scholarly journals High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection

2001 ◽  
Author(s):  
Gang Xue
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
Fluorescence Detection ◽  
High Throughput ◽  
Laser Induced Fluorescence ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Laser Induced Fluorescence Detection ◽  
Capillary Array Electrophoresis ◽  
Capillary Array

Study of Stray Light and Cross-Talk of the Capillary Array Electrophoresis

2007 ◽  
Vol 364-366 ◽  
pp. 1128-1132
Author(s):  
Jie Wang ◽  
Li Qiang Wang ◽  
Yan Shi ◽  
Hua Zheng ◽  
Zu Kang Lu
Keyword(s):  
Fluorescence Detection ◽  
Cross Talk ◽  
Laser Scanning ◽  
Detection System ◽  
Laser Induced Fluorescence ◽  
Stray Light ◽  
Laser Induced Fluorescence Detection ◽  
Capillary Array Electrophoresis ◽  
Inner Diameter ◽  
Capillary Array

Capillary array electrophoresis (CAE) is founded with the laser induced fluorescence detection (LIFD) system. The same model as the detection system is simulated in Tracepro and the stray light caused by capillaries is analyzed. The stray light distribution of the observation surface is plotted when the laser scans the different sections of a capillary in the array. The results of simulation indicate that the stray light is strongest when scanning the inner center of a capillary and decreases at edges; in two cases (50μm and 75μm inner diameter, ID) when the ID is larger, the stray light is stronger as a whole. Different ID of capillaries which affects the stray light is analyzed; considering many factors, 50μm ID of capillary is appropriate. Based on the analysis of stray light cross-talk by other capillaries in an array, the spacing between capillaries in an array is suggested more than 50μm which the stray light is steady-going and simple to operate. The results can be helpful for the mode of the laser scanning different capillaries in an array sequentially.

Evaluation of the Potential of a Charge-Injection Device for DNA Sequencing by Multiplexed Capillary Electrophoresis

1995 ◽  
Vol 49 (6) ◽  
pp. 825-833 ◽  
Author(s):  
Qingbo Li ◽  
Edward S. Yeung
Keyword(s):  
Capillary Electrophoresis ◽  
Dna Sequencing ◽  
Fluorescence Detection ◽  
Charge Injection ◽  
Laser Induced Fluorescence ◽  
Array Detector ◽  
Injection Device ◽  
Laser Induced Fluorescence Detection ◽  
Array Detectors ◽  
Multiplexed Capillary Electrophoresis

Despite the rapid growth in the use of imaging detectors in spectroscopy, the charge-injection device (CID) has unique features that have not been fully exploited. The advantages of the CID as a two-dimensional array detector for laser-induced fluorescence detection in highly multiplexed capillary electrophoresis are evaluated. In such a system, the CID maintains both high sensitivity and high sampling rate, which are usually difficult to achieve simultaneously with other array detectors. Applying the electronic windowing function significantly improves the scan rate and greatly reduces the volume of data generated. With 1-s exposure time and 488-nm excitation, the detection limit of the system is 10−12 M fluorescein with the device cryogenically cooled and 10−11 M fluorescein at ambient temperature. The low dark current of the CID imager allows operation at room temperature without significantly affecting sensitivity when combined with moderate laser powers. We demonstrate that the CID is well suited for high-speed, high-throughput DNA sequencing based on multiplexed capillary electrophoresis with on-column laser-induced fluorescence detection.

Ultra-high throughput rotary capillary array electrophoresis scanner for fluorescent DNA sequencing and analysis

1999 ◽  
Vol 20 (7) ◽  
pp. 1508-1517 ◽  
Author(s):  
James R. Scherer ◽  
Indu Kheterpal ◽  
Arun Radhakrishnan ◽  
William W. Ja ◽  
Richard A. Mathies
Keyword(s):  
Dna Sequencing ◽  
High Throughput ◽  
Capillary Array Electrophoresis ◽  
Capillary Array
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