scholarly journals Antioxidant Effects of Red Fruit Oil on MMP-1 Gene Expression and Malondialdehyde Levels on Skin Exposed to UVB Rays

2019 ◽  
Vol 3 (2) ◽  
pp. 100
Author(s):  
Monita Sugianto ◽  
Achadiyani Achadiyani ◽  
Gaga Irawan Nugraha
Keyword(s):  
Chronic Exposure ◽  
Wistar Rats ◽  
Beta Carotene ◽  
Oxygen Species ◽  
Not Given ◽  
Male Wistar Rats ◽  
Ros Formation ◽  
Fruit Oil ◽  
Antioxidant Effects ◽  
Β Carotene

Background: Chronic exposure ultraviolet (UV)-B radiation causes reactive oxygen species (ROS) formation. Furthermore, ROS will induce the formation of malondialdehyde and increase matrix metalloproteinase (MMP)-1 expression. One strategy against the free radicals effects is by consuming antioxidants. This study aims to analyze the antioxidants effect of red fruit oil (RFO) on MMP-1 expression and malondialdehyde levels due to exposure to UVB rays.Materials and Methods: Thirty male Wistar rats were divided into 5 groups. The P0 group was not given treatment, the P1 group was only exposed to UVB light, the P2 group was exposed to UVB light and given 0.5 mL/200 g body weight (BW) of RFO, the P3 group was exposed to UVB light and given 1 mL/200 g BW of RFO, and group P4 exposed to UVB rays and given 2 mL/200 g BW of RFO. Experimental animals would be examined for MMP-1 expression and malondialdehyde level. RFO would be identified with β-carotene and tocopherol content.Results: Beta-carotene and tocopherol were detected in RFO. RFO reduced significantly MMP-1 expression (p<0.05) in P2 group (0.73±1.27), P3 group (0.63±0.95), P4 group (9.56±20.97) compared group P1 (48.07±65.58). However, RFO did not reduce malondialdehyde levels (p>0.05).Conclusion: Our research demonstrates RFO containing tocopherol and β-carotene can reduce the MMP-1 expression, but does not affect malondialdehyde levels due to exposure to UVB rays. An effective dose that can reduce malondialdehyde levels and MMP-1 expression is 1 mL/200 g BW.Keywords: red fruit oil, antioxidant, skin, MMP-1 expression, malondialdehyde, UVB rays, photoaging 

scholarly journals Reproductive effects of the psychoactive beverage ayahuasca in male Wistar rats after chronic exposure

2017 ◽  
Vol 27 (3) ◽  
pp. 353-360 ◽  
Author(s):  
Alana de Fátima Andrade Santos ◽  
Ana Luiza Sarkis Vieira ◽  
Aline Pic-Taylor ◽  
Eloisa Dutra Caldas
Keyword(s):  
Chronic Exposure ◽  
Wistar Rats ◽  
Reproductive Effects ◽  
Male Wistar Rats

scholarly journals Implication of Acute, Sub-chronic and Chronic Exposure to Different Pesticides via Inhalation on Male Wistar Rats

2017 ◽  
Vol 5 (4) ◽  
pp. 74-85
Author(s):  
Oluwatoyin Taiwo Adeoti ◽  
Donatus Chuka Belonwu ◽  
Mathew Owhonda Wegwu ◽  
Justice Obinna Osuoha
Keyword(s):  
Chronic Exposure ◽  
Wistar Rats ◽  
Male Wistar Rats

scholarly journals Rutin Attenuates Acrylamide Induced Neuropathic Pain via Inhibition of Proinflammatory Cytokines, Up-Regulation of Bcl-2 and Down-Regulation of Bax

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Vineela Sathuluri ◽  
Thakur Santh Rani
Keyword(s):  
Wistar Rats ◽  
Neuronal Degeneration ◽  
Oxygen Species ◽  
Toxic Chemical ◽  
Stress Markers ◽  
Neurological Score ◽  
Tnf Α ◽  
Ameliorative Effect ◽  
Male Wistar Rats ◽  
Passion Flower

Rutin is a flavonoid of the flavonol type found in many typical plants, such as buckwheat, passion flower, apple and tea. Acrylamide (ACR) is a known industrial toxic chemical that produces neurotoxicity characterized by progressive neuronal degeneration. Rats were randomly divided into Control, ACR, Pregabalin and Rutin treated groups. Male wistar rats were treated with ACR (50 mg/kg/ i.p.) for 4 weeks which produce typical symptoms of neuropathy in rats. Pregabalin (10 mg/kg) and Rutin (50 & 100 mg/kg) were administered orally for 4 weeks after one hour of ACR administration. ACR enhanced the production of reactive oxygen species (ROS). Treatment with Rutin significantly improved neurological score. Rutinsignificantly (p<0.001) attenuated acrylamide induced oxidative stress markers. The expression of Bcl-2 was up-regulated and TNF-α, IL-6 and Bax were down-regulated by rutin treatment. From our results, it can be concluded that rutinshowed an ameliorative effect against ACR induced neurotoxicity in rats through its antioxidant, anti-inflammatory and antiapoptotic actions.

Terbufos sulfone aggravates kidney damage in STZ-induced diabetic rats

Biologia ◽  
2017 ◽  
Vol 72 (8) ◽  
Author(s):  
Syed Muhammad Nurulain ◽  
Shreesh Ojha ◽  
Mohamed Shafiullah ◽  
Javed Yasin ◽  
Tayyaba Yasmin ◽  
...  
Keyword(s):  
Body Weight ◽  
Chronic Exposure ◽  
Wistar Rats ◽  
Organophosphorus Compounds ◽  
Lethal Dose ◽  
Diabetic Rats ◽  
Kidney Damage ◽  
Male Wistar Rats ◽  
The Impact

AbstractThe consequences of chronic exposure of organophosphorus compounds (OPCs) on diabetic subjects have been seldom reported. The aim of the present study was to assess the impact of non-lethal dose of terbufos sulfone (TS), an organophosphate, on the kidney of non-diabetic and streptozotocin (STZ)- induced diabetic rats. The diabetogenic effect of TS was also examined. Male Wistar rats were treated for two weeks with 130 µg/kg body weight/day of TS. This dose was 1/20 of LD

Effects of sub-chronic exposure to palladium (as potassium hexachloro-palladate) on cytokines in male Wistar rats

2008 ◽  
Vol 27 (6) ◽  
pp. 493-497 ◽  
Author(s):  
I Iavicoli ◽  
G Carelli ◽  
A Marinaccio ◽  
L Fontana ◽  
EJ Calabrese
Keyword(s):  
Chronic Exposure ◽  
Wistar Rats ◽  
Statistical Significance ◽  
Response Relationship ◽  
Dose Response Relationship ◽  
Mean Values ◽  
Male Wistar Rats ◽  
The Difference

Palladium (Pd) is a heavy metal belonging to the platinum group elements. It has been shown that Pd and its compounds can cause sensitization, asthma and dermatologic disorders. In this study, Wistar rats were exposed for 3 months to 0, 1, 10, 100, and 250 ng/ml of Pd (as potassium hexachloro-palladate) in drinking water. At the end of exposure, possible changes in two type-1 cytokines [interleukin (IL)-2, interferon (INF)-γ] and one type-2 cytokine (IL-4) in the serum were measured. After the sub-chronic exposure, analysis of variance of IL-2 and INF-γ response shows that the difference between mean values in the groups of animals exposed reaches statistical significance for IL-2 ( P = 0.001), showing a J-shaped dose–response relationship. At the higher dose of 250 ng/ml Pd, it was observed a significant increase in IL-2 production when compared with the controls. Furthermore at 1 and 10 ng/ml of Pd we observed an increase of INF-γ production that was significant at 100 ng/ml of Pd, while at the higher dose of 250 ng/ml of Pd the response was indistinguishable from the control. At the doses investigated, Pd has been shown not to determine a modification of IL-4 response ( P = 0.10). These preliminary findings indicate the need to carry out further investigations regarding the effect of other Pd compounds and the measurement of other types of cytokines both in the animal model used in our study and other models.

scholarly journals Hepatic and reproductive toxicity of sub-chronic exposure to dichlorvos and lead acetate on male Wistar rats

2020 ◽  
Vol 9 (6) ◽  
pp. 283
Author(s):  
WahabAdekunle Oyeyemi ◽  
Oore-oluwapoOlolade Daramola ◽  
AdeniranOluwadamilare Akinola ◽  
AdeoyeOyewole Idris ◽  
Ikponmwosa Aikpitanyi
Keyword(s):  
Lead Acetate ◽  
Chronic Exposure ◽  
Wistar Rats ◽  
Reproductive Toxicity ◽  
Male Wistar Rats

scholarly journals Pemberian Ekstrak Daun Gandarusa (Justicia gendarussa Burm. f.) Menurunkan Kadar F2-isoprostan Urin Tikus Wistar (Rattus norvegicus) Jantan yang Diinduksi Latihan Fisik Berlebih

2017 ◽  
Vol 9 (3) ◽  
Author(s):  
DG Ika Akpriyanti ◽  
Wimpie Pangkahila ◽  
IGM Aman
Keyword(s):  
Treatment Group ◽  
Physical Training ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Oxygen Species ◽  
Post Test ◽  
Male Wistar Rats ◽  
The Mean

Abstract: Excessive physical activity increases the consumption of oxygen which can result in increased levels of reactive oxygen species (ROS) causing oxidative stress characterized by the elevation of F2-isoprostane as a byproduct of lipid peroxidation. Willow (Justicia gendarussa Burm. f.) has an antioxidant activity that is able to inhibit and break the chain reaction of free radicals and prevent lipid peroxidation. This study was aimed to prove that willow leaf extract could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training. This was a true experimental study using 16 male Wistar rats (Rattus norvegicus), aged 2-3 months, weighing 180-200 g as subjects. The control group (P0) was treated with a placebo and excessive physical training, and the treatment group (P1) was treated with the willow leaf extract of 200mg/rat/day and excessive physical training. Before and after 14 days of treatment, the urine samples were collected and their levels of F2-isoprostane 8-isoPGF2α were examined by using enzyme immunoassay kit (EIA). The results showed that the mean level of F2-isoprostane in the control group (P0) before treatment (pretest) was 5.20±0.483 ng / mL while in the treatment group (P1) was 5.46±0.655 ng / mL (P > 0.05). On the other hand, after treatment for 7 days (post-test), the mean level of F2-isoprostane in the control group (P0) was 5.34±0.476 ng/mL and in the treatment group (P1) was 3.61±0.389 ng/mL (P < 0.01). Conclusion: The extract of willow (Justicia gendarussa Burm. f.) leaf could inhibit the elevation of F2-isoprostane levels in male Wistar rats (Rattus norvegicus) urin induced by excessive physical training.Keywords: willow leaf, F2-isoprostane, excessive physical trainingAbstrak: Aktivitas fisik berlebih akan meningkatkan komsumsi oksigen yang dapat mengakibatkan peningkatan kadar reactive oxygen species (ROS) sehingga menyebabkan stres oksidatif yang ditandai dengan meningkatnya F2-isoprostan sebagai salah satu produk peroksidasi lipid. Tanaman gandarusa (Justicia gendarussa Burm. f.) mengandung antioksidan yang dapat mencegah reaksi peroksidasi lipid. Penelitian ini bertujuan untuk membuktikan bahwa pemberian ekstrak daun gandarusa dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih. Jenis penelitian ialah eksperimental dengan subjek 16 ekor tikus (Rattus norvegicus), galur Wistar, sehat, berumur 6 bulan, berat badan 180-200 gr, dengan kadar F2-isoprostan >2ng/mL. Kelompok kontrol (P0) diberikan pelatihan fisik berlebih dan plasebo aquadest 2ml sedangkan kelompok perlakuan (P1) diberikan pelatihan fisik berlebih dan ekstrak daun gandarusa dosis 200mg/ekor/hari. Sebelum dan setelah 14 hari perlakuan, sampel urin ditampung dan diperiksa kadar F2-isoprostan menggunakan 8-isoPGF2α enzyme immunoassay kit (EIA) dari assay design untuk data pretest dan post-test. Hasil penelitian menunjukkan rerata kadar F2-isoprostan pada kelompok P0 sebelum perlakuan (pretest) ialah 5,20±0,483 ng/mL, sedangkan pada kelompokP1 ialah 5,46±0,655 ng/mL (P > 0,05). Setelah perlakuan selama 14 hari (post-test), rerata kadar F2-isoprostan pada kelompok P0 ialah 5,34±0,476 ng/mL dan pada kelompok P1 ialah 3,61±0,389 ng/mL (P < 0,01). Simpulan: Ekstrak daun gandarusa (Justicia gendarussa burm. f.) dapat menurunkan kadar F2-isoprostan urin tikus Wistar (Rattus norvegicus) jantan yang diinduksi latihan fisik berlebih.Kata kunci: daun gandarusa, F2-isoprostan, pelatihan fisik berlebih

Diuron exposure induces systemic and organ-specific toxicity following acute and sub-chronic exposure in male Wistar rats

2011 ◽  
Vol 31 (3) ◽  
pp. 387-396 ◽  
Author(s):  
Alexandre Domingues ◽  
Luis Fernando Barbisan ◽  
Priscila Raquel Martins ◽  
Ana Lúcia Tozzi Spinardi-Barbisan
Keyword(s):  
Chronic Exposure ◽  
Wistar Rats ◽  
Organ Specific ◽  
Male Wistar Rats

Further analysis of behavioral and endocrine consequences of chronic exposure of male Wistar rats to subtoxic doses of endocrine disruptor chlorobenzenes

2011 ◽  
Vol 103 (5) ◽  
pp. 421-430 ◽  
Author(s):  
Z. Valkusz ◽  
G. Nagyéri ◽  
M. Radács ◽  
T. Ocskó ◽  
P. Hausinger ◽  
...  
Keyword(s):  
Chronic Exposure ◽  
Endocrine Disruptor ◽  
Wistar Rats ◽  
Male Wistar Rats
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