Kinetic characterization, thermo-stability and Reactive Red 195A dye detoxifying properties of manganese peroxidase-coupled gelatin hydrogel

2016 ◽  
Vol 74 (8) ◽  
pp. 1809-1820 ◽  
Author(s):  
Muhammad Bilal ◽  
Muhammad Asgher ◽  
Hongbo Hu ◽  
Xuehong Zhang
Keyword(s):  
Manganese Peroxidase ◽  
Oxygen Demand ◽  
Growth Conditions ◽  
Quality Parameters ◽  
Maximum Activity ◽  
White Rot ◽  
Original Activity ◽  
Gelatin Matrix ◽  
Apparent Homogeneity ◽  
Thermo Stability

An indigenous and industrially important manganese peroxidase (MnP) was isolated from solid-state bio-processing of wheat bran by white-rot fungal strain Ganoderma lucidum IBL-05 under pre-optimized growth conditions. Crude MnP extract was partially purified (2.34-fold) to apparent homogeneity by ammonium sulphate precipitation and dialysis. The homogeneous enzyme preparation was encapsulated on gelatin matrix using glutaraldehyde as a cross-linking agent. Optimal conditions for highest immobilization (82.5%) were: gelatin 20% (w/v), glutaraldehyde 0.25% (v/v) and 2 h activation time using 0.6 mg/mL of protein concentration. Gelatin-encapsulated MnP presented its maximum activity at pH 6.0 and 60 °C. Thermo-stability was considerably improved after immobilization. The optimally active MnP fraction was tested against MnSO4 as a substrate to calculate kinetic parameters. More than 90% decolorization of Sandal-fix Red C4BLN (Reactive Red 195A) dye was achieved with immobilized MnP in 5 h. It also preserved more than 50% of its original activity after the sixth reusability cycle. The water quality parameters (pH, chemical oxygen demand, total organic carbon) and cytotoxicity (brine shrimp and Daphnia magna) studies revealed the non-toxic nature of the bio-treated dye sample. A lower Km, higher Vmax, greater acidic and thermal-resistant up to 60 °C were the improved catalytic features of immobilized MnP suggesting its suitability for a variety of biotechnological applications.

Effect of growth conditions on the production of manganese peroxidase by three strains of Bjerkandera adusta

2001 ◽  
Vol 47 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Yuxin Wang ◽  
Rafael Vazquez-Duhalt ◽  
Michael A Pickard
Keyword(s):  
Rice Bran ◽  
Manganese Peroxidase ◽  
Enzyme Production ◽  
Scale Up ◽  
Growth Conditions ◽  
White Rot ◽  
Stirred Tank ◽  
Bjerkandera Adusta ◽  
Stirred Tank Reactors ◽  
Cereal Bran

We were looking for a strain of Bjerkandera adusta that produces high titres of manganese peroxidase under optimal conditions for large-scale enzyme purification. We have chosen two strains from the University of Alberta Microfungus Collection and Herbarium, UAMH 7308 and 8258, and compared the effects of growth conditions and medium composition on enzyme production with the well-characterized strain BOS55 (ATCC 90940). Of four types of cereal bran examined, rice bran at 3% (w/v) in 60 mM phosphate buffer pH 6 supported the highest levels of enzyme production. Using 100 mL medium in 500-mL Erlenmeyer flasks, maximum enzyme levels in the culture supernatant occurred after about 10 days of growth; 5.5 U·mL–1 for UAMH 7308, 4.4 U·mL–1 for UAMH 8258, and 1.7 U·mL–1 for BOS55, where units are expressed as micromoles of Mn-malonate formed per minute. Growth as submerged cultures in 10-L stirred tank reactors produced 3.5 U·mL–1 of manganese peroxidase (MnP) by UAMH 8258 and 2.5 U·mL–1 of MnP by 7308, while enzyme production by BOS55 was not successful in stirred tank reactors but could be scaled up in 2-L shake flasks containing 400 mL rice bran or glucose – malt – yeast extract (GMY) – Mn-glycolate medium to produce MnP levels of 1.7 U·mL–1. These results show that the two strains of B. adusta, UAMH 7308 and 8258, can produce between two and three times the manganese peroxidase level of B. adusta BOS55, that they are good candidates for scale up of enzyme production, and that the rice bran medium supports higher levels of enzyme production than most previously described media.Key words: growth conditions, cereal bran, manganese peroxidase, Bjerkandera adusta, white rot fungi.

scholarly journals Lignin-degrading activity and ligninolytic enzymes of different white-rot fungi: effects of manganese and malonate

1997 ◽  
Vol 75 (1) ◽  
pp. 61-71 ◽  
Author(s):  
Tamara Vares ◽  
Annele Hatakka
Keyword(s):  
Lignin Peroxidase ◽  
Manganese Peroxidase ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Growth Conditions ◽  
Fungal Species ◽  
White Rot ◽  
Trametes Hirsuta ◽  
Air Atmosphere ◽  
Trametes Trogii

Ten species of white-rot fungi, mainly belonging to the family Polyporaceae (Basidiomycotina), were studied in terms of their ability to degrade14C-ring labelled synthetic lignin and secrete ligninolytic enzymes in liquid cultures under varying growth conditions. Lignin mineralization by the fungi in an air atmosphere did not exceed 14% within 29 days. Different responses to the elevated Mn2+concentration and the addition of a manganese chelator (sodium malonate) were observed among various fungal species. This could be related with the utilization of either lignin peroxidase (LiP) or manganese peroxidase (MnP) for lignin depolymerization, i.e., some fungi apparently had an LiP-dominating ligninolytic system and others an MnP-dominating ligninolytic system. The LiP isoforms were purified from Trametes gibbosa and Trametes trogii. Isoelectric focusing of purified ligninolytic enzymes revealed the expression of numerous MnP isoforms in Trametes gibbosa, Trametes hirsuta, Trametes trogii, and Abortiporus biennis grown under a high (50-fold) Mn2+level (120 μM) with the addition of the chelator. In addition, two to three laccase isoforms were detected. Key words: white-rot fungi, lignin degradation, lignin peroxidase, manganese peroxidase, manganese, malonate.

The Chao Phraya River Basin: water quality and anthropogenic influences

2018 ◽  
Vol 19 (5) ◽  
pp. 1287-1294 ◽  
Author(s):  
Nuanchan Singkran ◽  
Pitchaya Anantawong ◽  
Naree Intharawichian ◽  
Karika Kunta
Keyword(s):  
Water Quality ◽  
Land Use ◽  
River Basin ◽  
River Mouth ◽  
Oxygen Demand ◽  
Quality Parameters ◽  
Paddy Fields ◽  
Coliform Bacteria ◽  
Wet Season ◽  
Chao Phraya River

Abstract Land use influences and trends in water quality parameters were determined for the Chao Phraya River, Thailand. Dissolved oxygen (DO), biochemical oxygen demand (BOD), and nitrate-nitrogen (NO3-N) showed significant trends (R2 ≥ 0.5) across the year, while total phosphorus (TP) and faecal coliform bacteria (FCB) showed significant trends only in the wet season. DO increased, but BOD, NO3-N, and TP decreased, from the lower section (river kilometres (rkm) 7–58 from the river mouth) through the middle section (rkm 58–143) to the upper section (rkm 143–379) of the river. Lead and mercury showed weak/no trends (R2 < 0.5). Based on the river section, major land use groups were a combination of urban and built-up areas (43%) and aquaculture (21%) in the lower river basin, paddy fields (56%) and urban and built-up areas (21%) in the middle river basin, and paddy fields (44%) and other agricultural areas (34%) in the upper river basin. Most water quality and land use attributes had significantly positive or negative correlations (at P ≤ 0.05) among each other. The river was in crisis because of high FCB concentrations. Serious measures are suggested to manage FCB and relevant human activities in the river basin.

scholarly journals Cladoceran abundance in relation to water quality parameters of Chakki Talab, Bodhan, Telangana

2018 ◽  
Vol 8 (01) ◽  
Author(s):  
Vasudha Lingampally ◽  
V.R. Solanki ◽  
D. L. Anuradha ◽  
Sabita Raja
Keyword(s):  
Water Quality ◽  
Dissolved Oxygen ◽  
Oxygen Demand ◽  
Total Dissolved Solids ◽  
Quality Parameters ◽  
Biological Oxygen Demand ◽  
Water Quality Parameters ◽  
Total Alkalinity ◽  
Total Hardness ◽  
One Year

In the present study an attempt has been made to evaluate water quality and related density of Cladocerans for a period of one year, October 2015 to September 2016. Water quality parameters such as temperature, PH, total dissolved solids, dissolved oxygen, biological oxygen demand, total alkalinity, total hardness, chlorides, phosphates, and nitrates are presented here to relate with the abundance of Cladocerans. The Cladoceran abundance reflects the eutrophic nature of the Chakki talab.

scholarly journals Treatment of industrial mineral oil wastewater – effects of coagulant type and dosage

2017 ◽  
Vol 12 (1) ◽  
pp. 139-145 ◽  
Author(s):  
E. Kweinor Tetteh ◽  
S. Rathilal ◽  
K. Robinson
Keyword(s):  
Oxygen Demand ◽  
Mineral Oil ◽  
Quality Parameters ◽  
Oil Refinery ◽  
Oil And Grease ◽  
Water And Wastewater Treatment ◽  
Dissolved Air Flotation ◽  
Jar Test ◽  
Industrial Mineral ◽  
Oil Wastewater

The use of coagulants is essential in the diverse disciplines of conventional water and wastewater treatment. This work aimed to select an economic and effective coagulant, to minimize the cost of treatment and the oil droplet content of the water, thus enhancing the efficiency of a local South African oil refinery effluent plant recovering water and oil for reuse by treating the industrial mineral oil wastewater. A standard dissolved air flotation jar test preceded evaluation of four coagulants, viz. aluminum sulfate (Alum), aluminum chloride, ferric sulfate and ferric chloride. Chemical oxygen demand, soap oil and grease, total suspended solids and turbidity were determined as water quality parameters to check coagulant efficiency. Removal of over 70% was achieved for each parameter. The results obtained at pH 5 and coagulant dose of 50 mg/L showed that alum was the best pretreatment coagulant for destabilizing and minimizing oil droplets in water, due to its trivalent cationic nature. It was also economically viable.

Depolymerization of Straw Lignin by Manganese Peroxidase from Nematoloma frowardii is Accompanied by Release of Carbon Dioxide

Holzforschung ◽  
1999 ◽  
Vol 53 (2) ◽  
pp. 161-166 ◽  
Author(s):  
Martin Hofrichter ◽  
Katrin Scheibner ◽  
Friedemann Bublitz ◽  
Ivonne Schneegaß ◽  
Dirk Ziegenhagen ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Manganese Peroxidase ◽  
Gel Permeation Chromatography ◽  
Water Soluble ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Fraction ◽  
Molecular Masses ◽  
Total Lignin ◽  
Soluble Part

SummaryManganese peroxidase preparations (MnP) from the white-rot fungusNematoloma frowardiiwere able to release14CO2directly from14C-labeled milled wheat straw (MWS; total lignin fraction) and milled straw lignin (MSL; dioxane soluble part of MWS). Apart from the formation of14CO2(4–10 %) the treatment of insoluble MWS and MSL with MnP resulted in the formation of water-soluble14C-lignin fragments (lignin solubilization, 14–25%). Analyses with gel permeation chromatography (GPC) demonstrated the formation of lignin fragments with predominant molecular masses around 1 kDa. The extent of MWS mineralization and solubilization was enhanced in the presence of reduced glutathione (GSH) acting as thiol mediator, whereas MSL mineralization was not stimulated by GSH. The principle of direct extracellular mineralization of lignin catalyzed by the MnP system may make a significant contribution to the formation of carbon dioxide in lignincellulose containing habitats.

Ecological assessment of water quality for different water uses: the upstream sub-basin of the Dnieper and Desna rivers

2021 ◽  
Vol 12 (2) ◽  
Author(s):  
V Strokal ◽  
◽  
A Kovpak ◽  
Keyword(s):  
Water Pollution ◽  
Water Quality ◽  
Oxygen Demand ◽  
Agricultural Runoff ◽  
Ammonium Nitrogen ◽  
Quality Parameters ◽  
Harmful Algae ◽  
Main Research ◽  
Average Temperature ◽  
The Status

Novelties of this study include a synthesis of water quality parameters for the upstream sub-basin of the Dnieper River. This upstream sub-basin includes the Desna River. The synthesis revels new insights on the sources of the water pollution and the status of the water quality for different purposes such as drinking, aquaculture and recreation. The main research objective was to identify the main sources of water pollution and how those sources could decrease the water quality. As a result of our analysis, we conclude the following. The levels of ammonium-nitrogen and nitrite-nitrogen in the Desna River (upstream sub-basin) are by 2-43 times and up to 53 times higher than the water quality thresholds, respectively. This poses a risk for recreational activities since too much nutrients often lead to blooms of harmful algae. We also find an increased level of biological oxygen demand in the river for drinking purposes. For aquaculture, decreased levels of dissolved oxygen are found. Climate change has an impact on water quality. For example, extreme floods caused by too much precipitation can bring pollutants to nearby waters. Monthly average temperature has increased by +2.7 degrees contributing to increased microbiological processes that could stimulate blooms of harmful algae. Main sources of water pollution are sewage discharges in cities, agricultural runoff and erosion activities after floods.

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