Rapid start-up of a nitrifying reactor using aerobic granular sludge as seed sludge

2012 ◽  
Vol 65 (3) ◽  
pp. 581-588 ◽  
Author(s):  
Naohiro Kishida ◽  
Goro Saeki ◽  
Satoshi Tsuneda ◽  
Ryuichi Sudo
Keyword(s):  
Removal Rate ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
High Rate ◽  
Aerobic Granular Sludge ◽  
Nitrite Accumulation ◽  
Ammonia Oxidizing Bacteria ◽  
Continuous Stirred Tank Reactor ◽  
Start Up ◽  
Seed Sludge

In this study, the effectiveness of aerobic granular sludge as seed sludge for rapid start-up of nitrifying processes was investigated using a laboratory-scale continuous stirred-tank reactor (CSTR) fed with completely inorganic wastewater which contained a high concentration of ammonia. Even when a large amount of granular biomass was inoculated in the reactor, and the characteristics of influent wastewater were abruptly changed, excess biomass washout was not observed, and biomass concentration was kept high at the start-up period due to high settling ability of the aerobic granular sludge. As a result, an ammonia removal rate immediately increased and reached more than 1.0 kg N/m3/d within 20 days and up to 1.8 kg N/m3/d on day 39. Subsequently, high rate nitritation was stably attained during 100 days. However, nitrite accumulation had been observed for 140 days before attaining complete nitrification to nitrate. Fluorescence in situ hybridization analysis revealed the increase in amount of ammonia-oxidizing bacteria which existed in the outer edge of the granular sludge during the start-up period. This microbial ecological change would make it possible to attain high rate ammonia removal.

High-Rate Nitrification Using Aerobic Granular Sludge

2006 ◽  
Vol 53 (3) ◽  
pp. 147-154 ◽  
Author(s):  
S. Tsuneda ◽  
M. Ogiwara ◽  
Y. Ejiri ◽  
A. Hirata
Keyword(s):  
Activated Sludge ◽  
Removal Rate ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
High Rate ◽  
Pure Oxygen ◽  
Synthetic Wastewater ◽  
Aerobic Granular Sludge ◽  
Fluidised Bed ◽  
High Concentration

The performance of nitrifying granules, which had been produced in an aerobic upflow fluidised bed (AUFB) reactor, was investigated in various types of ammonia-containing wastewaters. When pure oxygen was supplied to the AUFB reactor with a synthetic wastewater containing a high concentration of ammonia (500 g-N/m3), the ammonia removal rate reached 16.7 kg-N/m3/day with a sustained ammonia removal efficiency of more than 80%. The nitrifying granules possessing a high settling ability could be retained with a high density (approximately 10,000 g-MLSS/m3) in a continuous stirring tank reactor (CSTR) even under a short hydraulic retention time (44 min), which enabled a high-rate and stable nitrification for an inorganic wastewater containing low concentrations of ammonia (50 g-N/m3). Moreover, the nitrifying granules exhibited sufficient performance in the nitrification of real industrial wastewater containing high concentrations of ammonia (1,000–1,400 g-N/m3) and salinity (1.2–2.2%), which was discharged from metal-refinery processes. When the nitrifying granules were used in cooperation with activated sludge to treat domestic wastewater containing organic pollutants as well as ammonia, they fully contributed to nitrification even though a part of activated sludge adhered onto the granule surfaces to form biofilms. These results show the wide applicability of nitrifying granules to various cases in the nitrification step of wastewater treatment plants.

Study on Short-Cut Nitrification Aerobic Granular Sludge Cultivation and Carbon-Nitrogen Removal

2010 ◽  
Vol 113-116 ◽  
pp. 2305-2309
Author(s):  
Jing Xiang Fu ◽  
Yu Lan Tang ◽  
Xing Guan Ma ◽  
Yu Hua Zhao ◽  
Hai Biao Wang
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Batch Reactor ◽  
Granular Sludge ◽  
Average Diameter ◽  
Ammonia Concentration ◽  
Aerobic Granular Sludge ◽  
Seed Sludge ◽  
Cod Removal Rate

Acclimation of short-cut nitrification aerobic granular sludge with aerobic granular sludge and nitrification sludge as seed sludge in a sequencing batch reactor(SBR) by controlling the pH, influent ammonia concentration, temperature and other conditions. Experimental results showed that the short-cut nitrification aerobic granular sludge with high ammonia and COD removal rate (95%) was formed successfully, and SBR can run long-term stably. DO at 6.0~8.0mg/L when the nitrosation rate remained stable at 50% ~ 60%, and lower DO levels within the reactor (2.0~3.0mg/L) nitrosation rates of up to 90%. Mature short-cut nitrification aerobic granular sludge average diameter of 2~3mm in between, MLSS of up to 10.162g/L, SVI minimum up to 22.63ml/g, moisture content is only 81.93%, total nitrogen removal rate of 40%, and carbon-nitrogen removal capability.

High-rate nitrification of electronic industry wastewater by using nitrifying granules

2017 ◽  
Vol 76 (11) ◽  
pp. 3171-3180 ◽  
Author(s):  
Yoshiaki Hasebe ◽  
Hiroaki Meguro ◽  
Yuuki Kanai ◽  
Masahiro Eguchi ◽  
Toshifumi Osaka ◽  
...  
Keyword(s):  
Removal Rate ◽  
Pilot Scale ◽  
Ammonia Removal ◽  
High Rate ◽  
Synthetic Wastewater ◽  
Nitrifying Bacteria ◽  
Test Plant ◽  
Ammonia Oxidizing Bacteria ◽  
Water Conduction ◽  
Industry Wastewater

Abstract Nitrifying granules have a high sedimentation property and an ability to maintain a large amount of nitrifying bacteria in a reaction tank. Our group has examined the formation process of nitrifying granules and achieved high-rate nitrification for an inorganic synthetic wastewater using these granules. In this research, a pilot-scale test plant with an 850-liter reaction tank was assembled in a semiconductor manufacturing factory in order to conduct a continuous water conduction test using real electronics industry wastewater. The aim was to observe the formation of nitrifying granules and determine the maximum ammonia removal rate. The average granule diameter formed during the experiment was 780 μm and the maximum ammonia removal rate was observed to be 1.5 kgN·m−3·day−1 at 20 °C, which is 2.5–5 times faster than traditional activated sludge methods. A fluorescence in situ hybridization analysis showed that β-proteobacterial ammonia oxidizing bacteria and the Nitrospira-like nitrite-oxidizing bacteria dominate the bacteria population in the granules, and their strong aggregation capacity might confer some benefits to the formation of these nitrifying granules.

Rapid cultivation of aerobic granular sludge by bone glue augmentation and contaminant removal characteristics

2013 ◽  
Vol 67 (7) ◽  
pp. 1627-1633 ◽  
Author(s):  
Shuo Wang ◽  
Wenxin Shi ◽  
Shuili Yu ◽  
Xuesong Yi
Keyword(s):  
Essential Feature ◽  
Oxygen Demand ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Nitrite Accumulation ◽  
Stable Operation ◽  
Contaminant Removal ◽  
Whole Process ◽  
Start Up ◽  
Bone Glue

To achieve a quick start-up and stable operation, aerobic granular sludge (AGS) was cultivated in a sequencing batch airlift reactor (SBAR) with the addition of bone glue augmentation. Adding an amount of bone glue (40 mg L–1) can accelerate granulation, which advanced by 10 d on average. Aerobic granules of size 0.5–3.0 mm were dominant in the SBAR and the settling velocity acquired a better correlation with the size of the AGS. In addition, the content of total polysaccharides was 19.54 mg gMLSS–1 (grams of mixed liquor suspended solids) (an increase of 34.0%), the content of total protein was 60.59 mg gMLSS–1 (an increase of a factor of 33) and the total proteins/total polysaccharides ratio was 3.3. The relatively high protein content was an essential feature for cultivation of AGS, which may indicate that extracellular polymeric substance was the mechanism for granulation due to the adhesion of microorganisms by bone glue. AGS possessed better chemical oxygen demand, NH4+-N and PO43–-P removal efficiency (of 86.7, 90.6 and 93.8%, respectively) and no nitrite accumulation was observed in the whole process.

scholarly journals Rapid Start-Up of the Aerobic Granular Reactor under Low Temperature and the Nutriment Removal Performance of Granules with Different Particle Sizes

Water ◽  
2021 ◽  
Vol 13 (24) ◽  
pp. 3590
Author(s):  
Dongbo Liang ◽  
Jun Li ◽  
Zhaoming Zheng ◽  
Jing Zhang ◽  
Yaodong Wu ◽  
...  
Keyword(s):  
Low Temperature ◽  
Accumulation Rate ◽  
Removal Rate ◽  
Batch Reactor ◽  
Synthetic Wastewater ◽  
Aerobic Granular Sludge ◽  
Nitrite Accumulation ◽  
Stable Operation ◽  
N Removal ◽  
Start Up

The start-up of the aerobic granular sludge (AGS) process under low temperature is challenging. In this study, the sequencing batch reactor (SBR) was fed with synthetic wastewater and the temperature was controlled at 15 ℃. The main components in the synthetic wastewater were sodium acetate and ammonium chloride. The influent chemical oxygen demand (COD) and NH4+-N concentrations were 300 and 60 mg/L, respectively. The AGS was successfully cultivated in 60 days by gradually shortening the settling time. During the stable operation stage (61–100 d), the average effluent COD, NH4+-N, NO2−-N, and NO3−-N concentrations were 47.2, 1.0, 47.2, and 5.1 mg/L, respectively. Meanwhile, the nitrite accumulation rate (NAR) reached 90.6%. Batch test showed that the smaller AGS had higher NH4+-N removal rate while the larger AGS performed higher NAR. The NH4+-N removal rates of R1 (1.0–2.0 mm), R2 (2.0–3.0 mm), and R3 (>3 mm) granules were 0.85, 0.61, and 0.45 g N/(kg VSS·h), respectively. Meanwhile, the NAR of R1, R2, and R3 were 36.2%, 77.2%, and 94.9%, respectively. The obtained results could provide important guidance for the cultivation of AGS in low-temperature wastewater treatment.

Performance of aerobic granular sludge at variable circulation rate in anaerobic–aerobic conditions

2014 ◽  
Vol 69 (11) ◽  
pp. 2252-2257 ◽  
Author(s):  
Hasnida Harun ◽  
Aznah Nor Anuar ◽  
Zaini Ujang ◽  
Noor Hasyimah Rosman ◽  
Inawati Othman
Keyword(s):  
Municipal Wastewater ◽  
Granular Sludge ◽  
Ammonia Removal ◽  
Soy Sauce ◽  
Volume Index ◽  
Aerobic Granular Sludge ◽  
Circulation Rate ◽  
Anaerobic Conditions ◽  
Aerobic Conditions ◽  
Mixed Liquor

Aerobic granular sludge (AGS) has been applied to treat a broad range of industrial and municipal wastewater. AGS can be developed in a sequencing batch reactor (SBR) with alternating anaerobic–aerobic conditions. To provide anaerobic conditions, the mixed liquor is allowed to circulate in the reactor without air supply. The circulation flow rate of mixed liquor in anaerobic condition is the most important parameter of operation in the anaerobic-AGS processes. Therefore, this study investigates the effect of circulation rate on the performance of the SBR with AGS. Two identical reactors namely R1 and R2 were operated using fermented soy sauce wastewater at circulation rate of 14.4 and 36.0 l/h, respectively. During the anaerobic conditions, the wastewater was pumped out from the upper part of the reactor and circulated back into the bottom of the reactor for 230 min. A compact and dense AGS was observed in both reactors with a similar diameter of 2.0 mm in average, although different circulation rates were adopted. The best reactor performance was achieved in R2 with chemical oxygen demand removal rate of 89%, 90% total phosphorus removal, 79% ammonia removal, 10.1 g/l of mixed liquor suspended solids and a sludge volume index of 25 ml/g.

Effect of Shear Stress on Granulation in Oxygen Aerobic Upflow Sludge Bed Reactors

1992 ◽  
Vol 26 (3-4) ◽  
pp. 601-605 ◽  
Author(s):  
H.-S. Shin ◽  
K.-H. Lim ◽  
H.-S. Park
Keyword(s):  
Shear Stress ◽  
Morphological Study ◽  
Loading Rate ◽  
Granular Sludge ◽  
Treatment Method ◽  
The Self ◽  
High Rate ◽  
Synthetic Wastewater ◽  
Filamentous Bacteria ◽  
Start Up

Aerobic upflow sludge blanket(AUSB) process is a new biological wastewater treatment method applying the concept of the self-immobilization to activated sludge. Two sets of AUSB system with different mixing velocities of 3 rpm(R1) and 6 rpm(R2) were operated for high-rate treatment of synthetic wastewater. The COD removal efficiency in R2 was higher than R1 at the same loading rate up to 7 kg/m3·day. However, in R1, the sludge bulking was observed at the end of the experiment. The chocolate colored granules were formed about 5 days after the start-up. The morphological study on the granular sludge consortia was made with both scanning electron and optical microscopes. The granules were 0.5-2.5 mm in diameter and mainly consisted of bacteria with pili-like appendages and filamentous bacteria, which were thought to be Sphaerotilus natans and Beggiatoa. In R1, the long multicellular filaments causing bulking were prevalent in the granule, while in R2 overgrowth of filamentous bacteria was prevented with appropriate shear stress resulting in higher MLSS density. Experimental results indicated that granulation could be controlled by physical stress on granular sludge.

Start-up of an aerobic granular sequencing batch reactor for the treatment of winery wastewater

2009 ◽  
Vol 60 (4) ◽  
pp. 1049-1054 ◽  
Author(s):  
S. López–Palau ◽  
J. Dosta ◽  
J. Mata-Álvarez
Keyword(s):  
Organic Matter ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Particle Diameter ◽  
Granular Sludge ◽  
Average Density ◽  
Aerobic Granular Sludge ◽  
Organic Load ◽  
Winery Wastewater ◽  
Start Up

Aerobic granular sludge was cultivated in a sequencing batch reactor (SBR) in order to remove the organic matter present in winery wastewater. The formation of granules was performed using a synthetic substrate. The selection parameter was the settling time, as well as the alternation of feast-famine periods, the air velocity and the height/diameter ratio of the reactor. After 10 days of operation under these conditions, the first aggregates could be observed. Filamentous bacteria were still present in the reactor but they disappeared progressively. During the start-up, COD loading was increased from 2.7 to 22.5 kg COD/(m3 day) in order to obtain a feast period between 30 and 60 minutes. At this point, granules were quite round, with a particle diameter between 3.0 and 4.0 mm and an average density of 6 g L−1. After 120 days of operation, synthetic media was replaced by real winery wastewater, with a COD loading of 6 kg COD/(m3 day). The decrease of the organic load implied a reduction of the aggregate diameter and a density increase up to 13.2 g L−1. The effluent was free of organic matter and the solids concentration in the reactor reached 6 g VSS L−1.

Experience from start-ups of the first ANITA Mox Plants

2013 ◽  
Vol 67 (12) ◽  
pp. 2677-2684 ◽  
Author(s):  
M. Christensson ◽  
S. Ekström ◽  
A. Andersson Chan ◽  
E. Le Vaillant ◽  
R. Lemaire
Keyword(s):  
Nitrogen Removal ◽  
Removal Rate ◽  
Treatment Plant ◽  
Ammonia Removal ◽  
Nitrogen Loading ◽  
Biofilm Reactor ◽  
Process Conditions ◽  
N2o Emissions ◽  
N Removal ◽  
Start Up

ANITA™ Mox is a new one-stage deammonification Moving-Bed Biofilm Reactor (MBBR) developed for partial nitrification to nitrite and autotrophic N-removal from N-rich effluents. This deammonification process offers many advantages such as dramatically reduced oxygen requirements, no chemical oxygen demand requirement, lower sludge production, no pre-treatment or requirement of chemicals and thereby being an energy and cost efficient nitrogen removal process. An innovative seeding strategy, the ‘BioFarm concept’, has been developed in order to decrease the start-up time of new ANITA Mox installations. New ANITA Mox installations are started with typically 3–15% of the added carriers being from the ‘BioFarm’, with already established anammox biofilm, the rest being new carriers. The first ANITA Mox plant, started up in 2010 at Sjölunda wastewater treatment plant (WWTP) in Malmö, Sweden, proved this seeding concept, reaching an ammonium removal rate of 1.2 kgN/m3 d and approximately 90% ammonia removal within 4 months from start-up. This first ANITA Mox plant is also the BioFarm used for forthcoming installations. Typical features of this first installation were low energy consumption, 1.5 kW/NH4-N-removed, low N2O emissions, <1% of the reduced nitrogen and a very stable and robust process towards variations in loads and process conditions. The second ANITA Mox plant, started up at Sundets WWTP in Växjö, Sweden, reached full capacity with more than 90% ammonia removal within 2 months from start-up. By applying a nitrogen loading strategy to the reactor that matches the capacity of the seeding carriers, more than 80% nitrogen removal could be obtained throughout the start-up period.

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