scholarly journals Hepatic Extraction and Hepatic Action of Insulin, Glucagon, and Epinephrine in Bivascularly Perfused Rat Liver.

1993 ◽  
Vol 43 (3) ◽  
pp. 371-378 ◽  
Author(s):  
Tadasu IKEDA ◽  
Hikari TERASAWA ◽  
Masahiko ISHIMURA ◽  
Hiroshi OCHI ◽  
Katsumi FUJIYAMA ◽  
...  
Keyword(s):  
Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Extraction ◽  
Hepatic Action

Hepatic extraction, metabolism, and biliary excretion of irinotecan in the isolated perfused rat liver

2001 ◽  
Vol 90 (6) ◽  
pp. 722-731 ◽  
Author(s):  
Christian Farabos ◽  
Marie‐Christine Haaz ◽  
Pierre Gires ◽  
Jacques Robert
Keyword(s):  
Rat Liver ◽  
Biliary Excretion ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Extraction

Hepatic extraction of renin: Quantitation and characterization in the isolated perfused rat liver

Hepatology ◽  
1987 ◽  
Vol 7 (6) ◽  
pp. 1254-1261 ◽  
Author(s):  
Joan A. Keiser ◽  
Juan C. Romero ◽  
Louis J. Kost ◽  
Nicholas F. Larusso
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Extraction

Effect of graded bilirubin loads on bilirubin transport by perfused rat liver

1976 ◽  
Vol 230 (3) ◽  
pp. 736-742 ◽  
Author(s):  
Bloomer ◽  
J Zaccaria
Keyword(s):  
Rat Liver ◽  
Extraction Efficiency ◽  
Unconjugated Bilirubin ◽  
Constant Infusion ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Extraction ◽  
Perfusion Medium ◽  
Wide Range ◽  
Male Wistar Rats

Features of hepatic bilirubin transport were studied with the isolated perfused rat liver. Male Wistar rats weighing 350-400 g were used as liver donors. When bilirubin was constantly infused into the perfusion medium, which contained sheep erythrocytes and 3.0 g/100 ml bovine serum albumin, the maximal excretion rate for bilirubin was 14.4 +/- 1.2 mug/min per g liver. Over a wide range of constant bilirubin infusion rates which went as high as 25.9 mug/min per g liver, there was no effect on bile flow, bile acid excretion, or the pattern of bilirubin conjugates in bile. The hepatic extraction efficiency for unconjugated bilirubin from the perfusate also remained constant averaging 26%. However, when bolus injections of bilirubin were used to produce higher levels of unconjugated bilirubin in the perfusate than could be attained during constant infusion, the disappearance rate of [14C]bilirubin from the perfusate decreased with increasing bilirubin concentrations. This was consistent with saturation of the hepatic removal of unconjugated bilirubin.

Effect of intestinal factors on extraction of insulin in perfused rat liver

1987 ◽  
Vol 253 (6) ◽  
pp. E603-E607
Author(s):  
T. Ikeda ◽  
T. Yoshida ◽  
M. Honda ◽  
Y. Ito ◽  
I. Murakami ◽  
...  
Keyword(s):  
Direct Effect ◽  
Rat Liver ◽  
Untreated Control ◽  
Oral Glucose ◽  
Perfused Rat Liver ◽  
Hepatic Extraction ◽  
Rat Intestine ◽  
Isolated Rat Liver ◽  
Glucose Ingestion ◽  
Isolated Rat

To study the direct effect of intestinal factors on hepatic extraction of insulin, an investigation was made into the extraction of insulin from isolated rat liver perfused with portal venous effluent (PVE) obtained from the isolated perfused rat intestine. Rat intestine was perfused with Krebs-Ringer bicarbonate medium for 45 min, and the PVE was collected from glucose-, lipid-, or NaCl-treated and untreated control intestines. The PVE, after adjustment of its glucose (180 mg/dl) and insulin (200 microU/ml) concentrations, was used as the perfusing medium for the liver of a different rat. The liver was perfused without recirculation with the PVE not containing insulin for 15 min and then perfused with the PVE containing insulin for the next 30 min. Insulin removal from liver perfused with PVE from lipid- or NaCl-treated intestine (52.6 +/- 5.4 or 46.6 +/- 4.1%) was similar to that from comparable controls (49.7 +/- 2.8 or 48.2 +/- 2.9%), respectively. However, that from glucose-treated intestine (39.7 +/- 6.2%) was significantly (P less than 0.01) lower than that from control intestine (51.1 +/- 2.5%). These results indicate that an intestinal factor secreted after glucose ingestion significantly reduces hepatic extraction of insulin and that at least a part of the incretin phenomenon is due to a decreased hepatic extraction of insulin after oral glucose administration.

Liver Blood Flow as a Major Determinant of the Clearance of Recombinant Human Tissue-Type Plasminogen Activator

1992 ◽  
Vol 67 (01) ◽  
pp. 083-087 ◽  
Author(s):  
A de Boer ◽  
C Kluft ◽  
J M Kroon ◽  
F J Kasper ◽  
H C Schoemaker ◽  
...  
Keyword(s):  
Blood Flow ◽  
Rat Liver ◽  
Healthy Subjects ◽  
Liver Blood Flow ◽  
Major Determinant ◽  
Tissue Type ◽  
Perfused Rat Liver ◽  
Liver Model ◽  
Type Plasminogen Activator ◽  
Proportional Change

SummaryThe influence of changes in liver blood flow on the clearance of rt-PA was studied both in healthy subjects and in a perfused rat liver model. Liver blood flow in healthy subjects was documented indirectly by the clearance of indocyanine green (ICG). Exercise reduced liver blood flow on average by 57% with a 95% confidence interval (95% Cl) ranging from 51% to 62% (n = 5) and increased plasma levels of rt-PA activity (after an i. v. infusion of 18 mg of rt-PA over 120 min) by 119% (95% Cl, 58% - 203%) and rt-PA antigen by 91% (95% Cl, 30% - 140%). In the perfused rat liver model it was shown that halving or doubling of the physiological flow rate of a perfusate, containing rt-PA caused a proportional change in the clearance of rt-PA, while the extraction of rt-PA by the liver remained similar. In conclusion, liver blood flow is a major determinant of the clearance of rt-PA. This may have important implications for dosage of rt-PA in patients with myocardial infarction.

A Comparison of Plasminogen Activators Derived from Rat Plasma, Primary Rat Hepatocytes and Isolated Perfused Rat Liver

1982 ◽  
Vol 47 (02) ◽  
pp. 166-172 ◽  
Author(s):  
Yoav Sharoni ◽  
Maria C Topal ◽  
Patricia R Tuttle ◽  
Henry Berger
Keyword(s):  
Rat Liver ◽  
Isoelectric Point ◽  
Rat Hepatocytes ◽  
Cell Types ◽  
Plasminogen Activators ◽  
Rat Plasma ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Molecular Weights ◽  
Physiological Relevance

SummaryOf the two cell types it was possible to culture from the dissociated rat liver, hepatocytes and Kupffer cells, only the former were fibrinolytically active. Rat hepatocytes during the first 24 hr in culture secreted two plasminogen activators with molecular weights identical to those found in rat plasma, an 80,000-dalton form (PA-80) and a 45,000-dalton form (PA-45). Partially purified preparations of plasminogen activators from both sources were subjected to isoelectric focusing (IEF) to compare characteristics further. There were three distinct peaks of PA-45 in each preparation with isoelectric points of 7.1, 7.2 and 7.4; all electrophoretic forms had the same low affinity to fibrin. PA-80 from both sources displayed similar IEF profiles with forms ranging from pH values of 7 to 8, all with the same high affinity to fibrin. The major form of PA-80 in the plasma preparation had an isoelectric point of 7.9 whereas that in the hepatocyte preparation had an isoelectric point of 7.6. The isolated perfused rat liver was also shown to produce both PA-80 and PA-45 emphasizing the physiological relevance of the findings with hepatocytes. It is concluded that in the rat hepatocytes contribute to the plasma profile with regard to the plasminogen activator content.

Troxerutin protects the isolated perfused rat liver from a possible lipid peroxidation by coumarin

Phytomedicine ◽  
2005 ◽  
Vol 12 (1-2) ◽  
pp. 52-61 ◽  
Author(s):  
B.S. Adam ◽  
R. Pentz ◽  
C.P. Siegers ◽  
O. Strubelt ◽  
M. Tegtmeier
Keyword(s):  
Lipid Peroxidation ◽  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver
Sign in / Sign up

Export Citation Format

Share Document