scholarly journals Stimulation of Bicarbonate Secretion by Luminal Short-Chain Fatty Acid in the Rat and Human Colon In Vitro.

1994 ◽  
Vol 44 (5) ◽  
pp. 519-531 ◽  
Author(s):  
Mizuki DOHGEN ◽  
Hisayoshi HAYAHSHI ◽  
Takaji YAJIMA ◽  
Yuichi SUZUKI
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Human Colon ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Bicarbonate Secretion ◽  
Stimulation Of

Short-chain fatty acid transport and its effects on ion transport by rabbit cecum

1987 ◽  
Vol 253 (2) ◽  
pp. G171-G178
Author(s):  
M. Hatch
Keyword(s):  
Fatty Acid ◽  
Short Chain Fatty Acid ◽  
Short Circuit ◽  
Basolateral Membrane ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Bathing Solution ◽  
Fatty Acid Transport ◽  
Unexpected Finding

Short-chain fatty acid (SCFA) metabolism and transport were examined in vitro across isolated rabbit cecal epithelia whose primary function is absorption of these solutes. This study shows that although there was some low-level metabolism of SCFAs to ketone bodies by the isolated cecum, a significantly higher oxygen consumption was sustained for a longer time period by tissues incubated in glucose-containing salines. The cecum supported a significant net secretory flux of acetate (J net Ac- = -1.13 +/- 0.13 mu eq X cm-2 X h-1) and propionate (J net Pr- = -0.61 +/- 0.14 mu eq X cm-2 X h-1). This study also shows that glucose significantly enhanced short-circuit current (Isc), tissue conductance (Gt), and sodium transport across this tissue. Neither Ac- nor Pr- enhanced net sodium flux (J net Ac-) but Pr- significantly reduced net chloride flux (J net Cl-), whereas Ac- had no effect. The increase in Isc and Gt observed in the presence of SCFAs was attributable to the presence of SCFA in the serosal bathing solution alone. To explain the latter finding and the unexpected finding of SCFA secretion, the existence of an electrogenic anion (HCO3-) secretory pathway is postulated. It is suggested that this system can accommodate SCFAs in vitro and that it is a Na+-dependent system located on the basolateral membrane of the cecal cell.

In Vitro Batch Fecal Fermentation Comparison of Gas and Short-Chain Fatty Acid Production Using “Slowly Fermentable” Dietary Fibers

2011 ◽  
Vol 76 (5) ◽  
pp. H137-H142 ◽  
Author(s):  
Amandeep Kaur ◽  
Devin J. Rose ◽  
Pinthip Rumpagaporn ◽  
John A. Patterson ◽  
Bruce R. Hamaker
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Short Chain Fatty Acid ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Dietary Fibers ◽  
Fatty Acid Production

196 Effect of Exogenous Enzyme Supplementation and Fermentation of Oilseed By-products on in vitro Digestibility and Production of Short Chain Fatty Acid

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 79-79
Author(s):  
Jae-Cheol Jang ◽  
Aqsa Javaid ◽  
Pedro E Urriola ◽  
Gerald C Shurson
Keyword(s):  
Fatty Acid ◽  
Butyric Acid ◽  
Short Chain Fatty Acid ◽  
Gas Production ◽  
Chain Fatty Acid ◽  
Short Chain ◽  
Exogenous Enzyme ◽  
Enzyme Cocktail ◽  
Enzyme Supplementation

Abstract An experiment was conducted to investigate the effect of exogenous enzyme supplementation and solid-state fermentation (SSF) with a mixed bacterial culture on in vitro dry matter digestibility (IVDMD) and short chain fatty acid (SCFA) production of soybean meal (SBM) or rapeseed meal (RSM). A 2 × 2 factorial design was used and included the factors of 1) exogenous enzyme cocktail (supplemented and non-supplemented), 2) microbial fermentation (fermented and non-fermented) applied to SBM or RSM in vitro. The exogenous enzyme cocktail consisted of non-starch polysaccharide (NSP) degrading enzymes (NSP-EZ) with phytase (10,000 FTU/kg), and the SSF were carried out using Bacillus subtilis. The fermented feed ingredients were collected after 48 h incubation at 37oC. Samples were hydrolyzed in two steps using pepsin and pancreatin to calculate IVDMD. Subsequently, the hydrolyzed residues were filtered, dried, and pooled for incubation in a buffered mineral solution with fresh swine feces. Gas production kinetics during fermentation was measured for 72 h and analyzed by fitting data to an exponential model. The fermentation residues were filtered, and the supernatant was analyzed for concentration of SCFA. The IVDMD from simulated gastric and small intestinal hydrolysis was greater (P < 0.01) for SSF in both SBM and RSM. During fermentation, the hydrolysis residue from SBM treated with SSF required less time to reach half asymptote, had greater maximal gas production, and greater fractional degradation (P < 0.01, respectively) compared with non-fermented SBM. The IVDMD from simulated total tract digestion was greater (P < 0.01) for SSF in RSM compared with SBM, while SBM had greater IVDMD for both SSF and NSP-EZ (P < 0.01). Production of butyric acid was greater for SSF (P < 0.01) compared with non-SSF in both SBM and RSM. These results suggest that SSF can improve IVDMD and produce greater amounts of butyric acid compared with NSP-EZ supplementation in SBM and RSM.

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