Binary Malachite Green Aptamer for Fluorescent Detection of Nucleic Acids

2005 ◽  
Vol 127 (36) ◽  
pp. 12442-12443 ◽  
Author(s):  
Dmitry M. Kolpashchikov
Keyword(s):  
Nucleic Acids ◽  
Malachite Green ◽  
Fluorescent Detection ◽  
Malachite Green Aptamer

scholarly journals An LED-Driven AuNPs-PDMS Microfluidic Chip and Integrated Device for the Detection of Digital Loop-Mediated Isothermal DNA Amplification

Micromachines ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 177 ◽  
Author(s):  
Zengming Zhang ◽  
Shuhao Zhao ◽  
Fei Hu ◽  
Guangpu Yang ◽  
Juan Li ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Microfluidic Chip ◽  
Cross Flow ◽  
Flow Channel ◽  
Isothermal Amplification ◽  
Fluorescent Detection ◽  
Lamp Method ◽  
Biological Studies ◽  
Micro Cavity ◽  
Integrated Device

The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption.

scholarly journals Thermodynamics of Ligand Binding to a Heterogeneous RNA Population in the Malachite Green Aptamer

Biochemistry ◽  
2011 ◽  
Vol 51 (1) ◽  
pp. 565-572 ◽  
Author(s):  
Joshua E. Sokoloski ◽  
Sarah E. Dombrowski ◽  
Philip C. Bevilacqua
Keyword(s):  
Ligand Binding ◽  
Malachite Green ◽  
Malachite Green Aptamer

2.8 Å crystal structure of the malachite green aptamer 1 1Edited by J. A. Doudna

2000 ◽  
Vol 301 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Christopher Baugh ◽  
Dilârâ Grate ◽  
Charles Wilson
Keyword(s):  
Crystal Structure ◽  
Malachite Green ◽  
Malachite Green Aptamer

Multicolor instrumentation for direct fluorescent detection of nucleic acids in a microchip format

1998 ◽  
Author(s):  
Valery L. Bogdanov ◽  
Yu-Hui Rogers ◽  
Guang Lan ◽  
Michael Boyce-Jacino
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Detection

scholarly journals Interaction of cyanine dyes with nucleic acids. Meso-substituted trimethincyanines for fluorescent detection of nucleic acids

2002 ◽  
Vol 18 (3) ◽  
pp. 243-252 ◽  
Author(s):  
S. S. Lukashov ◽  
M. Yu. Losytskyy ◽  
Yu. P. Kovtun ◽  
S. M. Yarmoluk
Keyword(s):  
Nucleic Acids ◽  
Cyanine Dyes ◽  
Fluorescent Detection

scholarly journals Symmetric exponential amplification reaction-based DNA nanomachine for the fluorescent detection of nucleic acids

RSC Advances ◽  
2019 ◽  
Vol 9 (70) ◽  
pp. 41305-41310
Author(s):  
Qi Yan ◽  
Qiuyue Duan ◽  
Yuqi Huang ◽  
Jing Guo ◽  
Liang Zhong ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Fluorescent Detection ◽  
Label Free ◽  
Background Signal ◽  
Low Background ◽  
Amplification Reaction ◽  
Dna Nanomachine

This label-free and ultra-low background signal DNA nanomachine was based on P-HP mediated S-EXPAR and the G-triplex/ThT complex.

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