scholarly journals CHANGES IN THE ELECTRICAL SURFACE CHARGE AND TRANSPLANTATION PROPERTIES OF TA3 ASCITES TUMOR CELLS DURING SHORT-TERM MAINTENANCE IN AN ISOTONIC SALT SOLUTION

1980 ◽  
Author(s):  
T. S. Tenforde ◽  
W. R. Richards ◽  
L. S. Kelly
Keyword(s):  
Surface Charge ◽  
Tumor Cells ◽  
Salt Solution ◽  
Ascites Tumor ◽  
Short Term ◽  
Term Maintenance ◽  
Isotonic Salt Solution

Alteration by prolactin of surface charge and membrane fluidity of rat 13762 mammary ascites tumor cells

1984 ◽  
Vol 6 (1) ◽  
pp. 53-66 ◽  
Author(s):  
David A. Zarkower ◽  
Lindsay D. Plank ◽  
Elaine Kunze ◽  
Alec Keith ◽  
Paul Todd ◽  
...  
Keyword(s):  
Surface Charge ◽  
Membrane Fluidity ◽  
Tumor Cells ◽  
Ascites Tumor

The release of nucleic acids and protein from ascites tumor cells incubated with glycerol, dimethylsulfoxide, and inositol

1969 ◽  
Vol 47 (9) ◽  
pp. 871-876 ◽  
Author(s):  
C. P. Eng ◽  
J. F. Morgan
Keyword(s):  
Sodium Chloride ◽  
Nucleic Acids ◽  
Dimethyl Sulfoxide ◽  
Tumor Cells ◽  
Incubation Period ◽  
Salt Solution ◽  
Ascites Tumor ◽  
Cryoprotective Agents ◽  
Mouse Ascites ◽  
Control Fluid

The effect of the cryoprotective agents glycerol, dimethyl sulfoxide (DMSO), and inositol was studied on Ehrlich and 6C3HED mouse ascites tumor cells. A marked release of both nucleic acids and protein was caused by incubation for 1 h in glycerol or DMSO, an observation which suggested cell disruption. The osmolarity of ascites tumor fluid was found to be 300 ± 10 milliosmoles, a level corresponding to 2.1% DMSO, 2.2% glycerol, and 5.4% inositol. Incubation in isotonic solutions of DMSO and glycerol caused a marked release of both nucleic acids and protein. In contrast, incubation with isotonic inositol caused a release of nucleic acids but essentially no protein beyond the amount released in the control fluid of Hanks' balanced salt solution. In all cases, the release of intracellular materials was influenced by age of the tumor cells and the temperature of incubation.The specific release of nucleic acids in the presence of inositol was found to occur to greatest extent early in the incubation period. This release could be inhibited by sodium chloride.

scholarly journals STUDIES ON CELL DEFORMABILITY

1965 ◽  
Vol 26 (3) ◽  
pp. 735-739 ◽  
Author(s):  
Leonard Weiss
Keyword(s):  
Potential Energy ◽  
Surface Charge ◽  
Tumor Cells ◽  
Radius Of Curvature ◽  
Cell Periphery ◽  
Charge Reduction ◽  
Ascites Tumor ◽  
Cell Deformability ◽  
Sarcoma 37 ◽  
Cellular Deformability

The deformability of the surface membranes of Sarcoma 37 and Ehrlich murine ascites tumor cells was assessed by the pressure required to suck a hemispherical bulge from these cells into a micropipette. It was shown that treatment with neuraminidase allowed the cells to be deformed with significantly less suction, and that enzymatic treatment also produced a significant reduction in surface charge as determined by measurement of cellular electrophoretic mobility. It is suggested that the increase in cellular deformability may be related to charge reduction, and that the charge at the cell periphery may affect not only the magnitude of the potential energy barriers hindering contact between cells, but also the ease with which cells can form low radius of curvature probes in order to help overcome these barriers.

scholarly journals Ribonucleic Acids in Virus-infected and Uninfected Krebs II Ascites Tumor Cells

1963 ◽  
Vol 238 (12) ◽  
pp. 3978-3984
Author(s):  
R. Eason ◽  
M.J. Cline ◽  
R.M.S. Smellie
Keyword(s):  
Tumor Cells ◽  
Ascites Tumor ◽  
Ribonucleic Acids

scholarly journals Coordination among Rate-limiting Steps of Glycolysis and Respiration in Intact Ascites Tumor Cells

1967 ◽  
Vol 242 (9) ◽  
pp. 2021-2028
Author(s):  
In-Young Lee ◽  
Robert C. Strunk ◽  
Elmon L. Coe
Keyword(s):  
Tumor Cells ◽  
Ascites Tumor ◽  
Rate Limiting

scholarly journals Novikoff ascites tumor cells contain N-acetyllactosaminide beta 1 leads to 3 and beta 1 leads to 6 N-acetylglucosaminyltransferase activity.

1983 ◽  
Vol 258 (6) ◽  
pp. 3435-3437 ◽  
Author(s):  
D H van den Eijnden ◽  
H Winterwerp ◽  
P Smeeman ◽  
W E Schiphorst
Keyword(s):  
Tumor Cells ◽  
Ascites Tumor
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