Treatment of corn ethanol distillery wastewater using two-stage anaerobic digestion

2016 ◽  
Vol 74 (2) ◽  
pp. 431-437 ◽  
Author(s):  
B. Ráduly ◽  
L. Gyenge ◽  
Sz. Szilveszter ◽  
A. Kedves ◽  
S. Crognale
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Oxygen Demand ◽  
Continuous Phase ◽  
Methane Content ◽  
Microbial Consortia ◽  
Two Stage ◽  
Second Stage ◽  
Conversion Rates ◽  
Distillery Wastewater

In this study the mesophilic two-stage anaerobic digestion (AD) of corn bioethanol distillery wastewater is investigated in laboratory-scale reactors. Two-stage AD technology separates the different sub-processes of the AD in two distinct reactors, enabling the use of optimal conditions for the different microbial consortia involved in the different process phases, and thus allowing for higher applicable organic loading rates (OLRs), shorter hydraulic retention times (HRTs) and better conversion rates of the organic matter, as well as higher methane content of the produced biogas. In our experiments the reactors have been operated in semi-continuous phase-separated mode. A specific methane production of 1,092 mL/(L·d) has been reached at an OLR of 6.5 g TCOD/(L·d) (TCOD: total chemical oxygen demand) and a total HRT of 21 days (5.7 days in the first-stage, and 15.3 days in the second-stage reactor). Nonetheless the methane concentration in the second-stage reactor was very high (78.9%); the two-stage AD outperformed the reference single-stage AD (conducted at the same reactor loading rate and retention time) by only a small margin in terms of volumetric methane production rate. This makes questionable whether the higher methane content of the biogas counterbalances the added complexity of the two-stage digestion.

scholarly journals Use of biogas biscuit meal EKPO-EB for agricultural biogas plant for substitution of energy crops utilization with organic waste

2015 ◽  
Vol 17 (3) ◽  
pp. 40-46
Author(s):  
Kateřina Chamrádová ◽  
Jiří Rusín
Keyword(s):  
Anaerobic Digestion ◽  
Laboratory Experiment ◽  
Methane Production ◽  
Organic Waste ◽  
Dry Mass ◽  
Corn Silage ◽  
Biogas Plant ◽  
High Yield ◽  
Two Stage ◽  
Second Stage

Abstract A laboratory experiment of two-stage mesophilic, low-dry mass, anaerobic digestion was carried out, focused on verifying the benefit of processing the biscuit meal EKPO-EB instead of triticale silage Agostino (GPS) and corn silage LG3266 in a regular batch for the agricultural biogas station in Pustějov. While anaerobic digestion of ensilages is largely difficult due to the content of lignocellulose, biscuit meal provides a high yield of biogas or methane, respectively, thanks to its high content of simple saccharides and lipids. When the original GPS (or the replacement EKPO-EB, respectively) represented 0.81% of weight of the daily input mixture dose for the first stage, the rise in volumetric methane production was 20% which is significant. The biscuit meal EKPO-EB decomposes almost completely in the first stage. Later, when the EKPO-EB represented 1.63% of weight of the daily input mixture dose for the first stage, the rise in volumetric methane production was 54% in the first stage and 16% in the second stage.

scholarly journals Evaluation of Anaerobic Digestion of Dairy Wastewater in an Innovative Multi-Section Horizontal Flow Reactor

Energies ◽  
2020 ◽  
Vol 13 (9) ◽  
pp. 2392 ◽  
Author(s):  
Marcin Dębowski ◽  
Marcin Zieliński ◽  
Marta Kisielewska ◽  
Joanna Kazimierowicz
Keyword(s):  
Wastewater Treatment ◽  
Anaerobic Digestion ◽  
Flow Reactor ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Methane Content ◽  
Dairy Wastewater ◽  
Horizontal Flow ◽  
Organic Loading Rate ◽  
Biogas Yield

The aim of this study was the performance evaluation of anaerobic digestion of dairy wastewater in a multi-section horizontal flow reactor (HFAR) equipped with microwave and ultrasonic generators to stimulate biochemical processes. The effects of increasing organic loading rate (OLR) ranging from 1.0 g chemical oxygen demand (COD)/L·d to 4.0 g COD/L·d on treatment performance, biogas production, and percentage of methane yield were determined. The highest organic compounds removals (about 85% as COD and total organic carbon—TOC) were obtained at OLR of 1.0–2.0 g COD/L·d. The highest biogas yield of 0.33 ± 0.03 L/g COD removed and methane content in biogas of 68.1 ± 5.8% were recorded at OLR of 1.0 g COD/L·d, while at OLR of 2.0 g COD/L·d it was 0.31 ± 0.02 L/COD removed and 66.3 ± 5.7%, respectively. Increasing of the OLR led to a reduction in biogas productivity as well as a decrease in methane content in biogas. The best technological effects were recorded in series with an operating mode of ultrasonic generators of 2 min work/28 min break. More intensive sonication reduced the efficiency of anaerobic digestion of dairy wastewater as well as biogas production. A low nutrient removal efficiency was observed in all tested series of the experiment, which ranged from 2.04 ± 0.38 to 4.59 ± 0.68% for phosphorus and from 9.67 ± 3.36 to 20.36 ± 0.32% for nitrogen. The effects obtained in the study (referring to the efficiency of wastewater treatment, biogas production, as well as to the results of economic analysis) proved that the HFAR can be competitive to existing industrial technologies for food wastewater treatment.

Application of Methanobrevibacter acididurans in anaerobic digestion

2004 ◽  
Vol 50 (6) ◽  
pp. 109-114 ◽  
Author(s):  
D.V. Savant ◽  
D.R. Ranade
Keyword(s):  
Fatty Acids ◽  
Anaerobic Digestion ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Low Ph ◽  
Anaerobic Digesters ◽  
Hydrogenotrophic Methanogen ◽  
Distillery Wastewater ◽  
Acid Tolerant ◽  
Acidic Conditions

To operate anaerobic digesters successfully under acidic conditions, hydrogen utilizing methanogens which can grow efficiently at low pH and tolerate high volatile fatty acids (VFA) are desirable. An acid tolerant hydrogenotrophic methanogen viz. Methanobrevibacter acididurans isolated from slurry of an anaerobic digester running on alcohol distillery wastewater has been described earlier by this lab. This organism could grow optimally at pH 6.0. In the experiments reported herein, M. acididurans showed better methanogenesis under acidic conditions with high VFA, particularly acetate, than Methanobacterium bryantii, a common hydrogenotrophic inhabitant of anaerobic digesters. Addition of M. acididurans culture to digesting slurry of acidogenic as well as methanogenic digesters running on distillery wastewater showed increase in methane production and decrease in accumulation of volatile fatty acids. The results proved the feasibility of application of M. acididurans in anaerobic digesters.

scholarly journals Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion

2019 ◽  
Vol 13 (1) ◽  
pp. 260-270 ◽  
Author(s):  
Jonas A. Ohlsson ◽  
Anne E. Harman-Ware ◽  
Mats Sandgren ◽  
Anna Schnürer
Keyword(s):  
Anaerobic Digestion ◽  
Enzymatic Hydrolysis ◽  
Methane Production ◽  
Early Stage ◽  
Large Population ◽  
Spectral Features ◽  
Biomass Recalcitrance ◽  
Conversion Rates ◽  
Biological Conversion ◽  
Conversion Performance

AbstractBiomass recalcitrance, the inherent resistance of plants towards deconstruction, negatively affects the viability of biorefineries. This trait is not only dictated by the properties of the biomass but also by the conversion system used and its interactions with specific features of the biomass. Here, biomass recalcitrance to anaerobic digestion (AD) was assessed using a biomethanation potential (BMP) assay. Plant material (n = 94) was selected from a large population of natural Salix viminalis accessions, previously evaluated for biomass recalcitrance using hydrothermal pretreatment–enzymatic hydrolysis. Correlations between yields from the two biological conversion systems were evaluated, as well as the influence of biomass compositional features, analyzed by pyrolysis-molecular beam mass spectrometry (py-MBMS), and other biomass physical properties on conversion performance. BMP values averaged 198.0 Nml CH4/g biomass after 94 days, ranging from 28.6 to 245.9. S lignin and carbohydrate-derived spectral features were positively correlated with performance under both systems, whereas G lignin, p-coumaric acid, and ferulic acid-derived ions were negatively correlated with yields and rates. Most spectral features were more strongly correlated with enzymatic hydrolysis yields compared to methane production. For early-stage methane production and rate, recalcitrance factors were similar compared to enzymatic hydrolysis, with weaker correlations observed at later timepoints. The results suggest that although variation in methane potential was considerably lower than enzymatic hydrolysis yields, a reduced recalcitrance under this system will still be of importance to improve early conversion rates. Spectral features of low methane-producing samples indicate the presence of inhibitory substances, warranting further study.

scholarly journals A Review on Anaerobic Co-Digestion with a Focus on the Microbial Populations and the Effect of Multi-Stage Digester Configuration

Energies ◽  
2019 ◽  
Vol 12 (6) ◽  
pp. 1106 ◽  
Author(s):  
Anahita Rabii ◽  
Saad Aldin ◽  
Yaser Dahman ◽  
Elsayed Elbeshbishy
Keyword(s):  
Anaerobic Digestion ◽  
Microbial Population ◽  
System Stability ◽  
Microbial Consortia ◽  
Microbial Populations ◽  
Stage Versus ◽  
Two Stage ◽  
Waste Sludge ◽  
Multi Stage ◽  
Mode A

Recent studies have shown that anaerobic co-digestion (AnCoD) is superior to conventional anaerobic digestion (AD). The benefits of enhanced bioenergy production and solids reduction using co-substrates have attracted researchers to study the co-digestion technology and to better understand the effect of multi substrates on digester performance. This review will discuss the results of such studies with the main focus on: (1) generally the advantages of co-digestion over mono-digestion in terms of system stability, bioenergy, and solids reduction; (2) microbial consortia diversity and their synergistic impact on biogas improvement; (3) the effect of digester mode, i.e., multi-stage versus single stage digestion on AnCoD. It is essential to note that the studies reported improvement in the synergy and diverse microbial consortia when using co-digestion technologies, in addition to higher biomethane yield when using two-stage mode. A good example would be the co-digestion of biodiesel waste and glycerin with municipal waste sludge in a two-stage reactor resulting in 100% increase of biogas and 120% increase in the methane content of the produced biogas with microbial population dominated by Methanosaeta and Methanomicrobium.

Hydrogen and methane production from vinasse using two-stage anaerobic digestion

2017 ◽  
Vol 107 ◽  
pp. 81-86 ◽  
Author(s):  
Shan-Fei Fu ◽  
Xiao-Hui Xu ◽  
Meng Dai ◽  
Xian-Zheng Yuan ◽  
Rong-Bo Guo
Keyword(s):  
Anaerobic Digestion ◽  
Methane Production ◽  
Two Stage

Temperature effects on the trophic stages of perennial rye grass anaerobic digestion

2011 ◽  
Vol 64 (1) ◽  
pp. 70-76 ◽  
Author(s):  
D. Cysneiros ◽  
A. Thuillier ◽  
R. Villemont ◽  
A. Littlestone ◽  
T. Mahony ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Low Temperature ◽  
Methane Production ◽  
Volatile Fatty Acids ◽  
Oxygen Demand ◽  
Production Performance ◽  
Batch Mode ◽  
Rye Grass ◽  
Long Term Experiment ◽  
Rate Limiting

Continuous Stirred Tank Reactors (CSTRs), operated in batch mode, were used to evaluate the feasibility of psychrophilic (low temperature) digestion of perennial rye grass in a long term experiment (150 days) for the first time. The reactors were operated in parallel at 3 different temperatures, 10, 15 and 37 °C. Hydrolysis, acidification and methanogenesis were assessed by VS degradation, by soluble chemical oxygen demand (SCOD) and volatile fatty acids (VFA) production, and by methane production, respectively. Hydrolysis was the rate-limiting step at all temperatures and the rates and extent of hydrolysis were considerably lower at 15 and 10 °C, than at 37 °C. The total VS degradation was 53%, 34% and 19% at 37, 15 and 10 °C, respectively. Acidification was not affected by temperature and VFA production and consumption was balanced in all cases, except at 10 °C. Methane yields were 0.215 m3 CH4 kg−1 VS−1 added, 0.160 m3 CH4 kg−1 VS−1 added and 0.125 m3 CH4 kg−1 VS−1 added at 37, 15 and 10 °C, respectively. Methanogenesis was not strongly affected at 15 °C but it became rate-limiting at 10 °C. Overall, the solid degradation and methane production performance under psychrophilic conditions was encouraging and greater than previously reported. Considering the non-acclimated, mesophilic nature of the inoculum, there are grounds to believe that low-temperature anaerobic digestion of grass could be feasible if coupled to efficient hydrolysis of the biomass.

Bio-hydrogen and bio-methane production from food waste in a two-stage anaerobic digestion process with digestate recirculation

2019 ◽  
Vol 130 ◽  
pp. 1108-1115 ◽  
Author(s):  
Dalal E. Algapani ◽  
Wei Qiao ◽  
Marina Ricci ◽  
Davide Bianchi ◽  
Simon M. Wandera ◽  
...  
Keyword(s):  
Anaerobic Digestion ◽  
Food Waste ◽  
Methane Production ◽  
Two Stage ◽  
Anaerobic Digestion Process ◽  
Digestion Process

scholarly journals Enhancement anaerobic digestion and methane production from kitchen waste by thermal and thermo-chemical pretreatments in batch  leach bed reactor with down flow

2018 ◽  
Vol 64 (No. 3) ◽  
pp. 128-135 ◽  
Author(s):  
Radmard Seyed Abbas ◽  
Alizadeh Hossein Haji Agha ◽  
Seifi Rahman
Keyword(s):  
Anaerobic Digestion ◽  
Microwave Irradiation ◽  
Methane Production ◽  
Biogas Production ◽  
Oxygen Demand ◽  
Lag Phase ◽  
Kitchen Waste ◽  
Gompertz Equation ◽  
Chemical Pretreatments ◽  
Pretreatment Conditions

The effects of thermal (autoclave and microwave irradiation (MW)) and thermo-chemical (autoclave and microwave irradiation – assisted NaOH 5N) pretreatments on the chemical oxygen demand (COD) solubilisation, biogas and methane production of anaerobic digestion kitchen waste (KW) were investigated in this study. The modified Gompertz equation was fitted to accurately assess and compare the biogas and methane production from KW under the different pretreatment conditions and to attain representative simulations and predictions. In present study, COD solubilisation was demonstrated as an effective effect of pretreatment. Thermo-chemical pretreatments could improve biogas and methane production yields from KW. A comprehensive evaluation indicated that the thermo-chemical pretreatments (microwave irradiation and autoclave- assisted NaOH 5N, respectively) provided the best conditions to increase biogas and methane production from KW. The most effective enhancement of biogas and methane production (68.37 and 36.92 l, respectively) was observed from MW pretreated KW along with NaOH 5N, with the shortest lag phase of 1.79  day, the max. rate of 2.38 l·day<sup>–1</sup> and ultimate biogas production of 69.8 l as the modified Gompertz equation predicted.

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