scholarly journals PROTON NUCLEAR MAGNETIC RESONANCE (H NMR) OF GLYCOLATE IN REAL WASTE: DEVELOPING AND TESTING ANALYTICAL METHODS FOR THE SAVANNAH RIVER SITE LIQUID WASTE SYSTEM

2021 ◽  
Author(s):  
THOMAS WHITE ◽  
FERNANDO FONDEUR ◽  
CHARLES COLEMAN ◽  
CECILIA DIPRETE ◽  
BRIAN LOONEY
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Analytical Methods ◽  
Liquid Waste ◽  
Savannah River Site ◽  
Proton Nuclear Magnetic Resonance ◽  
Savannah River ◽  
H Nmr ◽  
River Site ◽  
Nuclear Magnetic

scholarly journals Correlations between Proton Nuclear Magnetic Resonance Imaging and Retrospective Histochemical Images in Experimental Cerebral Infarction

1985 ◽  
Vol 5 (2) ◽  
pp. 267-274 ◽  
Author(s):  
Hiroyuki Kato ◽  
Kyuya Kogure ◽  
Hitoshi Ohtomo ◽  
Muneshige Tobita ◽  
Shigeru Matsui ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Spin Echo ◽  
Proton Nuclear Magnetic Resonance ◽  
Nmr Imaging ◽  
Resonance Imaging ◽  
H Nmr ◽  
Experimental Cerebral Infarction ◽  
Nuclear Magnetic

Evaluation of ischemic brain injury in experimental cerebral infarction in gerbils and rats was performed by means of both proton nuclear magnetic resonance imaging ([1H]NMR-CT) and various histochemical analyses. In vivo nuclear magnetic resonance (NMR) imaging was carried out employing saturation recovery, inversion recovery, and spin echo pulse sequences. Spatial resolution of the images was excellent. The ischemic lesions were detected with a remarkable contrast in inversion recovery and spin echo images within a few hours after insult. Those changes in NMR images consistently corresponded with the various retrospective histochemical observations, especially with methods related to brain edema (K+ staining) rather than structural (enzymatic) studies. Calculated T1 and T2 relaxation times indicated the evolution of the edema state in the brain in situ. They correlated excellently with the retrospective water content measurement. As a result, detailed characterization of the edema state induced by cerebral ischemia was possible in vivo using [1H]NMR imaging.

scholarly journals 1H Nuclear Magnetic Resonance: A Future Approach to the Metabolic Profiling of Psychedelics in Human Biofluids?

2021 ◽  
Vol 12 ◽  
Author(s):  
Sylvana Vilca-Melendez ◽  
Malin V. Uthaug ◽  
Julian L. Griffin
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Metabolic Profiling ◽  
Proton Nuclear Magnetic Resonance ◽  
Future Directions ◽  
H Nmr ◽  
Nuclear Magnetic ◽  
Psychedelic Research

While psychedelics may have therapeutic potential for treating mental health disorders such as depression, further research is needed to better understand their biological effects and mechanisms of action when considering the development of future novel therapy approaches. Psychedelic research could potentially benefit from the integration of metabonomics by proton nuclear magnetic resonance (1H NMR) spectroscopy which is an analytical chemistry-based approach that can measure the breakdown of drugs into their metabolites and their metabolic consequences from various biofluids. We have performed a systematic review with the primary aim of exploring published literature where 1H NMR analysed psychedelic substances including psilocin, lysergic acid diethylamide (LSD), LSD derivatives, N,N-dimethyltryptamine (DMT), 5-methoxy-N,N-dimethyltryptamine (5-MeO-DMT) and bufotenin. The second aim was to assess the benefits and limitations of 1H NMR spectroscopy-based metabolomics as a tool in psychedelic research and the final aim was to explore potential future directions. We found that the most current use of 1H NMR in psychedelic research has been for the structural elucidation and analytical characterisation of psychedelic molecules and that no papers used 1H NMR in the metabolic profiling of biofluids, thus exposing a current research gap and the underuse of 1H NMR. The efficacy of 1H NMR spectroscopy was also compared to mass spectrometry, where both metabonomics techniques have previously shown to be appropriate for biofluid analysis in other applications. Additionally, potential future directions for psychedelic research were identified as real-time NMR, in vivo1H nuclear magnetic resonance spectroscopy (MRS) and 1H NMR studies of the gut microbiome. Further psychedelic studies need to be conducted that incorporate the use of 1H NMR spectroscopy in the analysis of metabolites both in the peripheral biofluids and in vivo to determine whether it will be an effective future approach for clinical and naturalistic research.

Synthesis of new 3H-chromeno[2,3-d]pyrimidine-4,6(5H,7H)-diones via the tandem intramolecular Pinner/Dimroth rearrangement

2018 ◽  
Vol 24 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Nasrin Karimi ◽  
Abolghasem Davoodnia ◽  
Mehdi Pordel
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Proton Nuclear Magnetic Resonance ◽  
Phosphoryl Chloride ◽  
Dimroth Rearrangement ◽  
H Nmr ◽  
High Yields ◽  
Aliphatic Carboxylic Acids ◽  
C Nmr ◽  
Nuclear Magnetic

Abstract The reaction of 2-amino-4-aryl-7,7-dimethyl-5-oxo-5,6,7,8-tetrahydro-4H-chromene-3-carbonitriles with excess aliphatic carboxylic acids in the presence of phosphoryl chloride (POCl3) afforded new 2-alkyl-5-aryl-8,8-dimethyl-8,9-dihydro-3H-chromeno[2,3-d]pyrimidine-4,6(5H,7H)-diones in high yields. The suggested mechanism involves a tandem intramolecular Pinner/Dimroth rearrangement. The synthesized compounds were characterized by infrared (IR), proton nuclear magnetic resonance (1H NMR), carbon-13 nuclear magnetic resonance (13C NMR) and elemental analysis.

Serum analysis by1H Nuclear Magnetic Resonance spectroscopy: a new tool for distinguishing neuromyelitis optica from multiple sclerosis

2013 ◽  
Vol 20 (5) ◽  
pp. 558-565 ◽  
Author(s):  
F-M Moussallieh ◽  
K Elbayed ◽  
JB Chanson ◽  
G Rudolf ◽  
M Piotto ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Neuromyelitis Optica ◽  
Demyelinating Diseases ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Background:Neuromyelitis optica (NMO) and multiple sclerosis (MS), two inflammatory demyelinating diseases, are characterized by different therapeutic strategies. Currently, the only biological diagnostic tool available to distinguish NMO from MS is the specific serum autoantibody that targets aquaporin 4, but its sensitivity is low.Objective:To assess the diagnostic accuracy of metabolomic biomarker profiles in these two neurological conditions, compared to control patients.Methods:We acquired serum spectra (47 MS, 44 NMO and 42 controls) using proton nuclear magnetic resonance (1H-NMR) spectroscopy. We used multivariate pattern recognition analysis to identify disease-specific metabolic profiles.Results:The1H-NMR spectroscopic analysis evidenced two metabolites, originating probably from astrocytes, scyllo-inositol and acetate, as promising serum biomarkers of MS and NMO, respectively. In 87.8% of MS patients, scyllo-inositol increased 0.15 to 3-fold, compared to controls and in 74.3% of NMO patients, acetate increased 0.4 to 7-fold, compared to controls. Using these two metabolites simultaneously, we can discriminate MS versus NMO patients (sensitivity, 94.3%; specificity, 90.2%).Conclusion:This study demonstrates the potential of1H-NMR spectroscopy of serum as a novel, promising analytical tool to discriminate populations of patients affected by NMO or MS.

scholarly journals Metabolomics comparison of rumen fluid and milk in dairy cattle using proton nuclear magnetic resonance spectroscopy

2021 ◽  
Vol 34 (2) ◽  
pp. 213-222
Author(s):  
Jun Sik Eom ◽  
Eun Tae Kim ◽  
Hyun Sang Kim ◽  
You Young Choi ◽  
Shin Ja Lee ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Dairy Cattle ◽  
Metabolic Diseases ◽  
Rumen Fluid ◽  
Proton Nuclear Magnetic Resonance ◽  
Resonance Spectroscopy ◽  
H Nmr ◽  
Nuclear Magnetic

Objective: The metabolites that constitute the rumen fluid and milk in dairy cattle were analyzed using proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy and compared with the results obtain for other dairy cattle herds worldwide. The aim was to provide basic dataset for facilitating research on metabolites in rumen fluid and milk.Methods: Six dairy cattle were used in this study. Rumen fluid was collected using a stomach tube, and milk was collected using a pipeline milking system. The metabolites were determined by <sup>1</sup>H-NMR spectroscopy, and the obtained data were statistically analyzed by principal component analysis, partial least squares discriminant analysis, variable importance in projection scores, and metabolic pathway data using Metaboanalyst 4.0.Results: The total numbers of metabolites in rumen fluid and milk were measured to be 186 and 184, and quantified as 72 and 109, respectively. Organic acid and carbohydrate metabolites exhibited the highest concentrations in rumen fluid and milk, respectively. Some metabolites that have been associated with metabolic diseases (acidosis and ketosis) in cows were identified in rumen fluid, and metabolites associated with ketosis, somatic cell production, and coagulation properties were identified in milk.Conclusion: The metabolites measured in rumen fluid and milk could potentially be used to detect metabolic diseases and evaluate milk quality. The results could also be useful for metabolomic research on the biofluids of ruminants in Korea, while facilitating their metabolic research.

scholarly journals Nuclear Magnetic Resonance-based Metabolomics of Blood Plasma from Dairy Calves Infected with the Main Causal Agents of Bovine Respiratory Disease (BRD)

2021 ◽  
Author(s):  
Mariana Santos-Rivera ◽  
Nicholas C. Fitzkee ◽  
Rebecca A. Hill ◽  
Richard E. Baird ◽  
Ellianna Blair ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Blood Plasma ◽  
Respiratory Disease ◽  
Nmr Spectra ◽  
Bovine Respiratory Disease ◽  
Dairy Calves ◽  
Proton Nuclear Magnetic Resonance ◽  
H Nmr ◽  
Nuclear Magnetic

Abstract Each year, Bovine Respiratory Disease (BRD) results in significant economic loss in the cattle sector, and novel metabolic profiling and early diagnosis techniques represent a promising tool for developing effective measures for disease management. Here, proton - Nuclear Magnetic Resonance (1H - NMR) spectra were used to characterize metabolites from blood plasma collected from dairy calves intentionally infected with the main BRD causal agents, bovine respiratory syncytial virus (BRSV) and Mannheimia haemolytica (MH), to generate a well-defined metabolomic profile under controlled conditions. In response to infection, 42 metabolites (BRSV = 27, MH = 24) changed in concentration compared to the Baseline (non-infected) state. Fuel substrates and products exhibited a particularly strong effect, reflecting imbalances that occur during the immune response. Glucose levels decreased only during bacterial infection, suggesting that the clinical signs of bacterial BRD are more energetically taxing than those of viral BRD. Furthermore, 1H - NMR spectra from Baseline and Infected samples were discriminated with an accuracy, sensitivity, and specificity ≥ 95% using chemometrics to model the changes associated with disease, suggesting that metabolic profiles can be used for further development and validation of diagnostic tools.

Synchrotron powder diffraction, X-ray absorption and 1H nuclear magnetic resonance data for hypoxanthine, C5H4N4O

2015 ◽  
Vol 30 (3) ◽  
pp. 278-285 ◽  
Author(s):  
Joel Reid ◽  
Toby Bond ◽  
Shiliang Wang ◽  
Jigang Zhou ◽  
Anguang Hu
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Proton Nuclear Magnetic Resonance ◽  
Keto Form ◽  
Purine Base ◽  
X Ray ◽  
H Nmr ◽  
Synchrotron Powder Diffraction ◽  
X Ray Absorption ◽  
Nuclear Magnetic

Synchrotron powder X-ray diffraction, X-ray absorption spectroscopy (XAS), and proton nuclear magnetic resonance (1H-NMR) data have been used to examine the structure of hypoxanthine, 1,7-dihydro-6H-purin-6-one (C5H4N4O), a purine base that participates in numerous metabolic processes. XAS and 1H-NMR spectroscopy were used to determine that hypoxanthine was present in its keto form (both in solid state and dissolved in an organic solvent). Rigid body refinement was performed with the Rietveld software package GSAS yielding triclinic lattice parameters of a = 7.1179 (2) Å, b = 9.7830 (3) Å, c = 10.4009 (3) Å, α = 58.876 (1)°, β = 67.609 (1)°, and γ = 71.937 (2)° (C5H4N4O, Z = 4, space group P$\bar 1$).

Sign in / Sign up

Export Citation Format

Share Document