scholarly journals Field-Scale Evaluation of Biological Uranium Reduction and Reoxidation in the Near-Source Zone at the NABIR Field Research Center in Oak Ridge, TN

2006 ◽  
Author(s):  
Craig S. Criddle
Keyword(s):  
Field Research ◽  
Source Zone ◽  
Research Center ◽  
Field Scale ◽  
Uranium Reduction ◽  
Scale Evaluation ◽  
Oak Ridge

scholarly journals Field-scale evaluation of biological uranium reduction and reoxidation in the near-source zone at the NABIR Field Research Center in Oak Ridge, TN

2006 ◽  
Author(s):  
Craig S. Criddle ◽  
Peter Kitanidis ◽  
Scott Fendorf ◽  
Weimin Wu ◽  
Philip M. Jardine ◽  
...  
Keyword(s):  
Field Research ◽  
Source Zone ◽  
Research Center ◽  
Field Scale ◽  
Uranium Reduction ◽  
Scale Evaluation ◽  
Oak Ridge

scholarly journals Draft Genome Sequences of Two Janthinobacterium lividum Strains, Isolated from Pristine Groundwater Collected from the Oak Ridge Field Research Center

2017 ◽  
Vol 5 (26) ◽  
Author(s):  
Xiaoqin Wu ◽  
Adam M. Deutschbauer ◽  
Alexey E. Kazakov ◽  
Kelly M. Wetmore ◽  
Bryson A. Cwick ◽  
...  
Keyword(s):  
Draft Genome ◽  
Field Research ◽  
Research Center ◽  
Purple Pigment ◽  
Genome Sequences ◽  
Content Type ◽  
Oak Ridge ◽  
Background Site ◽  
Janthinobacterium Lividum ◽  
Groundwater Samples

ABSTRACT We present here the draft genome sequences of two Janthinobacterium lividum strains, GW456P and GW458P, isolated from groundwater samples collected from a background site at the Oak Ridge Field Research Center. Production of a purple pigment by these two strains was observed when grown on diluted (1/10) LB agar plates.

scholarly journals Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC), Oak Ridge Tennessee

2006 ◽  
Author(s):  
David Watson ◽  
Philip Jardine ◽  
Baohua Gu ◽  
Jack Parker ◽  
Craig Brandt ◽  
...  
Keyword(s):  
Field Research ◽  
Research Center ◽  
Oak Ridge

scholarly journals Complete Genome Sequence of Cupriavidus basilensis 4G11, Isolated from the Oak Ridge Field Research Center Site

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Jayashree Ray ◽  
R. Jordan Waters ◽  
Jeffrey M. Skerker ◽  
Jennifer V. Kuehl ◽  
Morgan N. Price ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Field Research ◽  
Research Center ◽  
Oak Ridge ◽  
Cupriavidus Basilensis ◽  
Center Site

scholarly journals Denitrifying Bacteria Isolated from Terrestrial Subsurface Sediments Exposed to Mixed-Waste Contamination

2010 ◽  
Vol 76 (10) ◽  
pp. 3244-3254 ◽  
Author(s):  
Stefan J. Green ◽  
Om Prakash ◽  
Thomas M. Gihring ◽  
Denise M. Akob ◽  
Puja Jasrotia ◽  
...  
Keyword(s):  
Nitrous Oxide ◽  
Field Research ◽  
Denitrifying Bacteria ◽  
Nitrate Contamination ◽  
Source Zone ◽  
Metagenomic Data ◽  
Rrna Gene ◽  
Oak Ridge ◽  
Subsurface Sediments ◽  
Terrestrial Subsurface

ABSTRACTIn terrestrial subsurface environments where nitrate is a critical groundwater contaminant, few cultivated representatives are available to verify the metabolism of organisms that catalyze denitrification. In this study, five species of denitrifying bacteria from three phyla were isolated from subsurface sediments exposed to metal radionuclide and nitrate contamination as part of the U.S. Department of Energy's Oak Ridge Integrated Field Research Challenge (OR-IFRC). Isolates belonged to the generaAfipiaandHyphomicrobium(Alphaproteobacteria),Rhodanobacter(Gammaproteobacteria),Intrasporangium(Actinobacteria), andBacillus(Firmicutes). Isolates from the phylumProteobacteriawere complete denitrifiers, whereas the Gram-positive isolates reduced nitrate to nitrous oxide. rRNA gene analyses coupled with physiological and genomic analyses suggest that bacteria from the genusRhodanobacterare a diverse population of denitrifiers that are circumneutral to moderately acidophilic, with a high relative abundance in areas of the acidic source zone at the OR-IFRC site. Based on genome analysis,Rhodanobacterspecies contain two nitrite reductase genes and have not been detected in functional-gene surveys of denitrifying bacteria at the OR-IFRC site. Nitrite and nitrous oxide reductase gene sequences were recovered from the isolates and from the terrestrial subsurface by designing primer sets mined from genomic and metagenomic data and from draft genomes of two of the isolates. We demonstrate that a combination of cultivation and genomic and metagenomic data is essential to thein situcharacterization of denitrifiers and that current PCR-based approaches are not suitable for deep coverage of denitrifiers. Our results indicate that the diversity of denitrifiers is significantly underestimated in the terrestrial subsurface.

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