The kinetics for ammonium and nitrite oxidation under the effect of hydroxylamine

2015 ◽  
Vol 73 (5) ◽  
pp. 1067-1073 ◽  
Author(s):  
Xinyu Wan ◽  
Pengying Xiao ◽  
Daijun Zhang ◽  
Peili Lu ◽  
Zongbao Yao ◽  
...  
Keyword(s):  
Sequencing Batch Reactor ◽  
Ammonia Oxidation ◽  
Oxygen Uptake Rate ◽  
Batch Reactor ◽  
Ammonia Oxidizing Bacteria ◽  
Nitrite Oxidation ◽  
Kinetics Parameters ◽  
Nitrification Process ◽  
Nitrite Oxidizing Bacteria ◽  
No2 Oxidation

The kinetics for ammonium (NH4+) oxidation and nitrite (NO2−) oxidation under the effect of hydroxylamine (NH2OH) were studied by respirometry using the nitrifying sludge from a laboratory-scale sequencing batch reactor. Modified models were used to estimate kinetics parameters of ammonia and nitrite oxidation under the effect of hydroxylamine. An inhibition effect of hydroxylamine on the ammonia oxidation was observed under different hydroxylamine concentration levels. The self-inhibition coefficient of hydroxylamine oxidation and noncompetitive inhibition coefficient of hydroxylamine for nitrite oxidation was estimated by simulating exogenous oxygen-uptake rate profiles, respectively. The inhibitive effect of NH2OH on nitrite-oxidizing bacteria was stronger than on ammonia-oxidizing bacteria. This work could provide fundamental data for the kinetic investigation of the nitrification process.

Inhibition of Nitrification of Ammonia-Rich Wastewater in Immobilized Nitrifiers System

2011 ◽  
Vol 183-185 ◽  
pp. 197-200 ◽  
Author(s):  
Zhi Rong Li ◽  
Zhi Zhang ◽  
Zhen Jia Zhang
Keyword(s):  
Ammonia Oxidation ◽  
High Strength ◽  
Ammonia Solution ◽  
Ammonia Oxidizing Bacteria ◽  
Inhibition Kinetics ◽  
Nitrite Oxidation ◽  
Haldane Model ◽  
Nitrite Oxidizing Bacteria ◽  
Inhibition Of Nitrification ◽  
Kinetics Of

Inhibition of ammonia oxidation and nitrite oxidation was studied in an immobilized biomass system. Ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) suffered different inhibition in ammonia-rich wastewater. NOB started to be inhibited when FA (free ammonia) was 2mg/L, and was totally inhibited when FA was 8mg/L. AOB started to be inhibited when FA was 22mg/L, and tended to lose activity when FA was higher than 170mg/L. Inhibition kinetics of ammonia oxidation and nitrite oxidation could be described by Haldane model. Immobilization alleviated inhibition of FA to nitrifiers, maintaining high activity even at high strength ammonia solution. Partial nitrification could be achieved by varying degrees of inhibition of FA to AOB and NOB.

Post-treatment of a slaughterhouse wastewater: stability of the microbial community of a sequencing batch reactor operated under oxygen limited conditions

2006 ◽  
Vol 54 (2) ◽  
pp. 215-221 ◽  
Author(s):  
A. Cabezas ◽  
P. Draper ◽  
L. Muxí ◽  
C. Etchebehere
Keyword(s):  
Microbial Community ◽  
Organic Nitrogen ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Anaerobic Treatment ◽  
Post Treatment ◽  
Ammonia Oxidizing Bacteria ◽  
Slaughterhouse Wastewater ◽  
Nitrite Oxidizing Bacteria ◽  
The Stability

Slaughterhouse wastewater is a complex effluent with an important content of organic nitrogen. After an anaerobic treatment where most of the organic matter is removed, the nitrogen, remains as ammonium and post-treatment of the effluent is necessary. Sequencing batch reactor (SBR) technology has been developed to completely remove nitrogen in one single reactor combining aerobic and anoxic stages. Under oxygen limited conditions only nitrite is produced with concomitant energy saving. The stability and diversity of the microbial community from a nitrifying denitrifying SBR operated under oxygen limited conditions were studied using molecular and respirometric methods. The AOB (ammonia oxidizing bacteria) community was relatively stable Nitrosomonas being the dominant genera although Nitrosospira and Nitrosococcus were detected in low proportions. Nitrite oxidizing bacteria were out competed during the operation under oxygen-limited conditions. After an increase of the DO in the reactor Nitrobacter spp were detected suggesting that they remained in the system. Changes in the AOB and denitrifying communities were observed after the DO increase. Sedimentation problems were detected during operation, this could be related to the predominance of Thauera spp detected by FISH and T-RFLP.

Settleability and kinetics of a nitrifying sludge in a sequencing batch reactor

2004 ◽  
Vol 50 (11) ◽  
pp. 943-949 ◽  
Author(s):  
A -C Texier ◽  
J Gomez
Keyword(s):  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Operation Time ◽  
Volume Index ◽  
Specific Rate ◽  
Ammonia Oxidizing Bacteria ◽  
Microbial Protein ◽  
N Accumulation ◽  
Reactor Kinetics ◽  
Nitrite Oxidizing Bacteria

A physiological study of a nitrifying sludge was carried out in a sequencing batch reactor (SBR). Pseudo steady-state nitrification conditions were obtained with an ammonium removal efficiency of 99% ± 1% and 98% ± 2% conversion of NH4+-N to NO3–-N. The rate of biomass production was negligible (1.3 ± 0.1 mg microbial protein-N·L–1·d–1). The sludge presented good settling properties with sludge volume index values lower than 20 mL·g–1 and an exopolymeric protein/carbohydrate ratio of 0.53 ± 0.34. Kinetic results indicated that the nitrifying behavior of the sludge changed with the number of cycles. After 22 cycles, a decrease in the specific rate of NO3–-N production coupled with an increase in the NO2–-N accumulation were observed. These results showed that the activity of the nitrite oxidizing bacteria decreased at a longer operation time. Ammonia oxidizing bacteria were found to exhibit the best stability. After 4 months of operation, the specific rates of NH4+-N consumption and NO3–-N production were 1.72 NH4+-N per microbial protein-N per hour (g·g–1·h–1) and 0.54 NO3–-N per microbial protein-N per hour (g·g–1·h–1), respectively.Key words: nitrification, sequencing batch reactor, kinetics, settleability, exopolymeric substances.

scholarly journals Partial nitritation treating nitrogen in old landfill leachate

2016 ◽  
Vol 19 (4) ◽  
pp. 39-49
Author(s):  
Nhat The Phan ◽  
Van Thi Thanh Truong ◽  
Son Thanh Le ◽  
Biec Nhu Ha ◽  
Dan Phuoc Nguyen
Keyword(s):  
Landfill Leachate ◽  
Retention Time ◽  
Sequencing Batch Reactor ◽  
Hydraulic Retention Time ◽  
Batch Reactor ◽  
Partial Nitritation ◽  
Time Operation ◽  
Nitrite Oxidizing Bacteria ◽  
Long Time ◽  
H 30

In this study, a lab-scale Partial Nitritation Sequencing Batch Reactor (PNSBR) was implemented for treating high-ammonium old landfill leachate to yield an appropriate NO2—N/ NH4+-N ratio from 1/1 to 1.32/1 mixture as a pretreatment for subsequent Anammox. The objective of this study was to determine the optimal hydraulic retention time (HRT) at different influent ammonia concentrations for 210 days. The experimental results showed that with the influent ammonia concentrations of 500, 1000, 1500 and 2000 mg/L, HRT is 12 h, 21 h, 30 h and 48 h, respectively. The range of free ammonia (FA) concentration from 17 to 44 mg/L completely inhibited nitrite oxidizing bacteria (NOB) for long time operation. The COD removal efficiency was very low (6±2) %.

scholarly journals Nitrification mainly driven by ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an anammox-inoculated wastewater treatment system

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Lu ◽  
Yiguo Hong ◽  
Ying Wei ◽  
Ji-Dong Gu ◽  
Jiapeng Wu ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Ammonia Oxidation ◽  
Ammonia Oxidizing Bacteria ◽  
Ammonium Oxidation ◽  
Strong Activity ◽  
Nitrite Oxidizing Bacteria

AbstractAnaerobic ammonium oxidation (anammox) process has been acknowledged as an environmentally friendly and time-saving technique capable of achieving efficient nitrogen removal. However, the community of nitrification process in anammox-inoculated wastewater treatment plants (WWTPs) has not been elucidated. In this study, ammonia oxidation (AO) and nitrite oxidation (NO) rates were analyzed with the incubation of activated sludge from Xinfeng WWTPs (Taiwan, China), and the community composition of nitrification communities were investigated by high-throughput sequencing. Results showed that both AO and NO had strong activity in the activated sludge. The average rates of AO and NO in sample A were 6.51 µmol L−1 h−1 and 6.52 µmol L−1 h−1, respectively, while the rates in sample B were 14.48 µmol L−1 h−1 and 14.59 µmol L−1 h−1, respectively. The abundance of the nitrite-oxidizing bacteria (NOB) Nitrospira was 0.89–4.95 × 1011 copies/g in both samples A and B, the abundance of ammonia-oxidizing bacteria (AOB) was 1.01–9.74 × 109 copies/g. In contrast, the abundance of ammonia-oxidizing archaea (AOA) was much lower than AOB, only with 1.28–1.53 × 105 copies/g in samples A and B. The AOA community was dominated by Nitrosotenuis, Nitrosocosmicus, and Nitrososphaera, while the AOB community mainly consisted of Nitrosomonas and Nitrosococcus. The dominant species of Nitrospira were Candidatus Nitrospira defluvii, Candidatus Nitrospira Ecomare2 and Nitrospira inopinata. In summary, the strong nitrification activity was mainly catalyzed by AOB and Nitrospira, maintaining high efficiency in nitrogen removal in the anammox-inoculated WWTPs by providing the substrates required for denitrification and anammox processes.

scholarly journals Effect of temperatures and alternating anoxic/oxic sequencing batch reactor (SBR) operating modes on extracellular polymeric substances in activated sludge

2020 ◽  
Author(s):  
Hongwei Sun ◽  
Chenjian Cai ◽  
Jixue Chen ◽  
Chunyu Liu ◽  
Guangjie Wang ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Batch Reactors ◽  
Sequencing Batch Reactors ◽  
Operating Modes ◽  
Nitrification Process ◽  
Main Component ◽  
Denitrification Process

Abstract In order to investigate the effect of temperatures and operating modes on extracellular polymeric substances (EPS) contents, three sequencing batch reactors (SBRs) were operated at temperatures of 15, 25, and 35 °C (R15 °C, R25 °C, and R35 °C, respectively), with two SBRs operated under alternating anoxic/oxic conditions (RA/O and RO/A, respectively). Results showed that higher contents of tightly bound EPS (TB-EPS) and total EPS appeared in R15 °C, while loosely bound EPS (LB-EPS) dominated in R35 °C. In all three kinds of EPS (LB-EPS, TB-EPS and total EPS) assessed, protein was the main component in R15 °C and R25 °C, while polysaccharides dominated in R35 °C. Moreover, compared with RO/A, RA/O was favorable for the production of the three kinds of EPS. Furthermore, three kinds of EPS and their components were augmented during the nitrification process, while they declined during the denitrification process under all conditions except for R35 °C.

scholarly journals Development of Strategies for AOB and NOB Competition Supported by Mathematical Modeling in Terms of Successful Deammonification Implementation for Energy-Efficient WWTPs

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 562
Author(s):  
Mehdi Sharif Shourjeh ◽  
Przemysław Kowal ◽  
Xi Lu ◽  
Li Xie ◽  
Jakub Drewnowski
Keyword(s):  
Mathematical Modeling ◽  
Ammonia Oxidation ◽  
Current Knowledge ◽  
Microbial Interactions ◽  
Process Efficiency ◽  
Ammonia Oxidizing Bacteria ◽  
Operational Conditions ◽  
Novel Technologies ◽  
Anaerobic Ammonia Oxidation ◽  
Nitrite Oxidizing Bacteria

Novel technologies such as partial nitritation (PN) and partial denitritation (PDN) could be combined with the anammox-based process in order to alleviate energy input. The former combination, also noted as deammonification, has been intensively studied in a frame of lab and full-scale wastewater treatment in order to optimize operational costs and process efficiency. For the deammonification process, key functional microbes include ammonia-oxidizing bacteria (AOB) and anaerobic ammonia oxidation bacteria (AnAOB), which coexisting and interact with heterotrophs and nitrite oxidizing bacteria (NOB). The aim of the presented review was to summarize current knowledge about deammonification process principles, related to microbial interactions responsible for the process maintenance under varying operational conditions. Particular attention was paid to the factors influencing the targeted selection of AOB/AnAOB over the NOB and application of the mathematical modeling as a powerful tool enabling accelerated process optimization and characterization. Another reviewed aspect was the potential energetic and resources savings connected with deammonification application in relation to the technologies based on the conventional nitrification/denitrification processes.

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