scholarly journals Quality Assurance Plan for Field Activities at the Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC), Oak Ridge, Tennessee

2002 ◽  
Author(s):  
C C Brandt
Keyword(s):  
Quality Assurance ◽  
Field Research ◽  
Research Center ◽  
Oak Ridge

scholarly journals Draft Genome Sequences of Two Janthinobacterium lividum Strains, Isolated from Pristine Groundwater Collected from the Oak Ridge Field Research Center

2017 ◽  
Vol 5 (26) ◽  
Author(s):  
Xiaoqin Wu ◽  
Adam M. Deutschbauer ◽  
Alexey E. Kazakov ◽  
Kelly M. Wetmore ◽  
Bryson A. Cwick ◽  
...  
Keyword(s):  
Draft Genome ◽  
Field Research ◽  
Research Center ◽  
Purple Pigment ◽  
Genome Sequences ◽  
Content Type ◽  
Oak Ridge ◽  
Background Site ◽  
Janthinobacterium Lividum ◽  
Groundwater Samples

ABSTRACT We present here the draft genome sequences of two Janthinobacterium lividum strains, GW456P and GW458P, isolated from groundwater samples collected from a background site at the Oak Ridge Field Research Center. Production of a purple pigment by these two strains was observed when grown on diluted (1/10) LB agar plates.

scholarly journals Field-scale evaluation of biological uranium reduction and reoxidation in the near-source zone at the NABIR Field Research Center in Oak Ridge, TN

2006 ◽  
Author(s):  
Craig S. Criddle ◽  
Peter Kitanidis ◽  
Scott Fendorf ◽  
Weimin Wu ◽  
Philip M. Jardine ◽  
...  
Keyword(s):  
Field Research ◽  
Source Zone ◽  
Research Center ◽  
Field Scale ◽  
Uranium Reduction ◽  
Scale Evaluation ◽  
Oak Ridge

scholarly journals Natural and Accelerated Bioremediation Research (NABIR) Field Research Center (FRC), Oak Ridge Tennessee

2006 ◽  
Author(s):  
David Watson ◽  
Philip Jardine ◽  
Baohua Gu ◽  
Jack Parker ◽  
Craig Brandt ◽  
...  
Keyword(s):  
Field Research ◽  
Research Center ◽  
Oak Ridge

scholarly journals Complete Genome Sequence of Cupriavidus basilensis 4G11, Isolated from the Oak Ridge Field Research Center Site

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Jayashree Ray ◽  
R. Jordan Waters ◽  
Jeffrey M. Skerker ◽  
Jennifer V. Kuehl ◽  
Morgan N. Price ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Field Research ◽  
Research Center ◽  
Oak Ridge ◽  
Cupriavidus Basilensis ◽  
Center Site

Hydrogeophysical investigations of the former S-3 ponds contaminant plumes, Oak Ridge Integrated Field Research Challenge site, Tennessee

Geophysics ◽  
2013 ◽  
Vol 78 (4) ◽  
pp. EN29-EN41 ◽  
Author(s):  
A. Revil ◽  
M. Skold ◽  
M. Karaoulis ◽  
M. Schmutz ◽  
S. S. Hubbard ◽  
...  
Keyword(s):  
Field Research ◽  
Clay Content ◽  
Surface Conductivity ◽  
Dc Resistivity ◽  
High Concentration ◽  
Resistivity Tomography ◽  
Contaminant Plumes ◽  
Oak Ridge ◽  
Nitrate Concentrations ◽  
Research Challenge

At the Oak Ridge Integrated Field Research Challenge site, near Oak Ridge, Tennessee, contaminants from the former S-3 ponds have infiltrated the shallow saprolite for over 60 years. Two- and three-dimensional DC-resistivity tomography is used to characterize the number and location of the main contaminant plumes, which include high concentration of nitrate. These contaminant plumes have typically an electrical resistivity in the range 2–20 ohm-m while the background saprolite resistivity is in the range 60–120 ohm-m, so the difference of resistivity can be easily mapped using DC-resistivity tomography to locate the contaminant pathways. We develop a relationship to derive the in situ nitrate concentrations from the 3D resistivity tomograms accounting for the effect of surface conductivity. The footprint of the contamination upon the resistivity is found to be much stronger than the local variations associated with changes in the porosity and the clay content. With this method, we identified a total of five main plumes (termed CP1 to CP5). Plume CP2 corresponds to the main plume in terms of nitrate concentration (∼50,000 [Formula: see text]). We also used an active time constrained approach to perform time-lapse resistivity tomography over a section crossing the plumes CP1 and CP2. The sequence of tomograms is used to determine the changes in the nitrate concentrations associated with infiltration of fresh (meteoritic) water from a perched aquifer. This study highlights the importance of accounting for surface conductivity when characterizing plume distributions in clay-rich subsurface systems.

scholarly journals Isolation, Characterization, and U(VI)-Reducing Potential of a Facultatively Anaerobic, Acid-Resistant Bacterium from Low-pH, Nitrate- and U(VI)-Contaminated Subsurface Sediment and Description of Salmonella subterranea sp. nov

2004 ◽  
Vol 70 (5) ◽  
pp. 2959-2965 ◽  
Author(s):  
Evgenya S. Shelobolina ◽  
Sara A. Sullivan ◽  
Kathleen R. O'Neill ◽  
Kelly P. Nevin ◽  
Derek R. Lovley
Keyword(s):  
Enrichment Culture ◽  
Phylogenetic Analyses ◽  
Field Research ◽  
Low Ph ◽  
Research Field ◽  
Resistant Bacterium ◽  
Subsurface Sediment ◽  
Oak Ridge ◽  
Facultatively Anaerobic ◽  
Rdna Sequences

ABSTRACT A facultatively anaerobic, acid-resistant bacterium, designated strain FRCl, was isolated from a low-pH, nitrate- and U(VI)-contaminated subsurface sediment at site FW-024 at the Natural and Accelerated Bioremediation Research Field Research Center in Oak Ridge, Tenn. Strain FRCl was enriched at pH 4.5 in minimal medium with nitrate as the electron acceptor, hydrogen as the electron donor, and acetate as the carbon source. Clones with 16S ribosomal DNA (rDNA) sequences identical to the sequence of strain FRCl were also detected in a U(VI)-reducing enrichment culture derived from the same sediment. Cells of strain FRCl were gram-negative motile regular rods 2.0 to 3.4 μm long and 0.7 to 0.9 μm in diameter. Strain FRCl was positive for indole production, by the methyl red test, and for ornithine decarboxylase; it was negative by the Voges-Proskauer test (for acetylmethylcarbinol production), for urea hydrolysis, for arginine dihydrolase, for lysine decarboxylase, for phenylalanine deaminase, for H2S production, and for gelatin hydrolysis. Strain FRCl was capable of using O2, NO3 −, S2O3 2−, fumarate, and malate as terminal electron acceptors and of reducing U(VI) in the cell suspension. Analysis of the 16S rDNA sequence of the isolate indicated that this strain was 96.4% similar to Salmonella bongori and 96.3% similar to Enterobacter cloacae. Physiological and phylogenetic analyses suggested that strain FRCl belongs to the genus Salmonella and represents a new species, Salmonella subterranea sp. nov.

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