in the study of religion and psychosis, where we and others have shown that cultural context determines both the structure and content of hallucination-like events. The cultural shaping of hallucinations may provide a rich case-study for linking cultural learning with emerging prediction-based models of perception.

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