scholarly journals STUDIES ON CELL DEFORMABILITY

1965 ◽  
Vol 26 (3) ◽  
pp. 735-739 ◽  
Author(s):  
Leonard Weiss
Keyword(s):  
Potential Energy ◽  
Surface Charge ◽  
Tumor Cells ◽  
Radius Of Curvature ◽  
Cell Periphery ◽  
Charge Reduction ◽  
Ascites Tumor ◽  
Cell Deformability ◽  
Sarcoma 37 ◽  
Cellular Deformability

The deformability of the surface membranes of Sarcoma 37 and Ehrlich murine ascites tumor cells was assessed by the pressure required to suck a hemispherical bulge from these cells into a micropipette. It was shown that treatment with neuraminidase allowed the cells to be deformed with significantly less suction, and that enzymatic treatment also produced a significant reduction in surface charge as determined by measurement of cellular electrophoretic mobility. It is suggested that the increase in cellular deformability may be related to charge reduction, and that the charge at the cell periphery may affect not only the magnitude of the potential energy barriers hindering contact between cells, but also the ease with which cells can form low radius of curvature probes in order to help overcome these barriers.

scholarly journals STUDIES ON CELL DEFORMABILITY

1966 ◽  
Vol 30 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Leonard Weiss
Keyword(s):  
Electrophoretic Mobility ◽  
Proteolytic Enzymes ◽  
The Other ◽  
Cell Periphery ◽  
Cell Deformability ◽  
Other Hand ◽  
Sarcoma 37 ◽  
Cellular Deformability ◽  
Murine Sarcoma

Murine sarcoma 37 ascites cells were treated with the proteolytic enzymes, trypsin and chymotrypsin, after which cellular deformability and electrophoretic mobility were measured. It was shown that incubation with trypsin increased the ease with which the cells could be deformed without changing electrophoretic mobility, and that diisopropylfluorophosphate (DFP)-trypsin was inactive, a fact which suggests that trypsin-sensitive peptide linkages help to maintain the "tension" at the cell periphery. On the other hand, chymotrypsin reduced cellular electrophoretic mobility without appreciably altering deformability. This suggests that, although chymotrypsin-sensitive bonds do not contribute to "tension," they are in some way associated with charged groups at the cell periphery.

Alteration by prolactin of surface charge and membrane fluidity of rat 13762 mammary ascites tumor cells

1984 ◽  
Vol 6 (1) ◽  
pp. 53-66 ◽  
Author(s):  
David A. Zarkower ◽  
Lindsay D. Plank ◽  
Elaine Kunze ◽  
Alec Keith ◽  
Paul Todd ◽  
...  
Keyword(s):  
Surface Charge ◽  
Membrane Fluidity ◽  
Tumor Cells ◽  
Ascites Tumor

scholarly journals STUDIES ON CELL DEFORMABILITY

1967 ◽  
Vol 33 (2) ◽  
pp. 341-347 ◽  
Author(s):  
Leonard Weiss
Keyword(s):  
Cell Types ◽  
Mechanical Effect ◽  
Cell Periphery ◽  
Detectable Change ◽  
Anionic Sites ◽  
Cell Deformability ◽  
Edta Treatment ◽  
Edta Solution ◽  
Hydrodynamic Slip ◽  
Sarcoma 37

The nonlethal procedure of incubation in EDTA solution makes the peripheral regions of ascites sarcoma 37 cells more easily deformable, as reflected in measurements of the decreased amount of negative pressure required to suck out standard hemispherical bulges from the cells into micropipettes. The facilitation of deformability was abolished after reincubation of cells in calcium-containing saline, and this mechanical parameter was partially restored to normal after reincubation in magnesium-containing saline; the mechanical effect of EDTA treatment is, therefore, thought to be due mainly to the removal of calcium from the cell periphery. As EDTA treatment produces no detectable change in cellular electrophoretic mobility, it is concluded that peripheral calcium must be bound to anionic sites deeper than about 10 A from the cellular hydrodynamic slip plane. The data are discussed with emphasis on the view that they should not be extrapolated freely to other cell types.

scholarly journals Ribonucleic Acids in Virus-infected and Uninfected Krebs II Ascites Tumor Cells

1963 ◽  
Vol 238 (12) ◽  
pp. 3978-3984
Author(s):  
R. Eason ◽  
M.J. Cline ◽  
R.M.S. Smellie
Keyword(s):  
Tumor Cells ◽  
Ascites Tumor ◽  
Ribonucleic Acids

scholarly journals Coordination among Rate-limiting Steps of Glycolysis and Respiration in Intact Ascites Tumor Cells

1967 ◽  
Vol 242 (9) ◽  
pp. 2021-2028
Author(s):  
In-Young Lee ◽  
Robert C. Strunk ◽  
Elmon L. Coe
Keyword(s):  
Tumor Cells ◽  
Ascites Tumor ◽  
Rate Limiting
Sign in / Sign up

Export Citation Format

Share Document