scholarly journals High Throughput Sample Preparation and Analysis for DNA Sequencing, PCR and Combinatorial Screening of Catalysis Based on Capillary Array Technique

2000 ◽  
Author(s):  
Yonghua Zhang
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
High Throughput ◽  
Combinatorial Screening ◽  
Capillary Array

Factors to be considered for robust high-throughput automated DNA sequencing using a multiple-capillary array instrument

1997 ◽  
Author(s):  
Emanuel Carrilho ◽  
Arthur W. Miller ◽  
Marie C. Ruiz-Martinez ◽  
Lev Kotler ◽  
Jeffrey Kesilman ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
High Throughput ◽  
Automated Dna Sequencing ◽  
Capillary Array

Ultra-high throughput rotary capillary array electrophoresis scanner for fluorescent DNA sequencing and analysis

1999 ◽  
Vol 20 (7) ◽  
pp. 1508-1517 ◽  
Author(s):  
James R. Scherer ◽  
Indu Kheterpal ◽  
Arun Radhakrishnan ◽  
William W. Ja ◽  
Richard A. Mathies
Keyword(s):  
Dna Sequencing ◽  
High Throughput ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

scholarly journals Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Eveline Farias-Hesson ◽  
Jonathan Erikson ◽  
Alexander Atkins ◽  
Peidong Shen ◽  
Ronald W. Davis ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
High Throughput ◽  
Magnetic Beads ◽  
Dna Barcode ◽  
Cost Effective ◽  
Dna Libraries ◽  
Emulsion Pcr ◽  
High Throughput Dna Sequencing ◽  
Sequencing Platforms

Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run. An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction. Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligated fragment libraries. Using a liquid-handling robot in conjunction with Carboxy Terminated Magnetic Beads, we labeled each library sample using a unique 6 bp DNA barcode, which allowed multiplex sample processing and sequencing of 32 libraries in a single run using Applied Biosystems' SOLiD sequencer. We applied our semi-automated pipeline to targeted medical resequencing of nuclear candidate genes in individuals affected by mitochondrial disorders. This novel method is capable of preparing as much as 32 DNA libraries in 2.01 days (8-hour workday) for emulsion PCR/high throughput DNA sequencing, increasing sample preparation production by 8-fold.

Microfabricated Capillary Array Electrophoresis: High-Throughput DNA Sequencing and Polymorphism Analysis

2001 ◽  
pp. 462-464
Author(s):  
Brian M. Paegel ◽  
Charles A. Emrich ◽  
Robert G. Blazej ◽  
Christopher J. Elkin ◽  
James R. Scherer ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
High Throughput ◽  
Polymorphism Analysis ◽  
Capillary Array Electrophoresis ◽  
High Throughput Dna Sequencing ◽  
Capillary Array
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